##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936919_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1164910 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.916589264406692 33.0 31.0 34.0 30.0 34.0 2 32.031595573907 33.0 31.0 34.0 30.0 34.0 3 32.0380844872136 34.0 31.0 34.0 30.0 34.0 4 35.604456996677854 37.0 35.0 37.0 33.0 37.0 5 35.63184452017752 37.0 35.0 37.0 33.0 37.0 6 35.690024122035176 37.0 35.0 37.0 33.0 37.0 7 35.640027984994546 37.0 35.0 37.0 33.0 37.0 8 35.6080006180735 37.0 35.0 37.0 33.0 37.0 9 37.25415010601677 39.0 37.0 39.0 34.0 39.0 10-11 37.14100016310273 39.0 37.0 39.0 34.0 39.0 12-13 37.05835429346473 39.0 37.0 39.0 33.0 39.0 14-15 38.248289996652105 40.0 38.0 41.0 33.5 41.0 16-17 38.17888334721137 40.0 38.0 41.0 33.0 41.0 18-19 38.153320428187584 40.0 38.0 41.0 33.5 41.0 20-21 38.10921358731576 40.0 38.0 41.0 33.0 41.0 22-23 37.984081173652896 40.0 38.0 41.0 33.0 41.0 24-25 37.817740426299025 40.0 38.0 41.0 33.0 41.0 26-27 37.779429312135704 40.0 37.5 41.0 33.0 41.0 28-29 37.84446867139951 40.0 38.0 41.0 33.0 41.0 30-31 37.70729884712124 40.0 37.5 41.0 33.0 41.0 32-33 37.52421603385669 40.0 37.0 41.0 32.5 41.0 34-35 37.33252783476835 40.0 37.0 41.0 32.0 41.0 36-37 37.18773939617653 40.0 36.5 41.0 31.0 41.0 38-39 37.030740143015336 40.0 36.0 41.0 31.0 41.0 40-41 36.834156286751764 39.0 36.0 41.0 31.0 41.0 42-43 36.63489969182169 39.0 36.0 41.0 30.0 41.0 44-45 36.39508631568104 39.0 36.0 41.0 30.0 41.0 46-47 36.05601977835198 39.0 35.0 41.0 29.5 41.0 48-49 35.71954700363118 39.0 35.0 40.0 28.0 41.0 50-51 35.100448961722364 38.5 34.5 39.5 27.0 40.5 52-53 35.428430951747345 38.5 35.0 40.0 28.0 40.5 54-55 35.75999562197938 39.0 35.0 41.0 28.0 41.0 56-57 35.667210771647596 39.0 35.0 41.0 28.0 41.0 58-59 35.461909074520776 39.0 35.0 41.0 27.5 41.0 60-61 35.193711531362936 39.0 35.0 40.5 26.5 41.0 62-63 34.92672223605257 38.0 34.5 40.0 26.0 41.0 64-65 34.63496235760702 38.0 34.0 40.0 26.0 41.0 66-67 34.281930363719084 37.5 34.0 40.0 25.5 41.0 68-69 33.85582963490742 37.0 34.0 39.5 24.0 41.0 70-71 33.49772471693092 36.0 33.5 39.0 23.5 41.0 72-73 33.12100634383772 36.0 33.0 39.0 22.5 40.5 74-75 32.68387429071774 35.5 33.0 38.5 22.0 40.0 76-77 32.24784790241306 35.0 32.0 37.0 20.5 39.5 78-79 31.79770926509344 35.0 32.0 37.0 19.5 39.0 80-81 31.36418607446069 35.0 31.5 36.5 18.0 39.0 82-83 30.933336480929857 35.0 31.0 36.0 15.5 37.5 84-85 30.553123846477412 34.5 31.0 35.5 11.0 37.0 86-87 30.23029418581693 34.0 31.0 35.0 7.0 37.0 88-89 29.85395738726597 34.0 30.5 35.0 4.5 36.0 90-91 29.4998240207398 34.0 30.0 35.0 2.0 36.0 92-93 29.14337244937377 34.0 30.0 35.0 2.0 36.0 94-95 28.754345400073824 34.0 29.0 35.0 2.0 35.0 96-97 28.322743387901212 34.0 29.0 35.0 2.0 35.0 98-99 27.748007142182658 34.0 29.0 35.0 2.0 35.0 100-101 25.6475860796113 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4101156027920183 1101 2 0.37092234733579943 1101 3 0.4351610997048816 1101 4 0.29403772712971943 1101 5 0.35734647677228537 1101 6 0.31320937566991347 1101 7 0.32727683514933403 1101 8 0.23247535706648392 1101 9 0.32661144919890006 1101 10-11 0.31889732860190634 1101 12-13 0.5357273449741626 1101 14-15 0.7572401528158181 1101 16-17 0.7554006469332535 1101 18-19 0.7912130755502176 1101 20-21 0.6763482007420265 1101 22-23 0.6672452404976852 1101 24-25 0.730606741333041 1101 26-27 0.7479142135213763 1101 28-29 0.7924778327125566 1101 30-31 0.6617860943936904 1101 32-33 0.671226737804659 1101 34-35 0.6249985124134341 1101 36-37 0.6307292664154289 1101 38-39 0.820824085543066 1101 40-41 0.7400614208354952 1101 42-43 0.8437042774854717 1101 44-45 0.8200691453838189 1101 46-47 0.8985038516306219 1101 48-49 0.9265589188254566 1101 50-51 1.0234682684594674 1101 52-53 1.0070235533623162 1101 54-55 0.949350498232775 1101 56-57 0.9430025276483605 1101 58-59 0.9590476382852771 1101 60-61 0.8620125462052144 1101 62-63 0.8086979291894707 1101 64-65 0.8028218513652305 1101 66-67 0.8692029972820876 1101 68-69 0.9918258296546938 1101 70-71 0.8718430707411855 1101 72-73 0.9087101436176894 1101 74-75 0.9075349821706453 1101 76-77 0.9680704896580963 1101 78-79 0.9639387919977302 1101 80-81 1.0071508580545618 1101 82-83 0.9160723652766976 1101 84-85 1.0949662391520398 1101 86-87 1.033318824084244 1101 88-89 1.0806908677914002 1101 90-91 0.8203273066501886 1101 92-93 0.6543934315326183 1101 94-95 0.9451345364874442 1101 96-97 0.986702015605335 1101 98-99 1.1919577482476917 1101 100-101 1.2429855694311627 1102 1 0.34423774181554734 1102 2 0.3956545765219559 1102 3 0.2448109663540201 1102 4 0.1261557614988149 1102 5 0.05772234852460656 1102 6 0.2062401202478341 1102 7 0.05200492699957948 1102 8 0.1113640616874676 1102 9 0.2106568736704446 1102 10-11 0.17665502465513327 1102 12-13 0.18308916751583126 1102 14-15 0.32385578075041366 1102 16-17 0.20658060470461237 1102 18-19 0.31425525516831243 1102 20-21 0.2867606583647557 1102 22-23 0.24254892616863089 1102 24-25 0.42918739174310616 1102 26-27 0.3870955842451096 1102 28-29 0.48307742308153223 1102 30-31 0.26053613220337724 1102 32-33 0.39437464547739864 1102 34-35 0.2518833036013959 1102 36-37 0.4145072242890109 1102 38-39 0.3161370750046828 1102 40-41 0.48744229598286637 1102 42-43 0.21111222263066054 1102 44-45 0.5099139327056363 1102 46-47 0.2603328252897015 1102 48-49 0.28944656373217015 1102 50-51 0.24300417757993387 1102 52-53 0.23733791787969238 1102 54-55 0.3266484145800135 1102 56-57 0.2715469815843363 1102 58-59 0.31522202108342157 1102 60-61 0.45483941399508154 1102 62-63 0.596021131960299 1102 64-65 0.6027754019469427 1102 66-67 0.2690492205028363 1102 68-69 0.1101047883115811 1102 70-71 0.3723060003852581 1102 72-73 0.429281568811696 1102 74-75 0.3490730350569251 1102 76-77 0.45220780470788213 1102 78-79 0.37467959416110475 1102 80-81 0.5295971222314542 1102 82-83 0.5183787735928149 1102 84-85 0.4793617726371693 1102 86-87 0.4319570495217704 1102 88-89 0.41390355780029253 1102 90-91 0.37503418683120415 1102 92-93 0.3626142484513295 1102 94-95 0.418316950932379 1102 96-97 0.5624208255684557 1102 98-99 0.6463324942274369 1102 100-101 0.9954948288932286 1103 1 0.19665600595811839 1103 2 0.2638068059509635 1103 3 0.0820363193737812 1103 4 0.11622805473845688 1103 5 0.12814101286128476 1103 6 0.11175677186991351 1103 7 0.07475193234571975 1103 8 0.0989201603253278 1103 9 0.30853153990270954 1103 10-11 0.2065807270009543 1103 12-13 0.07062257011970985 1103 14-15 0.15196414725244267 1103 16-17 0.33096692495426794 1103 18-19 0.37388762074596826 1103 20-21 0.12149960747983357 1103 22-23 0.10336386188099311 1103 24-25 0.2910040181205815 1103 26-27 0.43091270587139974 1103 28-29 0.4609877072779014 1103 30-31 0.48351881123771534 1103 32-33 0.3886794563133549 1103 34-35 0.31636759219437494 1103 36-37 0.4670271900830869 1103 38-39 0.2481758848783855 1103 40-41 0.44903916000400557 1103 42-43 0.557164748623471 1103 44-45 0.45838842515308187 1103 46-47 0.5643446506466461 1103 48-49 0.6408433341450817 1103 50-51 0.5388843480309333 1103 52-53 0.5983830857425261 1103 54-55 0.5126418964443644 1103 56-57 0.7521146648427504 1103 58-59 0.7145425521086253 1103 60-61 0.6794901998667839 1103 62-63 0.7273280205682298 1103 64-65 0.7083076549398797 1103 66-67 0.775129450121959 1103 68-69 0.6536680863105104 1103 70-71 0.7901809696905104 1103 72-73 0.7485594905300204 1103 74-75 0.6364573464148222 1103 76-77 0.4506903733616596 1103 78-79 0.5801224406226062 1103 80-81 0.6038541370796224 1103 82-83 0.6402645694917553 1103 84-85 0.558789109338516 1103 86-87 0.5445553803472869 1103 88-89 0.6372709328885868 1103 90-91 0.5976602385030816 1103 92-93 0.5004248078586286 1103 94-95 0.507902226544541 1103 96-97 0.4531178968846312 1103 98-99 0.49936534217330575 1103 100-101 0.8209185886445169 1104 1 0.30648067562768233 1104 2 0.21443177908052746 1104 3 0.23745684317709603 1104 4 0.21571735469169795 1104 5 0.2087173281431376 1104 6 0.2697560969225208 1104 7 0.2767521963893955 1104 8 0.31439996840286 1104 9 0.1191783594128708 1104 10-11 0.31147664471063763 1104 12-13 0.2872209494089475 1104 14-15 0.5399443766965035 1104 16-17 0.4153616010118526 1104 18-19 0.36203114636828815 1104 20-21 0.3845550265959119 1104 22-23 0.481914231637262 1104 24-25 0.49548891797992667 1104 26-27 0.5014547905913744 1104 28-29 0.49833733268970803 1104 30-31 0.43599228036458015 1104 32-33 0.4512245178024372 1104 34-35 0.37602852814932675 1104 36-37 0.514280355848868 1104 38-39 0.5069255657621952 1104 40-41 0.500758240355843 1104 42-43 0.6275185024761711 1104 44-45 0.3628694823017966 1104 46-47 0.3325763151148493 1104 48-49 0.3345496209338066 1104 50-51 0.4613277945542791 1104 52-53 0.33871800270382835 1104 54-55 0.35447478570999635 1104 56-57 0.5113328900963694 1104 58-59 0.4652016150274889 1104 60-61 0.3006980437142417 1104 62-63 0.2976142416351948 1104 64-65 0.28160450661847847 1104 66-67 0.299269662642871 1104 68-69 0.24974764640003144 1104 70-71 0.15453423578528458 1104 72-73 0.2206465732011793 1104 74-75 0.2131378760088367 1104 76-77 0.26800258370784036 1104 78-79 0.27995937272419624 1104 80-81 0.35392499894635066 1104 82-83 0.35164333025942796 1104 84-85 0.31584666591482247 1104 86-87 0.37638752009411647 1104 88-89 0.35890611659488414 1104 90-91 0.3194523645987779 1104 92-93 0.2654392484607868 1104 94-95 0.32977603877600714 1104 96-97 0.18709312776115183 1104 98-99 0.37897987644629083 1104 100-101 0.4059010852878515 1105 1 0.28371762428106706 1105 2 0.186482493284565 1105 3 0.2745917193436078 1105 4 0.3161222375557031 1105 5 0.2845660069648446 1105 6 0.27376245370016505 1105 7 0.2501046031671592 1105 8 0.3288320005881289 1105 9 0.3377077595094846 1105 10-11 0.38397494700294743 1105 12-13 0.27564059941278884 1105 14-15 0.33979665862566577 1105 16-17 0.47334479428916865 1105 18-19 0.3816333110330987 1105 20-21 0.41208612790063626 1105 22-23 0.3707772692155302 1105 24-25 0.42675917670824504 1105 26-27 0.37072546470383116 1105 28-29 0.3110298854119904 1105 30-31 0.25429881986458014 1105 32-33 0.25422555159556737 1105 34-35 0.31766332147238074 1105 36-37 0.3139282127700369 1105 38-39 0.407689125837706 1105 40-41 0.48469827568683144 1105 42-43 0.49801549327470696 1105 44-45 0.28142618573852474 1105 46-47 0.48345293902184494 1105 48-49 0.5912868951270198 1105 50-51 0.5776563587486407 1105 52-53 0.743597833003065 1105 54-55 0.7826735353570413 1105 56-57 0.7736391122242026 1105 58-59 0.8029742692155182 1105 60-61 0.7220499557648097 1105 62-63 0.6698327430318542 1105 64-65 0.7362030191698494 1105 66-67 0.7221192912474379 1105 68-69 0.9079784366333783 1105 70-71 0.9388866215604779 1105 72-73 0.8202442573178743 1105 74-75 0.7973882826419114 1105 76-77 0.5919688489142771 1105 78-79 0.8020699442507784 1105 80-81 0.8111642345461725 1105 82-83 0.9758665268971498 1105 84-85 0.9148016714104905 1105 86-87 0.9222587650097473 1105 88-89 1.0498876133313182 1105 90-91 0.8454528453242496 1105 92-93 1.0101525517215535 1105 94-95 1.0302695991548916 1105 96-97 1.0181869649072297 1105 98-99 0.8642465740723004 1105 100-101 1.2123157366802069 1106 1 0.5442813997435536 1106 2 0.4090260296942816 1106 3 0.3743167525453863 1106 4 0.4232358343832985 1106 5 0.3075809150286659 1106 6 0.4024843257190227 1106 7 0.4525736927838935 1106 8 0.3540803124416314 1106 9 0.5807148113790745 1106 10-11 0.590723791955341 1106 12-13 0.5129257231478519 1106 14-15 0.6743583016925712 1106 16-17 0.7096142243409815 1106 18-19 0.8443743746121584 1106 20-21 0.7187582814331179 1106 22-23 0.8349100014876498 1106 24-25 0.6516202904172985 1106 26-27 0.7601832002493794 1106 28-29 0.5332703971869321 1106 30-31 0.5755198969114019 1106 32-33 0.7073240720123266 1106 34-35 0.7348627752798293 1106 36-37 0.6481007871700655 1106 38-39 0.9894272152860921 1106 40-41 0.8064356169262439 1106 42-43 0.9375134450526446 1106 44-45 0.9402634689917377 1106 46-47 0.9367029717559916 1106 48-49 1.1397259441534047 1106 50-51 1.1750357568145233 1106 52-53 1.1229659705238788 1106 54-55 1.0823143950491527 1106 56-57 1.0510768705901157 1106 58-59 1.1351715719221644 1106 60-61 1.0649092363618777 1106 62-63 1.2556336072132268 1106 64-65 0.878286032955593 1106 66-67 1.126950850701995 1106 68-69 1.0574051215064557 1106 70-71 1.199182912314491 1106 72-73 1.0904393528080405 1106 74-75 1.1175562555567424 1106 76-77 1.2074287168553894 1106 78-79 1.220717182424405 1106 80-81 1.4475271907198284 1106 82-83 1.3014328275649305 1106 84-85 1.4906158163011618 1106 86-87 1.4432104868256523 1106 88-89 1.519486012164112 1106 90-91 1.4213343290226526 1106 92-93 1.5001546092962315 1106 94-95 1.6019237021458572 1106 96-97 1.4894650928883735 1106 98-99 1.471232975374182 1106 100-101 1.524391829532938 1107 1 0.09937013910537829 1107 2 0.21740527617166805 1107 3 0.21470312630573218 1107 4 0.2680767599922831 1107 5 0.1963062098496664 1107 6 0.2340741318287911 1107 7 0.2940760490073657 1107 8 0.30250598003828344 1107 9 0.3120678228905689 1107 10-11 0.37101122777463047 1107 12-13 0.2821142913611112 1107 14-15 0.1956651265349052 1107 16-17 0.4140687761896231 1107 18-19 0.3845262982752047 1107 20-21 0.48565392769481264 1107 22-23 0.4590958735247881 1107 24-25 0.5481715294860692 1107 26-27 0.5792182036488995 1107 28-29 0.3114595046354083 1107 30-31 0.3619780592915376 1107 32-33 0.4558786871624818 1107 34-35 0.5439664725578623 1107 36-37 0.5757541761462193 1107 38-39 0.5208880738423503 1107 40-41 0.5632663205109836 1107 42-43 0.6728966537366716 1107 44-45 0.7763148604530627 1107 46-47 0.6548775432984328 1107 48-49 0.46564205790859603 1107 50-51 0.34852882881413905 1107 52-53 0.33535665816602034 1107 54-55 0.40508887750056743 1107 56-57 0.500473161589575 1107 58-59 0.5021117637023451 1107 60-61 0.48531342832962565 1107 62-63 0.2740848298704819 1107 64-65 0.5662845324749739 1107 66-67 0.46927612676697805 1107 68-69 0.49603077503611104 1107 70-71 0.6509143262830221 1107 72-73 0.5697092752050565 1107 74-75 0.6195373588789082 1107 76-77 0.4285714266296168 1107 78-79 0.6934693921165653 1107 80-81 0.6142352769036883 1107 82-83 0.6328327290958882 1107 84-85 0.6233450498837918 1107 86-87 0.7225413662479632 1107 88-89 0.5649177520182853 1107 90-91 0.6630852023492615 1107 92-93 0.6429440965538689 1107 94-95 0.5321677646971423 1107 96-97 0.4429431600817715 1107 98-99 0.6705765151017538 1107 100-101 1.0148215765612854 1108 1 -0.11596009995624357 1108 2 0.03607577507442983 1108 3 0.0182492393696414 1108 4 0.029182152958320273 1108 5 0.039084839954725226 1108 6 -0.11829220922727757 1108 7 -0.14318338431366584 1108 8 -0.23536130289338075 1108 9 -0.22511587198057725 1108 10-11 -0.17724970089528824 1108 12-13 -0.3105526730806645 1108 14-15 0.04069066398660226 1108 16-17 0.04072676853809298 1108 18-19 -0.045609836733440545 1108 20-21 0.10431277452835985 1108 22-23 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58-59 -1.4322984981236928 2316 60-61 -1.551838979933784 2316 62-63 -1.2835757954900018 2316 64-65 -1.3976211679076513 2316 66-67 -1.4069799964529182 2316 68-69 -1.1579961305749151 2316 70-71 -1.4704322540560568 2316 72-73 -1.2617285340023159 2316 74-75 -1.39906545028785 2316 76-77 -1.7474312379357215 2316 78-79 -2.1048356031310824 2316 80-81 -2.088734332428789 2316 82-83 -2.0617414684881297 2316 84-85 -1.5061865390257658 2316 86-87 -1.3235442410373501 2316 88-89 -1.789355300939949 2316 90-91 -1.7449129617002264 2316 92-93 -2.121597281383856 2316 94-95 -2.547373978805947 2316 96-97 -2.599596869325932 2316 98-99 -2.357467222763507 2316 100-101 -2.128831396297514 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2892.0 3 793.0 4 767.0 5 847.0 6 911.0 7 1053.0 8 1302.0 9 1614.0 10 1925.0 11 2574.0 12 3345.0 13 4451.0 14 4980.0 15 6314.0 16 8079.0 17 10940.0 18 9834.0 19 6120.0 20 5420.0 21 5650.0 22 6052.0 23 6839.0 24 7661.0 25 8869.0 26 10305.0 27 12398.0 28 14827.0 29 17967.0 30 22365.0 31 28424.0 32 36507.0 33 49395.0 34 68668.0 35 99830.0 36 155462.0 37 241781.0 38 247014.0 39 50709.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.50581305956335 20.06899223407883 15.11498562518629 22.31020908117153 2 14.469957619982196 20.828335149292464 41.86083680771571 22.840870423009633 3 18.231807365536437 26.28621990086507 30.854673688008454 24.627299045590043 4 11.794477275169228 17.505963253465133 39.87596432792522 30.823595143440418 5 12.655579016480226 38.02448701785866 35.41585563195395 13.904078333707162 6 29.06200533826484 38.06108927414379 18.949293358792925 13.927612028798444 7 26.39313224754917 33.66077022438451 22.66525035171535 17.280847176350978 8 24.813706653284708 35.15968983188916 21.299997593997368 18.726605920828764 9 25.7114593252911 17.170337698233986 21.86727562130218 35.250927355172735 10-11 23.28517606293474 27.366025620091815 29.15368273780571 20.195115579167734 12-13 24.64025639813888 25.1585947938461 29.20373942593969 20.99740938207533 14-15 21.86706112991724 26.289348761853127 27.791227775460133 24.05236233276951 16-17 20.538892849208917 29.58900471929782 28.432491009787075 21.439611421706193 18-19 20.43497312832674 28.073316063289127 31.215991545411192 20.275719262972938 20-21 21.21573055811722 27.249196887975806 31.09081920218407 20.444253351722903 22-23 20.906496111611045 27.092137958880368 31.071631834748302 20.929734094760285 24-25 20.791720729229347 27.39218518408759 31.05491038392043 20.761183702762633 26-27 20.573079167827995 27.46001840662845 31.062323572724004 20.90457885281955 28-29 20.32052119054655 27.38621291568451 31.314298378839005 20.978967514929938 30-31 20.604845445515686 27.3438254859767 31.16907098113465 20.882258087372957 32-33 20.418122836144324 27.401251740317566 31.08415449983209 21.096470923706022 34-35 20.844516167264562 27.493754074130056 30.828163822326278 20.833565936279104 36-37 20.924995394261916 27.067267824635827 30.88515629663444 21.12258048446782 38-39 20.50256137619056 27.635550673335533 30.901332156103738 20.96055579437017 40-41 20.826899842847453 27.485030994570742 30.89187827571145 20.796190886870352 42-43 20.56214688658926 27.50963646632122 30.580077693003123 21.348138954086398 44-45 20.727786473597988 27.658419593722694 30.424334555042392 21.189459377636926 46-47 20.886138452316956 27.82047604898918 29.971223750581792 21.322161748112066 48-49 20.8789090092446 27.87404259412174 29.84882036774863 21.39822802888503 50-51 20.84829365404769 27.963302689272833 29.737890458467376 21.450513198212104 52-53 21.04003929731995 28.236146320685368 29.432713644826425 21.291100737168257 54-55 20.92769400818124 28.038142482078303 29.563730116947262 21.47043339279319 56-57 20.933219619281232 28.330000927576858 29.289613543996346 21.447165909145564 58-59 21.027388788874077 28.03696215317383 29.400303365536573 21.535345692415518 60-61 21.082245226178127 28.02560353175856 29.66380870786336 21.228342534199953 62-63 20.849003487696432 28.010956546162696 29.87797571883008 21.26206424731079 64-65 20.852471686973324 28.004895067360852 29.666598679619987 21.476034566045836 66-67 20.75803374555375 28.30289567877215 29.155081288766453 21.783989286907644 68-69 20.779672531867956 28.133598271574296 29.48961024962284 21.59711894693491 70-71 20.899714975200396 27.97033921772091 29.48347358465374 21.646472222424958 72-73 20.915419613472896 27.922972767603778 29.35761699146288 21.803990627460443 74-75 20.73714219472507 28.334442474228577 29.20845981117413 21.71995551987223 76-77 20.818107257416944 28.12941484633651 29.378003169027693 21.674474727218858 78-79 20.87770309446604 28.035089226276117 29.20617604741555 21.88103163184229 80-81 20.79014567903037 28.30390654419901 29.026011137509034 21.879936639261587 82-83 21.002406827763213 28.198323638450145 29.132408167752978 21.666861366033665 84-85 20.836051612005466 28.127967469129732 29.046677658605386 21.98930326025942 86-87 20.75679641006611 28.103009593314916 29.323777814428453 21.81641618219052 88-89 20.940953385789427 27.90554809974104 29.041691081833914 22.11180743263562 90-91 20.607690077630917 27.89653506275971 29.459160555334847 22.036614304274533 92-93 20.943715223100494 27.935305624354072 29.36740298979319 21.75357616275224 94-95 20.674002561950314 27.73959862778436 29.298547970414713 22.287850839850613 96-97 20.680584298220477 27.837339596596905 29.272393966954613 22.209682138228004 98-99 20.49475097425425 27.850649875285143 29.15650296444896 22.49809618601165 100-101 20.4613782043018 27.70482349159529 29.363849511050145 22.46994879305277 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 217.0 1 180.5 2 155.0 3 173.5 4 165.0 5 188.5 6 302.5 7 621.5 8 1060.0 9 1274.5 10 1189.5 11 1133.5 12 1253.5 13 1377.0 14 1491.5 15 1571.5 16 1635.5 17 1754.0 18 1888.0 19 2070.5 20 2337.5 21 2729.0 22 3239.0 23 4041.0 24 5004.0 25 6208.5 26 8342.0 27 10770.5 28 12435.0 29 14371.0 30 17192.0 31 20491.0 32 24022.0 33 27911.0 34 32185.0 35 35685.0 36 40436.5 37 44947.0 38 45433.5 39 44670.5 40 44125.5 41 43573.5 42 42865.0 43 43047.5 44 43974.5 45 44171.0 46 42586.0 47 41161.0 48 43244.0 49 42182.5 50 37458.5 51 34424.0 52 31620.5 53 29695.0 54 27955.5 55 25011.0 56 22207.5 57 19589.5 58 17374.0 59 15106.5 60 12600.5 61 10158.0 62 7814.0 63 5817.0 64 4235.0 65 3149.5 66 2238.0 67 1596.0 68 1136.5 69 768.0 70 532.0 71 390.0 72 280.0 73 215.5 74 164.5 75 119.0 76 82.0 77 44.0 78 33.0 79 28.0 80 23.0 81 15.5 82 11.0 83 7.0 84 5.5 85 5.0 86 3.5 87 4.0 88 4.5 89 4.0 90 4.0 91 3.0 92 1.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.061893193465589615 2 0.09906344696156784 3 0.07210857491136655 4 0.13176983629636624 5 0.10927882840734476 6 0.1061026173695822 7 0.11391437965164691 8 0.09906344696156784 9 0.1189791486037548 10-11 0.11090985569700663 12-13 0.09399867800945996 14-15 0.09738949790112542 16-17 0.11018018559373687 18-19 0.08970650093140242 20-21 0.07249487084839172 22-23 0.07446927230429819 24-25 0.06433973440008241 26-27 0.056270441493334246 28-29 0.06918989449828743 30-31 0.0497463323346868 32-33 0.05245040389386305 34-35 0.047299791400194004 36-37 0.05163489024903211 38-39 0.05103398545810405 40-41 0.06476895210788816 42-43 0.06116352336231984 44-45 0.04785777442034149 46-47 0.03502416495694947 48-49 0.0429646925513559 50-51 0.02588182778068692 52-53 0.03897296786876239 54-55 0.0462696689014602 56-57 0.050304315354834275 58-59 0.054768179516014114 60-61 0.053008386914010525 62-63 0.05790146878299612 64-65 0.040561073387643684 66-67 0.06240825471495652 68-69 0.05266501274776592 70-71 0.023564052158535853 72-73 0.019829858100625798 74-75 0.029616021838596976 76-77 0.04420942390399259 78-79 0.06309500304744572 80-81 0.04004601213827678 82-83 0.06197903700715077 84-85 0.06210780231949249 86-87 0.0657561528358414 88-89 0.08721703822612906 90-91 0.0641680473169601 92-93 0.07579984719849601 94-95 0.08180889510777657 96-97 0.09326900790619018 98-99 0.12498819651303535 100-101 0.11983758401936631 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1164910.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.96012349070594 #Duplication Level Percentage of deduplicated Percentage of total 1 81.88269516644905 49.096965139321156 2 11.036019373923924 13.234421690126036 3 3.0494780219368796 5.48541236332587 4 1.2710182892340673 3.0484165432568195 5 0.6510729344148799 1.9519206774486246 6 0.3838218130465132 1.380840198521735 7 0.2599747155811183 1.0911681235493504 8 0.19706387168337414 0.9452779265353395 9 0.13605135408850502 0.7341890393002068 >10 0.9656728012328762 11.33120025144309 >50 0.10972897134098179 4.5476372154165 >100 0.056111128040565755 5.720475441406269 >500 7.175327929579968E-4 0.31751235897301755 >1k 4.3051967577479797E-4 0.6435433422111648 >5k 1.4350655859159936E-4 0.4710196891648505 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5474 0.4699075465057386 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3830 0.328780764179207 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2579 0.22139049368620753 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.584354156115064E-5 0.0 18-19 0.0 0.0 0.0 1.716870831223013E-4 0.0 20-21 0.0 0.0 0.0 3.862959370251779E-4 0.0 22-23 0.0 0.0 0.0 7.296701032697805E-4 0.0 24-25 0.0 0.0 0.0 9.872007279532326E-4 0.0 26-27 0.0 0.0 0.0 0.0017168708312230128 0.0 28-29 0.0 0.0 0.0 0.0031332892669819986 0.0 30-31 0.0 0.0 0.0 0.005880282596938819 0.0 32-33 0.0 0.0 0.0 0.009142337176262543 0.0 34-35 0.0 0.0 0.0 0.013048218317294899 0.0 36-37 0.0 0.0 0.0 0.01845636143564739 0.0 38-39 0.0 0.0 0.0 0.02571014069756462 0.0 40-41 0.0 0.0 0.0 0.04086152578310771 0.0 42-43 0.0 0.0 0.0 0.055454927848503324 0.0 44-45 0.0 0.0 0.0 0.07146474834965791 0.0 46-47 0.0 0.0 0.0 0.10588800851567932 0.0 48-49 0.0 0.0 0.0 0.12614708432411087 0.0 50-51 0.0 0.0 0.0 0.14842348335922947 0.0 52-53 0.0 0.0 0.0 0.17482037238928327 0.0 54-55 0.0 0.0 0.0 0.21134679932355288 0.0 56-57 0.0 0.0 0.0 0.30749156587204163 0.0 58-59 0.0 0.0 0.0 0.39560996128456277 0.0 60-61 0.0 0.0 0.0 0.4588766514151308 0.0 62-63 0.0 0.0 0.0 0.5424882608956916 0.0 64-65 0.0 0.0 0.0 0.6226232069430256 0.0 66-67 0.0 0.0 0.0 0.7461520632495214 0.0 68-69 0.0 0.0 0.0 0.9080529826338515 0.0 70-71 0.0 0.0 0.0 0.9892180511799195 0.0 72-73 0.0 0.0 0.0 1.0830879638770377 0.0 74-75 0.0 0.0 0.0 1.2617283738657923 0.0 76-77 0.0 0.0 0.0 1.463289009451374 0.0 78-79 0.0 0.0 0.0 1.6454919264149162 0.0 80-81 0.0 0.0 0.0 1.7763174837541098 0.0 82-83 0.0 0.0 0.0 1.9912267900524503 0.0 84-85 0.0 0.0 0.0 2.3370904190023265 0.0 86-87 0.0 4.292177078057532E-5 0.0 2.6822243778489323 0.0 88-89 0.0 8.584354156115064E-5 0.0 3.073928457992463 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5900 0.0 33.747383 1 GGTATCA 3760 0.0 32.354164 1 ATCAACG 7085 0.0 27.698162 3 CAACGCA 7160 0.0 27.47439 5 TCAACGC 7160 0.0 27.47439 4 AACGCAG 7315 0.0 27.022139 6 TATCAAC 7265 0.0 27.015387 2 GTACATG 5020 0.0 24.422722 1 ACGCAGA 8245 0.0 23.743639 7 ACATGGG 4885 0.0 23.53919 3 GTATAGG 445 0.0 23.493124 1 CGCAGAG 8275 0.0 23.486307 8 TACATGG 5185 0.0 23.46324 2 GAGTACT 4185 0.0 22.421553 12-13 GCAGAGT 8675 0.0 21.691277 9 GTGTAGT 540 0.0 21.120079 1 GTACTTT 4640 0.0 20.325722 14-15 AGAGTAC 6880 0.0 19.992336 10-11 AGTACTT 4615 0.0 19.817694 12-13 GAGTACG 120 0.0047518434 19.800076 1 >>END_MODULE