FastQCFastQC Report
Thu 26 May 2016
SRR936919_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936919_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164910
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66040.5669107484698389No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50190.4308487350954151No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34620.29719034088470353No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16810.14430299336429425No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12960.11125322986325124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56000.030.8753471
GTATCAA82000.028.6161791
ATCAACG97700.023.9194093
TCAACGC98350.023.7603034
CGCGGAA901.9542313E-723.7482382-83
CAACGCA98850.023.6401215
GTACATG46950.023.4720441
TATCAAC99700.023.439582
AACGCAG100450.023.2635736
AGGACCG1652.431505E-623.0285855
CGATCGT953.2814387E-722.50605224-25
GAGTACT59350.022.40776412-13
ACATGGG47150.021.658913
CTCCCGC2205.6439603E-821.5892987
TACATGG50800.021.3182242
GTACTTT62600.021.2837314-15
CTGGTCG3800.021.2484159
ACGCAGA112000.020.6948857
GGACCGA1150.003724916120.6506356
CGCAGAG111950.020.6192768
Produced by FastQC (version 0.11.5)