##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936919_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1164910 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30590431878858 33.0 31.0 34.0 31.0 34.0 2 32.32103166768248 33.0 31.0 34.0 31.0 34.0 3 32.49477727893142 34.0 31.0 34.0 31.0 34.0 4 36.18921461743826 37.0 35.0 37.0 35.0 37.0 5 36.13745782936021 37.0 35.0 37.0 35.0 37.0 6 36.18810981105837 37.0 36.0 37.0 35.0 37.0 7 36.14122550239933 37.0 36.0 37.0 35.0 37.0 8 36.12650075971534 37.0 35.0 37.0 35.0 37.0 9 37.84996265805942 39.0 38.0 39.0 35.0 39.0 10-11 37.77448687023032 39.0 37.0 39.0 35.0 39.0 12-13 37.69734271317097 39.0 37.0 39.0 35.0 39.0 14-15 38.94158003622597 40.0 38.0 41.0 36.0 41.0 16-17 38.87115141942296 40.0 38.0 41.0 35.5 41.0 18-19 38.95035324617352 40.0 38.0 41.0 35.5 41.0 20-21 39.03153333733937 40.0 38.0 41.0 36.0 41.0 22-23 38.96314908447863 40.0 38.0 41.0 35.0 41.0 24-25 38.86362422848117 40.0 38.0 41.0 35.0 41.0 26-27 38.71945214651775 40.0 38.0 41.0 35.0 41.0 28-29 38.560223965799935 40.0 38.0 41.0 34.0 41.0 30-31 38.409725644041174 40.0 38.0 41.0 34.0 41.0 32-33 38.37726090427587 40.0 38.0 41.0 34.0 41.0 34-35 38.283419749165176 40.0 38.0 41.0 34.0 41.0 36-37 38.108846176957876 40.0 38.0 41.0 34.0 41.0 38-39 37.961727515430375 40.0 37.5 41.0 33.5 41.0 40-41 37.79361109441931 40.0 37.0 41.0 33.0 41.0 42-43 37.55263024611344 40.0 37.0 41.0 33.0 41.0 44-45 37.149050141212626 40.0 37.0 41.0 32.0 41.0 46-47 36.85210230833283 40.0 37.0 41.0 31.0 41.0 48-49 36.49732640289808 40.0 36.5 41.0 30.5 41.0 50-51 36.239191868899745 39.5 36.0 41.0 30.0 41.0 52-53 36.010834313380435 39.0 36.0 41.0 29.5 41.0 54-55 35.79828613369273 39.0 35.0 40.0 29.0 41.0 56-57 35.5534311663562 39.0 35.0 40.0 28.0 41.0 58-59 35.254013185567985 38.5 35.0 40.0 28.0 41.0 60-61 34.943949317973065 38.0 34.5 40.0 27.0 41.0 62-63 34.610839463992924 38.0 34.0 40.0 26.0 41.0 64-65 34.24742340610004 37.5 34.0 40.0 26.0 41.0 66-67 33.88402537535089 37.0 34.0 39.5 25.5 41.0 68-69 33.457650805641634 36.0 33.0 39.0 24.5 40.0 70-71 32.9924607909624 36.0 32.5 39.0 23.5 40.0 72-73 32.658618262355034 35.0 32.0 38.0 22.5 40.0 74-75 32.20507249487085 35.0 32.0 37.5 21.5 39.5 76-77 30.81493892232018 34.0 30.5 36.0 20.0 38.5 78-79 31.51222798327768 35.0 31.5 36.0 20.5 39.0 80-81 31.320165077130422 35.0 31.0 36.0 20.0 38.5 82-83 30.97546634503953 34.5 31.0 36.0 18.5 37.0 84-85 30.59152037496459 34.0 31.0 35.0 17.5 37.0 86-87 30.235574422058356 34.0 31.0 35.0 12.0 36.5 88-89 29.88195439991072 34.0 30.5 35.0 7.0 36.0 90-91 29.496198418761963 34.0 30.0 35.0 3.5 36.0 92-93 29.07518821196487 34.0 29.5 35.0 2.0 35.0 94-95 28.624653406700947 34.0 29.0 35.0 2.0 35.0 96-97 28.00459091260269 34.0 29.0 35.0 2.0 35.0 98-99 27.354903383093976 33.0 27.5 35.0 2.0 35.0 100-101 24.364854366431743 30.5 21.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2768261511116421 1101 2 0.1063421261932973 1101 3 0.12019677504485315 1101 4 0.16152139407724064 1101 5 0.18309769804678666 1101 6 0.13941278744935204 1101 7 0.27306183112446547 1101 8 0.275106160131692 1101 9 0.24899500865002722 1101 10-11 0.28167812590074703 1101 12-13 0.27219164404621665 1101 14-15 0.40656747762041334 1101 16-17 0.38499752796571585 1101 18-19 0.33676946441334366 1101 20-21 0.3150123990029243 1101 22-23 0.3038105963157136 1101 24-25 0.320885824096905 1101 26-27 0.2987108208424871 1101 28-29 0.2744653319978241 1101 30-31 0.3171477231106934 1101 32-33 0.42053780452045686 1101 34-35 0.44879220635558426 1101 36-37 0.42377773694315835 1101 38-39 0.37838440336398094 1101 40-41 0.26516899732422416 1101 42-43 0.44312521279001515 1101 44-45 0.5555508555984829 1101 46-47 0.5213930236738662 1101 48-49 0.37967384431726714 1101 50-51 0.4804503563476956 1101 52-53 0.3726474960083834 1101 54-55 0.33706387204751564 1101 56-57 0.3063844371677007 1101 58-59 0.25326993981493473 1101 60-61 0.24425157960104826 1101 62-63 0.31959020113187364 1101 64-65 0.2189007151849296 1101 66-67 0.221663715391756 1101 68-69 0.10417401945201021 1101 70-71 0.312630634229599 1101 72-73 0.3638909312212206 1101 74-75 0.20231790768949764 1101 76-77 0.027680555362341153 1101 78-79 0.11860765254382954 1101 80-81 0.31843240468806755 1101 82-83 0.3598121447836853 1101 84-85 0.4418142759491168 1101 86-87 0.19123332453431274 1101 88-89 0.11870504529701265 1101 90-91 0.23632991624901223 1101 92-93 0.3882791732469144 1101 94-95 0.5136906291058949 1101 96-97 0.26673004732599637 1101 98-99 0.5713402102927745 1101 100-101 0.5361117938145021 1102 1 0.17141065654528376 1102 2 0.08613384783612332 1102 3 0.13855020574813182 1102 4 0.1779466176411404 1102 5 0.20993127666503142 1102 6 0.151014711762528 1102 7 0.19799525999896161 1102 8 0.24121616389701472 1102 9 0.2161320452119071 1102 10-11 0.24687356982472153 1102 12-13 0.21133525723382007 1102 14-15 0.40434635405210173 1102 16-17 0.3190148428903328 1102 18-19 0.30192318730292556 1102 20-21 0.28221045257760125 1102 22-23 0.13770490372395017 1102 24-25 0.16700508993186247 1102 26-27 0.18523812534727568 1102 28-29 0.17707227083165833 1102 30-31 0.11681392006804714 1102 32-33 0.29564241132230507 1102 34-35 0.2474734392260629 1102 36-37 0.46352971234721707 1102 38-39 0.38955364578301044 1102 40-41 0.3358790232712323 1102 42-43 0.2844830655752517 1102 44-45 0.40381510411400257 1102 46-47 0.3323569290686379 1102 48-49 0.4321076234746215 1102 50-51 0.3225821343208608 1102 52-53 0.3056183083204047 1102 54-55 0.2111718676403811 1102 56-57 0.24953639873428557 1102 58-59 0.2540946255947034 1102 60-61 0.2616681992446317 1102 62-63 0.37349099616095316 1102 64-65 0.14424387938466765 1102 66-67 0.22557732225514826 1102 68-69 0.1540562096811513 1102 70-71 0.062173528146573176 1102 72-73 0.25386700073124047 1102 74-75 0.1086070754834978 1102 76-77 0.19893792651300757 1102 78-79 0.3832620789543597 1102 80-81 0.2620485127622345 1102 82-83 0.4046222740945531 1102 84-85 0.2961442413519464 1102 86-87 0.2964240470278945 1102 88-89 0.37006558561222036 1102 90-91 0.42578565492514286 1102 92-93 0.3867690456396531 1102 94-95 0.44175748150069793 1102 96-97 0.4622648883879812 1102 98-99 0.4810494598808681 1102 100-101 0.5955906911414708 1103 1 -0.042965610635597784 1103 2 -0.29829805045809366 1103 3 -0.0914694492239363 1103 4 0.07588575576355083 1103 5 0.15425125837044362 1103 6 0.08171466227208413 1103 7 0.11571542099959942 1103 8 0.19970687419333188 1103 9 0.14403270339689556 1103 10-11 0.16978661095206604 1103 12-13 0.16601930192192782 1103 14-15 0.16607002656657954 1103 16-17 0.31357020554299453 1103 18-19 0.21296730474232817 1103 20-21 0.27600899376332677 1103 22-23 0.2498196953733185 1103 24-25 0.31290115449468203 1103 26-27 0.1746605336685647 1103 28-29 0.1870583535822945 1103 30-31 0.20407379930034608 1103 32-33 0.2433263309928151 1103 34-35 0.3364215727614024 1103 36-37 0.3500008096884031 1103 38-39 0.43095858194654113 1103 40-41 0.4044250065037147 1103 42-43 0.4555100800549141 1103 44-45 0.6167166456878661 1103 46-47 0.5656044835623675 1103 48-49 0.5777484868055822 1103 50-51 0.4539925154661404 1103 52-53 0.5647775700759325 1103 54-55 0.5715368432860544 1103 56-57 0.4975952679069735 1103 58-59 0.5415288751153753 1103 60-61 0.4941551438590821 1103 62-63 0.4383673854320307 1103 64-65 0.29868935741514235 1103 66-67 0.40869712115244283 1103 68-69 0.5080762834449857 1103 70-71 0.41673035105998935 1103 72-73 0.534568380505462 1103 74-75 0.49628202694876933 1103 76-77 0.3791058633884141 1103 78-79 0.48972438717889943 1103 80-81 0.6335779395995544 1103 82-83 0.6854853501958367 1103 84-85 0.5097456060069554 1103 86-87 0.2379652282755309 1103 88-89 0.513489366716378 1103 90-91 0.696520927213836 1103 92-93 0.6458222318494222 1103 94-95 0.5521082506000674 1103 96-97 0.9369818853755056 1103 98-99 1.0543412905829186 1103 100-101 1.0317741963252267 1104 1 0.18197982097119336 1104 2 0.01914827429356336 1104 3 0.136859850531458 1104 4 0.05338116828995254 1104 5 0.08845377987345415 1104 6 0.13019977823633866 1104 7 0.1384905200825628 1104 8 0.12182806567712845 1104 9 0.21041267595005309 1104 10-11 0.18189229082079095 1104 12-13 0.12176364067703815 1104 14-15 0.11981501439736064 1104 16-17 0.17411690532038193 1104 18-19 0.29177197823938883 1104 20-21 0.2350302896090568 1104 22-23 0.21199391626374364 1104 24-25 0.1677252591716396 1104 26-27 0.1631078617101096 1104 28-29 0.22099919029638926 1104 30-31 0.3020908845244392 1104 32-33 0.2126835672544587 1104 34-35 -0.01659208255811251 1104 36-37 -0.0568625378147658 1104 38-39 0.081034694249567 1104 40-41 0.1804465302488154 1104 42-43 0.17641153431162593 1104 44-45 0.21678178153529615 1104 46-47 0.14810291508964468 1104 48-49 0.4191082661046295 1104 50-51 0.326016554855066 1104 52-53 0.33700013683161245 1104 54-55 0.31079275754110824 1104 56-57 0.260087596753209 1104 58-59 0.31911259389288205 1104 60-61 0.3779087720817742 1104 62-63 0.3938819224824144 1104 64-65 0.1870534774553363 1104 66-67 0.3210218735734429 1104 68-69 0.19340831456865715 1104 70-71 0.28961443474281623 1104 72-73 0.3177769145189657 1104 74-75 0.24156624105547309 1104 76-77 0.1337836026418593 1104 78-79 0.038948749355519396 1104 80-81 0.21408577092967107 1104 82-83 0.15589514674904237 1104 84-85 0.31089617444277806 1104 86-87 0.159637469409045 1104 88-89 0.32343822061842076 1104 90-91 0.3537517101413954 1104 92-93 0.3896960570167387 1104 94-95 0.26947079665076856 1104 96-97 0.5322814893480299 1104 98-99 0.6408230150696994 1104 100-101 0.9211417964915647 1105 1 0.14014783685846766 1105 2 -0.027406487572307014 1105 3 -0.038511136519289835 1105 4 0.08090293511942548 1105 5 0.02953317191908411 1105 6 0.01642886026071011 1105 7 0.04990308288608247 1105 8 0.0972545928556201 1105 9 0.20696545423992774 1105 10-11 0.0861861494361591 1105 12-13 0.1603355593084146 1105 14-15 0.24633915918440863 1105 16-17 0.21698291592901597 1105 18-19 0.1839391866945519 1105 20-21 0.10244480331790129 1105 22-23 0.2635895281269285 1105 24-25 0.24406516475342244 1105 26-27 0.08128791907046207 1105 28-29 0.10083392341207542 1105 30-31 0.26753372369843476 1105 32-33 0.19262821381622075 1105 34-35 0.2304924107782398 1105 36-37 0.09703612245467497 1105 38-39 0.3025084212021696 1105 40-41 0.19803921575400807 1105 42-43 -0.004234131341469549 1105 44-45 0.26758326291651713 1105 46-47 0.2463772039776515 1105 48-49 0.2009018388516992 1105 50-51 0.34325079651063106 1105 52-53 0.41868356277071683 1105 54-55 0.3506859711442374 1105 56-57 0.38646449019317686 1105 58-59 0.2957304334752564 1105 60-61 0.46755147170535594 1105 62-63 0.31182278391446516 1105 64-65 0.33735066381113654 1105 66-67 0.35503973509183595 1105 68-69 0.2860095761229857 1105 70-71 0.08217509132980183 1105 72-73 0.23237766734439447 1105 74-75 0.31754256882624077 1105 76-77 0.1566364983127606 1105 78-79 0.0689621209913156 1105 80-81 0.3007512396720138 1105 82-83 0.4204621787826248 1105 84-85 0.41682473303644585 1105 86-87 0.3789383638851369 1105 88-89 0.5951449594094669 1105 90-91 0.6424838317042685 1105 92-93 0.6207446219578543 1105 94-95 0.5669885263196974 1105 96-97 0.6009878944315865 1105 98-99 0.7462764488731004 1105 100-101 0.8979409582556883 1106 1 0.36544104125045607 1106 2 0.21095792760885956 1106 3 0.1976460621863083 1106 4 0.21601451178278808 1106 5 0.27825654035588343 1106 6 0.26136255024032096 1106 7 0.18303938785605567 1106 8 0.23975847418967078 1106 9 0.38446306932382157 1106 10-11 0.3843623900601685 1106 12-13 0.39030881083756697 1106 14-15 0.5455254819243507 1106 16-17 0.5767012152500186 1106 18-19 0.5318488778712975 1106 20-21 0.35112327921621755 1106 22-23 0.44095620664023016 1106 24-25 0.5256367777049178 1106 26-27 0.5181222468982227 1106 28-29 0.483286552431359 1106 30-31 0.5923967079299288 1106 32-33 0.63518072873363 1106 34-35 0.6626466914688365 1106 36-37 0.5808467507631647 1106 38-39 0.7315389294231096 1106 40-41 0.6368076637815392 1106 42-43 0.5448689531500293 1106 44-45 0.6313250559993264 1106 46-47 0.19556633250124378 1106 48-49 0.22062649794897737 1106 50-51 0.33896355908522224 1106 52-53 0.28663590185902876 1106 54-55 0.5859397075128001 1106 56-57 0.6004485159315607 1106 58-59 0.5705700361762922 1106 60-61 0.3776803507012616 1106 62-63 0.5144639615953892 1106 64-65 0.5415219288454622 1106 66-67 0.8078240077391854 1106 68-69 0.7736622575169037 1106 70-71 0.6136428902860516 1106 72-73 0.7989720071435045 1106 74-75 0.6713892982177541 1106 76-77 0.52571115086322 1106 78-79 0.6559376732630682 1106 80-81 0.6601833490601656 1106 82-83 0.8256229501860766 1106 84-85 0.6197801584867726 1106 86-87 0.5614789074589552 1106 88-89 0.830344450627365 1106 90-91 0.9664639293455366 1106 92-93 1.0661197552325774 1106 94-95 0.8638872001192901 1106 96-97 1.5544792245600867 1106 98-99 1.5761767500272157 1106 100-101 1.3447489117170903 1107 1 0.15185700261307744 1107 2 0.10835318702788754 1107 3 0.054636191837211356 1107 4 0.07057573842570264 1107 5 0.008427923377013258 1107 6 0.06672207946452602 1107 7 0.07371999648851357 1107 8 0.13255851170168853 1107 9 0.23549347750144278 1107 10-11 0.1543551221071553 1107 12-13 0.16209977513081952 1107 14-15 0.25039032144325546 1107 16-17 0.19518994992284178 1107 18-19 0.331138494076491 1107 20-21 0.17724102005507802 1107 22-23 0.2728859653910831 1107 24-25 0.13695602840240895 1107 26-27 0.05431665291890653 1107 28-29 0.17749563502813004 1107 30-31 0.26737853804738165 1107 32-33 0.24228925568367288 1107 34-35 0.35373758777155473 1107 36-37 0.23104955009731754 1107 38-39 0.3516875707847973 1107 40-41 0.17068570284092743 1107 42-43 0.45844126928253814 1107 44-45 0.4146486205786033 1107 46-47 0.4056336196791719 1107 48-49 0.3703041290651967 1107 50-51 0.4163203686882966 1107 52-53 0.3792108672776351 1107 54-55 0.32611273168460997 1107 56-57 0.3104431235793257 1107 58-59 0.3988798854248756 1107 60-61 0.5205073499744657 1107 62-63 0.44805128253413073 1107 64-65 0.34038250137259496 1107 66-67 0.09632891946873201 1107 68-69 0.4098918310521711 1107 70-71 0.4713856047492726 1107 72-73 0.440530631390331 1107 74-75 0.35009888488223595 1107 76-77 0.31374481789719155 1107 78-79 0.5360398935054889 1107 80-81 0.5534522867667739 1107 82-83 0.4807820245770955 1107 84-85 0.4392090380497571 1107 86-87 0.32427871052087553 1107 88-89 0.43222942940963094 1107 90-91 0.56383574375484 1107 92-93 0.7326824823561076 1107 94-95 0.5562839834639526 1107 96-97 0.5196118060901078 1107 98-99 0.36609774034442566 1107 100-101 0.3271534319149687 1108 1 0.0372860900618619 1108 2 -0.13322139592513338 1108 3 -0.009865779304497835 1108 4 -0.002513807726202799 1108 5 -0.04763018160813459 1108 6 -0.09593692272994758 1108 7 -0.10978455752854188 1108 8 0.023258218457947066 1108 9 -0.03933999500938512 1108 10-11 0.009613118339650839 1108 12-13 -0.010943162744233348 1108 14-15 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0.0 4 4.0 5 16.0 6 38.0 7 79.0 8 246.0 9 484.0 10 885.0 11 1440.0 12 2560.0 13 3591.0 14 4624.0 15 6641.0 16 9180.0 17 13697.0 18 12062.0 19 6320.0 20 4982.0 21 4929.0 22 4972.0 23 5439.0 24 6272.0 25 7155.0 26 8760.0 27 11000.0 28 13947.0 29 17414.0 30 22068.0 31 28243.0 32 37948.0 33 50667.0 34 71448.0 35 104773.0 36 168607.0 37 275556.0 38 236888.0 39 21974.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.25709210267611 20.90037786197606 15.535956988471819 21.306573046876007 2 15.045931257205597 21.83917321161686 40.861435342130136 22.253460189047406 3 18.824005102719703 26.68652011513741 30.935324057898356 23.55415072424453 4 12.474697616124852 18.52280433681572 39.07074366260054 29.931754384458888 5 12.811168856588049 38.55660916728046 34.777084717593716 13.855137258537775 6 29.59054206948887 38.71969068748895 17.99819041193023 13.69157683109195 7 26.616476809367246 34.30119064992145 22.096127597840177 16.986204942871122 8 25.455357066211125 35.62670077516718 20.416512863654702 18.501429294966993 9 26.51741336240568 17.27669948751406 21.42113983054485 34.78474731953541 10-11 23.815473602387833 27.95387440966292 28.287162961147526 19.94348902680172 12-13 25.32348186930586 25.562042504826582 28.767631296339697 20.346844329527865 14-15 22.380183876866024 26.617764462490662 27.55809461675151 23.443957043891803 16-17 20.769673193637278 29.961155797443578 28.151445176022182 21.117725832896962 18-19 20.542230730271008 28.125305817616812 31.469383900902216 19.863079551209964 20-21 21.38478998211828 27.250774116065475 31.644757343479185 19.719678558337062 22-23 21.128542119133666 27.123812139993646 31.400794911194858 20.34685082967783 24-25 21.063265211516438 27.29479754891945 31.6219179070011 20.020019332563006 26-27 20.83091894434758 27.515192408560115 31.53483888192114 20.119049765171162 28-29 20.522193144245886 27.510665302858545 31.729098946625626 20.23804260626994 30-31 20.841988769075765 27.431453027116575 31.454830108767652 20.27172809504001 32-33 20.83795638358648 27.617347501578454 31.226567305244934 20.31812880959013 34-35 21.133793836389927 27.506308559940013 31.29110114255865 20.06879646111141 36-37 21.26824029545029 27.192913176279504 31.201485836139092 20.337360692131114 38-39 20.798524833556602 27.826726317736806 31.273288233049513 20.101460615657075 40-41 21.052109722209106 27.57855175131281 31.28931236044568 20.080026166032404 42-43 21.006293303482135 27.83728750121256 30.805381456945685 20.35103773835962 44-45 21.25513023786448 27.72805039372242 30.506083318239142 20.51073605017396 46-47 21.431511573655783 28.16641822083022 29.856593886987692 20.545476318526305 48-49 21.42408609774931 28.333083671378485 29.697353912345903 20.545476318526305 50-51 21.400779546204703 28.40330377879464 29.484161202360532 20.711755472640125 52-53 21.642247493878216 28.508774377144768 29.293693221935836 20.555284907041177 54-55 21.348985464447473 28.505676179618632 29.261126582582747 20.884211773351144 56-57 21.432281115010333 28.762026742117808 29.064412174737146 20.741279968134712 58-59 21.5349082365222 28.3505322788195 29.161548188380603 20.953011296277698 60-61 21.452197061199403 28.66382177995136 29.324738582037725 20.559242576811513 62-63 21.337442377522727 28.38403825188212 29.70594294838228 20.572576422212872 64-65 21.23945463481006 28.478269733862987 29.30511650066636 20.97715913066059 66-67 21.265952962849457 28.687734406721898 28.949385746011064 21.09692688441758 68-69 21.358173592809745 28.491771896541362 29.106068279952957 21.043986230695932 70-71 21.440500640608803 28.532565890424777 29.048915901047938 20.978017567918485 72-73 21.422127031272804 28.414512709136332 29.149204659587436 21.014155600003434 74-75 21.440233906883634 28.790041788707775 28.667371157207267 21.102353147201324 76-77 21.456102820489928 28.612701132453832 28.89109317162616 21.04010287543008 78-79 21.55275981377046 28.62238299812045 28.580920442971173 21.243936745137916 80-81 21.49266301011889 28.887085129342722 28.467546493070788 21.1527053674676 82-83 21.58134515936296 28.831312538201097 28.424621079451416 21.162721222984526 84-85 21.434887304537437 28.718005949187248 28.52755469517927 21.31955205109605 86-87 21.260496158222598 28.795753763980393 28.570839920147 21.37291015765001 88-89 21.40917977761992 28.627797724692517 28.427908893271724 21.53511360441584 90-91 21.08879889604969 28.679830802016472 28.750179735300875 21.481190566632975 92-93 21.392553931204986 28.53503704148818 28.601737473281197 21.470671554025632 94-95 21.197434131036154 28.510804505937166 28.42890959153409 21.862851771492586 96-97 21.45768815577403 28.45000718950461 28.3683687550568 21.72393589966456 98-99 21.078735621823153 28.546459571129212 28.36068824670157 22.014116560346064 100-101 20.718672055977407 28.901573828106052 28.189966765344792 22.189787350571745 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 351.0 1 240.0 2 147.0 3 190.0 4 202.5 5 198.5 6 311.5 7 698.5 8 1207.5 9 1497.5 10 1443.0 11 1350.5 12 1450.5 13 1583.0 14 1760.0 15 1925.5 16 2043.5 17 2167.0 18 2311.5 19 2445.5 20 2715.0 21 3121.5 22 3633.5 23 4391.5 24 5251.0 25 6537.5 26 8479.5 27 10689.5 28 12536.0 29 14742.0 30 17709.0 31 20866.0 32 24166.0 33 27687.0 34 31657.0 35 35319.5 36 39847.5 37 43439.0 38 44821.0 39 45228.0 40 44141.0 41 43372.0 42 42382.5 43 42334.0 44 43514.0 45 43276.5 46 41870.5 47 41288.5 48 43243.5 49 41821.0 50 37497.0 51 34539.0 52 31473.0 53 29667.0 54 27813.0 55 24681.0 56 21784.5 57 19278.5 58 17289.5 59 15298.5 60 12746.0 61 10140.0 62 7859.0 63 5988.0 64 4336.0 65 3186.5 66 2354.5 67 1778.5 68 1238.5 69 736.5 70 514.0 71 369.0 72 254.5 73 192.0 74 151.5 75 110.0 76 71.0 77 39.5 78 26.0 79 24.0 80 16.0 81 10.5 82 6.5 83 4.0 84 3.0 85 1.5 86 1.5 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01708286477066898 2 0.001459340206539561 3 0.003519585204007177 4 0.0 5 0.0024036191637122183 6 3.433741662446026E-4 7 0.0 8 0.0 9 0.0 10-11 3.004523954640273E-4 12-13 6.009047909280546E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.002832836871517971 22-23 0.0 24-25 0.003090367496201423 26-27 0.0016310272896618624 28-29 0.0029616021838596972 30-31 0.015408915710226541 32-33 3.8629593702517787E-4 34-35 0.002103166768248191 36-37 0.003777115828690628 38-39 0.002103166768248191 40-41 0.003519585204007177 42-43 0.0018027143727841635 44-45 2.5753062468345195E-4 46-47 1.2876531234172597E-4 48-49 1.2876531234172597E-4 50-51 1.2876531234172597E-4 52-53 0.001073044269514383 54-55 0.003605428745568327 56-57 5.150612493669039E-4 58-59 0.0011159660402949583 60-61 0.0011159660402949583 62-63 0.0 64-65 2.1460885390287663E-4 66-67 8.584354156115064E-5 68-69 0.0 70-71 2.1460885390287663E-4 72-73 0.0 74-75 1.716870831223013E-4 76-77 5.150612493669039E-4 78-79 3.004523954640273E-4 80-81 0.005064768952107888 82-83 0.001888557914345314 84-85 0.003862959370251779 86-87 0.0016739490604424376 88-89 0.001073044269514383 90-91 2.1460885390287663E-4 92-93 0.0 94-95 2.1460885390287663E-4 96-97 0.0015022619773201365 98-99 0.003176211037762574 100-101 9.872007279532326E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1164910.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.06038306175877 #Duplication Level Percentage of deduplicated Percentage of total 1 80.08190136761043 45.69503968349835 2 11.81846494926552 13.487322744141201 3 3.5473465677035425 6.07238862017938 4 1.4584399910245789 3.3287657824180203 5 0.754719270568999 2.1532285341379125 6 0.4502060402362561 1.5413357467559015 7 0.2870097975952649 1.1463822295284567 8 0.22402142137214073 1.0226198494027219 9 0.1641307356864219 0.8428826385427962 >10 1.0396944680539975 11.693362422108317 >50 0.10361879932054092 4.12311172907789 >100 0.0680278266407822 6.778297618939698 >500 0.0016629008836374633 0.5572381988500241 >1k 4.535184228102172E-4 0.555426743404846 >5k 3.0234561520681147E-4 1.0025974590145248 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6604 0.5669107484698389 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5019 0.4308487350954151 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3462 0.29719034088470353 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1681 0.14430299336429425 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1296 0.11125322986325124 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.716870831223013E-4 0.0 18-19 0.0 0.0 0.0 2.146088539028766E-4 0.0 20-21 0.0 0.0 0.0 4.292177078057532E-4 0.0 22-23 0.0 0.0 0.0 8.155136448309312E-4 0.0 24-25 0.0 0.0 0.0 0.001073044269514383 0.0 26-27 0.0 0.0 0.0 0.0018027143727841637 0.0 28-29 0.0 0.0 0.0 0.0032620545793237248 0.0 30-31 0.0 0.0 0.0 0.006051969680061121 0.0 32-33 0.0 0.0 0.0 0.00935694603016542 0.0 34-35 0.0 0.0 0.0 0.013906653732906405 0.0 36-37 0.0 0.0 0.0 0.01991570164218695 0.0 38-39 0.0 0.0 0.0 0.027469933299568206 0.0 40-41 0.0 0.0 0.0 0.04335098848838108 0.0 42-43 0.0 0.0 0.0 0.05863113888626589 0.0 44-45 0.0 0.0 0.0 0.07524186417834855 0.0 46-47 0.0 0.0 0.0 0.11078109038466491 0.0 48-49 0.0 0.0 0.0 0.13138354035934108 0.0 50-51 0.0 0.0 0.0 0.1539174700191431 0.0 52-53 0.0 0.0 0.0 0.18031435904919693 0.0 54-55 0.0 0.0 0.0 0.2180855173361032 0.0 56-57 0.0 0.0 0.0 0.3153462499248869 0.0 58-59 0.0 0.0 0.0 0.4037221759620915 0.0 60-61 0.0 0.0 0.0 0.467546849112807 0.0 62-63 0.0 0.0 0.0 0.5515447545303929 0.0 64-65 0.0 0.0 0.0 0.6329244319303637 0.0 66-67 0.0 0.0 0.0 0.7564532882368595 0.0 68-69 0.0 0.0 0.0 0.9190409559536787 0.0 70-71 0.0 0.0 0.0 1.0009356946030166 0.0 72-73 0.0 0.0 0.0 1.0962649475066741 0.0 74-75 0.0 0.0 0.0 1.276407619472749 0.0 76-77 0.0 0.0 0.0 1.4792129864109673 0.0 78-79 0.0 0.0 0.0 1.6620597299362183 0.0 80-81 0.0 0.0 0.0 1.7950313758144407 0.0 82-83 0.0 0.0 0.0 2.010842039299173 0.0 84-85 0.0 0.0 0.0 2.359452661579006 0.0 86-87 0.0 0.0 0.0 2.7044149333424903 0.0 88-89 0.0 0.0 0.0 3.096762840047729 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5600 0.0 30.875347 1 GTATCAA 8200 0.0 28.616179 1 ATCAACG 9770 0.0 23.919409 3 TCAACGC 9835 0.0 23.760303 4 CGCGGAA 90 1.9542313E-7 23.74823 82-83 CAACGCA 9885 0.0 23.640121 5 GTACATG 4695 0.0 23.472044 1 TATCAAC 9970 0.0 23.43958 2 AACGCAG 10045 0.0 23.263573 6 AGGACCG 165 2.431505E-6 23.028585 5 CGATCGT 95 3.2814387E-7 22.506052 24-25 GAGTACT 5935 0.0 22.407764 12-13 ACATGGG 4715 0.0 21.65891 3 CTCCCGC 220 5.6439603E-8 21.589298 7 TACATGG 5080 0.0 21.318224 2 GTACTTT 6260 0.0 21.28373 14-15 CTGGTCG 380 0.0 21.248415 9 ACGCAGA 11200 0.0 20.694885 7 GGACCGA 115 0.0037249161 20.650635 6 CGCAGAG 11195 0.0 20.619276 8 >>END_MODULE