FastQCFastQC Report
Thu 26 May 2016
SRR936918_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936918_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888552
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43520.48978562875329745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29840.33582727853856614No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21460.24151653476667656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45950.036.4029731
GCGTATC702.0477959E-433.9431151
GGTATCA27450.032.892051
ATCAACG54300.030.2800063
CAACGCA54550.030.1463285
TCAACGC55000.029.8946254
AACGCAG55600.029.7446336
TATCAAC56700.029.3335532
CGTATCA1004.937413E-528.5122152
TGCACCG1257.354214E-626.6158985
ACGCAGA61950.026.312197
GTACATG34500.026.1706331
CGCAGAG62600.026.1148938
GTATAGG2900.024.5794941
ACATGGG33450.024.434983
GCAGAGT64650.024.4047139
TACATGG36600.024.0198542
GAGTACT34500.022.72648812-13
AGAGTAC53450.022.04873710-11
AGTACTT37500.021.4152412-13
Produced by FastQC (version 0.11.5)