##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936918_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 888552 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.040555870674986 33.0 31.0 34.0 30.0 34.0 2 32.16861703085469 33.0 31.0 34.0 30.0 34.0 3 32.18826360190512 34.0 31.0 34.0 30.0 34.0 4 35.749428283319375 37.0 35.0 37.0 35.0 37.0 5 35.755369410006395 37.0 35.0 37.0 35.0 37.0 6 35.82117985216397 37.0 35.0 37.0 35.0 37.0 7 35.77305098632382 37.0 35.0 37.0 35.0 37.0 8 35.72371116153022 37.0 35.0 37.0 35.0 37.0 9 37.416340292971036 39.0 37.0 39.0 35.0 39.0 10-11 37.265767225778575 39.0 37.0 39.0 34.0 39.0 12-13 37.221723095553216 39.0 37.0 39.0 34.0 39.0 14-15 38.449352992284076 40.0 38.0 41.0 34.0 41.0 16-17 38.387023494404374 40.0 38.0 41.0 34.0 41.0 18-19 38.390340689121174 40.0 38.0 41.0 34.0 41.0 20-21 38.32866956576542 40.0 38.0 41.0 34.0 41.0 22-23 38.22141585410871 40.0 38.0 41.0 34.0 41.0 24-25 38.07857896892922 40.0 38.0 41.0 33.5 41.0 26-27 38.16348283499447 40.0 38.0 41.0 34.0 41.0 28-29 38.114350651396876 40.0 38.0 41.0 34.0 41.0 30-31 37.969529076520004 40.0 38.0 41.0 33.5 41.0 32-33 37.76585219548209 40.0 37.0 41.0 33.0 41.0 34-35 37.609200699565136 40.0 37.0 41.0 33.0 41.0 36-37 37.435528815421044 40.0 37.0 41.0 32.5 41.0 38-39 37.283662633137965 40.0 37.0 41.0 32.0 41.0 40-41 37.123173995444276 40.0 37.0 41.0 31.5 41.0 42-43 36.91977115576803 40.0 36.0 41.0 31.0 41.0 44-45 36.651634907129804 39.0 36.0 41.0 30.5 41.0 46-47 36.2920391828503 39.0 36.0 41.0 30.0 41.0 48-49 35.966949036184715 39.0 36.0 41.0 29.0 41.0 50-51 35.34473953128236 38.5 35.0 40.0 28.5 40.5 52-53 35.59207395853028 39.0 35.5 40.0 28.5 40.5 54-55 36.00160204467493 39.0 36.0 41.0 29.0 41.0 56-57 35.91516872394638 39.0 36.0 41.0 28.0 41.0 58-59 35.70786121690121 39.0 35.0 41.0 28.0 41.0 60-61 35.46408989006834 39.0 35.0 41.0 28.0 41.0 62-63 35.174335885800716 38.5 35.0 40.5 27.0 41.0 64-65 34.88832617562056 38.0 35.0 40.0 26.0 41.0 66-67 34.52688081282806 38.0 34.0 40.0 26.0 41.0 68-69 34.165873803671595 37.0 34.0 40.0 26.0 41.0 70-71 33.75316132314147 36.5 34.0 39.0 25.0 41.0 72-73 33.35487568538476 36.0 34.0 39.0 24.0 41.0 74-75 32.9203473741548 35.5 33.0 38.5 22.5 40.0 76-77 32.48190876842323 35.0 33.0 37.5 22.0 39.5 78-79 32.02492482150735 35.0 32.0 37.0 20.0 39.0 80-81 31.58253596863211 35.0 32.0 36.5 19.0 39.0 82-83 31.16417778588085 35.0 31.5 36.0 18.0 37.5 84-85 30.79105612277053 35.0 31.0 36.0 15.0 37.0 86-87 30.4571257506595 34.5 31.0 35.0 9.5 37.0 88-89 30.100510718562333 34.0 31.0 35.0 7.0 36.0 90-91 29.750169376693766 34.0 30.5 35.0 2.0 36.0 92-93 29.394135627402786 34.0 30.0 35.0 2.0 36.0 94-95 29.000220583601184 34.0 30.0 35.0 2.0 35.0 96-97 28.55632084560048 34.0 29.0 35.0 2.0 35.0 98-99 28.00508073809974 34.0 29.0 35.0 2.0 35.0 100-101 25.878814070532734 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16831256352157098 1101 2 0.2239956534379175 1101 3 0.1608119998021209 1101 4 0.14495966289763373 1101 5 0.17782954430924036 1101 6 0.005091002927173349 1101 7 0.07955561596167371 1101 8 0.16545792000967197 1101 9 -0.011585044876255779 1101 10-11 0.14435071477467432 1101 12-13 0.15872202449527606 1101 14-15 0.28847134758735393 1101 16-17 0.3977883737082877 1101 18-19 0.28892628763129125 1101 20-21 0.30015931332731327 1101 22-23 0.3619133303317099 1101 24-25 0.3948887175109448 1101 26-27 0.5473719688808174 1101 28-29 0.34360658809145406 1101 30-31 0.2641255126686204 1101 32-33 0.34908449518334095 1101 34-35 0.41327679339434553 1101 36-37 0.4058229207331365 1101 38-39 0.5809896964459753 1101 40-41 0.6072342444973202 1101 42-43 0.7233752436161112 1101 44-45 0.7254488871696481 1101 46-47 0.6691487809081877 1101 48-49 0.7426202840496856 1101 50-51 0.6265466075284536 1101 52-53 0.6702797705968777 1101 54-55 0.7850096319896167 1101 56-57 0.8374340317320517 1101 58-59 0.8832256461195271 1101 60-61 0.9341763823408584 1101 62-63 0.8776046588630564 1101 64-65 0.9485011962865499 1101 66-67 0.815615459526974 1101 68-69 0.6230449502656299 1101 70-71 0.7595534954769363 1101 72-73 0.8073319826459908 1101 74-75 0.7142790872692544 1101 76-77 0.7933724686760328 1101 78-79 0.8859961003721608 1101 80-81 0.6961484253732273 1101 82-83 0.8458449615648895 1101 84-85 0.8508313906916705 1101 86-87 0.8348341820973069 1101 88-89 0.7830527851498026 1101 90-91 0.7779319251634469 1101 92-93 0.6572336255219824 1101 94-95 0.7198586235808193 1101 96-97 0.6771819674865185 1101 98-99 0.7362533890985503 1101 100-101 0.7103131004986096 1102 1 0.2898132760903387 1102 2 0.20299219154745174 1102 3 0.2490025172582122 1102 4 0.26761581272719326 1102 5 0.11553725405278925 1102 6 0.06453117588905855 1102 7 0.12422417200406954 1102 8 0.15656554810625067 1102 9 0.08406128521036038 1102 10-11 0.2159030830526092 1102 12-13 0.2420993867675989 1102 14-15 0.1913567721294882 1102 16-17 0.20313643444537632 1102 18-19 0.011815146062218673 1102 20-21 0.12593366998898858 1102 22-23 0.11063391200004702 1102 24-25 0.23049423885042586 1102 26-27 0.15678646136532137 1102 28-29 0.20858217505799814 1102 30-31 0.21332643348137736 1102 32-33 0.238480484460176 1102 34-35 0.29445687212324856 1102 36-37 0.03346852572391157 1102 38-39 0.3322254542816552 1102 40-41 0.22838640998423188 1102 42-43 0.13382590836097563 1102 44-45 0.14833450330214504 1102 46-47 0.2679429038001402 1102 48-49 0.5846610308983955 1102 50-51 0.5748127884398642 1102 52-53 0.586318057720085 1102 54-55 0.6406477679038218 1102 56-57 0.733592976570236 1102 58-59 0.5838990493980774 1102 60-61 0.40617729313196094 1102 62-63 0.5809592218822885 1102 64-65 0.5984794213050009 1102 66-67 0.4664051802619156 1102 68-69 0.31825176469268257 1102 70-71 0.2876918561413717 1102 72-73 0.4715737416813113 1102 74-75 0.28477162924596655 1102 76-77 0.27849179262732804 1102 78-79 0.2632054688461096 1102 80-81 0.3418298680781291 1102 82-83 0.14790964247113791 1102 84-85 0.24372629895673015 1102 86-87 0.06515858879354042 1102 88-89 0.2542681754562146 1102 90-91 0.1901647487190452 1102 92-93 0.24711069082924197 1102 94-95 0.252022224064806 1102 96-97 0.3467317006600972 1102 98-99 0.14787570259638372 1102 100-101 0.1451721931561316 1103 1 0.26198440119686595 1103 2 0.21194284732263924 1103 3 0.3102320177882376 1103 4 0.14353166357211222 1103 5 0.2703667109337502 1103 6 0.234315632666096 1103 7 0.04611443618481559 1103 8 0.2024892973527841 1103 9 0.19193032763309503 1103 10-11 0.3602236386428146 1103 12-13 0.11859137924333396 1103 14-15 0.20178318189024935 1103 16-17 0.34877620497602635 1103 18-19 0.3201288632994448 1103 20-21 0.36879198201742014 1103 22-23 0.4067785047127259 1103 24-25 0.29763195163960177 1103 26-27 0.3493288373292387 1103 28-29 0.33299758941230806 1103 30-31 0.344046928794306 1103 32-33 0.3312828061745492 1103 34-35 0.18366749963047369 1103 36-37 0.137756006757364 1103 38-39 0.2719182999926133 1103 40-41 0.2957521111752257 1103 42-43 0.2977041014976791 1103 44-45 0.2774883090846103 1103 46-47 0.34193845575291704 1103 48-49 0.46329975177302885 1103 50-51 0.16183031042028517 1103 52-53 0.2144722220484141 1103 54-55 0.3921780832163293 1103 56-57 0.41717365458566036 1103 58-59 0.4770134099393246 1103 60-61 0.6611228876945461 1103 62-63 0.674796260189531 1103 64-65 0.5226333310138074 1103 66-67 0.6339539217139745 1103 68-69 0.5619992964421741 1103 70-71 0.44046941740595 1103 72-73 0.46619295558249974 1103 74-75 0.31438219127959854 1103 76-77 0.40357168599890514 1103 78-79 0.4222484891533185 1103 80-81 0.39115200854496734 1103 82-83 0.3220888842245877 1103 84-85 0.2200596738606002 1103 86-87 0.237323608227765 1103 88-89 0.29010847185412914 1103 90-91 0.36175882283930605 1103 92-93 0.40417651257478937 1103 94-95 0.3753840851776502 1103 96-97 0.419715269906213 1103 98-99 0.525809672998168 1103 100-101 0.5748270537909121 1104 1 0.34642597287167476 1104 2 0.32533908002650236 1104 3 0.4232967312889855 1104 4 0.3909979521759439 1104 5 0.29086971543812723 1104 6 0.30914255226345944 1104 7 0.3168632529529063 1104 8 0.36713649425971084 1104 9 0.26947308587286045 1104 10-11 0.49788237503683774 1104 12-13 0.5268792998025233 1104 14-15 0.6471223909104324 1104 16-17 0.6404642983775446 1104 18-19 0.5995075951224322 1104 20-21 0.6331374785939019 1104 22-23 0.35897129294992425 1104 24-25 0.6511784333697435 1104 26-27 0.4038613654934906 1104 28-29 0.4149786577016883 1104 30-31 0.5653669466022961 1104 32-33 0.44409516035370444 1104 34-35 0.5394590270233621 1104 36-37 0.5110323449759875 1104 38-39 0.6627139698875766 1104 40-41 0.5924174565265758 1104 42-43 0.6989253658654988 1104 44-45 0.7616203239595265 1104 46-47 0.6091774543149384 1104 48-49 0.652451991104904 1104 50-51 0.5412120776973808 1104 52-53 0.5351792566835343 1104 54-55 0.7773343555358423 1104 56-57 0.7705171775217892 1104 58-59 0.6902582270502435 1104 60-61 0.8150257674477075 1104 62-63 0.9530637777356432 1104 64-65 1.029094384352092 1104 66-67 0.761737611683408 1104 68-69 0.7956310565770153 1104 70-71 0.608914800658809 1104 72-73 0.6853563130090095 1104 74-75 0.7724247757247369 1104 76-77 0.7051098279676964 1104 78-79 0.8862822267377197 1104 80-81 0.9513414923035022 1104 82-83 0.9371689011755109 1104 84-85 0.9455820100413099 1104 86-87 1.1073779011931286 1104 88-89 1.0287148055969482 1104 90-91 1.0288546193900778 1104 92-93 0.7995197093483988 1104 94-95 0.8554197249799529 1104 96-97 0.9417830212418146 1104 98-99 1.1331364854618577 1104 100-101 0.8949246168569367 1105 1 0.26402629161306734 1105 2 0.2080912594045472 1105 3 0.26750781793909084 1105 4 0.23386526146464348 1105 5 0.3423268131549264 1105 6 0.4288904804038296 1105 7 0.43335803891822167 1105 8 0.2997999405130827 1105 9 0.3777810543265545 1105 10-11 0.46588935683659827 1105 12-13 0.43042188967371686 1105 14-15 0.3385307361248877 1105 16-17 0.31527991443373793 1105 18-19 0.356436447142201 1105 20-21 0.46403575643285677 1105 22-23 0.37313938034738925 1105 24-25 0.38944736249440837 1105 26-27 0.4561372261074226 1105 28-29 0.3758589941653625 1105 30-31 0.10750321179384059 1105 32-33 0.1038906562651718 1105 34-35 0.25525538957383986 1105 36-37 -0.08499580294014208 1105 38-39 0.06412323205156412 1105 40-41 0.23450771194976738 1105 42-43 0.42321527771662915 1105 44-45 0.5801373487910055 1105 46-47 0.4247736297428091 1105 48-49 0.37454365846807747 1105 50-51 0.2863532595235938 1105 52-53 0.36119984990931187 1105 54-55 0.5375857295400763 1105 56-57 0.7610583968374556 1105 58-59 0.5105491029155615 1105 60-61 0.5831799480478139 1105 62-63 0.6031461003282317 1105 64-65 0.49791850612947997 1105 66-67 0.5334756043104818 1105 68-69 0.6814878809941618 1105 70-71 0.42441116653628086 1105 72-73 0.5040741741309134 1105 74-75 0.5374284638093414 1105 76-77 0.5001403672557316 1105 78-79 0.5235398335643637 1105 80-81 0.6948244561115793 1105 82-83 0.6566415821655234 1105 84-85 0.5532424336070711 1105 86-87 0.5172198531758063 1105 88-89 0.5561867757482126 1105 90-91 0.3581190230036704 1105 92-93 0.36494892693078285 1105 94-95 0.39983822074453457 1105 96-97 0.28851571909396156 1105 98-99 0.2837953502134276 1105 100-101 0.48712145751950686 1106 1 0.17110796441525267 1106 2 0.1674026174375811 1106 3 0.09938431663881175 1106 4 0.13814373253762824 1106 5 -0.05686030056050129 1106 6 0.12691620737874132 1106 7 0.11041041319631262 1106 8 0.1428628605711708 1106 9 0.0349619368284948 1106 10-11 0.21367191450488576 1106 12-13 0.0848270114272438 1106 14-15 0.06241536579829443 1106 16-17 0.20940838888407143 1106 18-19 0.04857713916150885 1106 20-21 -0.025245374304418533 1106 22-23 -0.06322867919531205 1106 24-25 0.029851779225808173 1106 26-27 -0.14564242703857389 1106 28-29 -0.14796505426585327 1106 30-31 -0.034545025228688075 1106 32-33 0.2177770590481103 1106 34-35 0.12134709733162197 1106 36-37 0.038977271125169466 1106 38-39 -0.15462624023727045 1106 40-41 -0.13402518767534843 1106 42-43 0.045369331382737244 1106 44-45 0.12806302172828765 1106 46-47 -0.03485752125857999 1106 48-49 0.14990176326728744 1106 50-51 -0.003040379234889201 1106 52-53 -0.015412835422850435 1106 54-55 0.15079877287150367 1106 56-57 0.3144437695281894 1106 58-59 0.18570593867495688 1106 60-61 0.2952823704531653 1106 62-63 0.22310804179873145 1106 64-65 0.14493936549656894 1106 66-67 0.3219927148174193 1106 68-69 0.3648010205801029 1106 70-71 0.3510297622335372 1106 72-73 0.3756757142031901 1106 74-75 0.26445403036005644 1106 76-77 0.25881593887246623 1106 78-79 0.3541809604176862 1106 80-81 0.32595804302772535 1106 82-83 0.1757167577878036 1106 84-85 0.11948496121692287 1106 86-87 0.07766125190592632 1106 88-89 0.17750071323343874 1106 90-91 0.15576025962091578 1106 92-93 -0.08845998167808489 1106 94-95 -0.13054263896028218 1106 96-97 -0.0355649025075806 1106 98-99 -0.18342164884091616 1106 100-101 -0.08142294620908785 1107 1 -0.1110695163517228 1107 2 -0.02534741185128553 1107 3 0.01481881697075238 1107 4 -0.028479407619357744 1107 5 0.03287956549175419 1107 6 -0.008463034625400212 1107 7 -1.7092198547885573E-4 1107 8 0.04391530716780778 1107 9 0.08975042239884345 1107 10-11 0.05778070311737338 1107 12-13 -0.01628669771479707 1107 14-15 -0.05831334119588405 1107 16-17 0.08445232218355869 1107 18-19 -0.07201122534819149 1107 20-21 0.2520957824105423 1107 22-23 0.20868244373982492 1107 24-25 0.24123868026849493 1107 26-27 0.12634985046366864 1107 28-29 -0.0046802590926589005 1107 30-31 0.06621233603971888 1107 32-33 0.05022765849357569 1107 34-35 0.10794683080293765 1107 36-37 0.13038660255798362 1107 38-39 -0.19366646849516655 1107 40-41 -0.0824627486938212 1107 42-43 0.12361392758690215 1107 44-45 0.06319916614192067 1107 46-47 -0.1438792243085274 1107 48-49 -0.13278156062852275 1107 50-51 -0.29800489089186044 1107 52-53 -0.09451718189062319 1107 54-55 0.025505884208250507 1107 56-57 -0.025924586780632808 1107 58-59 0.14879098377981137 1107 60-61 0.3167761270431555 1107 62-63 0.15912956423400004 1107 64-65 0.09184414251065931 1107 66-67 0.19723983962704494 1107 68-69 0.4391056540787517 1107 70-71 0.46536373341329096 1107 72-73 0.27676721262159276 1107 74-75 0.33121311967157396 1107 76-77 0.27381671990890766 1107 78-79 0.31373120546786737 1107 80-81 0.09879298562154659 1107 82-83 0.25306072135245117 1107 84-85 0.3075536533714107 1107 86-87 0.21712934168606424 1107 88-89 0.45563694063219273 1107 90-91 0.41157348940183525 1107 92-93 0.5207002333021933 1107 94-95 0.43628296048517967 1107 96-97 0.4665066746002431 1107 98-99 0.5706269267438877 1107 100-101 0.5818124484095684 1108 1 0.08393514862107665 1108 2 0.17307529923139953 1108 3 0.08488436130559762 1108 4 0.1307457961431311 1108 5 0.14309614932336956 1108 6 0.11620176214021427 1108 7 0.19456822095046533 1108 8 0.16287358059975787 1108 9 0.2716344846219556 1108 10-11 0.20081346354901086 1108 12-13 0.36028149597253645 1108 14-15 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96-97 -3.4749392575682023 2316 98-99 -3.291888359220142 2316 100-101 -3.6204713381602467 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1664.0 3 569.0 4 534.0 5 555.0 6 558.0 7 667.0 8 851.0 9 1054.0 10 1384.0 11 1689.0 12 2550.0 13 3217.0 14 3683.0 15 4686.0 16 6098.0 17 8681.0 18 7679.0 19 4546.0 20 4125.0 21 4110.0 22 4419.0 23 4780.0 24 5430.0 25 6217.0 26 7112.0 27 8460.0 28 10220.0 29 12186.0 30 15432.0 31 19650.0 32 25462.0 33 34441.0 34 49471.0 35 73371.0 36 116710.0 37 190221.0 38 203452.0 39 42600.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.48732021728729 20.138140673815755 15.18714289735146 22.187396211545497 2 14.363653148578878 20.73466465220064 42.20606546414153 22.695616735078953 3 18.206952138335822 26.286485555496768 30.946685413432608 24.559876892734803 4 11.730305654042454 17.52970463688453 40.16512771896086 30.57486199011215 5 12.631746092522667 37.97665930536489 35.60171040466138 13.78988419745106 6 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31.354332569827058 20.769490514325593 28-29 20.209035309531757 27.314333568396282 31.64710841617286 20.8295227058991 30-31 20.47590399755081 27.27657459875638 31.47137760824531 20.7761437954475 32-33 20.26875844829391 27.380632149043382 31.387079020318676 20.963530382344036 34-35 20.70593637782301 27.463604956557802 31.091938530939657 20.73852013467953 36-37 20.78578655751026 27.129658496068142 31.11734125970303 20.967213686718566 38-39 20.342725813159227 27.65391344572195 31.134970447200295 20.86839029391853 40-41 20.588074744581412 27.444969991305495 31.281186954307437 20.685768309805656 42-43 20.410457840003602 27.452561563048665 30.920575788952416 21.216404807995318 44-45 20.62513645950332 27.62339878137443 30.631416410254104 21.120048348868146 46-47 20.712521172491797 27.791195619756117 30.27263979471827 21.223643413033813 48-49 20.759038225954665 27.86660896937748 30.107462138967563 21.266890665700295 50-51 20.732799855938595 27.91926933855556 29.98508739350148 21.362843412004366 52-53 20.96597022450918 28.171918119651195 29.688435548712683 21.17367610712694 54-55 20.800705906054393 28.07023270026511 29.754300348844996 21.374761044835502 56-57 20.79232049419709 28.272026772010733 29.579672452598267 21.35598028119391 58-59 20.96593476659465 27.92203370563312 29.695667857643304 21.41636367012892 60-61 20.99049820944461 27.969302513531176 29.926109691286072 21.114089585738142 62-63 20.783485451678366 27.866696381237947 30.183532499749568 21.16628566733412 64-65 20.687163504800623 27.990660438040987 29.93235064213709 21.389825415021296 66-67 20.689441699899486 28.2049550386131 29.488006357289105 21.61759690419831 68-69 20.610725691251115 28.04448231727703 29.800766136884775 21.544025854587083 70-71 20.73328725923658 27.96851503336245 29.7446939749489 21.55350373245207 72-73 20.819633934812437 27.81873683760289 29.72599702168229 21.635632205902382 74-75 20.675286199985702 28.21410420994396 29.459758658319906 21.65085093175043 76-77 20.67525842243837 28.062149709974776 29.598219662731 21.66437220485585 78-79 20.695394993858564 27.987489712675444 29.422085971072775 21.89502932239321 80-81 20.656563751876494 28.168624826422406 29.315995931515726 21.858815490185375 82-83 20.810682109088372 28.14376852578384 29.346924255506934 21.698625109620856 84-85 20.71995610023779 27.91073715017377 29.386219414388425 21.98308733520001 86-87 20.52973274161458 28.037844485280665 29.607062352785572 21.82536042031918 88-89 20.75371454299865 27.87038496172895 29.259286357496624 22.116614137775777 90-91 20.475591760101487 27.77521389360081 29.765400845781613 21.983793500516093 92-93 20.729909587303162 27.936921060141355 29.56736654938476 21.76580280317072 94-95 20.510611091005998 27.719185287034414 29.509959910466897 22.260243711492688 96-97 20.542089323711203 27.69378426758835 29.523096495277123 22.241029913423326 98-99 20.34260265411489 27.825078401675572 29.35887979911154 22.473439145097995 100-101 20.355242487204176 27.648608929204887 29.548809946113952 22.447338637476985 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 136.0 1 106.5 2 106.5 3 139.0 4 134.5 5 149.5 6 231.5 7 479.5 8 848.5 9 1025.0 10 953.5 11 921.5 12 1004.0 13 1069.0 14 1144.0 15 1239.0 16 1290.5 17 1370.5 18 1464.5 19 1608.5 20 1884.5 21 2208.5 22 2638.0 23 3194.5 24 3902.5 25 4976.5 26 6587.0 27 8388.5 28 9757.5 29 11296.0 30 13399.0 31 15872.0 32 18711.5 33 21560.5 34 24745.0 35 27666.0 36 31236.5 37 34596.5 38 35217.5 39 34879.5 40 33979.5 41 33318.0 42 32808.0 43 32872.5 44 33515.0 45 33449.5 46 32303.5 47 31452.5 48 33039.0 49 31941.0 50 28282.0 51 25964.5 52 23955.5 53 22605.0 54 21025.5 55 18551.0 56 16449.0 57 14367.0 58 12601.0 59 11026.5 60 9153.5 61 7298.5 62 5597.5 63 4148.5 64 2993.5 65 2236.5 66 1611.0 67 1177.5 68 842.0 69 540.5 70 380.0 71 284.5 72 195.5 73 135.5 74 106.0 75 88.0 76 65.0 77 36.0 78 22.5 79 14.5 80 8.0 81 6.0 82 7.0 83 5.5 84 2.0 85 2.0 86 4.5 87 4.0 88 1.0 89 0.5 90 1.5 91 2.0 92 1.5 93 0.5 94 0.0 95 1.0 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03680144774869676 2 0.03488822263637919 3 0.024871926460128386 4 0.04400417758330407 5 0.0647120258578001 6 0.0632489713601455 7 0.06594999504812324 8 0.06561236708712602 9 0.06572490974079176 10-11 0.071239499770413 12-13 0.06960763129225976 14-15 0.0626862580918168 16-17 0.05261369058873312 18-19 0.0407967119538305 20-21 0.05070046547641556 22-23 0.02802312076276909 24-25 0.015643428859537765 26-27 0.012042063942234107 28-29 0.015474614879039155 30-31 0.010747823425078105 32-33 0.00664001656627862 34-35 0.0081593423907661 36-37 0.004614248800295311 38-39 0.00647120258578001 40-41 0.006696287893111489 42-43 0.0036013649173036585 44-45 0.0011254265366573931 46-47 0.0019132251123175682 48-49 7.315272488273056E-4 50-51 0.004726791453961051 52-53 0.004051535531966615 54-55 0.005458318702788356 56-57 0.0181756385670169 58-59 0.007371543815105925 60-61 0.015812242840036374 62-63 0.011141722712908192 64-65 0.00827188504443184 66-67 0.013842746400885935 68-69 0.022395988079482124 70-71 0.014405459669214634 72-73 0.01474308763021185 74-75 0.016149870801033594 76-77 0.020370220313498815 78-79 0.0363512771340338 80-81 0.029317361279925094 82-83 0.02959871791408944 84-85 0.01851326652801412 86-87 0.020201406333000205 88-89 0.017106483357192375 90-91 0.016093599474200724 92-93 0.02661633759194735 94-95 0.018625809181679855 96-97 0.03623873448036806 98-99 0.05593369887187244 100-101 0.0638116846284742 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 888552.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.38759010539413 #Duplication Level Percentage of deduplicated Percentage of total 1 83.61531521514655 52.16558012175888 2 10.014573607042053 12.49570226552876 3 2.6980646936881127 5.049772625629492 4 1.0986446160300467 2.741671599055227 5 0.5589804218328674 1.7436720717124616 6 0.3499170118490058 1.3098287463684068 7 0.26310357556988206 1.1490078619542174 8 0.16569609416242398 0.8269904003736075 9 0.14065494511697468 0.789761075602907 >10 0.9516236762892996 11.65981064528266 >50 0.10061789948605096 4.352334404837502 >100 0.04154386529338827 4.325299545608361 >500 7.225019961891319E-4 0.32206200668637974 >1k 5.41876497141849E-4 1.068506629601208 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4352 0.48978562875329745 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2984 0.33582727853856614 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2146 0.24151653476667656 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.813566341643483E-4 0.0 24-25 0.0 0.0 0.0 0.0011254265366573931 0.0 26-27 0.0 0.0 0.0 0.0017444111318189594 0.0 28-29 0.0 0.0 0.0 0.0037139075709693974 0.0 30-31 0.0 0.0 0.0 0.007596629122437403 0.0 32-33 0.0 0.0 0.0 0.011929521288568367 0.0 34-35 0.0 0.0 0.0 0.01637495610836507 0.0 36-37 0.0 0.0 0.0 0.022170902772150648 0.0 38-39 0.0 0.0 0.0 0.0319058423142371 0.0 40-41 0.0 0.0 0.0 0.05075673680324843 0.0 42-43 0.0 0.0 0.0 0.06966390261909264 0.0 44-45 0.0 0.0 0.0 0.08840225445443824 0.0 46-47 0.0 0.0 0.0 0.12964913702293168 0.0 48-49 0.0 0.0 0.0 0.15401462154156426 0.0 50-51 0.0 0.0 0.0 0.181756385670169 0.0 52-53 0.0 0.0 0.0 0.21535036778939218 0.0 54-55 0.0 0.0 0.0 0.2610989565045152 0.0 56-57 0.0 0.0 0.0 0.3895101243371238 0.0 58-59 0.0 0.0 0.0 0.4972697152220692 0.0 60-61 0.0 0.0 0.0 0.5739112623684377 0.0 62-63 0.0 0.0 0.0 0.678182030989745 0.0 64-65 0.0 0.0 0.0 0.7798643185767407 0.0 66-67 0.0 0.0 0.0 0.9357921652306225 0.0 68-69 0.0 0.0 0.0 1.1274523044233764 0.0 70-71 0.0 0.0 0.0 1.2204125363512772 0.0 72-73 0.0 0.0 0.0 1.337963338105142 0.0 74-75 0.0 0.0 0.0 1.5456045341184308 0.0 76-77 0.0 0.0 0.0 1.7911163330902413 0.0 78-79 0.0 0.0 0.0 2.016145369094887 0.0 80-81 0.0 0.0 0.0 2.180626457427365 0.0 82-83 0.0 0.0 0.0 2.4511227255129695 0.0 84-85 0.0 0.0 0.0 2.8620722253734163 0.0 86-87 0.0 0.0 0.0 3.2485436980615656 0.0 88-89 0.0 0.0 0.0 3.66298202018565 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4595 0.0 36.402973 1 GCGTATC 70 2.0477959E-4 33.943115 1 GGTATCA 2745 0.0 32.89205 1 ATCAACG 5430 0.0 30.280006 3 CAACGCA 5455 0.0 30.146328 5 TCAACGC 5500 0.0 29.894625 4 AACGCAG 5560 0.0 29.744633 6 TATCAAC 5670 0.0 29.333553 2 CGTATCA 100 4.937413E-5 28.512215 2 TGCACCG 125 7.354214E-6 26.615898 5 ACGCAGA 6195 0.0 26.31219 7 GTACATG 3450 0.0 26.170633 1 CGCAGAG 6260 0.0 26.114893 8 GTATAGG 290 0.0 24.579494 1 ACATGGG 3345 0.0 24.43498 3 GCAGAGT 6465 0.0 24.404713 9 TACATGG 3660 0.0 24.019854 2 GAGTACT 3450 0.0 22.726488 12-13 AGAGTAC 5345 0.0 22.048737 10-11 AGTACTT 3750 0.0 21.41524 12-13 >>END_MODULE