FastQCFastQC Report
Thu 26 May 2016
SRR936918_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936918_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888552
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52560.5915241876671258No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40920.4605245388002052No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26340.29643734975555736No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13560.15260783837074252No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11000.12379691903231324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62350.030.0267811
ATACCGT804.4967426E-429.6830866
ATACCGA650.00584761529.2264236
GGTATCA42250.028.7913681
ATCAACG75600.024.6929573
CAACGCA76100.024.5279565
TCAACGC76350.024.4476434
GTACATG38050.024.2268031
TATCAAC77000.024.1891122
AACGCAG77550.024.129236
CTAATAC3000.023.7504793
ACGATCG601.7124008E-423.74780782-83
GGGCGTA1201.7121155E-423.7464719
ACATGGG37850.023.0916163
TCGTTAG1050.002197730422.6156859
TACCGTG1050.002197730422.6156857
AGTACTT46250.022.54052512-13
TACATGG40900.022.5373572
GAGTACT44150.022.26798412-13
TAAGGTG3850.022.2057425
Produced by FastQC (version 0.11.5)