##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936918_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 888552 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.440146440500946 33.0 31.0 34.0 31.0 34.0 2 32.52439699646166 34.0 31.0 34.0 31.0 34.0 3 32.64462856422584 34.0 31.0 34.0 31.0 34.0 4 36.26418937777418 37.0 35.0 37.0 35.0 37.0 5 36.199441338267206 37.0 35.0 37.0 35.0 37.0 6 36.24292669421711 37.0 37.0 37.0 35.0 37.0 7 36.20898945700421 37.0 36.0 37.0 35.0 37.0 8 36.192246486418355 37.0 36.0 37.0 35.0 37.0 9 37.9325002926109 39.0 38.0 39.0 35.0 39.0 10-11 37.845715276089635 39.0 38.0 39.0 35.0 39.0 12-13 37.786610687950734 39.0 37.0 39.0 35.0 39.0 14-15 39.06737365961699 40.0 38.0 41.0 36.0 41.0 16-17 39.015535950625285 40.0 38.0 41.0 36.0 41.0 18-19 39.06394054596692 40.0 38.5 41.0 36.0 41.0 20-21 39.14215206313193 40.0 39.0 41.0 36.0 41.0 22-23 39.084759811468544 40.0 38.5 41.0 36.0 41.0 24-25 39.00760732067454 40.0 38.0 41.0 35.0 41.0 26-27 38.839655979616275 40.0 38.0 41.0 35.0 41.0 28-29 38.69212550306566 40.0 38.0 41.0 35.0 41.0 30-31 38.6437445416813 40.0 38.0 41.0 35.0 41.0 32-33 38.54970896469762 40.0 38.0 41.0 35.0 41.0 34-35 38.42097930115514 40.0 38.0 41.0 35.0 41.0 36-37 38.26420175746608 40.0 38.0 41.0 34.0 41.0 38-39 38.11370128028523 40.0 38.0 41.0 34.0 41.0 40-41 37.904543009300525 40.0 38.0 41.0 33.5 41.0 42-43 37.67808974601374 40.0 37.0 41.0 33.0 41.0 44-45 37.39719341130288 40.0 37.0 41.0 33.0 41.0 46-47 36.996628784809445 40.0 37.0 41.0 31.5 41.0 48-49 36.591560764029566 40.0 37.0 41.0 31.0 41.0 50-51 36.36454647561426 40.0 36.0 41.0 30.0 41.0 52-53 36.16278507054173 39.0 36.0 41.0 30.0 41.0 54-55 35.91308724756683 39.0 36.0 40.5 29.0 41.0 56-57 35.68197584384482 39.0 35.0 40.0 28.5 41.0 58-59 35.36585365853659 38.5 35.0 40.0 28.0 41.0 60-61 35.06789923380961 38.0 35.0 40.0 27.0 41.0 62-63 34.763419586023105 38.0 34.0 40.0 26.5 41.0 64-65 34.38730260018547 37.5 34.0 40.0 26.0 41.0 66-67 33.9766271416867 37.0 34.0 39.5 26.0 41.0 68-69 33.54646998712512 36.5 33.0 39.0 25.0 40.0 70-71 33.163932442895856 36.0 33.0 39.0 24.0 40.0 72-73 32.790868739252176 35.5 32.5 38.5 22.5 40.0 74-75 32.318800137752206 35.0 32.0 37.5 21.5 39.5 76-77 30.861828570528232 34.0 30.5 36.0 20.0 38.5 78-79 31.51349105060818 35.0 31.5 36.0 20.5 39.0 80-81 31.07991710108131 35.0 31.0 36.0 15.5 38.5 82-83 30.96450235889402 34.5 31.0 36.0 18.0 37.0 84-85 30.654966169678307 34.0 31.0 35.0 17.5 37.0 86-87 30.311310986864022 34.0 31.0 35.0 12.5 36.5 88-89 29.957050347081545 34.0 31.0 35.0 7.0 36.0 90-91 29.548669070577752 34.0 30.0 35.0 3.5 36.0 92-93 29.18363472255985 34.0 30.0 35.0 2.0 35.0 94-95 28.728548807498044 34.0 29.0 35.0 2.0 35.0 96-97 28.15432974097183 34.0 29.0 35.0 2.0 35.0 98-99 27.47581852272011 33.5 28.0 35.0 2.0 35.0 100-101 24.33221297121609 30.0 20.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14884806529568806 1101 2 0.11056053481855344 1101 3 0.10994908575811024 1101 4 0.09037238118833102 1101 5 0.11096595098775452 1101 6 0.13667223225363756 1101 7 0.09720363848788338 1101 8 0.10445317175316404 1101 9 0.07701321911696368 1101 10-11 0.09874853620906521 1101 12-13 0.03748678916802817 1101 14-15 0.18690215996514326 1101 16-17 0.1343764643242551 1101 18-19 0.08137681126704166 1101 20-21 0.0467289868716918 1101 22-23 0.08686581352146305 1101 24-25 0.09364008982860383 1101 26-27 0.06628995568261331 1101 28-29 0.06734259683035759 1101 30-31 0.058710407464687364 1101 32-33 0.08714592349898709 1101 34-35 0.04689667560398192 1101 36-37 0.1681944945995255 1101 38-39 0.21234661030975133 1101 40-41 0.27623427364779474 1101 42-43 0.34801913433022236 1101 44-45 0.15157177877136974 1101 46-47 0.1664645977558905 1101 48-49 0.20338193099001955 1101 50-51 0.22494496658283225 1101 52-53 0.22532807753059814 1101 54-55 0.2289242119224255 1101 56-57 0.14813885145125028 1101 58-59 0.22241424109900976 1101 60-61 0.15434034018604592 1101 62-63 0.09486987329260188 1101 64-65 0.20363359652232305 1101 66-67 0.26441617420089614 1101 68-69 0.10223597529721218 1101 70-71 0.13893247306385348 1101 72-73 -0.060181463877960084 1101 74-75 -0.0037225200462600583 1101 76-77 -0.1126610057163262 1101 78-79 0.24208333301141494 1101 80-81 0.5459530251246179 1101 82-83 0.47569547696381775 1101 84-85 0.31045416287622984 1101 86-87 0.2131292148683599 1101 88-89 0.0865587465599802 1101 90-91 -0.12753616473146323 1101 92-93 0.07977159362079789 1101 94-95 0.21881864691124875 1101 96-97 0.3182761533577043 1101 98-99 0.26660949382478094 1101 100-101 0.550817319314838 1102 1 0.14407414929185336 1102 2 0.11025288491014607 1102 3 0.03498567214841586 1102 4 -0.025079971025490977 1102 5 0.033377012531005334 1102 6 0.05981539004598346 1102 7 0.0673778088647694 1102 8 0.08119004891868542 1102 9 0.11335754236822027 1102 10-11 0.10490116593415877 1102 12-13 0.034123641426802465 1102 14-15 0.058397898553359084 1102 16-17 0.09373020055738124 1102 18-19 0.15519805156409205 1102 20-21 -0.049302918818689534 1102 22-23 0.036118132133289294 1102 24-25 0.06787137034489632 1102 26-27 -6.460059757316117E-4 1102 28-29 -0.08824719936171022 1102 30-31 0.09088690345382133 1102 32-33 0.008779201860626529 1102 34-35 -0.01064859498792714 1102 36-37 -0.06432601662251614 1102 38-39 -0.11635281536129582 1102 40-41 0.007166795522259406 1102 42-43 0.04518348643794212 1102 44-45 0.12515091517044397 1102 46-47 0.16794776506880993 1102 48-49 0.27627524758129596 1102 50-51 0.21708662997036043 1102 52-53 0.15166932059835148 1102 54-55 0.23525811560552512 1102 56-57 0.2514780872415088 1102 58-59 0.29011122178227566 1102 60-61 0.23913436580905056 1102 62-63 0.07731559058480997 1102 64-65 0.06848004421593856 1102 66-67 0.24665040520877568 1102 68-69 0.086951669937541 1102 70-71 0.18484194514397956 1102 72-73 0.26620690305001204 1102 74-75 0.20275865030557583 1102 76-77 0.028064878583727193 1102 78-79 0.19513079878674588 1102 80-81 0.4201940325362159 1102 82-83 0.32863679430966997 1102 84-85 0.12121409867872401 1102 86-87 0.061759012180495176 1102 88-89 -0.003964751997934002 1102 90-91 0.030768319514649534 1102 92-93 0.14592792988251446 1102 94-95 0.249408443103313 1102 96-97 0.194866537584808 1102 98-99 0.37104982153272914 1102 100-101 0.5121982289564002 1103 1 0.1151293653547043 1103 2 0.08277865864172185 1103 3 0.062250746663600864 1103 4 0.03573252507727176 1103 5 0.07741060152312684 1103 6 0.03841955541356867 1103 7 0.1279187974120859 1103 8 0.07496226317321941 1103 9 0.09487814056821975 1103 10-11 0.09773154775952264 1103 12-13 0.1533628746582849 1103 14-15 0.18204555709298376 1103 16-17 0.11519683500689126 1103 18-19 0.21273167173411167 1103 20-21 0.19686739566240874 1103 22-23 0.15450257124284406 1103 24-25 0.17450823613442168 1103 26-27 0.07920254736965404 1103 28-29 0.06314554346217705 1103 30-31 0.17451447822881505 1103 32-33 0.0719358864026205 1103 34-35 0.1472869602904865 1103 36-37 0.26614154796769895 1103 38-39 0.17676167508787444 1103 40-41 0.20495550288647735 1103 42-43 0.21947899578091068 1103 44-45 0.18024681319792535 1103 46-47 0.03344110339060791 1103 48-49 0.1733368603102008 1103 50-51 0.20323779602385628 1103 52-53 0.2657538075140238 1103 54-55 0.1899299941779944 1103 56-57 0.12011194955989879 1103 58-59 0.09397948995379579 1103 60-61 0.12465885605036675 1103 62-63 0.12464884077707694 1103 64-65 0.01485665247600565 1103 66-67 0.022350089272705986 1103 68-69 0.014155817637650614 1103 70-71 0.11544513360400543 1103 72-73 0.16780749854792276 1103 74-75 0.08449112832516192 1103 76-77 0.2275040660876222 1103 78-79 0.18591448131256882 1103 80-81 0.5000146556785339 1103 82-83 0.22990482552914315 1103 84-85 0.27714647381122504 1103 86-87 0.3090984979529807 1103 88-89 0.015783200653224583 1103 90-91 0.05135807549561022 1103 92-93 -0.04864295827195875 1103 94-95 0.16805880372770332 1103 96-97 0.41345415268322583 1103 98-99 0.39721522549439925 1103 100-101 0.5759407982233036 1104 1 0.10575338329602602 1104 2 0.093989007278509 1104 3 0.021489954731478633 1104 4 0.07591717591672165 1104 5 -0.026280510667255896 1104 6 0.056014475848961354 1104 7 -0.018052619323619012 1104 8 0.0439589682175523 1104 9 0.2116389658308151 1104 10-11 0.14748978276795555 1104 12-13 0.16940167847509713 1104 14-15 0.10815486677120134 1104 16-17 0.1193515384132624 1104 18-19 0.13249569196094768 1104 20-21 0.15252357751349166 1104 22-23 0.16750711132988272 1104 24-25 0.19193918252950226 1104 26-27 0.14892603535639637 1104 28-29 0.12938897259682847 1104 30-31 0.161719443993654 1104 32-33 0.2288179750807373 1104 34-35 0.23324090233354866 1104 36-37 0.18620197135998495 1104 38-39 0.4262977267596355 1104 40-41 0.4775160691236593 1104 42-43 0.24713882731916925 1104 44-45 0.34340634000038506 1104 46-47 0.3054154103547688 1104 48-49 0.6109329830234884 1104 50-51 0.6528965752713489 1104 52-53 0.5547739748330187 1104 54-55 0.5120044711275895 1104 56-57 0.5952887796411233 1104 58-59 0.441329776595893 1104 60-61 0.5374374631221386 1104 62-63 0.6145044721991368 1104 64-65 0.5871849767148873 1104 66-67 0.46198085901625063 1104 68-69 0.6037311754641266 1104 70-71 0.46387479201423076 1104 72-73 0.4834700250932613 1104 74-75 0.670769744919923 1104 76-77 0.6155490788960485 1104 78-79 0.9397936131012763 1104 80-81 0.8879456901612919 1104 82-83 0.8182725487719118 1104 84-85 0.8005926784222588 1104 86-87 0.8448549569231645 1104 88-89 0.7740905243111982 1104 90-91 0.9373735003639574 1104 92-93 0.7996414744909437 1104 94-95 0.9783339944256433 1104 96-97 1.030191468056195 1104 98-99 1.0452439063693717 1104 100-101 0.5783426756814265 1105 1 0.15714150693327866 1105 2 0.061839783074610466 1105 3 0.14576420540925028 1105 4 0.11925724166118812 1105 5 0.1664882468336728 1105 6 0.09791582344018224 1105 7 0.1121866720447997 1105 8 0.07830724836916403 1105 9 0.1192323628204619 1105 10-11 0.1800228274380018 1105 12-13 0.19657503143494637 1105 14-15 0.17110223467975771 1105 16-17 0.2504720750802889 1105 18-19 0.31464325267234017 1105 20-21 0.21084400187448438 1105 22-23 -0.024614912742876527 1105 24-25 0.17718724983522094 1105 26-27 0.2385744866448718 1105 28-29 0.016281625396729282 1105 30-31 0.1455308217955249 1105 32-33 0.038914887719791125 1105 34-35 0.03174002636274054 1105 36-37 0.08837887445627501 1105 38-39 0.12986539073739323 1105 40-41 0.25441048279123635 1105 42-43 0.2166121169039954 1105 44-45 0.16052765404586467 1105 46-47 0.18229505545473046 1105 48-49 0.11604396231747671 1105 50-51 0.4088330120054806 1105 52-53 0.42834408130465107 1105 54-55 0.2555227334352068 1105 56-57 0.16642986246925773 1105 58-59 0.4229331723926961 1105 60-61 0.47874214435234563 1105 62-63 0.4295533180193516 1105 64-65 0.5548937470531072 1105 66-67 0.4519836747816157 1105 68-69 0.3703809096704802 1105 70-71 0.4830219964947915 1105 72-73 0.542822626268098 1105 74-75 0.39213231568935925 1105 76-77 0.1655398688277785 1105 78-79 0.35141631352574976 1105 80-81 0.6528820270858766 1105 82-83 0.39537218061261825 1105 84-85 0.3387852491835801 1105 86-87 0.23841334591587326 1105 88-89 0.24734602783415127 1105 90-91 0.27698239298916505 1105 92-93 0.41883314696631047 1105 94-95 0.48180420208631247 1105 96-97 0.681133532263761 1105 98-99 0.6945829318224312 1105 100-101 1.060781063570552 1106 1 0.08280177914780751 1106 2 0.0759539459980445 1106 3 0.06440591907739446 1106 4 0.006278502088761684 1106 5 0.03969508428174606 1106 6 0.10091955541356867 1106 7 -0.03264154741550129 1106 8 0.039761113747935894 1106 9 0.04459078424637397 1106 10-11 0.04277465120780022 1106 12-13 -0.11872764258309587 1106 14-15 0.013762511115977816 1106 16-17 0.02216522581148439 1106 18-19 0.13676184414790526 1106 20-21 0.0020038899152865497 1106 22-23 0.016391939058934213 1106 24-25 -0.049270499497758635 1106 26-27 0.14170254736965404 1106 28-29 0.12025761242769306 1106 30-31 -0.05734615395509479 1106 32-33 -0.1602839411835788 1106 34-35 0.010613109715777114 1106 36-37 -0.05066879685988113 1106 38-39 -0.06749119847534502 1106 40-41 -0.019721221251451482 1106 42-43 -0.022798303069656356 1106 44-45 0.1671361810140155 1106 46-47 0.29942529879290447 1106 48-49 0.1993785269768651 1106 50-51 -0.1916975488037309 1106 52-53 -0.03830510053195724 1106 54-55 -0.03923667248866991 1106 56-57 -0.16149724584240488 1106 58-59 -0.2258840157933335 1106 60-61 -0.11492447728296185 1106 62-63 -0.05441006726890407 1106 64-65 -0.09164478430560052 1106 66-67 -0.1725134164744233 1106 68-69 -0.06289159615545259 1106 70-71 0.03157300716721778 1106 72-73 -0.2520919267394319 1106 74-75 -0.07517122799668385 1106 76-77 -0.22598012931467792 1106 78-79 -0.2699044842046696 1106 80-81 0.17889396602336305 1106 82-83 0.08694505541419772 1106 84-85 0.13759906001812183 1106 86-87 0.3184375784127482 1106 88-89 0.0901366489290858 1106 90-91 -0.0075499704814028235 1106 92-93 0.21213290379700922 1106 94-95 0.0791579416587389 1106 96-97 0.18823863544184505 1106 98-99 0.2267770071035926 1106 100-101 -0.0993465580985351 1107 1 0.06616082159713699 1107 2 -0.08273679286241986 1107 3 -0.08172355723768021 1107 4 0.08663982906966794 1107 5 0.12003566498233198 1107 6 0.034138499452311066 1107 7 0.07136443727618769 1107 8 0.04994143553494723 1107 9 0.025296743554378054 1107 10-11 0.00445383072902672 1107 12-13 0.033416424468882155 1107 14-15 0.0527002283417346 1107 16-17 0.06883459356249233 1107 18-19 0.15821532854670295 1107 20-21 0.08349344843443873 1107 22-23 -0.05943022382621166 1107 24-25 0.03169613072054034 1107 26-27 2.458656029489248E-4 1107 28-29 0.12239081819174658 1107 30-31 0.0075878728735148115 1107 32-33 0.08863908279423782 1107 34-35 0.005368572089345491 1107 36-37 0.020422603928963667 1107 38-39 0.18931236109821015 1107 40-41 0.4268748022967941 1107 42-43 0.06631978475788713 1107 44-45 -0.14781981246433418 1107 46-47 0.05479919557893709 1107 48-49 0.07455731109838837 1107 50-51 0.14677008211750575 1107 52-53 -0.02894303644792018 1107 54-55 0.07106397772415818 1107 56-57 0.12099997171529253 1107 58-59 -0.02461397732728443 1107 60-61 0.11886413520580419 1107 62-63 -0.013910277172449526 1107 64-65 0.314001743011147 1107 66-67 0.21894265510756838 1107 68-69 0.27767873129406695 1107 70-71 0.3063532186328288 1107 72-73 0.20119721294893367 1107 74-75 0.1371089834689272 1107 76-77 0.1973702973264757 1107 78-79 0.4651801606190098 1107 80-81 0.5634836290061394 1107 82-83 0.4335800087472741 1107 84-85 0.4396972411795268 1107 86-87 0.45328107823701913 1107 88-89 0.13495593662386085 1107 90-91 0.1422833270544288 1107 92-93 0.40668175762603553 1107 94-95 0.525180391574775 1107 96-97 0.6363924605677873 1107 98-99 0.5892163547429128 1107 100-101 0.5588438292955367 1108 1 0.13374424832772291 1108 2 0.04776362082384367 1108 3 0.08110013026195872 1108 4 0.046422775806824745 1108 5 0.03252048181614242 1108 6 0.055727934902584764 1108 7 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-0.9244959227253773 2316 76-77 -1.0045442255396324 2316 78-79 -0.9222445392394967 2316 80-81 -1.0079395966217497 2316 82-83 -1.1082915298467846 2316 84-85 -1.207778068281815 2316 86-87 -0.9590099665180247 2316 88-89 -0.845385536985436 2316 90-91 -0.6188369470056081 2316 92-93 -0.7894848191370407 2316 94-95 -0.50706910924848 2316 96-97 -0.7341859066511951 2316 98-99 -1.2679922658347493 2316 100-101 -1.6578129997530198 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 2.0 5 9.0 6 24.0 7 63.0 8 166.0 9 319.0 10 629.0 11 1075.0 12 1844.0 13 2783.0 14 3512.0 15 5051.0 16 6826.0 17 10471.0 18 9444.0 19 5152.0 20 3782.0 21 3567.0 22 3640.0 23 4121.0 24 4489.0 25 5408.0 26 6288.0 27 7765.0 28 9782.0 29 12362.0 30 15756.0 31 20286.0 32 26518.0 33 36242.0 34 51960.0 35 77828.0 36 127855.0 37 216719.0 38 189773.0 39 17039.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.34024206838792 21.009653917851644 15.415649793323308 21.234454220437126 2 15.00230728539432 21.83907528503416 40.97873920922014 22.17987822035138 3 18.780031267973737 26.733104545111765 31.002567395539867 23.484296791374632 4 12.467362630436936 18.568637513617663 39.20367069119196 29.76032916475344 5 12.848240242295187 38.57229519765268 34.8101244452748 13.769340114777329 6 29.567205971063032 38.77296995561318 17.998496430147025 13.661327643176763 7 26.55849066796316 34.47339041496727 22.087508665784334 16.880610251285237 8 25.488463802302853 35.59975735776562 20.404568786710048 18.50721005322148 9 26.45557368746835 17.306285521355015 21.47937651229531 34.75876427888132 10-11 23.754828079842262 28.14798683701123 28.253608117476524 19.84357696566999 12-13 25.281877302553653 25.65647573607157 28.782631057400724 20.279015903974052 14-15 22.369034113929178 26.774347477694043 27.55584366474894 23.300774743627837 16-17 20.687027883567872 30.076855378188334 28.16453060710009 21.071586131143704 18-19 20.389618136410242 28.250803872996507 31.625719884165754 19.733858106427498 20-21 21.35479855673027 27.369960541923927 31.671352266307125 19.603888635038683 22-23 21.09842755404298 27.19525700240391 31.463268328696575 20.24304711485653 24-25 21.01439488582008 27.427603515998694 31.66537236497057 19.892629233210656 26-27 20.873293048163248 27.581235319341946 31.54066577452535 20.00480585796945 28-29 20.408954568732167 27.728380332813725 31.754728554787082 20.10793654366703 30-31 20.767023850868608 27.566882680834702 31.499323700973946 20.16676976732274 32-33 20.776233995632193 27.782845354745078 31.312598721620084 20.12832192800264 34-35 21.036682472029238 27.594238489528056 31.374638864534948 19.99444017390776 36-37 21.194169626520896 27.23868816033921 31.347571775175272 20.219570437964627 38-39 20.63893761385181 27.96101927932825 31.4619571059004 19.938086000919537 40-41 20.9308476663247 27.869379237923624 31.273168066153396 19.926605029598285 42-43 20.92451724838473 27.96664394982636 30.7977325179282 20.31110628386071 44-45 21.142739773050202 27.88145488718631 30.54368347453216 20.432121865231327 46-47 21.392794057153917 28.394129408050713 29.833809869216644 20.379266665578726 48-49 21.34777590639826 28.500203425768728 29.69886796232656 20.453152705506454 50-51 21.292172705367022 28.649246667769624 29.41495904983461 20.643621577028743 52-53 21.614745560411993 28.689836878869883 29.2685575819784 20.426859978739724 54-55 21.23095726865107 28.708661966758502 29.232393351700793 20.827987412889634 56-57 21.393156049301297 28.962794437819944 29.00314168298095 20.640907829897813 58-59 21.498292701043738 28.4679439708825 29.163749890269187 20.870013437804573 60-61 21.44640943917745 28.768434486670447 29.403456409979384 20.38169966417272 62-63 21.243643439511878 28.54283465036596 29.7084408820883 20.50508102803386 64-65 21.163705533763668 28.61821926828033 29.38325520144521 20.8348199965108 66-67 21.11903545358576 28.873536647676758 28.98568666422071 21.02174123451677 68-69 21.199478693041137 28.701425716009254 29.156556402891947 20.94253918805766 70-71 21.321954541106027 28.76819790769785 28.982211492467602 20.927636058728524 72-73 21.262564298819264 28.58131760521369 29.21012347624461 20.945994619722434 74-75 21.353954755999904 28.88377911599804 28.689911631317504 21.072354496684557 76-77 21.358643078902187 28.749599751944686 28.84875434926841 21.04300281988472 78-79 21.4733686984142 28.676161886978065 28.57722520307908 21.273244211528656 80-81 21.574837178366078 28.941847889086592 28.32522460775939 21.15809032478794 82-83 21.456491962214585 28.89894213897496 28.500682618056906 21.14388328075355 84-85 21.35029359713465 28.824063992076958 28.538147932440307 21.28749447834809 86-87 21.160832455500636 28.96538413058549 28.555616328588535 21.31816708532534 88-89 21.166762703929123 28.858559621133 28.427911279576474 21.5467663953614 90-91 20.895096568419465 28.83963711297509 28.80761833162454 21.457647986980906 92-93 21.138021980742792 28.84096064320486 28.561122862459705 21.45989451359264 94-95 21.02554117937686 28.726497405028777 28.437485756216198 21.810475659378163 96-97 21.232979234638123 28.653408317173994 28.34430817953355 21.76930426865433 98-99 20.87844191185532 28.861612255015327 28.226364174011493 22.033581659117853 100-101 20.5887980156189 29.149705302749794 28.05116038800773 22.210336293623577 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 294.0 1 199.5 2 123.5 3 150.0 4 173.0 5 194.0 6 268.5 7 527.0 8 908.0 9 1165.0 10 1160.5 11 1093.5 12 1161.5 13 1290.0 14 1399.5 15 1512.5 16 1630.5 17 1703.0 18 1817.0 19 1966.5 20 2172.0 21 2472.5 22 2847.0 23 3340.5 24 4075.5 25 5125.0 26 6490.0 27 8152.0 28 9653.5 29 11376.5 30 13726.0 31 16198.5 32 18832.5 33 21695.5 34 24704.0 35 27353.5 36 30539.5 37 33282.5 38 34541.5 39 34731.5 40 33875.0 41 33191.0 42 32455.0 43 32417.5 44 33044.5 45 33012.5 46 32029.5 47 31625.0 48 33122.0 49 31835.5 50 28265.0 51 25944.0 52 23798.0 53 22276.0 54 20758.0 55 18429.0 56 16242.0 57 14350.0 58 12841.5 59 11352.0 60 9404.5 61 7444.5 62 5634.0 63 4238.0 64 3144.5 65 2303.5 66 1649.5 67 1234.0 68 850.5 69 513.5 70 371.0 71 286.0 72 208.5 73 137.0 74 110.5 75 100.0 76 65.0 77 35.0 78 19.5 79 9.0 80 8.0 81 6.5 82 3.0 83 3.0 84 3.0 85 2.0 86 1.5 87 1.0 88 2.0 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02340887196247378 2 0.006977644527275838 3 0.01159189332757115 4 0.0 5 0.005514590029621226 6 0.0 7 0.0 8 1.1254265366573933E-4 9 2.2508530733147866E-4 10-11 0.0 12-13 5.6271326832869664E-5 14-15 0.0 16-17 0.0 18-19 0.004614248800295311 20-21 0.0015755971513203503 22-23 0.0 24-25 0.004726791453961051 26-27 0.005964760644284184 28-29 0.007540357795604534 30-31 0.029429903933590833 32-33 6.189845951615662E-4 34-35 0.003938992878300876 36-37 0.015249529571707677 38-39 0.0069213732004429675 40-41 0.008046799737100362 42-43 0.008722055659094797 44-45 0.00332000828313931 46-47 0.002419667053813395 48-49 0.002419667053813395 50-51 0.003713907570969397 52-53 0.004557977473462442 54-55 0.0027010236879777437 56-57 0.0017444111318189596 58-59 0.0015755971513203503 60-61 0.0 62-63 1.6881398049860898E-4 64-65 0.010916637405576713 66-67 0.0011254265366573931 68-69 0.0018006824586518293 70-71 0.006414931258947141 72-73 0.013730203747220197 74-75 0.006583745239445749 76-77 0.004557977473462442 78-79 0.01181697863490263 80-81 3.3762796099721796E-4 82-83 0.0068651018736100985 84-85 5.064419414958269E-4 86-87 0.0 88-89 9.003412293259146E-4 90-91 0.0012379691903231325 92-93 1.6881398049860898E-4 94-95 7.315272488273056E-4 96-97 5.627132683286966E-4 98-99 1.6881398049860898E-4 100-101 0.0014630544976546112 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 888552.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.42761317255641 #Duplication Level Percentage of deduplicated Percentage of total 1 81.93757023832077 48.693542284220975 2 10.848677917656383 12.894220694482767 3 3.084053465017431 5.498338089676143 4 1.2413418716069669 2.95079938243024 5 0.6646060031772684 1.9747974234488752 6 0.42738876528732345 1.5239216530674937 7 0.2766801668463379 1.1509709349503774 8 0.20449828480009133 0.972227597084088 9 0.1526417873101363 0.816402338121097 >10 1.0025624038749645 11.541950269768607 >50 0.10241180114456822 4.239995094085618 >100 0.05604736639479805 5.922432124410241 >500 5.699732111052641E-4 0.19024856183819214 >1k 7.599642814736855E-4 1.036713528070196 >5k 1.8999107036842138E-4 0.5934400243451263 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5256 0.5915241876671258 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4092 0.4605245388002052 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2634 0.29643734975555736 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1356 0.15260783837074252 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1100 0.12379691903231324 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.1254265366573931E-4 0.0 18-19 0.0 0.0 0.0 1.1254265366573931E-4 0.0 20-21 0.0 0.0 0.0 1.1254265366573931E-4 0.0 22-23 0.0 0.0 0.0 3.9389928783008764E-4 0.0 24-25 0.0 0.0 0.0 0.0012379691903231325 0.0 26-27 0.0 0.0 0.0 0.0018569537854846987 0.0 28-29 0.0 0.0 0.0 0.004051535531966615 0.0 30-31 0.0 0.0 0.0 0.00838442769809758 0.0 32-33 0.0 0.0 0.0 0.012829862517894282 0.0 34-35 0.0 0.0 0.0 0.017331568664523855 0.0 36-37 0.0 0.0 0.0 0.02346514328930665 0.0 38-39 0.0 0.0 0.0 0.03336889681189171 0.0 40-41 0.0 0.0 0.0 0.052669961915566 0.0 42-43 0.0 0.0 0.0 0.07264628294123474 0.0 44-45 0.0 0.0 0.0 0.09172226273757754 0.0 46-47 0.0 0.0 0.0 0.13308168795973674 0.0 48-49 0.0 0.0 0.0 0.15739090115153642 0.0 50-51 0.0 0.0 0.0 0.18558283589480412 0.0 52-53 0.0 0.0 0.0 0.21940190332135878 0.0 54-55 0.0 0.0 0.0 0.26531930601698045 0.0 56-57 0.0 0.0 0.0 0.3942931871179177 0.0 58-59 0.0 0.0 0.0 0.5028405765785233 0.0 60-61 0.0 0.0 0.0 0.5799322943395546 0.0 62-63 0.0 0.0 0.0 0.6847095049023579 0.0 64-65 0.0 0.0 0.0 0.7867294204503507 0.0 66-67 0.0 0.0 0.0 0.9425447244505667 0.0 68-69 0.0 0.0 0.0 1.1348801195653153 0.0 70-71 0.0 0.0 0.0 1.2275589948590515 0.0 72-73 0.0 0.0 0.0 1.3440969127299247 0.0 74-75 0.0 0.0 0.0 1.5506126822065562 0.0 76-77 0.0 0.0 0.0 1.795955667197868 0.0 78-79 0.0 0.0 0.0 2.0205908039146836 0.0 80-81 0.0 0.0 0.0 2.1833837524421753 0.0 82-83 0.0 0.0 0.0 2.4555118890059333 0.0 84-85 0.0 0.0 0.0 2.868487156632364 0.0 86-87 0.0 0.0 0.0 3.2565342264718327 0.0 88-89 0.0 0.0 0.0 3.6730545876887337 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6235 0.0 30.026781 1 ATACCGT 80 4.4967426E-4 29.683086 6 ATACCGA 65 0.005847615 29.226423 6 GGTATCA 4225 0.0 28.791368 1 ATCAACG 7560 0.0 24.692957 3 CAACGCA 7610 0.0 24.527956 5 TCAACGC 7635 0.0 24.447643 4 GTACATG 3805 0.0 24.226803 1 TATCAAC 7700 0.0 24.189112 2 AACGCAG 7755 0.0 24.12923 6 CTAATAC 300 0.0 23.750479 3 ACGATCG 60 1.7124008E-4 23.747807 82-83 GGGCGTA 120 1.7121155E-4 23.746471 9 ACATGGG 3785 0.0 23.091616 3 TCGTTAG 105 0.0021977304 22.615685 9 TACCGTG 105 0.0021977304 22.615685 7 AGTACTT 4625 0.0 22.540525 12-13 TACATGG 4090 0.0 22.537357 2 GAGTACT 4415 0.0 22.267984 12-13 TAAGGTG 385 0.0 22.205742 5 >>END_MODULE