FastQCFastQC Report
Thu 26 May 2016
SRR936917_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936917_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences941004
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44240.47013615244993645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31940.33942469957619736No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21280.22614144041895679No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52900.032.6078721
GGTATCA34900.032.269661
ATCAACG64350.026.3655823
CAACGCA64800.026.1824865
TATCAAC65300.026.0547892
TCAACGC65550.025.8829154
AACGCAG66300.025.6631686
GTACATG40800.024.8079531
TACATGG41300.023.7047232
ACATGGG40000.023.0493893
GTATAGG3300.023.0397381
ACGCAGA73700.022.8917277
CGCAGAG73200.022.853328
GAGTACT38150.021.98183412-13
GGGCGTA1302.9338783E-421.9308939
TCGCGGG652.9396132E-421.9262386-87
CATGGGG15700.021.4919784
GCAGAGT77850.021.2407119
AGTACTT40900.020.62000812-13
AGAGTAC62200.020.20615610-11
Produced by FastQC (version 0.11.5)