##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936917_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 941004 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01188517795886 33.0 31.0 34.0 30.0 34.0 2 32.15470072390766 33.0 31.0 34.0 30.0 34.0 3 32.16027349511798 34.0 31.0 34.0 30.0 34.0 4 35.720296619355494 37.0 35.0 37.0 35.0 37.0 5 35.718147850593624 37.0 35.0 37.0 35.0 37.0 6 35.81048858453312 37.0 35.0 37.0 35.0 37.0 7 35.761614190800465 37.0 35.0 37.0 35.0 37.0 8 35.723005428244726 37.0 35.0 37.0 35.0 37.0 9 37.390088671249 39.0 37.0 39.0 35.0 39.0 10-11 37.265355938975816 39.0 37.0 39.0 34.0 39.0 12-13 37.20053687338205 39.0 37.0 39.0 34.0 39.0 14-15 38.42288502493082 40.0 38.0 41.0 34.0 41.0 16-17 38.34949320087906 40.0 38.0 41.0 34.0 41.0 18-19 38.327317418416925 40.0 38.0 41.0 34.0 41.0 20-21 38.24925983311442 40.0 38.0 41.0 34.0 41.0 22-23 38.10939220237108 40.0 38.0 41.0 33.5 41.0 24-25 38.22299533264471 40.0 38.0 41.0 34.0 41.0 26-27 38.180086907175735 40.0 38.0 41.0 34.0 41.0 28-29 38.08949802551318 40.0 38.0 41.0 33.5 41.0 30-31 37.909213988463385 40.0 38.0 41.0 33.0 41.0 32-33 37.69839660617808 40.0 37.0 41.0 33.0 41.0 34-35 37.50899252287982 40.0 37.0 41.0 33.0 41.0 36-37 37.33135725246652 40.0 37.0 41.0 32.0 41.0 38-39 37.1926320185674 40.0 37.0 41.0 31.5 41.0 40-41 37.0078830695725 40.0 36.0 41.0 31.0 41.0 42-43 36.78492439989628 39.0 36.0 41.0 31.0 41.0 44-45 36.52157057780838 39.0 36.0 41.0 30.0 41.0 46-47 36.1665704927928 39.0 35.5 41.0 30.0 41.0 48-49 35.925021041355826 39.0 35.5 40.5 29.0 41.0 50-51 35.259354901785755 38.5 35.0 40.0 28.0 40.5 52-53 35.467243497370895 38.5 35.5 40.0 28.0 40.5 54-55 35.88735595172815 39.0 36.0 41.0 28.5 41.0 56-57 35.80960814194201 39.0 35.5 41.0 28.0 41.0 58-59 35.59498896922861 39.0 35.0 41.0 28.0 41.0 60-61 35.34715155302209 39.0 35.0 41.0 27.5 41.0 62-63 35.04388504193393 38.5 35.0 40.0 26.0 41.0 64-65 34.72637151382991 38.0 34.5 40.0 26.0 41.0 66-67 34.37303029530162 37.5 34.0 40.0 26.0 41.0 68-69 33.99128218371017 37.0 34.0 39.5 25.0 41.0 70-71 33.593340198341345 36.5 34.0 39.0 24.0 41.0 72-73 33.18568146362821 36.0 33.0 39.0 23.5 40.5 74-75 32.74063234587739 35.5 33.0 38.5 22.0 40.0 76-77 32.29475379488291 35.0 32.0 37.5 20.5 39.5 78-79 31.85437362646705 35.0 32.0 37.0 20.0 39.0 80-81 31.38988144577494 35.0 31.5 36.5 18.0 39.0 82-83 30.963068701089476 35.0 31.0 36.0 16.5 37.5 84-85 30.549408397838903 34.5 31.0 35.5 11.0 37.0 86-87 30.187643198116056 34.0 31.0 35.0 7.0 37.0 88-89 29.819301512002074 34.0 30.5 35.0 4.0 36.0 90-91 29.42008588698879 34.0 30.0 35.0 2.0 36.0 92-93 29.071892361775298 34.0 29.5 35.0 2.0 36.0 94-95 28.667797373868762 34.0 29.0 35.0 2.0 35.0 96-97 28.16901522203944 34.0 29.0 35.0 2.0 35.0 98-99 27.552509872434122 34.0 28.5 35.0 2.0 35.0 100-101 25.06338761578059 31.5 22.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21474067150697707 1101 2 0.1590052290401971 1101 3 0.11055775019579528 1101 4 0.04558817077534627 1101 5 0.042639104269632355 1101 6 0.07365928862444093 1101 7 0.04415334405850757 1101 8 0.04548178662101776 1101 9 0.11906972330585575 1101 10-11 0.1375256020135467 1101 12-13 0.2354416217122477 1101 14-15 0.24744258740074798 1101 16-17 0.1240807028306179 1101 18-19 0.19848460349320618 1101 20-21 0.22979140361486827 1101 22-23 0.27592926337166546 1101 24-25 0.18908453227623312 1101 26-27 0.1520844387210616 1101 28-29 0.12806006913346835 1101 30-31 -0.001359073819408252 1101 32-33 0.11690490299948664 1101 34-35 0.004075570789773053 1101 36-37 0.11508976998841547 1101 38-39 0.2875894747700727 1101 40-41 0.13660328317177317 1101 42-43 0.23457395363987388 1101 44-45 0.12964206861112615 1101 46-47 0.3924851114693837 1101 48-49 0.2907483884450457 1101 50-51 0.34190536006080663 1101 52-53 0.3878287763658932 1101 54-55 0.29079009018695245 1101 56-57 0.23502681874528975 1101 58-59 0.14656264861439183 1101 60-61 0.21327413972988296 1101 62-63 0.33635174289089065 1101 64-65 0.1577574297316957 1101 66-67 0.2333805878274049 1101 68-69 0.27311130812120865 1101 70-71 0.2602331587253772 1101 72-73 0.3036322689207509 1101 74-75 0.3702391581004534 1101 76-77 0.39929850299844816 1101 78-79 0.3131793370614453 1101 80-81 0.28853392148240076 1101 82-83 0.33466027821286204 1101 84-85 0.18113201263487966 1101 86-87 0.0849837739280268 1101 88-89 0.24645283294179876 1101 90-91 0.3214679731609884 1101 92-93 0.41912418408836416 1101 94-95 0.5700398675326461 1101 96-97 0.35066220930018943 1101 98-99 0.010729766362391757 1101 100-101 0.19957029735300935 1102 1 0.1592914908963543 1102 2 0.20569841130171795 1102 3 0.05436371682064589 1102 4 0.17841271816564586 1102 5 0.1745227039567041 1102 6 0.1452827083148378 1102 7 0.1824192693288822 1102 8 0.05862784757677275 1102 9 0.2494025302971039 1102 10-11 0.23316141161871684 1102 12-13 0.14170336430838404 1102 14-15 0.16588172302247273 1102 16-17 0.3435825498190752 1102 18-19 0.3353760675113264 1102 20-21 0.36328303313301546 1102 22-23 0.3813123261076683 1102 24-25 -0.06912373492212254 1102 26-27 0.1689026597395653 1102 28-29 -0.010538498860135803 1102 30-31 0.02478546355054334 1102 32-33 0.24224909581727871 1102 34-35 0.13767166449645885 1102 36-37 0.10707862440383309 1102 38-39 0.14224180152378807 1102 40-41 0.26527390322191735 1102 42-43 0.25638900223240313 1102 44-45 -0.03921287041463728 1102 46-47 0.1405187720382841 1102 48-49 0.10253882763610989 1102 50-51 0.0449265054605803 1102 52-53 0.2468206019789747 1102 54-55 0.20889042303500815 1102 56-57 0.33349394152946843 1102 58-59 0.33337182462550885 1102 60-61 0.29762987032213317 1102 62-63 0.42322924412803076 1102 64-65 0.3586489943639677 1102 66-67 0.3258129145400517 1102 68-69 0.23214799943492181 1102 70-71 -0.040857272868287 1102 72-73 -0.01809587162696147 1102 74-75 0.2329234206089552 1102 76-77 0.30092018760797146 1102 78-79 0.16169702359290383 1102 80-81 0.2509019167350566 1102 82-83 0.09079491843511889 1102 84-85 0.022931650834586037 1102 86-87 0.16117230432479346 1102 88-89 0.18879501758315342 1102 90-91 0.04686524507998868 1102 92-93 0.03242855834017533 1102 94-95 0.1776954755398883 1102 96-97 0.059739454279235105 1102 98-99 0.13411198311813521 1102 100-101 0.49381152287450547 1103 1 -0.005549038382429927 1103 2 0.18310764929985623 1103 3 0.097893461046894 1103 4 0.1090158034087807 1103 5 0.0796282050870758 1103 6 0.12470112225162922 1103 7 0.048068234265272736 1103 8 0.15447761928651005 1103 9 -0.022509456049810694 1103 10-11 0.0890542925088269 1103 12-13 0.27220532583150003 1103 14-15 0.22692748081315273 1103 16-17 0.3181442546818829 1103 18-19 0.28080749177659214 1103 20-21 0.2861065182165703 1103 22-23 0.3733457163288705 1103 24-25 0.2206611070478246 1103 26-27 0.2720615985223134 1103 28-29 0.3306706637800545 1103 30-31 0.3197450694727877 1103 32-33 0.3850047587456018 1103 34-35 0.3033583084079865 1103 36-37 0.3138505890299257 1103 38-39 0.4515280065860665 1103 40-41 0.35378652567858637 1103 42-43 0.3613050002819591 1103 44-45 0.4257772665595141 1103 46-47 0.5785155249971865 1103 48-49 0.7299904544812748 1103 50-51 0.5691773587785534 1103 52-53 0.6530706019789747 1103 54-55 0.7854316593486814 1103 56-57 0.8639296877948155 1103 58-59 0.8400662148910385 1103 60-61 0.7798783631631281 1103 62-63 0.814782781518872 1103 64-65 0.7343452242500419 1103 66-67 0.7084146477729121 1103 68-69 0.7481363521987845 1103 70-71 0.8175830946125373 1103 72-73 0.4213795040545918 1103 74-75 0.41841895454219724 1103 76-77 0.30405086715937557 1103 78-79 0.2627786318202183 1103 80-81 0.325294620312345 1103 82-83 0.28392598585831763 1103 84-85 0.3342054218350725 1103 86-87 0.3491527112577266 1103 88-89 0.28477528044941636 1103 90-91 0.17828396931421864 1103 92-93 0.25173958719783585 1103 94-95 0.4696632046058937 1103 96-97 0.260409364292979 1103 98-99 0.1697756871830336 1103 100-101 0.23130515069489022 1104 1 0.2490558732264212 1104 2 0.16237357974470257 1104 3 0.23064883798949865 1104 4 0.22305930877459446 1104 5 0.2653019371082692 1104 6 0.2735589394133129 1104 7 0.15244437533487343 1104 8 0.3012325286688835 1104 9 0.20505600238185906 1104 10-11 0.2953368547388635 1104 12-13 0.27270155195252954 1104 14-15 0.44249417493970356 1104 16-17 0.4102463831941492 1104 18-19 0.29707357807485124 1104 20-21 0.4409390871260399 1104 22-23 0.39285104874192456 1104 24-25 0.3477976902958133 1104 26-27 0.26169790346063593 1104 28-29 0.363655367881222 1104 30-31 0.27419774569845856 1104 32-33 0.23624156686778974 1104 34-35 0.1322234729446521 1104 36-37 0.23050992687558391 1104 38-39 0.07708740899956723 1104 40-41 0.23456339925920844 1104 42-43 0.3937937565717604 1104 44-45 0.07517890079382994 1104 46-47 0.2392145831973096 1104 48-49 0.468572244123699 1104 50-51 0.22217085746493126 1104 52-53 0.2354655679138702 1104 54-55 0.24820690760089548 1104 56-57 0.37153088118663646 1104 58-59 0.381392694385184 1104 60-61 0.26004017239855415 1104 62-63 0.37192337242593254 1104 64-65 0.3525658792796591 1104 66-67 0.3216716500439105 1104 68-69 0.3909128250134941 1104 70-71 0.47108562166423695 1104 72-73 0.4830139610167805 1104 74-75 0.3655331616936408 1104 76-77 0.4382462182748412 1104 78-79 0.5261976103124475 1104 80-81 0.4299089944495833 1104 82-83 0.49005742594381374 1104 84-85 0.6004442337868028 1104 86-87 0.44767655682926133 1104 88-89 0.41362012838101947 1104 90-91 0.19438028672081842 1104 92-93 0.13279838846247927 1104 94-95 0.19956924726519887 1104 96-97 0.2939406685633621 1104 98-99 0.21177317126371875 1104 100-101 0.1999237885361005 1105 1 -0.010669407764300587 1105 2 0.15696534119693695 1105 3 0.002389297493998299 1105 4 0.07195217577987023 1105 5 0.1985483502776404 1105 6 0.17475983902327386 1105 7 0.08316218109990814 1105 8 0.22290134086225066 1105 9 0.15519551886211502 1105 10-11 0.09411060723072495 1105 12-13 0.042404562513468136 1105 14-15 0.03560888894809722 1105 16-17 0.09141344439043309 1105 18-19 0.08409696546079459 1105 20-21 0.14244526061007434 1105 22-23 0.1463753682985498 1105 24-25 -0.06368353371228608 1105 26-27 -0.07760878687322759 1105 28-29 -0.21890630856968585 1105 30-31 -0.21658393127451347 1105 32-33 -0.12989061864309548 1105 34-35 -0.20456044893160907 1105 36-37 -0.01998294892246122 1105 38-39 0.06072118476478039 1105 40-41 6.898832103487962E-4 1105 42-43 0.056468873453731305 1105 44-45 -0.11209638562367985 1105 46-47 -0.2198317313282061 1105 48-49 -0.1668528518067589 1105 50-51 -0.11025308853648852 1105 52-53 -0.0657928281480693 1105 54-55 -0.05981161491202869 1105 56-57 0.07623885828697752 1105 58-59 0.18530815467965311 1105 60-61 0.006931448462587753 1105 62-63 0.1475353970841553 1105 64-65 0.13442729428313527 1105 66-67 0.07817417590358389 1105 68-69 0.17276737066787717 1105 70-71 0.13461562904192448 1105 72-73 0.039280112573855774 1105 74-75 -0.036672723689896713 1105 76-77 -0.11487995380785065 1105 78-79 0.07793823468132999 1105 80-81 0.08920782624807444 1105 82-83 0.052198244852657893 1105 84-85 0.07853925337131784 1105 86-87 0.05405289274592917 1105 88-89 0.14231037706519345 1105 90-91 -0.03704911491766083 1105 92-93 -0.09545668695610843 1105 94-95 0.06569181568734805 1105 96-97 0.28999594484171354 1105 98-99 -0.04656759241555619 1105 100-101 0.07301874629646932 1106 1 0.1585123269044253 1106 2 0.31742742659348977 1106 3 0.17538377816139672 1106 4 0.3425488462555535 1106 5 0.3597722380088868 1106 6 0.28070451126155405 1106 7 0.22016336861531016 1106 8 0.361213276512359 1106 9 0.3041820230109522 1106 10-11 0.43823427314305263 1106 12-13 0.4211538249842448 1106 14-15 0.48366162508813915 1106 16-17 0.48998823918926604 1106 18-19 0.37478897325807026 1106 20-21 0.3508850222107611 1106 22-23 0.42780438251138975 1106 24-25 0.35575400223059006 1106 26-27 0.19321567743782708 1106 28-29 0.4403505233782141 1106 30-31 0.43298792834472977 1106 32-33 0.33188202817189705 1106 34-35 0.33152340160575733 1106 36-37 0.4341922133339722 1106 38-39 0.22986557618180115 1106 40-41 -0.0036466018934291355 1106 42-43 0.016787934196266008 1106 44-45 0.11966797098943971 1106 46-47 0.31428319626323287 1106 48-49 0.32488121942922277 1106 50-51 0.3611345120102101 1106 52-53 0.25247903819296624 1106 54-55 0.017217243952892147 1106 56-57 0.11900715087882219 1106 58-59 0.12240251360805132 1106 60-61 0.19245552365695318 1106 62-63 0.18615018650555726 1106 64-65 0.12181647818371744 1106 66-67 0.19385703460418569 1106 68-69 0.17042968311140072 1106 70-71 0.21243149693643204 1106 72-73 0.23446955488973487 1106 74-75 0.2784483057888636 1106 76-77 0.48348683907900636 1106 78-79 0.430365172609374 1106 80-81 0.42335804321236736 1106 82-83 0.33721967987913715 1106 84-85 0.5956191231180554 1106 86-87 0.5439057976774784 1106 88-89 0.4799232470434589 1106 90-91 0.3683529598734019 1106 92-93 0.22600429308019088 1106 94-95 0.30060607558144525 1106 96-97 0.3987021505432793 1106 98-99 0.5434231091147694 1106 100-101 0.6029297573276686 1107 1 -0.18640680702180035 1107 2 0.018444975719788204 1107 3 0.0031477968237609844 1107 4 -0.10517902345813468 1107 5 0.15300713112557673 1107 6 -0.019866492028128846 1107 7 -0.2054435959987444 1107 8 -0.017450087966608407 1107 9 -0.12476152709098898 1107 10-11 -0.031026612193478798 1107 12-13 0.011261959123110898 1107 14-15 -0.03207223609489063 1107 16-17 0.01166666364546387 1107 18-19 -0.034124119165660716 1107 20-21 0.18166197444161014 1107 22-23 0.14228866584910094 1107 24-25 0.1992127936799264 1107 26-27 -0.13233750154324753 1107 28-29 0.0373225798656307 1107 30-31 0.1173272406391277 1107 32-33 -0.011587708028336863 1107 34-35 -0.057701647489267316 1107 36-37 -0.08763005641974075 1107 38-39 -0.2046754322362716 1107 40-41 -0.03687330208230577 1107 42-43 0.016600309895160592 1107 44-45 -0.09326215772083657 1107 46-47 -0.023406147927296672 1107 48-49 -0.10056001038552864 1107 50-51 -0.4695064126023425 1107 52-53 -0.4213658215265994 1107 54-55 -0.24237504844081315 1107 56-57 -0.13443731858219365 1107 58-59 -0.18856443949837853 1107 60-61 -0.21791165710029503 1107 62-63 -5.328660167336352E-4 1107 64-65 0.07560773989460046 1107 66-67 -0.18415120835678067 1107 68-69 -0.20864332775830263 1107 70-71 -0.2627364268130563 1107 72-73 -0.21355851370570633 1107 74-75 -0.1981236731471796 1107 76-77 -0.16874995529726888 1107 78-79 -0.15275864692106467 1107 80-81 -0.15263761641213236 1107 82-83 -0.34034952221725234 1107 84-85 -0.20334300178969755 1107 86-87 -0.2201988591576729 1107 88-89 -0.11516474875375593 1107 90-91 -0.34642995881022287 1107 92-93 -0.5352672368083624 1107 94-95 -0.41781519100767284 1107 96-97 -0.4346655805785886 1107 98-99 -0.6119626464482906 1107 100-101 -0.5139117126461912 1108 1 0.056999008841128074 1108 2 0.24243739053123647 1108 3 0.15046312952105012 1108 4 0.22970184912228575 1108 5 0.31262120392021586 1108 6 0.23529896699045594 1108 7 0.10219514757435633 1108 8 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2316 76-77 -1.7914383386239479 2316 78-79 -2.147262960574313 2316 80-81 -2.6234526563761094 2316 82-83 -2.1417833130127484 2316 84-85 -1.9989843187076097 2316 86-87 -2.05471934261433 2316 88-89 -2.1713113208577752 2316 90-91 -1.766046591192815 2316 92-93 -2.2087600166841206 2316 94-95 -1.7231967676658435 2316 96-97 -1.9412716616527526 2316 98-99 -2.1233838493588557 2316 100-101 -2.530020111733311 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1695.0 3 560.0 4 564.0 5 567.0 6 658.0 7 714.0 8 878.0 9 1154.0 10 1421.0 11 1982.0 12 2674.0 13 3470.0 14 3942.0 15 5024.0 16 6765.0 17 9175.0 18 8153.0 19 4582.0 20 4351.0 21 4500.0 22 4710.0 23 5499.0 24 6114.0 25 6933.0 26 8227.0 27 9637.0 28 11689.0 29 14227.0 30 17499.0 31 21982.0 32 28779.0 33 38618.0 34 54606.0 35 80909.0 36 127768.0 37 200999.0 38 201178.0 39 38791.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.433019935903225 20.120327605725127 15.201883573474777 22.244768884896864 2 14.336562149936977 20.800359654884357 42.12000824740515 22.74306994777352 3 18.187557593400953 26.312970113608465 30.954984944673136 24.54448734831745 4 11.667568426568929 17.545519387129247 40.07683206962933 30.710080116672493 5 12.625844813726284 37.9197485443046 35.56543504982753 13.888971592141583 6 29.02953837713819 38.02820195774278 19.103567718826714 13.838691946292315 7 26.329047460628395 33.59529698366253 22.850571243023545 17.22508431268554 8 24.846016852289946 35.05765396985726 21.3336168629223 18.76271231493049 9 25.692563473468923 17.10339446459475 22.09059559395689 35.113446467979436 10-11 23.253595331901526 27.321014681082538 29.26315028617561 20.162239700840324 12-13 24.60343616015853 25.16571111463116 29.34056810075661 20.8902846244537 14-15 21.858415169104823 26.228705781313277 27.946433221691592 23.96644582789031 16-17 20.48111452245419 29.54798257469185 28.62261516737341 21.348287735480547 18-19 20.271365473948926 27.969956458867102 31.479340946787133 20.27933712039684 20-21 21.10606787693658 27.152422054397846 31.367269144494237 20.374240924171332 22-23 20.76069663215106 27.024041184916577 31.301737541807174 20.91352464112519 24-25 20.689521416896746 27.258163245786864 31.367204187947532 20.685111149368854 26-27 20.469307250553666 27.353921981203055 31.36633850653132 20.81043226171196 28-29 20.248985629844693 27.355156018525406 31.549674912697217 20.846183438932687 30-31 20.45740506947898 27.269012671572067 31.412831401354296 20.860750857594653 32-33 20.293952616466278 27.37215223772107 31.342972703564925 20.99092244224773 34-35 20.787318651142822 27.4070567181436 31.026116785901014 20.77950784481256 36-37 20.78407743218945 27.066834997513293 31.09678598603194 21.05230158426532 38-39 20.42318629888927 27.576025181614533 31.09003787444049 20.910750645055707 40-41 20.653631653000414 27.4087570297257 31.202045899911155 20.735565417362732 42-43 20.47356890808589 27.38326690602107 30.85828054837203 21.284883637521006 44-45 20.669848730637028 27.558133418207266 30.660247278570164 21.11177057258554 46-47 20.83115480911877 27.812952973632417 30.186215999081835 21.16967621816698 48-49 20.76469834967856 27.87103273809682 30.09367177524725 21.270597136977372 50-51 20.795705003815083 27.910408266932485 29.961200932622884 21.33268579662955 52-53 21.02562222402881 28.19050382067153 29.60729522172966 21.176578733569997 54-55 20.811917331087454 28.007587813801 29.719787562467953 21.460707292643594 56-57 20.820913048976568 28.320958213646957 29.455919813050148 21.40220892432633 58-59 20.974296411146902 27.977931916892402 29.565184586135768 21.482587085824928 60-61 21.03355855903726 27.984701088862487 29.89276096673475 21.0889793853655 62-63 20.75034179627826 27.965976060179525 30.077275449276797 21.20640669426542 64-65 20.742810657034294 27.952477886404104 29.87061015830609 21.434101298255513 66-67 20.69433698369242 28.196579488836367 29.407160287040657 21.701923240430563 68-69 20.684714865106155 28.023207863210075 29.765232418736343 21.526844852947427 70-71 20.784523815218208 27.89568594812041 29.679375875940515 21.640414360720875 72-73 20.832762045666875 27.7255204563499 29.7119541014203 21.72976339656292 74-75 20.677318467224854 28.183250650347958 29.500474308930556 21.638956573496625 76-77 20.731664537087628 27.990588040199082 29.61020199464664 21.66754542806665 78-79 20.78024157547001 27.916379684191728 29.464065542545683 21.839313197792574 80-81 20.675025184531513 28.16855960427725 29.317559000911693 21.83885621027954 82-83 20.875588210749672 28.1024545789413 29.3244552688747 21.69750194143433 84-85 20.73626602553883 27.981478292848323 29.30826855093629 21.973987130676566 86-87 20.63768143667771 28.042030283996162 29.457122116967604 21.863166162358525 88-89 20.817258127942694 27.810849302256376 29.256395540392603 22.115497029408328 90-91 20.527983766773865 27.81980821365953 29.65417764816991 21.9980303713967 92-93 20.809891289153416 27.868841131638828 29.547313641396123 21.773953937811637 94-95 20.588590088665814 27.666656924143833 29.466703156843106 22.27804983034725 96-97 20.56884359511533 27.678591357973513 29.4853112334865 22.26725381342466 98-99 20.351044853546167 27.795825688365973 29.39228621907376 22.460843239014103 100-101 20.405696333742647 27.646355271341317 29.49054066265861 22.45740773225743 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 149.0 1 109.5 2 98.5 3 142.5 4 133.0 5 130.5 6 231.5 7 499.5 8 839.5 9 1069.5 10 1049.0 11 940.0 12 998.0 13 1105.5 14 1161.0 15 1217.5 16 1316.0 17 1423.5 18 1532.0 19 1645.5 20 1867.0 21 2251.0 22 2695.0 23 3312.5 24 4106.0 25 5151.0 26 6843.0 27 8826.5 28 10240.0 29 11747.5 30 14128.0 31 16926.5 32 19778.5 33 22755.0 34 26112.5 35 29144.5 36 33061.0 37 36622.5 38 37272.0 39 36727.5 40 35837.5 41 35251.5 42 34761.5 43 34907.0 44 35515.5 45 35512.0 46 34186.5 47 33367.0 48 35156.0 49 34034.0 50 30131.0 51 27543.5 52 25383.0 53 23971.5 54 22432.0 55 19967.5 56 17684.5 57 15364.5 58 13487.5 59 11774.0 60 9654.5 61 7748.5 62 6053.0 63 4436.5 64 3183.0 65 2379.5 66 1737.0 67 1282.5 68 891.5 69 587.5 70 412.0 71 296.5 72 230.0 73 171.5 74 118.0 75 83.0 76 61.5 77 41.5 78 26.0 79 15.0 80 7.5 81 4.5 82 4.0 83 3.5 84 4.0 85 2.5 86 1.0 87 3.5 88 4.0 89 2.0 90 2.0 91 1.5 92 0.5 93 1.5 94 1.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0253984042575802 2 0.010839486335871049 3 0.014983995817233507 4 0.026673637944153266 5 0.059510905373409674 6 0.045802143242749234 7 0.017109385294855284 8 0.050903077989041486 9 0.031455764268802255 10-11 0.02502646109899639 12-13 0.03836328007107303 14-15 0.020935086354574476 16-17 0.020031795826585222 18-19 0.018012675822844534 20-21 0.014824591606411874 22-23 0.008182749488843831 24-25 0.0019128505298595967 26-27 0.0 28-29 0.002763006320908306 30-31 0.0 32-33 2.125389477621774E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 5.313473694054435E-5 44-45 5.313473694054435E-5 46-47 0.0 48-49 5.844821063459879E-4 50-51 2.125389477621774E-4 52-53 4.782126324648992E-4 54-55 0.0022847936884434076 56-57 3.1880842164326616E-4 58-59 7.43886317167621E-4 60-61 0.0023379284253839516 62-63 0.00207225474068123 64-65 0.0011158294757514313 66-67 0.009776791597060162 68-69 0.012327258970206291 70-71 0.02438884425570986 72-73 0.01562161266052004 74-75 0.017162520031795827 76-77 0.030393069529991372 78-79 0.019128505298595968 80-81 0.04744932008790611 82-83 0.035440869539343084 84-85 0.017215654768736374 86-87 0.011795911600800848 88-89 0.012114720022444112 90-91 0.023113610569136793 92-93 0.03161516847962389 94-95 0.01184904633774139 96-97 0.019447313720239234 98-99 0.031668303216564435 100-101 0.04240152007855439 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 941004.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.78549568985916 #Duplication Level Percentage of deduplicated Percentage of total 1 83.20607792441379 51.40928768968954 2 10.199523425310776 12.60365221266313 3 2.792638610749323 5.176336825433597 4 1.1580803630965468 2.862102771304489 5 0.5882567628944313 1.817286784417219 6 0.36806625295338574 1.3644693531260415 7 0.2525551318227923 1.0922970806082264 8 0.19169250422251055 0.9475053114734588 9 0.1437160226430917 0.7991609127820598 >10 0.9483856239605465 11.445195765515786 >50 0.10251755124617143 4.367412380004172 >100 0.0471093334743417 4.695886523560311 >500 8.628082576311552E-4 0.3803066619896404 >1k 5.176849545786931E-4 1.039099727432306 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4424 0.47013615244993645 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3194 0.33942469957619736 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2128 0.22614144041895679 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.0626947388108872E-4 0.0 5 0.0 0.0 0.0 1.0626947388108872E-4 0.0 6 0.0 0.0 0.0 1.0626947388108872E-4 0.0 7 0.0 0.0 0.0 1.0626947388108872E-4 0.0 8 0.0 0.0 0.0 1.0626947388108872E-4 0.0 9 0.0 0.0 0.0 1.0626947388108872E-4 0.0 10-11 0.0 0.0 0.0 1.5940421082163308E-4 0.0 12-13 0.0 0.0 0.0 2.1253894776217743E-4 0.0 14-15 0.0 0.0 0.0 2.1253894776217743E-4 0.0 16-17 0.0 0.0 0.0 3.188084216432661E-4 0.0 18-19 0.0 0.0 0.0 4.250778955243548E-4 0.0 20-21 0.0 0.0 0.0 5.313473694054436E-4 0.0 22-23 0.0 0.0 0.0 9.564252649297984E-4 0.0 24-25 0.0 0.0 0.0 0.0019128505298595967 0.0 26-27 0.0 0.0 0.0 0.0025504673731461293 0.0 28-29 0.0 0.0 0.0 0.004569587376886814 0.0 30-31 0.0 0.0 0.0 0.008395288436606009 0.0 32-33 0.0 0.0 0.0 0.0126992021287901 0.0 34-35 0.0 0.0 0.0 0.015409073712757862 0.0 36-37 0.0 0.0 0.0 0.019606717931060866 0.0 38-39 0.0 0.0 0.0 0.02672677268109381 0.0 40-41 0.0 0.0 0.0 0.04256092428937602 0.0 42-43 0.0 0.0 0.0 0.059670309584231314 0.0 44-45 0.0 0.0 0.0 0.08033972225410306 0.0 46-47 0.0 0.0 0.0 0.11934061916846261 0.0 48-49 0.0 0.0 0.0 0.140169436049156 0.0 50-51 0.0 0.0 0.0 0.16631172662390384 0.0 52-53 0.0 0.0 0.0 0.19436686772851125 0.0 54-55 0.0 0.0 0.0 0.23559942359437364 0.0 56-57 0.0 0.0 0.0 0.3524427101266307 0.0 58-59 0.0 0.0 0.0 0.46009368716817356 0.0 60-61 0.0 0.0 0.0 0.5336852978308275 0.0 62-63 0.0 0.0 0.0 0.6296997674823912 0.0 64-65 0.0 0.0 0.0 0.7227386918652843 0.0 66-67 0.0 0.0 0.0 0.8762980816234576 0.0 68-69 0.0 0.0 0.0 1.0596129240683356 0.0 70-71 0.0 0.0 0.0 1.1575402442497587 0.0 72-73 0.0 0.0 0.0 1.268644979192437 0.0 74-75 0.0 0.0 0.0 1.4680065121933594 0.0 76-77 0.0 0.0 0.0 1.712373167382923 0.0 78-79 0.0 0.0 0.0 1.9331479993708847 0.0 80-81 0.0 0.0 0.0 2.0892047217652636 0.0 82-83 0.0 0.0 0.0 2.3410633748634435 0.0 84-85 0.0 0.0 0.0 2.7321881734827906 0.0 86-87 0.0 0.0 0.0 3.105034622594591 0.0 88-89 0.0 0.0 0.0 3.530856404436113 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5290 0.0 32.607872 1 GGTATCA 3490 0.0 32.26966 1 ATCAACG 6435 0.0 26.365582 3 CAACGCA 6480 0.0 26.182486 5 TATCAAC 6530 0.0 26.054789 2 TCAACGC 6555 0.0 25.882915 4 AACGCAG 6630 0.0 25.663168 6 GTACATG 4080 0.0 24.807953 1 TACATGG 4130 0.0 23.704723 2 ACATGGG 4000 0.0 23.049389 3 GTATAGG 330 0.0 23.039738 1 ACGCAGA 7370 0.0 22.891727 7 CGCAGAG 7320 0.0 22.85332 8 GAGTACT 3815 0.0 21.981834 12-13 GGGCGTA 130 2.9338783E-4 21.930893 9 TCGCGGG 65 2.9396132E-4 21.92623 86-87 CATGGGG 1570 0.0 21.491978 4 GCAGAGT 7785 0.0 21.240711 9 AGTACTT 4090 0.0 20.620008 12-13 AGAGTAC 6220 0.0 20.206156 10-11 >>END_MODULE