FastQCFastQC Report
Thu 26 May 2016
SRR936917_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936917_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences941004
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53520.5687542242115867No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42060.4469694071438591No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28030.29787333528869164No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14850.15781016871341674No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11260.11965942759010588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA65100.029.9981441
CGCGGAA705.8038495E-727.14583482-83
GGTATCA44500.027.12111
ATACGTC1301.0022422E-525.5756515
ATCAACG79550.024.240123
GTACATG40100.023.9353921
TCAACGC81150.023.7621864
ATTCGCG901.9503386E-723.75260580-81
CAACGCA81200.023.7488175
TATCAAC82000.023.5158732
AACGCAG82850.023.2758466
AGTACTT50050.023.05956512-13
ACATGGG40050.022.6506023
CCCGCGA953.2807293E-722.50605420-21
GTGTAGC4100.022.019291
GAGTACT48750.022.01824612-13
TACATGG43100.021.9292962
GTGTACG1100.002871607721.5978681
AGAGTAC74800.021.20883810-11
GTACTTT51300.020.8311914-15
Produced by FastQC (version 0.11.5)