##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936917_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 941004 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.44346251450578 33.0 31.0 34.0 31.0 34.0 2 32.57576800948774 34.0 31.0 34.0 31.0 34.0 3 32.67587810466268 34.0 31.0 34.0 31.0 34.0 4 36.26114022894696 37.0 35.0 37.0 35.0 37.0 5 36.196382799648035 37.0 35.0 37.0 35.0 37.0 6 36.248112654143874 37.0 37.0 37.0 35.0 37.0 7 36.21124139748609 37.0 36.0 37.0 35.0 37.0 8 36.18625531878717 37.0 36.0 37.0 35.0 37.0 9 37.9172809042257 39.0 38.0 39.0 35.0 39.0 10-11 37.83734181788813 39.0 38.0 39.0 35.0 39.0 12-13 37.759250226353984 39.0 37.0 39.0 35.0 39.0 14-15 39.02714122362923 40.0 38.0 41.0 36.0 41.0 16-17 38.95084133542471 40.0 38.0 41.0 36.0 41.0 18-19 39.11315254770437 40.0 38.5 41.0 36.0 41.0 20-21 39.14617312997606 40.0 39.0 41.0 36.0 41.0 22-23 39.07062297290979 40.0 38.5 41.0 36.0 41.0 24-25 38.96371269410119 40.0 38.0 41.0 35.0 41.0 26-27 38.80609806121972 40.0 38.0 41.0 35.0 41.0 28-29 38.734602084581994 40.0 38.0 41.0 35.0 41.0 30-31 38.71082375845374 40.0 38.0 41.0 35.0 41.0 32-33 38.543215012901115 40.0 38.0 41.0 35.0 41.0 34-35 38.38147871847516 40.0 38.0 41.0 34.0 41.0 36-37 38.2393624256645 40.0 38.0 41.0 34.0 41.0 38-39 38.077952378523364 40.0 38.0 41.0 34.0 41.0 40-41 37.91627825173963 40.0 38.0 41.0 33.5 41.0 42-43 37.66411566794615 40.0 37.0 41.0 33.0 41.0 44-45 37.35723865148289 40.0 37.0 41.0 33.0 41.0 46-47 36.94628184364785 40.0 37.0 41.0 31.5 41.0 48-49 36.540245843800875 40.0 37.0 41.0 31.0 41.0 50-51 36.2485132900604 39.5 36.0 41.0 30.0 41.0 52-53 36.03259391033407 39.0 36.0 40.5 30.0 41.0 54-55 35.74609512818224 39.0 35.0 40.0 28.5 41.0 56-57 35.48627476610089 39.0 35.0 40.0 28.0 41.0 58-59 35.175160785713985 38.0 35.0 40.0 27.5 41.0 60-61 34.863310357873075 38.0 34.0 40.0 26.5 41.0 62-63 34.5372756120059 38.0 34.0 40.0 26.0 41.0 64-65 34.184280831962454 37.0 34.0 40.0 26.0 41.0 66-67 33.80488393248063 37.0 34.0 39.0 25.0 40.5 68-69 33.50051646964306 36.0 33.0 39.0 25.0 40.0 70-71 33.04438344576644 36.0 33.0 39.0 23.5 40.0 72-73 32.52794674624125 35.0 32.0 38.0 22.0 40.0 74-75 32.03383088701004 35.0 31.5 37.5 20.5 39.0 76-77 30.62143678454077 33.5 30.0 36.0 20.0 38.5 78-79 31.326236126520186 34.5 31.0 36.0 20.0 39.0 80-81 31.016898440389202 35.0 31.0 36.0 17.5 38.0 82-83 30.71109633965424 34.0 31.0 35.5 16.5 37.0 84-85 30.372626471300865 34.0 31.0 35.0 13.0 37.0 86-87 30.053158647572168 34.0 30.5 35.0 9.0 36.0 88-89 29.671871214149995 34.0 30.0 35.0 6.0 36.0 90-91 29.25454780213474 34.0 29.5 35.0 2.0 35.5 92-93 28.81178985424079 34.0 29.0 35.0 2.0 35.0 94-95 28.25194632541413 34.0 29.0 35.0 2.0 35.0 96-97 27.600278000943675 33.0 27.5 35.0 2.0 35.0 98-99 26.847443794075264 33.0 26.5 34.5 2.0 35.0 100-101 23.30781006244394 29.0 13.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.019574809343033905 1101 2 -0.0061995383903550305 1101 3 0.010969694935354823 1101 4 0.0903235927660333 1101 5 0.18405452574394587 1101 6 0.06801966070166543 1101 7 0.10301282687061786 1101 8 0.15686150551447042 1101 9 0.11134881837416089 1101 10-11 0.160425387432376 1101 12-13 0.09745794370306271 1101 14-15 0.1378431550361512 1101 16-17 -0.0060105814960564885 1101 18-19 0.13898269379348704 1101 20-21 0.15670908908639802 1101 22-23 0.09766333403296557 1101 24-25 0.05716809855420735 1101 26-27 0.08504046272172872 1101 28-29 0.04801593833189344 1101 30-31 0.08961136072030484 1101 32-33 0.22751759869672838 1101 34-35 0.22859792645137844 1101 36-37 0.14543472667306645 1101 38-39 0.05327558897708684 1101 40-41 0.1337947970627127 1101 42-43 0.12160037088315079 1101 44-45 0.06331585232809545 1101 46-47 0.030040500973413486 1101 48-49 0.11672809778139026 1101 50-51 0.0626051246922188 1101 52-53 0.10528628208130186 1101 54-55 0.15437386106393802 1101 56-57 0.04308177380693934 1101 58-59 0.10068276840799939 1101 60-61 0.14109151342212556 1101 62-63 0.09838594184635241 1101 64-65 0.1386340247137383 1101 66-67 0.05874954721782899 1101 68-69 0.08315794710547664 1101 70-71 -0.06390325144642617 1101 72-73 -0.022344148463446345 1101 74-75 -0.007255218727877377 1101 76-77 -0.03416346183706409 1101 78-79 -0.11176089883892004 1101 80-81 0.23360595733044676 1101 82-83 0.22369609064196894 1101 84-85 0.10382710815174434 1101 86-87 -0.3081737197933201 1101 88-89 -0.214335598664249 1101 90-91 -0.1193034267150388 1101 92-93 0.004887489433713199 1101 94-95 -0.2859622903151191 1101 96-97 -0.1357166378551753 1101 98-99 -0.05595999736066659 1101 100-101 0.31308287658030665 1102 1 0.1301271810651201 1102 2 0.11865852378097941 1102 3 0.10872918192635694 1102 4 0.1317463385469111 1102 5 0.07897638550424091 1102 6 0.04689941446392254 1102 7 0.04030094680269514 1102 8 0.04363002519274772 1102 9 0.07403174620506547 1102 10-11 0.08968670498503428 1102 12-13 0.1062894026699368 1102 14-15 0.15882043677016355 1102 16-17 0.17635950817374635 1102 18-19 0.0384830110360852 1102 20-21 0.15283595737835753 1102 22-23 0.1713678240937213 1102 24-25 0.23087253728121482 1102 26-27 0.15179564589082872 1102 28-29 0.19145912932015108 1102 30-31 0.13332025521712154 1102 32-33 0.33020127600043736 1102 34-35 0.28976594388021937 1102 36-37 0.23704930669109103 1102 38-39 0.28792448663605796 1102 40-41 0.10551220885592016 1102 42-43 0.07279121129883492 1102 44-45 0.11349228643916831 1102 46-47 -0.023663845352835722 1102 48-49 -0.019054043821746802 1102 50-51 0.13796846500009252 1102 52-53 0.18632841887959017 1102 54-55 0.038415788420515184 1102 56-57 -0.09669849719421109 1102 58-59 -0.04612791681547179 1102 60-61 -0.15466508450521843 1102 62-63 -0.12154813534728959 1102 64-65 -0.15403664461680933 1102 66-67 -0.0450193256366731 1102 68-69 -0.13743833550942952 1102 70-71 -0.3721365181827636 1102 72-73 -0.1391191977951891 1102 74-75 -0.019699803940888216 1102 76-77 -0.1428254680833554 1102 78-79 -0.14766783279701912 1102 80-81 0.152082309923518 1102 82-83 0.05652053536475776 1102 84-85 0.12341170515097843 1102 86-87 -0.020771433593271382 1102 88-89 0.014982288684855405 1102 90-91 0.3411618829609644 1102 92-93 0.21721518768980275 1102 94-95 -0.08456714156016432 1102 96-97 0.08903947548154179 1102 98-99 0.31229064071789026 1102 100-101 0.25281396984552273 1103 1 0.1317041569948998 1103 2 0.07980983811228981 1103 3 0.07733650051317653 1103 4 0.039570266912527075 1103 5 0.014908829318237338 1103 6 0.02842397229006366 1103 7 -0.12124987548553179 1103 8 0.016963284645740373 1103 9 0.10720752970610903 1103 10-11 0.05428758489991736 1103 12-13 0.03276608603356124 1103 14-15 0.1487102811781682 1103 16-17 0.13675668889983683 1103 18-19 -0.007427989008242264 1103 20-21 0.1387174237554163 1103 22-23 0.1500805884006482 1103 24-25 -0.06071036754612891 1103 26-27 0.0785640642604406 1103 28-29 0.03821568989464197 1103 30-31 0.007298490067960017 1103 32-33 -0.06523264172641063 1103 34-35 -0.0163000940647251 1103 36-37 -0.020137680764030108 1103 38-39 -0.031554712353255354 1103 40-41 -0.01546389824103045 1103 42-43 0.21120908221509893 1103 44-45 0.08478063033739147 1103 46-47 0.10908361685736878 1103 48-49 0.3602186411376991 1103 50-51 0.3833616561876454 1103 52-53 0.40004605928918835 1103 54-55 0.24809180464463765 1103 56-57 0.10401601807685523 1103 58-59 0.19614678507733885 1103 60-61 0.16829138519645426 1103 62-63 0.34854722570594276 1103 64-65 0.2838786552635071 1103 66-67 0.11982203479597331 1103 68-69 0.170363428753177 1103 70-71 0.010863337750564028 1103 72-73 -0.11442921818616014 1103 74-75 0.11557796768020268 1103 76-77 -0.08359245686831684 1103 78-79 0.1906273043987774 1103 80-81 0.28116466064828316 1103 82-83 0.1991058117511244 1103 84-85 0.09473911328077023 1103 86-87 -0.01460704244118105 1103 88-89 -0.2917217983757432 1103 90-91 -0.11952481636081203 1103 92-93 -0.06475708501732313 1103 94-95 -0.5249008221311158 1103 96-97 -0.5389934049652894 1103 98-99 -0.7060371331196045 1103 100-101 -0.16504863487986654 1104 1 -0.013516709550046357 1104 2 0.054290512289604465 1104 3 -0.015048390612086848 1104 4 0.008542079710316841 1104 5 -0.01755766219888244 1104 6 -0.08961884286779309 1104 7 0.035305986829115454 1104 8 0.04297855827979902 1104 9 -0.004850514556260066 1104 10-11 0.0025478712248983015 1104 12-13 0.07941468112640848 1104 14-15 -0.036210901526558814 1104 16-17 -0.05148305817234444 1104 18-19 0.0023793650461669813 1104 20-21 0.019355977435779437 1104 22-23 0.09700276188775803 1104 24-25 0.008837267935014381 1104 26-27 -0.07966193431462187 1104 28-29 -0.11276479503210624 1104 30-31 0.03155707313782585 1104 32-33 -0.014194825010022782 1104 34-35 -0.11154851326586623 1104 36-37 -0.0070117623421523945 1104 38-39 0.04038360309404254 1104 40-41 0.12967124892912096 1104 42-43 -0.006143246680125003 1104 44-45 -0.11576958785737901 1104 46-47 0.12549498079734178 1104 48-49 0.00550574538136317 1104 50-51 0.10204736665654224 1104 52-53 0.13489961274690643 1104 54-55 0.022600031478141602 1104 56-57 0.12355864398414695 1104 58-59 0.1182456851975715 1104 60-61 0.05513179130590373 1104 62-63 0.13644103387266426 1104 64-65 -0.024529357828185994 1104 66-67 0.04665581713555156 1104 68-69 0.003796211404463179 1104 70-71 -0.10641217767892641 1104 72-73 0.05259804502797749 1104 74-75 0.01557267979668353 1104 76-77 -0.18536306291542815 1104 78-79 0.019687285696370793 1104 80-81 0.04220659634762214 1104 82-83 0.017787347575140444 1104 84-85 0.09932454321353035 1104 86-87 -0.08640815122685908 1104 88-89 -0.15298721673082127 1104 90-91 0.0630026249715101 1104 92-93 -0.07295998389072622 1104 94-95 -0.12192123892765849 1104 96-97 -0.04245529880684984 1104 98-99 0.14291837073220037 1104 100-101 0.34534623086355154 1105 1 0.09359430325304174 1105 2 0.04594494006595795 1105 3 0.049172467204748216 1105 4 0.043968686897585485 1105 5 0.023118501571893546 1105 6 0.01715969343877788 1105 7 0.029921791003204135 1105 8 0.08784777274225064 1105 9 0.07436750621940291 1105 10-11 0.11474117196087974 1105 12-13 0.06994047484534605 1105 14-15 0.08521343053227781 1105 16-17 -0.06048362283284092 1105 18-19 -0.06472421512119553 1105 20-21 0.060036949750930546 1105 22-23 0.031069031872412722 1105 24-25 0.041832469007729856 1105 26-27 0.1607975701854869 1105 28-29 -0.05966850461800988 1105 30-31 -0.00900608984023421 1105 32-33 -0.06727772019337408 1105 34-35 0.04873607012871872 1105 36-37 0.04995746709547433 1105 38-39 0.03741947895666442 1105 40-41 -0.03307359184623948 1105 42-43 0.10594392261223362 1105 44-45 0.021105627668440263 1105 46-47 -0.011227048243512172 1105 48-49 -0.11036025285184081 1105 50-51 -0.08135223299779426 1105 52-53 -0.16499143937633676 1105 54-55 -0.006704955257148981 1105 56-57 -0.058823605068226925 1105 58-59 -0.08069478639698247 1105 60-61 -0.2551072483041352 1105 62-63 -0.013663861088119234 1105 64-65 -0.13455640932494362 1105 66-67 4.5017080536524645E-4 1105 68-69 0.041923159723602055 1105 70-71 -0.2711206218950011 1105 72-73 -0.05092235899965658 1105 74-75 -0.0813507448211368 1105 76-77 -0.4833028764263396 1105 78-79 -0.24126657838119314 1105 80-81 0.044352581006993574 1105 82-83 0.15657018134651324 1105 84-85 -0.0015590611614193506 1105 86-87 -0.2757432119725145 1105 88-89 -0.20714963012550314 1105 90-91 -0.09560240256771024 1105 92-93 9.696684789943788E-4 1105 94-95 -0.12465661373995118 1105 96-97 -0.29246903750505737 1105 98-99 -0.11945057926030955 1105 100-101 0.1024663112074613 1106 1 0.22383076072281227 1106 2 0.16483465050637847 1106 3 0.11968399506655203 1106 4 0.0411679430200067 1106 5 0.11342008567263662 1106 6 0.07383367938277985 1106 7 0.1389958277340284 1106 8 0.09138231151574416 1106 9 0.21493870859741548 1106 10-11 0.1615845468945949 1106 12-13 0.20993565756587884 1106 14-15 0.24688263654006448 1106 16-17 0.17457440858029827 1106 18-19 0.06291968709923168 1106 20-21 0.2788469323489764 1106 22-23 0.21948720907361263 1106 24-25 0.25915619018807234 1106 26-27 0.25455807297503696 1106 28-29 0.3962207128914983 1106 30-31 0.38128783889391116 1106 32-33 0.3352984040252167 1106 34-35 0.3495359625801555 1106 36-37 0.3538773882265289 1106 38-39 0.36945139996821297 1106 40-41 0.37548865562002476 1106 42-43 0.3126388207771882 1106 44-45 0.041475437890035494 1106 46-47 0.14219303346351353 1106 48-49 0.14314347773900238 1106 50-51 0.1299302836386289 1106 52-53 0.20471048678858494 1106 54-55 0.11568288428637175 1106 56-57 0.13893038505821664 1106 58-59 0.09195893709864578 1106 60-61 -0.03835954678127962 1106 62-63 0.16631380764736292 1106 64-65 -0.15590741348498227 1106 66-67 -0.01895338277847003 1106 68-69 -0.023215607799720317 1106 70-71 -0.27804219359777704 1106 72-73 -0.18374203590585125 1106 74-75 0.023117001812373417 1106 76-77 0.17109466489396752 1106 78-79 0.0490090521596116 1106 80-81 0.10333998139386935 1106 82-83 0.23654075486416914 1106 84-85 0.11590226990144359 1106 86-87 0.015636845237349206 1106 88-89 -0.1205011496224131 1106 90-91 0.06609761162272676 1106 92-93 0.13806608128623665 1106 94-95 0.044909756421294134 1106 96-97 0.3443323636137485 1106 98-99 0.15180146528271976 1106 100-101 0.28050026369190917 1107 1 0.008509985709366674 1107 2 -0.04045135146992607 1107 3 -0.05092278192320521 1107 4 0.009496960994418657 1107 5 0.03721647578107934 1107 6 0.08745833071428422 1107 7 -0.019043248153749914 1107 8 0.06345443429709263 1107 9 -0.04707237374463347 1107 10-11 -0.08821532051756265 1107 12-13 -0.04594167366605717 1107 14-15 -0.08085792908467226 1107 16-17 -0.09064661284639897 1107 18-19 0.007819120473847363 1107 20-21 -0.145238711890336 1107 22-23 0.06357923253916198 1107 24-25 -0.0882630375502984 1107 26-27 -0.03916307978867195 1107 28-29 -0.17482479225566294 1107 30-31 -0.13887082872339107 1107 32-33 -0.04434529742699311 1107 34-35 -0.0801049245075447 1107 36-37 -0.0774708800011652 1107 38-39 -0.11152036882860727 1107 40-41 -0.15727211088778148 1107 42-43 -0.0684824220461806 1107 44-45 -0.1206647925125992 1107 46-47 -0.16412396404733443 1107 48-49 -0.298894685043841 1107 50-51 -0.3168782269996697 1107 52-53 -0.15692284529392708 1107 54-55 0.0233044961042026 1107 56-57 -0.12923946496741934 1107 58-59 -0.10932284958503402 1107 60-61 -0.11546062624642417 1107 62-63 0.08822144645807839 1107 64-65 -0.1950864946262385 1107 66-67 -0.178931243110938 1107 68-69 -0.1294875754783078 1107 70-71 -0.2141780085751961 1107 72-73 -0.3443062221792559 1107 74-75 -0.34606195424601793 1107 76-77 -0.11029175360829058 1107 78-79 0.03468237323482626 1107 80-81 0.353436920616101 1107 82-83 0.04577687379296336 1107 84-85 0.13430195969593584 1107 86-87 -0.05202360481080959 1107 88-89 -0.12531214940977264 1107 90-91 -0.033956174010256746 1107 92-93 0.058269466028630035 1107 94-95 0.09067445053114298 1107 96-97 -0.08785395982553013 1107 98-99 -0.2192631150017199 1107 100-101 -0.2362201886077422 1108 1 0.03129747156693696 1108 2 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92-93 -1.096221236334415 2316 94-95 -1.008588185965536 2316 96-97 -0.7573324324954918 2316 98-99 -0.7847866379720507 2316 100-101 -0.9766078545273196 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 3.0 5 17.0 6 35.0 7 68.0 8 173.0 9 349.0 10 666.0 11 1169.0 12 1840.0 13 2849.0 14 3609.0 15 5210.0 16 7476.0 17 11306.0 18 10132.0 19 5394.0 20 4119.0 21 3981.0 22 4054.0 23 4451.0 24 5081.0 25 5905.0 26 7050.0 27 8843.0 28 11105.0 29 14049.0 30 17716.0 31 22803.0 32 29825.0 33 41299.0 34 58612.0 35 87367.0 36 142766.0 37 231061.0 38 178281.0 39 12336.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.4199742965296 20.87508729931787 15.45737401525838 21.247564388894144 2 15.090732287941504 21.896477514865218 40.881232364990886 22.13155783220239 3 18.847462598379174 26.780814493486734 30.96008297538156 23.411639932752536 4 12.372476089266737 18.585441020191283 39.249840595111586 29.792242295430395 5 12.867214166996103 38.59314094307782 34.754581277019014 13.785063612907065 6 29.44929033245342 38.802598076097446 17.979200938571992 13.768910652877139 7 26.608919834559686 34.350651006796994 22.109682849382146 16.93074630926117 8 25.385970729136115 35.585608562769124 20.49534327165453 18.53307743644023 9 26.48160900485014 17.345941143714587 21.44029143340517 34.7321584180301 10-11 23.78105259577833 28.041250590535384 28.284054295776258 19.893642517910028 12-13 25.287193253163643 25.63347233380517 28.84265104080323 20.23668337222796 14-15 22.39496325201593 26.743031910597615 27.522890444673987 23.339114392712464 16-17 20.744757726853447 30.004707737692932 28.14887078057054 21.10166375488308 18-19 20.50297555791711 28.192507970244417 31.518597236981932 19.785919234856536 20-21 21.324027556011337 27.357763212777996 31.655193851173095 19.663015380037567 22-23 21.069763709990664 27.24341075056045 31.398690837830845 20.28813470161804 24-25 21.0682465774681 27.440941994713558 31.56498532783078 19.925826099987564 26-27 20.886087720314382 27.5955902318545 31.49316679357654 20.02515525425458 28-29 20.43208151302387 27.644598370037077 31.763607554917467 20.159712562021586 30-31 20.797574862310736 27.563039498819048 31.412493922543312 20.226891716326907 32-33 20.76148543487606 27.74937603849671 31.261255085877398 20.227883440749828 34-35 21.121748685446608 27.5672048152824 31.313469443275483 19.99757705599551 36-37 21.273745427337985 27.24435072981918 31.246824909290716 20.235078933552128 38-39 20.671726420438542 27.932412856351814 31.416670829396452 19.979189893813192 40-41 20.965763935234584 27.750543041628234 31.29168455551352 19.992008467623666 42-43 20.97546875464929 27.944727121244966 30.74705314748928 20.332750976616467 44-45 21.14268377180118 27.868213100050582 30.541368580792433 20.447734547355804 46-47 21.402398719662656 28.33681543716167 29.837609271818767 20.423176571356915 48-49 21.391998537691638 28.472610411720574 29.654093932057922 20.48129711852987 50-51 21.36460783240536 28.612648880849623 29.373964463083073 20.648778823661946 52-53 21.584392839987927 28.73547827639415 29.21895124781616 20.46117763580176 54-55 21.339506422123648 28.66224709500662 29.16423122620796 20.83401525666177 56-57 21.396271932155116 28.8873184054752 29.047897382488287 20.668512279881398 58-59 21.51397449521785 28.4721041445271 29.12003188097768 20.893889479277362 60-61 21.398872132708526 28.799458436660945 29.36753669978995 20.434132730840577 62-63 21.24710800978999 28.464994829753838 29.75277402570351 20.535123134752663 64-65 21.122705110711536 28.631546730938446 29.35045972174401 20.89528843660601 66-67 21.18324707482704 28.83636035155212 28.94258052860346 21.037812045017375 68-69 21.237455439478722 28.635684230158382 29.20454986235322 20.922310468009677 70-71 21.350227471500972 28.68951760738876 29.055246722359367 20.9050081987509 72-73 21.33859154690097 28.5415896212981 29.181863201431664 20.937955630369267 74-75 21.347836991128624 28.959069249439963 28.614224806695827 21.07886895273559 76-77 21.347943260602506 28.75806053959388 28.812736183905702 21.08126001589791 78-79 21.45924251932438 28.74004555915171 28.577554439573017 21.223157481950892 80-81 21.513187399439303 29.00388321037563 28.33957155032977 21.143357839855298 82-83 21.549183350923677 28.9213982571209 28.37503467583278 21.15438371612264 84-85 21.30983417170344 28.85107337235217 28.504813941352708 21.334278514591677 86-87 21.199730268726956 28.960774717528757 28.540129966262334 21.29936504748195 88-89 21.177744574400478 28.851054514230572 28.426932142545922 21.54426876882302 90-91 20.992099316202168 28.808723111559097 28.734865081570614 21.464312490668117 92-93 21.254121940359255 28.724252152760734 28.524199230394014 21.497426676485997 94-95 21.03138884785702 28.66356430319563 28.417337670549404 21.887709178397944 96-97 21.303230578430714 28.625513694808692 28.372797634798726 21.698458091961864 98-99 20.944826494165106 28.863829827934158 28.183579407360646 22.007764270540086 100-101 20.62330598826672 29.11370209410536 27.990055244508895 22.27293667311903 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 284.0 1 202.0 2 128.5 3 159.5 4 157.5 5 166.0 6 257.5 7 537.0 8 951.0 9 1195.5 10 1164.0 11 1113.5 12 1218.0 13 1332.5 14 1454.5 15 1635.0 16 1763.0 17 1826.5 18 1933.0 19 2061.0 20 2202.5 21 2528.5 22 2991.0 23 3591.0 24 4363.5 25 5381.0 26 6844.5 27 8634.5 28 10242.5 29 12064.0 30 14430.5 31 17201.5 32 20173.5 33 22938.5 34 25922.5 35 28599.0 36 32210.0 37 35207.0 38 36418.0 39 36744.0 40 35843.0 41 35092.0 42 34313.5 43 34512.5 44 35178.5 45 34932.0 46 33691.0 47 33064.5 48 35035.5 49 33841.5 50 30117.0 51 27622.5 52 25184.0 53 23637.0 54 21948.5 55 19689.0 56 17428.0 57 15214.0 58 13672.5 59 12147.5 60 9959.5 61 7960.5 62 6168.0 63 4565.0 64 3336.5 65 2530.5 66 1853.5 67 1384.0 68 964.5 69 604.5 70 415.0 71 277.0 72 207.5 73 163.5 74 121.0 75 96.0 76 63.0 77 29.0 78 18.5 79 10.5 80 9.0 81 8.0 82 5.0 83 2.0 84 0.5 85 0.0 86 0.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029224105317299395 2 0.005207204220173347 3 2.125389477621774E-4 4 4.250778955243548E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.012805471602671188 12-13 0.0 14-15 0.0 16-17 0.0 18-19 4.250778955243548E-4 20-21 0.0012220989496325202 22-23 0.0021785242145623187 24-25 0.011370833705276492 26-27 0.005047800009351714 28-29 1.062694738810887E-4 30-31 0.002816141057848851 32-33 0.004303913692184093 34-35 0.0 36-37 0.00913917475377363 38-39 0.012220989496325201 40-41 8.501557910487096E-4 42-43 0.0 44-45 0.0 46-47 2.125389477621774E-4 48-49 0.002763006320908306 50-51 0.0071200547500329435 52-53 0.0 54-55 0.004038240007481372 56-57 0.003613162111957016 58-59 4.250778955243548E-4 60-61 0.0026036021100866733 62-63 0.004569587376886815 64-65 0.0 66-67 0.003613162111957016 68-69 9.03290527989254E-4 70-71 7.43886317167621E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0031349494794921167 80-81 0.003188084216432661 82-83 0.01562161266052004 84-85 0.009670522123179074 86-87 0.007173189486973488 88-89 2.125389477621774E-4 90-91 0.001009560001870343 92-93 3.1880842164326616E-4 94-95 1.062694738810887E-4 96-97 0.002869275794789395 98-99 0.002869275794789395 100-101 5.844821063459879E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 941004.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.844100755410125 #Duplication Level Percentage of deduplicated Percentage of total 1 81.42235293744925 47.91225139993827 2 11.203862410572322 13.185624170749396 3 3.1695856255989203 5.595342477169274 4 1.2906906742086093 3.037981283087985 5 0.6797160225152303 1.9998639056976415 6 0.4213341395448017 1.4875817135441 7 0.2762809534425982 1.1380252982823913 8 0.2059698476684333 0.9696088375022212 9 0.1478425083430097 0.782969351118176 >10 1.0167712880142712 11.617279418822584 >50 0.10470452682675305 4.300151128085702 >100 0.05907148181676794 6.08066133367788 >500 9.087919995309468E-4 0.29134295742832544 >1k 7.270335996247575E-4 1.02889840325275 >5k 1.8175839990618937E-4 0.5724183216433179 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5352 0.5687542242115867 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4206 0.4469694071438591 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2803 0.29787333528869164 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1485 0.15781016871341674 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1126 0.11965942759010588 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.5940421082163308E-4 0.0 12-13 0.0 0.0 0.0 2.1253894776217743E-4 0.0 14-15 0.0 0.0 0.0 3.188084216432661E-4 0.0 16-17 0.0 0.0 0.0 4.2507789552435486E-4 0.0 18-19 0.0 0.0 0.0 5.313473694054436E-4 0.0 20-21 0.0 0.0 0.0 6.907515802270765E-4 0.0 22-23 0.0 0.0 0.0 0.0011689642126919758 0.0 24-25 0.0 0.0 0.0 0.0022316589515028627 0.0 26-27 0.0 0.0 0.0 0.002869275794789395 0.0 28-29 0.0 0.0 0.0 0.004888395798530081 0.0 30-31 0.0 0.0 0.0 0.008714096858249275 0.0 32-33 0.0 0.0 0.0 0.013071145287373912 0.0 34-35 0.0 0.0 0.0 0.015834151608282217 0.0 36-37 0.0 0.0 0.0 0.020563143195990667 0.0 38-39 0.0 0.0 0.0 0.028267680052369598 0.0 40-41 0.0 0.0 0.0 0.04473944850393835 0.0 42-43 0.0 0.0 0.0 0.06264585485290179 0.0 44-45 0.0 0.0 0.0 0.08379348015523844 0.0 46-47 0.0 0.0 0.0 0.12348512864982508 0.0 48-49 0.0 0.0 0.0 0.144951562373805 0.0 50-51 0.0 0.0 0.0 0.17120012242243393 0.0 52-53 0.0 0.0 0.0 0.2000522845811495 0.0 54-55 0.0 0.0 0.0 0.24160364886865518 0.0 56-57 0.0 0.0 0.0 0.3596158996136042 0.0 58-59 0.0 0.0 0.0 0.4687015145525417 0.0 60-61 0.0 0.0 0.0 0.5423993946890768 0.0 62-63 0.0 0.0 0.0 0.6384138643406404 0.0 64-65 0.0 0.0 0.0 0.7327280224101067 0.0 66-67 0.0 0.0 0.0 0.8873501069070907 0.0 68-69 0.0 0.0 0.0 1.0706649493519687 0.0 70-71 0.0 0.0 0.0 1.1683797305856298 0.0 72-73 0.0 0.0 0.0 1.2782623665786756 0.0 74-75 0.0 0.0 0.0 1.4773050911579548 0.0 76-77 0.0 0.0 0.0 1.723318923192675 0.0 78-79 0.0 0.0 0.0 1.9441468899175773 0.0 80-81 0.0 0.0 0.0 2.0995128607317293 0.0 82-83 0.0 0.0 0.0 2.3531249601489472 0.0 84-85 0.0 0.0 0.0 2.746268878772035 0.0 86-87 0.0 0.0 0.0 3.121400121572278 0.0 88-89 0.0 0.0 0.0 3.5480720592048494 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6510 0.0 29.998144 1 CGCGGAA 70 5.8038495E-7 27.145834 82-83 GGTATCA 4450 0.0 27.1211 1 ATACGTC 130 1.0022422E-5 25.575651 5 ATCAACG 7955 0.0 24.24012 3 GTACATG 4010 0.0 23.935392 1 TCAACGC 8115 0.0 23.762186 4 ATTCGCG 90 1.9503386E-7 23.752605 80-81 CAACGCA 8120 0.0 23.748817 5 TATCAAC 8200 0.0 23.515873 2 AACGCAG 8285 0.0 23.275846 6 AGTACTT 5005 0.0 23.059565 12-13 ACATGGG 4005 0.0 22.650602 3 CCCGCGA 95 3.2807293E-7 22.506054 20-21 GTGTAGC 410 0.0 22.01929 1 GAGTACT 4875 0.0 22.018246 12-13 TACATGG 4310 0.0 21.929296 2 GTGTACG 110 0.0028716077 21.597868 1 AGAGTAC 7480 0.0 21.208838 10-11 GTACTTT 5130 0.0 20.83119 14-15 >>END_MODULE