FastQCFastQC Report
Thu 26 May 2016
SRR936916_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936916_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979085
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45270.4623704785590628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33080.33786647737428316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21800.22265686840264123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50600.034.8470231
GGTATCA32000.031.0412031
ATCAACG62150.027.7620933
GTACATG39600.027.6041831
CAACGCA62550.027.5085645
TCAACGC62800.027.3990554
TATCAAC63700.027.0865632
AACGCAG64700.026.6665486
TACCGAG908.9114084E-426.4067022
TACATGG40400.026.1191062
ACATGGG40000.024.8355053
CGCAGAG70000.024.1697438
ACGCAGA70700.024.0648787
GAGTACT38100.022.26725212-13
GCAGAGT74850.021.96879
AGAGTAC59600.021.5695510-11
AGTACTT40950.021.18177212-13
CATGGGG14750.020.6240834
GTACTTT41200.020.53464914-15
CATGGGA23700.020.4568394
Produced by FastQC (version 0.11.5)