##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936916_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 979085 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.951216697222407 33.0 31.0 34.0 30.0 34.0 2 32.063263148756235 33.0 31.0 34.0 30.0 34.0 3 32.08377311469382 34.0 31.0 34.0 30.0 34.0 4 35.67010218724626 37.0 35.0 37.0 33.0 37.0 5 35.664885071265516 37.0 35.0 37.0 35.0 37.0 6 35.732132552332025 37.0 35.0 37.0 35.0 37.0 7 35.693961198465914 37.0 35.0 37.0 33.0 37.0 8 35.654766440094576 37.0 35.0 37.0 33.0 37.0 9 37.32181271289009 39.0 37.0 39.0 34.0 39.0 10-11 37.199549579454285 39.0 37.0 39.0 34.0 39.0 12-13 37.144640659391165 39.0 37.0 39.0 34.0 39.0 14-15 38.344326079962414 40.0 38.0 41.0 34.0 41.0 16-17 38.28529647579117 40.0 38.0 41.0 33.5 41.0 18-19 38.25487521512433 40.0 38.0 41.0 33.5 41.0 20-21 38.2061051900499 40.0 38.0 41.0 34.0 41.0 22-23 38.09346481664002 40.0 38.0 41.0 33.0 41.0 24-25 38.06435498450084 40.0 38.0 41.0 33.0 41.0 26-27 38.08275226359305 40.0 38.0 41.0 33.0 41.0 28-29 38.002926201504465 40.0 38.0 41.0 33.0 41.0 30-31 37.84700204783037 40.0 37.5 41.0 33.0 41.0 32-33 37.65128819254712 40.0 37.0 41.0 33.0 41.0 34-35 37.48841877875772 40.0 37.0 41.0 32.5 41.0 36-37 37.31630961561049 40.0 37.0 41.0 32.0 41.0 38-39 37.16513530490202 40.0 36.5 41.0 31.0 41.0 40-41 36.98588937630543 39.5 36.0 41.0 31.0 41.0 42-43 36.766846596567206 39.0 36.0 41.0 30.5 41.0 44-45 36.51086984276135 39.0 36.0 41.0 30.0 41.0 46-47 36.16629812529045 39.0 35.5 41.0 29.5 41.0 48-49 35.92803484886399 39.0 35.5 40.5 29.0 41.0 50-51 35.28140610876481 38.5 35.0 40.0 27.5 40.5 52-53 35.47777210354566 38.5 35.0 40.0 28.0 40.5 54-55 35.8816241695052 39.0 36.0 41.0 28.0 41.0 56-57 35.77720831184218 39.0 35.0 41.0 28.0 41.0 58-59 35.59593906555611 39.0 35.0 41.0 28.0 41.0 60-61 35.34392672750578 39.0 35.0 41.0 27.0 41.0 62-63 35.05385334266177 38.5 35.0 40.0 26.0 41.0 64-65 34.75497275517448 38.0 34.5 40.0 26.0 41.0 66-67 34.406040844257646 37.5 34.0 40.0 26.0 41.0 68-69 34.02770954513653 37.0 34.0 40.0 25.5 41.0 70-71 33.613984485514536 36.5 34.0 39.0 24.5 41.0 72-73 33.220042182241585 36.0 33.0 39.0 23.5 40.5 74-75 32.81423778323639 35.5 33.0 38.5 22.5 40.0 76-77 32.35112017853404 35.0 32.5 37.5 21.5 39.5 78-79 31.87608481388235 35.0 32.0 37.0 20.0 39.0 80-81 31.420590142837447 35.0 31.5 36.5 18.0 39.0 82-83 31.004740650709593 35.0 31.0 36.0 17.0 37.5 84-85 30.615178968118194 35.0 31.0 35.5 12.0 37.0 86-87 30.2561299580731 34.0 31.0 35.0 7.5 37.0 88-89 29.877628091534444 34.0 30.5 35.0 4.5 36.0 90-91 29.50611080753969 34.0 30.0 35.0 2.0 36.0 92-93 29.136561687698208 34.0 29.5 35.0 2.0 36.0 94-95 28.765347237471723 34.0 29.0 35.0 2.0 35.0 96-97 28.304123237512577 34.0 29.0 35.0 2.0 35.0 98-99 27.771108228601193 34.0 29.0 35.0 2.0 35.0 100-101 25.636819581548078 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24390301074361176 1101 2 0.23741537564470505 1101 3 0.09850582449298884 1101 4 0.13894408529956337 1101 5 0.14158332457388667 1101 6 0.09413076204521786 1101 7 0.1403802695099472 1101 8 0.11820502588413007 1101 9 0.09303733996817698 1101 10-11 0.1802830852604842 1101 12-13 0.175833569962613 1101 14-15 0.21651627057060097 1101 16-17 0.28241024903444156 1101 18-19 0.2731660022718856 1101 20-21 0.2600398209499559 1101 22-23 0.26506617136655564 1101 24-25 0.3906968261427224 1101 26-27 0.3130508350757424 1101 28-29 0.12408033961784071 1101 30-31 0.16507919483918698 1101 32-33 0.06641688939826196 1101 34-35 0.15903743728542707 1101 36-37 0.1139442887571036 1101 38-39 0.06794982320617038 1101 40-41 0.24133977306494359 1101 42-43 0.2940703834872238 1101 44-45 0.41046380552814554 1101 46-47 0.31631817416698027 1101 48-49 0.41784439249307326 1101 50-51 0.5027381115225324 1101 52-53 0.5154554455542808 1101 54-55 0.4855661833871636 1101 56-57 0.48100761632077393 1101 58-59 0.5441242297236926 1101 60-61 0.5906328495062709 1101 62-63 0.5977956804439728 1101 64-65 0.3830226984161058 1101 66-67 0.38684846976929776 1101 68-69 0.3531381030237313 1101 70-71 0.40112431397231063 1101 72-73 0.5279231305944876 1101 74-75 0.49255773404274095 1101 76-77 0.5920311358132295 1101 78-79 0.7768526136854383 1101 80-81 0.6554420284925335 1101 82-83 0.7611567501768306 1101 84-85 0.5264570027344035 1101 86-87 0.7079585911511934 1101 88-89 0.673876099220923 1101 90-91 0.8800368412333661 1101 92-93 0.8262929703419779 1101 94-95 0.8422453056017538 1101 96-97 0.7228668071228519 1101 98-99 0.6918438770901112 1101 100-101 0.7613894958120824 1102 1 0.09064692803755037 1102 2 0.030398270148662476 1102 3 -0.07381512999993944 1102 4 0.08119559751703065 1102 5 0.02891401347874023 1102 6 0.03181178566808285 1102 7 -0.16033913997043214 1102 8 -0.18843831983845405 1102 9 -0.008537864870916678 1102 10-11 0.018024493470704783 1102 12-13 -0.03967069339747553 1102 14-15 -0.25537053956151823 1102 16-17 -0.062005738305614955 1102 18-19 -0.23901502655655094 1102 20-21 -0.19450932481635164 1102 22-23 -0.25493778486502094 1102 24-25 -0.2263722309207168 1102 26-27 -0.10545287649415513 1102 28-29 -0.26724093762029355 1102 30-31 -0.24549123128860373 1102 32-33 -0.3411582529740187 1102 34-35 -0.3383295864831908 1102 36-37 -0.2153776339714497 1102 38-39 -0.11916327296148665 1102 40-41 -0.2626581030633659 1102 42-43 -0.047035903797649325 1102 44-45 -0.02484972582498557 1102 46-47 0.11534993854215969 1102 48-49 0.2945765452036113 1102 50-51 0.16351832203569927 1102 52-53 0.310327961197018 1102 54-55 0.08320389668531902 1102 56-57 0.1472245219230075 1102 58-59 0.024894861991974437 1102 60-61 0.043533912483233905 1102 62-63 -0.10925682291362904 1102 64-65 0.14135525091425905 1102 66-67 0.06559205390689016 1102 68-69 0.13773378656684798 1102 70-71 0.026318221375674966 1102 72-73 0.018143950783766627 1102 74-75 0.27623411429906497 1102 76-77 0.01851706516326601 1102 78-79 0.011209361663077289 1102 80-81 0.10021511778884218 1102 82-83 0.1655637839401436 1102 84-85 0.07430970598580444 1102 86-87 0.06777056603953469 1102 88-89 0.10689189291385759 1102 90-91 0.07874065483239079 1102 92-93 0.1631931587835318 1102 94-95 0.30250600044095677 1102 96-97 0.022083889714391347 1102 98-99 0.1557692917135931 1102 100-101 0.11748781857811252 1103 1 0.15886037867483438 1103 2 0.176931998503985 1103 3 0.13400019953581932 1103 4 0.1442173306644179 1103 5 0.11798083186345565 1103 6 0.2078028676073913 1103 7 0.25727680522605567 1103 8 0.07674724566974334 1103 9 0.011346912674810028 1103 10-11 0.12680586915557512 1103 12-13 -0.03438022041184752 1103 14-15 0.255183346941358 1103 16-17 0.35569231040604876 1103 18-19 0.2561295334495597 1103 20-21 0.3012909069237111 1103 22-23 0.365052686501663 1103 24-25 0.2816186382432804 1103 26-27 0.33717503731335086 1103 28-29 0.3301712983475156 1103 30-31 0.2816149551534508 1103 32-33 0.16223967616718227 1103 34-35 0.3141706390123815 1103 36-37 0.3812704611113631 1103 38-39 0.40658970878377687 1103 40-41 0.41736368327784845 1103 42-43 0.474502739204695 1103 44-45 0.6174715140074767 1103 46-47 0.6168611057766071 1103 48-49 0.5905180875007972 1103 50-51 0.5197263885326109 1103 52-53 0.5391578589029677 1103 54-55 0.5170562167456296 1103 56-57 0.5605685402771883 1103 58-59 0.7629055516383687 1103 60-61 0.6892405924714851 1103 62-63 0.8041410830998288 1103 64-65 0.7256702416284639 1103 66-67 0.5091214874388967 1103 68-69 0.5248937219016625 1103 70-71 0.5587643741899697 1103 72-73 0.4742056186183774 1103 74-75 0.6373131163828702 1103 76-77 0.5457395841916224 1103 78-79 0.8044326994138125 1103 80-81 0.651200706887348 1103 82-83 0.71995054006495 1103 84-85 0.688139869660894 1103 86-87 0.8698485944450383 1103 88-89 0.758298869631151 1103 90-91 0.8443348876964585 1103 92-93 0.9296946933097949 1103 94-95 0.9862497357114677 1103 96-97 1.0068651358838565 1103 98-99 0.9135960694859726 1103 100-101 0.9253524102229846 1104 1 0.06186361186386691 1104 2 -0.01576299961396188 1104 3 0.05652803723312161 1104 4 0.02600293204250903 1104 5 0.11032714306378466 1104 6 0.0062668699568106945 1104 7 0.09413307816615912 1104 8 0.09746573577429984 1104 9 0.1387063982575043 1104 10-11 0.2620940919864623 1104 12-13 0.01754448863675151 1104 14-15 -0.09746793929367215 1104 16-17 0.1351209876586168 1104 18-19 0.2177767120387344 1104 20-21 0.19751956532832793 1104 22-23 0.16340601198534443 1104 24-25 0.05183784966514082 1104 26-27 0.13858266075777692 1104 28-29 0.04590954865584962 1104 30-31 0.17682560555536497 1104 32-33 -0.08349666195174166 1104 34-35 -0.09487051031755556 1104 36-37 -0.03153041422211089 1104 38-39 0.17057731954967892 1104 40-41 0.2613512173370154 1104 42-43 0.09195417972985354 1104 44-45 0.051433312955829535 1104 46-47 -9.259267300620877E-4 1104 48-49 0.21488738004002528 1104 50-51 -5.222727978306807E-4 1104 52-53 0.17621663763306827 1104 54-55 0.28079535246888554 1104 56-57 0.24428802811725348 1104 58-59 0.19887076622820388 1104 60-61 0.36599757401749144 1104 62-63 0.18888838644703299 1104 64-65 0.12702345807899462 1104 66-67 0.09024105516355263 1104 68-69 0.09441714841359072 1104 70-71 0.19584835656277022 1104 72-73 0.0827256552403739 1104 74-75 0.20205771307801257 1104 76-77 0.16882787419569212 1104 78-79 0.28064402209549755 1104 80-81 0.3765897607261621 1104 82-83 0.17622495951821548 1104 84-85 0.1995390389269147 1104 86-87 0.2500811583184621 1104 88-89 0.06939482811746345 1104 90-91 0.12059420688032318 1104 92-93 0.06569382323622008 1104 94-95 0.18929384263408622 1104 96-97 -0.05129774868400716 1104 98-99 -0.18017463106023612 1104 100-101 0.013892807005024821 1105 1 0.022728824009952575 1105 2 -0.00114750886362458 1105 3 -0.013217486525185507 1105 4 -0.013023598158191874 1105 5 -0.04165032802328028 1105 6 -0.044955981606385365 1105 7 0.12689410824126668 1105 8 0.08276816732610115 1105 9 -0.010718173399510533 1105 10-11 0.04228117253228447 1105 12-13 -0.060749498110531874 1105 14-15 0.13208073950304566 1105 16-17 0.22262607329183481 1105 18-19 0.14112923221322404 1105 20-21 0.16937222170933808 1105 22-23 0.004408421248797367 1105 24-25 0.030869828126817822 1105 26-27 0.11375026553950818 1105 28-29 -0.04662208840505855 1105 30-31 0.07397623582010482 1105 32-33 -0.08747636894850785 1105 34-35 -0.20437949464142946 1105 36-37 -0.16023673526618865 1105 38-39 0.025368782053966754 1105 40-41 0.2624482989795567 1105 42-43 0.33986880477274894 1105 44-45 0.11091629421593296 1105 46-47 0.1862069789973333 1105 48-49 0.10866044228109928 1105 50-51 -0.003669527971020159 1105 52-53 0.2793377128509178 1105 54-55 0.2897955965792818 1105 56-57 0.2469796105540425 1105 58-59 0.12113009637513272 1105 60-61 0.19586430389341558 1105 62-63 0.16368345753976143 1105 64-65 0.19006125273615737 1105 66-67 0.16082822910812666 1105 68-69 0.16177392813757052 1105 70-71 0.22305074530680002 1105 72-73 0.37922992363444763 1105 74-75 0.5312062630976513 1105 76-77 0.5886124909954376 1105 78-79 0.45557565349588103 1105 80-81 0.37152464693179965 1105 82-83 0.27311272887988736 1105 84-85 0.3630243693073396 1105 86-87 0.6718922451757727 1105 88-89 0.761711718787847 1105 90-91 0.4011410849206456 1105 92-93 0.5972537594137357 1105 94-95 0.7109793364623371 1105 96-97 0.49617853899832554 1105 98-99 0.3811982599497803 1105 100-101 0.5554386272336416 1106 1 0.13330289781459115 1106 2 0.1646951725077095 1106 3 0.2170299030564209 1106 4 0.17763951843645032 1106 5 0.10675723188418118 1106 6 0.16855369136990817 1106 7 0.1294383265221981 1106 8 0.19191251267245235 1106 9 0.05723951672575822 1106 10-11 0.15587959730906675 1106 12-13 0.2691913825207237 1106 14-15 0.17374558748527136 1106 16-17 0.2045473800287212 1106 18-19 0.3066038118546288 1106 20-21 0.12054272701261937 1106 22-23 -0.009325313650059286 1106 24-25 -4.45007973340239E-4 1106 26-27 -0.10742989091585287 1106 28-29 -0.04880790460638451 1106 30-31 0.07539529466303208 1106 32-33 -0.07571408797873858 1106 34-35 0.27961317242291983 1106 36-37 -0.009399897934180501 1106 38-39 -0.14974385513546196 1106 40-41 -0.03968950395265125 1106 42-43 0.004498059888106809 1106 44-45 0.14261814636416403 1106 46-47 0.11214723885096589 1106 48-49 0.2891272864513681 1106 50-51 0.21793450320365793 1106 52-53 0.3454228854804384 1106 54-55 0.5142843037613929 1106 56-57 0.3950442878224223 1106 58-59 0.5561991821345984 1106 60-61 0.6504646055525356 1106 62-63 0.6091825772964938 1106 64-65 0.46074017976084036 1106 66-67 0.5187783050078849 1106 68-69 0.6018737375394423 1106 70-71 0.600711480637294 1106 72-73 0.6266112605372527 1106 74-75 0.8469269713661163 1106 76-77 0.7271753908028984 1106 78-79 0.6248224271496916 1106 80-81 0.8782557192766411 1106 82-83 0.9587402720069562 1106 84-85 0.9696807543446582 1106 86-87 1.0229497495527404 1106 88-89 1.2028508457223204 1106 90-91 1.096093386890157 1106 92-93 1.0069432251084969 1106 94-95 1.1109531474064553 1106 96-97 1.0598848723850018 1106 98-99 0.7445195172184569 1106 100-101 0.7902181784308198 1107 1 0.5062865020705196 1107 2 0.4586630613633069 1107 3 0.4679660506370169 1107 4 0.3396679686836208 1107 5 0.3284318493933185 1107 6 0.41255681231180574 1107 7 0.3945793493596099 1107 8 0.5123098658113463 1107 9 0.5042253509972667 1107 10-11 0.5338901858333998 1107 12-13 0.6996053122887318 1107 14-15 0.7087502176023648 1107 16-17 0.66442599806701 1107 18-19 0.6426456961907903 1107 20-21 0.74832180968248 1107 22-23 0.7948423316856719 1107 24-25 0.7081228684632777 1107 26-27 0.8859421572157657 1107 28-29 0.726064463448779 1107 30-31 0.8853431619744043 1107 32-33 0.8155571288916192 1107 34-35 0.7187669544051545 1107 36-37 0.6274497595991377 1107 38-39 0.6804401886441198 1107 40-41 0.7433024426133699 1107 42-43 0.8341596968707279 1107 44-45 0.695476399986589 1107 46-47 0.552746751271286 1107 48-49 0.845698408019615 1107 50-51 0.7581874992808793 1107 52-53 0.7207073744531982 1107 54-55 0.7253757964949443 1107 56-57 0.752166703798629 1107 58-59 0.9282587878052055 1107 60-61 1.1659977709597626 1107 62-63 1.0849048350657782 1107 64-65 0.9866425126379141 1107 66-67 1.0966101683127931 1107 68-69 1.1757902496438888 1107 70-71 1.0083750668587115 1107 72-73 1.0453479810104653 1107 74-75 1.0939403433602521 1107 76-77 0.9894188782664912 1107 78-79 1.0618278618002428 1107 80-81 1.1121711244726349 1107 82-83 1.091882461147911 1107 84-85 1.0832131152184132 1107 86-87 1.3028592260390361 1107 88-89 1.0551278374151103 1107 90-91 1.0084498600098328 1107 92-93 1.2898481038750198 1107 94-95 1.5058258664325912 1107 96-97 1.3194623303292339 1107 98-99 1.380787372866795 1107 100-101 1.6381215318234936 1108 1 0.10466769064302639 1108 2 0.16890442506404568 1108 3 0.22547204237771723 1108 4 0.013924173571986387 1108 5 0.05702613009282942 1108 6 -0.0296711432201775 1108 7 0.014691880220894404 1108 8 -0.15136255051951508 1108 9 -0.08606178043778101 1108 10-11 -0.09573136943642169 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2316 88-89 -3.6744352082428584 2316 90-91 -3.7720764171660726 2316 92-93 -4.1872770581410705 2316 94-95 -4.58185387972182 2316 96-97 -5.035466015761596 2316 98-99 -5.200214559098672 2316 100-101 -5.8161226783939135 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1790.0 3 543.0 4 546.0 5 605.0 6 648.0 7 762.0 8 918.0 9 1134.0 10 1552.0 11 1929.0 12 2749.0 13 3706.0 14 4202.0 15 5363.0 16 7006.0 17 9615.0 18 8455.0 19 4901.0 20 4607.0 21 4760.0 22 5084.0 23 5638.0 24 6251.0 25 7214.0 26 8613.0 27 10150.0 28 12225.0 29 14965.0 30 18287.0 31 22748.0 32 30066.0 33 39625.0 34 56059.0 35 82765.0 36 129569.0 37 206607.0 38 214561.0 39 42846.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.4602793473041 20.06447262212504 15.201438628398606 22.273809402172247 2 14.355071057121549 20.768091214663002 42.099326719725376 22.777511008490077 3 18.260351656479575 26.25562776024592 30.94111168888485 24.542908894389658 4 11.743852976251912 17.551348252488598 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20.786056621735916 27.26238728156014 31.251710950491734 20.699845146212212 26-27 20.43907488674068 27.43497962583441 31.235416474092204 20.890529013332706 28-29 20.254808559058926 27.301369394270438 31.504086528271685 20.93973551839895 30-31 20.55788864596149 27.26304236691298 31.35413043822435 20.824938548901184 32-33 20.30647681889217 27.42280624699958 31.254404870126557 21.016312063981697 34-35 20.79283769911595 27.44171437618423 30.968575558086442 20.796872366613382 36-37 20.821786840419893 27.027322415534826 31.03544079598534 21.115449948059933 38-39 20.434864765484555 27.60367298578199 31.04852712861579 20.912935120117666 40-41 20.69558129474872 27.38929232994402 31.188748616585045 20.72637775872222 42-43 20.46455166045913 27.438750668376482 30.80624461532412 21.29045305584027 44-45 20.67920889868962 27.574508549223726 30.529060619539756 21.217221932546902 46-47 20.845596214784912 27.710971352989695 30.153333895089485 21.29009853713591 48-49 20.75709695408556 27.868554421386566 30.021234176504112 21.35311444802376 50-51 20.748971210228557 27.993908461457533 29.85078590448214 21.406334423831765 52-53 20.941936169213076 28.18781115108281 29.61126670793217 21.258985971771946 54-55 20.83496132404804 27.97079576186135 29.749200050052732 21.445042864037877 56-57 20.777145390324474 28.31872327646613 29.40773600978952 21.496395323419875 58-59 20.89925662024714 27.95625723921956 29.610833855953068 21.53365228458023 60-61 21.01750398978615 27.970430896903924 29.83042451324609 21.181640600063837 62-63 20.78161588768102 27.949254211578683 30.049361719514096 21.219768181226204 64-65 20.72411981028829 27.964551875795724 29.860443310936102 21.450885002979884 66-67 20.69740500936779 28.206604241964843 29.34223188399875 21.75375886466861 68-69 20.697132025923402 28.04064772735981 29.67432260174066 21.587897644976128 70-71 20.743574925436587 27.998542711282592 29.641058343156747 21.616824020124074 72-73 20.81528866699624 27.863637477886773 29.56758170148266 21.753492153634333 74-75 20.645162278860386 28.239065214926406 29.445494875700103 21.670277630513105 76-77 20.724199209182515 28.06942357976443 29.50210985119943 21.704267359853628 78-79 20.83105382500221 27.94066790998841 29.36849573904342 21.85978252596596 80-81 20.663691623030765 28.148403298986775 29.23496541665091 21.952939661331552 82-83 20.87914613987303 28.09010402512459 29.26322177519049 21.76752805981189 84-85 20.769156515250494 28.004884733267723 29.22989861267242 21.99606013880937 86-87 20.59212526021379 28.013537502713888 29.490964355499933 21.903372881572395 88-89 20.796815110241862 27.864873122485623 29.242832516367418 22.0954792509051 90-91 20.50007561266696 27.838080582667125 29.626064708094233 22.03577909657168 92-93 20.753101393417413 27.907125260429172 29.537291822702784 21.80248152345063 94-95 20.55482490302184 27.68240705035714 29.441472178312132 22.32129586830889 96-97 20.56244419791073 27.6915250289609 29.46059172177921 22.285439051349158 98-99 20.280075408847768 27.80164816356976 29.39545196773155 22.522824459850916 100-101 20.23506154061599 27.73053581887709 29.56295480820242 22.471447832304506 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 143.0 1 110.5 2 109.5 3 152.0 4 148.0 5 157.5 6 242.5 7 486.0 8 855.5 9 1072.0 10 1034.5 11 968.0 12 1069.0 13 1179.5 14 1204.5 15 1310.0 16 1408.5 17 1462.0 18 1616.5 19 1808.0 20 1979.5 21 2265.0 22 2829.0 23 3557.0 24 4324.5 25 5348.0 26 7062.0 27 9056.0 28 10525.0 29 12206.0 30 14548.0 31 17292.0 32 20356.5 33 23738.0 34 27421.0 35 30367.0 36 34223.5 37 37912.5 38 38503.5 39 37999.0 40 37082.5 41 36613.0 42 36283.5 43 36363.0 44 37031.0 45 36966.0 46 35540.0 47 34545.5 48 36481.0 49 35637.5 50 31692.5 51 28870.0 52 26551.5 53 24893.5 54 23071.0 55 20665.0 56 18406.5 57 16109.0 58 14215.0 59 12450.5 60 10376.5 61 8269.5 62 6327.5 63 4687.0 64 3370.0 65 2498.5 66 1780.5 67 1317.0 68 937.5 69 594.5 70 417.5 71 291.5 72 212.0 73 168.0 74 125.0 75 90.0 76 58.5 77 31.5 78 22.5 79 13.5 80 10.0 81 7.0 82 7.0 83 6.5 84 3.5 85 2.5 86 2.0 87 1.0 88 1.5 89 1.5 90 0.0 91 0.5 92 1.5 93 3.5 94 2.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03830106681238095 2 0.03013017255907301 3 0.023695593334593014 4 0.05331508500283428 5 0.09682509690169903 6 0.11949932845462855 7 0.06342656664130285 8 0.09202469652788062 9 0.075172227130433 10-11 0.0792066061680038 12-13 0.07583611228851428 14-15 0.06797162656970539 16-17 0.055000331942579035 18-19 0.0669502647880419 20-21 0.060209277029062855 22-23 0.03482843675472507 24-25 0.009907209282135872 26-27 0.0027066087214082536 28-29 0.007711281451559365 30-31 0.005566421710066031 32-33 0.005617489799149205 34-35 0.007149532471644444 36-37 0.007353804827977142 38-39 0.004596128017485714 40-41 0.007813417629725712 42-43 0.003421561968572698 44-45 0.0019916554742438093 46-47 0.001276702227079365 48-49 3.0640853449904755E-4 50-51 0.002246995919659682 52-53 0.006026034511814602 54-55 0.01200100093454603 56-57 0.008068758075141586 58-59 0.004391855661153015 60-61 0.002298064008742857 62-63 0.008936915589555554 64-65 0.0020937916524101582 66-67 0.02032509945510349 68-69 0.04417389705694603 70-71 0.05673664697140698 72-73 0.003370493879489523 74-75 0.012460613736294602 76-77 0.022929571998345395 78-79 0.026095793521502222 80-81 0.0739465929924368 82-83 0.028904538421076823 84-85 0.011898864756379681 86-87 0.03293891745864762 88-89 0.04739118666918603 90-91 0.042182241582702215 92-93 0.04208010540453587 94-95 0.013022362716209521 96-97 0.018282375891776507 98-99 0.04289719482986666 100-101 0.08048330839508316 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 979085.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.465213255745866 #Duplication Level Percentage of deduplicated Percentage of total 1 82.88540496980235 50.945690922577604 2 10.44765939165988 12.843352250635414 3 2.8701917833556743 5.292508501465383 4 1.142527280115085 2.80902731691124 5 0.5959674634362464 1.8315633616797407 6 0.3712040023595929 1.3689679899851261 7 0.26051140482010754 1.1208672336975298 8 0.18319624652923117 0.900815708845711 9 0.1447277316933195 0.8006148803295229 >10 0.9485065317613882 11.519539902137604 >50 0.09874654898776869 4.172441034670103 >100 0.05002702372217709 4.998024517833166 >500 8.310135981920992E-4 0.37348546576488084 >1k 4.986081589152595E-4 1.0231009134669808 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4527 0.4623704785590628 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3308 0.33786647737428316 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2180 0.22265686840264123 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 5.10680890831746E-5 0.0 16-17 0.0 0.0 0.0 1.021361781663492E-4 0.0 18-19 0.0 0.0 0.0 1.021361781663492E-4 0.0 20-21 0.0 0.0 0.0 3.064085344990476E-4 0.0 22-23 0.0 0.0 0.0 6.128170689980951E-4 0.0 24-25 0.0 0.0 0.0 0.0012256341379961902 0.0 26-27 0.0 0.0 0.0 0.0019405873851606346 0.0 28-29 0.0 0.0 0.0 0.00377903859215492 0.0 30-31 0.0 0.0 0.0 0.007813417629725712 0.0 32-33 0.0 0.0 0.0 0.012001000934546029 0.0 34-35 0.0 0.0 0.0 0.01623965232844952 0.0 36-37 0.0 0.0 0.0 0.022265686840264122 0.0 38-39 0.0 0.0 0.0 0.030487649182655237 0.0 40-41 0.0 0.0 0.0 0.04580807590760762 0.0 42-43 0.0 0.0 0.0 0.06357977090855238 0.0 44-45 0.0 0.0 0.0 0.08395593845273903 0.0 46-47 0.0 0.0 0.0 0.12077603068170792 0.0 48-49 0.0 0.0 0.0 0.14380773885821968 0.0 50-51 0.0 0.0 0.0 0.17015887282513775 0.0 52-53 0.0 0.0 0.0 0.19916554742438092 0.0 54-55 0.0 0.0 0.0 0.24313517212499425 0.0 56-57 0.0 0.0 0.0 0.36227702395604056 0.0 58-59 0.0 0.0 0.0 0.4603277549957358 0.0 60-61 0.0 0.0 0.0 0.5324869648702615 0.0 62-63 0.0 0.0 0.0 0.6277289510103821 0.0 64-65 0.0 0.0 0.0 0.7205196688745104 0.0 66-67 0.0 0.0 0.0 0.8671361526323047 0.0 68-69 1.021361781663492E-4 0.0 0.0 1.0473043709177445 0.0 70-71 1.021361781663492E-4 0.0 0.0 1.1416781995434513 0.0 72-73 1.021361781663492E-4 0.0 0.0 1.2496361398652822 0.0 74-75 1.021361781663492E-4 0.0 0.0 1.4546234494451453 0.0 76-77 1.021361781663492E-4 0.0 0.0 1.6883620931788355 0.0 78-79 1.021361781663492E-4 0.0 0.0 1.902337386437337 0.0 80-81 1.021361781663492E-4 0.0 0.0 2.0446130826230613 0.0 82-83 1.021361781663492E-4 0.0 0.0 2.289331365509634 0.0 84-85 1.021361781663492E-4 0.0 0.0 2.668869403575788 0.0 86-87 1.021361781663492E-4 0.0 0.0 3.039521594141469 0.0 88-89 1.021361781663492E-4 0.0 0.0 3.473293942813954 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5060 0.0 34.847023 1 GGTATCA 3200 0.0 31.041203 1 ATCAACG 6215 0.0 27.762093 3 GTACATG 3960 0.0 27.604183 1 CAACGCA 6255 0.0 27.508564 5 TCAACGC 6280 0.0 27.399055 4 TATCAAC 6370 0.0 27.086563 2 AACGCAG 6470 0.0 26.666548 6 TACCGAG 90 8.9114084E-4 26.406702 2 TACATGG 4040 0.0 26.119106 2 ACATGGG 4000 0.0 24.835505 3 CGCAGAG 7000 0.0 24.169743 8 ACGCAGA 7070 0.0 24.064878 7 GAGTACT 3810 0.0 22.267252 12-13 GCAGAGT 7485 0.0 21.9687 9 AGAGTAC 5960 0.0 21.56955 10-11 AGTACTT 4095 0.0 21.181772 12-13 CATGGGG 1475 0.0 20.624083 4 GTACTTT 4120 0.0 20.534649 14-15 CATGGGA 2370 0.0 20.456839 4 >>END_MODULE