FastQCFastQC Report
Thu 26 May 2016
SRR936916_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936916_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979085
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55520.5670600611795708No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43380.44306674088562276No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30010.30651067067721394No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15030.15351067578402283No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10810.11040920859782348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68750.032.135221
GGTATCA45250.031.3945031
ATCAACG84050.025.997553
TCAACGC84300.025.920454
TATCAAC85500.025.8357642
CAACGCA84850.025.7524365
AACGCAG86750.025.2470286
GTACATG40300.024.6400871
GTATAGG4050.023.4625931
ACATGGG41100.022.7686183
GAGTACT54950.022.60907712-13
ACGCAGA96650.022.5134747
CGCAGAG96500.022.3526788
AGTACTT56500.022.32517812-13
TACATGG44550.022.1794152
CATGGGG15550.021.9945164
TCGCGGT652.9437087E-421.92178226-27
GTACTTT57500.021.27265414-15
GCAGAGT101450.021.0747039
AGAGTAC80800.020.87459410-11
Produced by FastQC (version 0.11.5)