FastQCFastQC Report
Thu 26 May 2016
SRR936915_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936915_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989277
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44180.4465887713956758No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32850.33206068674395545No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28370.2867750892823749No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20620.2084350490307568No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19920.20135917442738485No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14830.14990745766858019No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12140.12271588240705081No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACCG650.00582145529.2535725
GTACATG44900.026.4669531
TACATGG45550.025.5700742
GTATAGG3000.025.351811
ACATGGG45200.024.5072023
GAGTACT35950.022.34364712-13
GTATCAA85650.021.4225031
AGTACTT38200.020.90317212-13
CATGGGA28500.020.5180684
GTACTTT39700.020.11541714-15
ATGGGAG12250.019.4028785
AGAGTAC66550.019.32001510-11
TCAACGC97100.018.7523564
CAACGCA96700.018.7296985
CATGGGG19050.018.7172724
ATCAACG97850.018.6086223
TATCAAC98000.018.5791972
AACGCAG98150.018.5014326
GTATAGC4303.6379788E-1217.687311
GTACCTG7000.017.6557241
Produced by FastQC (version 0.11.5)