##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936915_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 989277 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.766614406278524 33.0 31.0 34.0 30.0 34.0 2 31.865366323082412 33.0 31.0 34.0 30.0 34.0 3 31.877125415833987 33.0 31.0 34.0 30.0 34.0 4 35.44860438481841 37.0 35.0 37.0 33.0 37.0 5 35.47129469299296 37.0 35.0 37.0 33.0 37.0 6 35.53803029889505 37.0 35.0 37.0 33.0 37.0 7 35.476369105922814 37.0 35.0 37.0 33.0 37.0 8 35.44906734918531 37.0 35.0 37.0 33.0 37.0 9 37.07304223185215 39.0 37.0 39.0 33.0 39.0 10-11 36.93964177879401 39.0 37.0 39.0 33.0 39.0 12-13 36.8393407508716 39.0 37.0 39.0 33.0 39.0 14-15 37.9799211949737 40.0 38.0 41.0 33.0 41.0 16-17 37.90862973666627 40.0 38.0 41.0 33.0 41.0 18-19 37.87539789159153 40.0 38.0 41.0 33.0 41.0 20-21 37.81941963676503 40.0 38.0 41.0 32.5 41.0 22-23 37.68135668776288 40.0 37.0 41.0 32.0 41.0 24-25 37.494344859933065 40.0 37.0 41.0 32.0 41.0 26-27 37.453218360479426 40.0 37.0 41.0 32.0 41.0 28-29 37.53870099072353 40.0 37.0 41.0 32.0 41.0 30-31 37.40245098187869 40.0 37.0 41.0 32.0 41.0 32-33 37.22020627185308 40.0 36.5 41.0 31.0 41.0 34-35 37.01133302401653 39.0 36.0 41.0 31.0 41.0 36-37 36.855361541812854 39.0 36.0 41.0 30.5 41.0 38-39 36.68468184340685 39.0 36.0 41.0 30.0 41.0 40-41 36.480330079441856 39.0 35.0 41.0 30.0 41.0 42-43 36.27201532027935 39.0 35.0 40.5 30.0 41.0 44-45 36.02593257500175 39.0 35.0 40.0 29.5 41.0 46-47 35.67179111613835 39.0 35.0 40.0 27.5 41.0 48-49 35.31402529321919 38.5 35.0 40.0 26.5 41.0 50-51 34.680732494538944 38.0 34.0 39.5 25.5 40.5 52-53 35.00762021152822 38.5 34.5 40.0 26.5 40.5 54-55 35.315882710302574 39.0 35.0 40.0 26.5 41.0 56-57 35.1931658170563 39.0 35.0 40.5 26.0 41.0 58-59 34.95269676743723 38.5 34.5 40.0 26.0 41.0 60-61 34.64360740217351 38.0 34.0 40.0 25.5 41.0 62-63 34.359063235069655 38.0 34.0 40.0 25.0 41.0 64-65 34.050439361270904 37.0 34.0 40.0 23.5 41.0 66-67 33.65525884054719 37.0 33.5 39.5 22.5 41.0 68-69 33.21397798594327 36.0 33.0 39.0 22.0 41.0 70-71 32.82774945743204 36.0 32.5 39.0 21.5 40.5 72-73 32.447979686174854 35.0 32.0 38.5 20.0 40.0 74-75 32.01583378568389 35.0 32.0 37.5 19.0 39.5 76-77 31.56893317038605 35.0 31.0 37.0 18.0 39.0 78-79 31.112626696061874 35.0 31.0 36.5 14.0 39.0 80-81 30.66069816643872 35.0 31.0 36.0 9.5 38.5 82-83 30.23493369400077 34.0 30.0 35.5 7.0 37.0 84-85 29.87038615069389 34.0 30.0 35.0 4.5 37.0 86-87 29.54276001564779 34.0 30.0 35.0 2.0 36.0 88-89 29.146670750457154 34.0 29.0 35.0 2.0 36.0 90-91 28.780167738661667 34.0 29.0 35.0 2.0 36.0 92-93 28.40522876808012 34.0 29.0 35.0 2.0 35.0 94-95 28.005330155254796 34.0 29.0 35.0 2.0 35.0 96-97 27.547126335697687 34.0 27.0 35.0 2.0 35.0 98-99 26.925045765746095 34.0 26.5 35.0 2.0 35.0 100-101 24.720572195654 31.0 21.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4357827613987908 1101 2 0.3661543870279367 1101 3 0.4022001761682894 1101 4 0.38549568685246527 1101 5 0.2883710337043226 1101 6 0.3173336291050859 1101 7 0.23942764076443268 1101 8 0.29921073222940464 1101 9 0.2688430584962447 1101 10-11 0.39241957058765564 1101 12-13 0.39877253935375734 1101 14-15 0.6861736547058186 1101 16-17 0.6716456808781004 1101 18-19 0.7777869352388649 1101 20-21 0.7267374486325267 1101 22-23 0.8040615019496329 1101 24-25 0.9492235372973994 1101 26-27 0.9289279282623326 1101 28-29 0.8637623538768722 1101 30-31 0.7168455366002249 1101 32-33 0.7807265225953373 1101 34-35 0.8822809220828063 1101 36-37 0.7127943796940741 1101 38-39 0.724383736249635 1101 40-41 0.6593082842727043 1101 42-43 0.7311215825928556 1101 44-45 0.7719016722480632 1101 46-47 0.7861591626947728 1101 48-49 0.9016272214559748 1101 50-51 0.608799308635561 1101 52-53 0.6900293859049569 1101 54-55 0.7357105684076473 1101 56-57 0.5799909403350796 1101 58-59 0.6914457465024739 1101 60-61 0.7027505412028816 1101 62-63 0.887871385874611 1101 64-65 0.7616657165982375 1101 66-67 0.6315113290299763 1101 68-69 0.7472917950235711 1101 70-71 0.7651793778116982 1101 72-73 0.805705163301333 1101 74-75 0.5668646637417858 1101 76-77 0.5701440706494019 1101 78-79 0.8120322341761934 1101 80-81 1.052105206774776 1101 82-83 0.8280214431537765 1101 84-85 0.841796265999303 1101 86-87 1.0524078113931061 1101 88-89 0.983224943060975 1101 90-91 1.0249204421324158 1101 92-93 0.9526648225369492 1101 94-95 1.2753968205909132 1101 96-97 1.2753768244859351 1101 98-99 1.407060084259811 1101 100-101 1.7937876416636875 1102 1 0.2694235207834623 1102 2 0.2913627076159777 1102 3 0.3619771903187363 1102 4 0.1687331948315034 1102 5 0.19735406909602204 1102 6 0.19809368326440335 1102 7 0.1063737226982866 1102 8 0.1403624602600786 1102 9 0.2345236916938731 1102 10-11 0.3379682222031448 1102 12-13 0.28779875675515143 1102 14-15 0.402283106844898 1102 16-17 0.40080467632211025 1102 18-19 0.41387894604763886 1102 20-21 0.2595135289619108 1102 22-23 0.21733849820857642 1102 24-25 0.293730499400894 1102 26-27 0.2026840936167602 1102 28-29 0.5451265059985175 1102 30-31 0.292083926457245 1102 32-33 0.42345454980888775 1102 34-35 0.35670299765882163 1102 36-37 0.5116159644657472 1102 38-39 0.43651723456957114 1102 40-41 0.6308062532984735 1102 42-43 0.5444993484173111 1102 44-45 0.6508330415944954 1102 46-47 0.5680917452005758 1102 48-49 0.5711316152629777 1102 50-51 0.5211883668845232 1102 52-53 0.664845940278397 1102 54-55 0.8248878824427806 1102 56-57 0.7547788105882063 1102 58-59 0.7950017694174463 1102 60-61 0.9835167131021336 1102 62-63 1.0190022558749448 1102 64-65 0.9453312355153258 1102 66-67 0.804873939019437 1102 68-69 0.6124891267655244 1102 70-71 0.9237731287134991 1102 72-73 0.9222957237440355 1102 74-75 0.9424566705105484 1102 76-77 0.8744566155945925 1102 78-79 1.0120961818729803 1102 80-81 1.022820855376409 1102 82-83 0.9577287843262852 1102 84-85 0.897065717815174 1102 86-87 0.9877750431619496 1102 88-89 0.953274277439327 1102 90-91 0.7543498301391018 1102 92-93 0.8212802971869948 1102 94-95 0.8284130972420378 1102 96-97 0.6210126827529088 1102 98-99 0.6194232864003268 1102 100-101 0.7879391399905451 1103 1 0.22200204022204062 1103 2 0.2525845982282 1103 3 0.20273047716913695 1103 4 0.21947233869990157 1103 5 0.2523203493679418 1103 6 0.2045838093468646 1103 7 0.21994755238481645 1103 8 0.13137972505351314 1103 9 0.2929832266131882 1103 10-11 0.3429086984271166 1103 12-13 0.20422178105710032 1103 14-15 0.3513872251934558 1103 16-17 0.4643501693932848 1103 18-19 0.44038423376699853 1103 20-21 0.32220385843768184 1103 22-23 0.1988445653959019 1103 24-25 0.39997676738141763 1103 26-27 0.43961140274137733 1103 28-29 0.5270284978909245 1103 30-31 0.579879258991788 1103 32-33 0.7161568723983223 1103 34-35 0.7469010050347933 1103 36-37 0.7754340743487873 1103 38-39 0.6132817164935105 1103 40-41 0.7902147693659245 1103 42-43 0.8850899881607219 1103 44-45 1.0404412421603269 1103 46-47 0.964510582324408 1103 48-49 0.9947510705583795 1103 50-51 0.8947364319186093 1103 52-53 1.0873955265413002 1103 54-55 1.2319674213804461 1103 56-57 1.1968435171227298 1103 58-59 1.2619444484122724 1103 60-61 1.123469202137521 1103 62-63 1.2758355448414491 1103 64-65 1.1454240400410782 1103 66-67 1.2517302630935134 1103 68-69 1.304513171523304 1103 70-71 1.3025281523423473 1103 72-73 1.2793179422393166 1103 74-75 1.37269710120459 1103 76-77 1.2410418086296922 1103 78-79 1.207321954629645 1103 80-81 1.2695717120876857 1103 82-83 1.325381876619229 1103 84-85 1.353856741045572 1103 86-87 1.245877992589957 1103 88-89 1.1772393437672655 1103 90-91 1.2064710804620056 1103 92-93 1.1712951830554381 1103 94-95 1.1198015801384855 1103 96-97 1.2336722556044606 1103 98-99 1.163523164105463 1103 100-101 1.398301955597688 1104 1 0.409181602078057 1104 2 0.4374658032758525 1104 3 0.25300794247232616 1104 4 0.29132389931329783 1104 5 0.29492550161891273 1104 6 0.2984293340432771 1104 7 0.23218624171468605 1104 8 0.2516581732864793 1104 9 0.2626270783873821 1104 10-11 0.30673051145930685 1104 12-13 0.28444766074892414 1104 14-15 0.3656642803358139 1104 16-17 0.4703505916890549 1104 18-19 0.6466845103221814 1104 20-21 0.5204459309875986 1104 22-23 0.6163239693764027 1104 24-25 0.522119748473969 1104 26-27 0.5515868246427473 1104 28-29 0.3425595772813139 1104 30-31 0.31252994152124103 1104 32-33 0.5139906650389676 1104 34-35 0.6434983084093986 1104 36-37 0.6227541344895258 1104 38-39 0.5759058113790019 1104 40-41 0.5344834392677669 1104 42-43 0.5022279150525932 1104 44-45 0.4895661312773498 1104 46-47 0.49325521109852843 1104 48-49 0.44548565888675995 1104 50-51 0.29431862608485204 1104 52-53 0.4042890558295724 1104 54-55 0.4574275478021974 1104 56-57 0.44788985194745123 1104 58-59 0.4252281983986208 1104 60-61 0.5224018738741663 1104 62-63 0.3771828867444711 1104 64-65 0.4381684406784032 1104 66-67 0.37109100681944795 1104 68-69 0.47102663462763417 1104 70-71 0.3493614195402799 1104 72-73 0.2813632856397916 1104 74-75 0.12550269832229688 1104 76-77 0.30524562943552525 1104 78-79 0.3974132942901356 1104 80-81 0.40657847246032475 1104 82-83 0.4537995295527928 1104 84-85 0.5315385921571902 1104 86-87 0.5908180884802192 1104 88-89 0.755713848874251 1104 90-91 0.7121863317704573 1104 92-93 0.6847106577680506 1104 94-95 0.5483362138515204 1104 96-97 0.5421699263035649 1104 98-99 0.6948052079198632 1104 100-101 0.7718556229572577 1105 1 0.34009775422183 1105 2 0.31620632012378636 1105 3 0.2679097310314056 1105 4 0.39383666550892826 1105 5 0.14172684842026229 1105 6 0.2531211800394715 1105 7 0.29209909574518633 1105 8 0.3141845452757721 1105 9 0.3037415478905956 1105 10-11 0.43579610344050224 1105 12-13 0.30542038888278 1105 14-15 0.48840705333434187 1105 16-17 0.6005593286752813 1105 18-19 0.5652779468184335 1105 20-21 0.47339830209854483 1105 22-23 0.5374146717970234 1105 24-25 0.6070526236881761 1105 26-27 0.508711352099759 1105 28-29 0.40586742127969444 1105 30-31 0.2835616567984829 1105 32-33 0.4106253839243337 1105 34-35 0.5061034734442273 1105 36-37 0.3484251670659262 1105 38-39 0.4242937831685083 1105 40-41 0.5467760154989563 1105 42-43 0.4314304260965329 1105 44-45 0.18987699782862677 1105 46-47 0.30296785329198883 1105 48-49 0.5068516633473905 1105 50-51 0.5917860539870645 1105 52-53 0.6636172051833 1105 54-55 0.7175436276113771 1105 56-57 0.627811403352375 1105 58-59 0.6970254548506105 1105 60-61 0.4023802596070283 1105 62-63 0.5212643665037007 1105 64-65 0.659088209552138 1105 66-67 0.8659479307045075 1105 68-69 0.8317071826483442 1105 70-71 0.7884432006936706 1105 72-73 0.7477384610942508 1105 74-75 0.8405259056728731 1105 76-77 0.670022985261479 1105 78-79 0.612104968061093 1105 80-81 0.7585702573165598 1105 82-83 0.8453191589240809 1105 84-85 0.7118002884035448 1105 86-87 0.7841258463659813 1105 88-89 0.9703387853661951 1105 90-91 0.9096813157104542 1105 92-93 1.041492457005095 1105 94-95 0.8011133457846604 1105 96-97 0.7119840091611245 1105 98-99 0.7516244661457705 1105 100-101 0.9787045900364042 1106 1 0.49957642172188343 1106 2 0.4840420494568427 1106 3 0.46453218166454846 1106 4 0.4846300076391685 1106 5 0.42704758828645595 1106 6 0.4095556546557617 1106 7 0.6001297460218282 1106 8 0.4240417112826691 1106 9 0.7240253638963381 1106 10-11 0.7143513271828965 1106 12-13 0.5691771312491483 1106 14-15 1.0007574480376817 1106 16-17 0.8876047253463497 1106 18-19 0.9966099837654383 1106 20-21 0.9646997813679334 1106 22-23 1.0435493570760954 1106 24-25 1.0511575383813252 1106 26-27 1.1300797341633313 1106 28-29 1.077997430308102 1106 30-31 0.9633715973036132 1106 32-33 1.028006220556506 1106 34-35 0.7941327347355198 1106 36-37 0.9555969614035007 1106 38-39 1.096759463655573 1106 40-41 1.164972881022031 1106 42-43 1.1737967941313556 1106 44-45 1.2156658257564885 1106 46-47 0.9523416523984025 1106 48-49 0.9712068269004135 1106 50-51 1.0880473684302032 1106 52-53 1.160306790108173 1106 54-55 1.17078690839476 1106 56-57 1.1206754911362538 1106 58-59 1.1467731225127196 1106 60-61 1.08787925259265 1106 62-63 1.1919996027469537 1106 64-65 1.2829336154831026 1106 66-67 1.508666175027031 1106 68-69 1.3987373004805974 1106 70-71 1.507509339545912 1106 72-73 1.2694124199339782 1106 74-75 1.2406752215975878 1106 76-77 1.48402048508785 1106 78-79 1.44497530627903 1106 80-81 1.5452221081395336 1106 82-83 1.366113427044553 1106 84-85 1.284467371765814 1106 86-87 1.4530727639759995 1106 88-89 1.5746052853546217 1106 90-91 1.5023555754970133 1106 92-93 1.4307961123515298 1106 94-95 1.6224782553848662 1106 96-97 1.5542705105385366 1106 98-99 1.6688235771951483 1106 100-101 1.9762622908026621 1107 1 0.07182587621788983 1107 2 0.17756832327821215 1107 3 0.307262606879501 1107 4 0.3002379123475052 1107 5 0.1115824727261483 1107 6 0.15683369788811774 1107 7 0.2674199046970429 1107 8 0.29382542590217753 1107 9 0.3354930014467925 1107 10-11 0.46340527181037316 1107 12-13 0.376790698072611 1107 14-15 0.3575263336023724 1107 16-17 0.5493866844629025 1107 18-19 0.44269626302639864 1107 20-21 0.5826362000182996 1107 22-23 0.5829653432793265 1107 24-25 0.5656064402647871 1107 26-27 0.6219314807033172 1107 28-29 0.6749549706259472 1107 30-31 0.6328598434696246 1107 32-33 0.6075502842985685 1107 34-35 0.5780309402900841 1107 36-37 0.4527387059036485 1107 38-39 0.4931946800336817 1107 40-41 0.4450714117155101 1107 42-43 0.5061128835630981 1107 44-45 0.7485530717803357 1107 46-47 0.6194315353854876 1107 48-49 0.6659761698848925 1107 50-51 0.7309737423894163 1107 52-53 0.6006005277841169 1107 54-55 0.5759093918132976 1107 56-57 0.5765962957005542 1107 58-59 0.7434351943770778 1107 60-61 0.6818858171846571 1107 62-63 0.49823658328189424 1107 64-65 0.6879918128782876 1107 66-67 0.5486508452829995 1107 68-69 0.7544881179639233 1107 70-71 0.6718917919340726 1107 72-73 0.5857423061353657 1107 74-75 0.5892939992977553 1107 76-77 0.5913923611889054 1107 78-79 0.6048367204479099 1107 80-81 0.6437929803896054 1107 82-83 0.7389025132558196 1107 84-85 0.6736877182922036 1107 86-87 0.5292238855816684 1107 88-89 0.5773083890096551 1107 90-91 0.6505558759105448 1107 92-93 0.5574395783572044 1107 94-95 0.7352408854249823 1107 96-97 0.8424536268035183 1107 98-99 1.105451775270275 1107 100-101 1.2187732781494418 1108 1 0.1598632554965853 1108 2 0.3242632190876442 1108 3 0.20042920749275694 1108 4 0.21420403737618443 1108 5 0.09243915072618591 1108 6 0.23736601886598407 1108 7 0.012140498760579987 1108 8 0.060330087882526584 1108 9 0.15367159596922875 1108 10-11 0.0588981693302415 1108 12-13 0.022383197186108816 1108 14-15 0.27188721468537125 1108 16-17 0.2830947840019249 1108 18-19 0.37061808359751325 1108 20-21 0.3534023500777508 1108 22-23 0.3846371899241632 1108 24-25 0.42813016734598364 1108 26-27 0.3592122007550458 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-2.495564923959865 2316 78-79 -2.3865798717284825 2316 80-81 -2.5350528460267725 2316 82-83 -2.4633114440931756 2316 84-85 -2.231753796201474 2316 86-87 -2.3503492851500383 2316 88-89 -2.599098413016481 2316 90-91 -2.5140665648164813 2316 92-93 -2.9068902220088617 2316 94-95 -3.055882002243685 2316 96-97 -3.0003681711788595 2316 98-99 -2.9437719296631073 2316 100-101 -2.9244789672002334 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2785.0 3 748.0 4 834.0 5 810.0 6 901.0 7 1074.0 8 1324.0 9 1643.0 10 1988.0 11 2562.0 12 3293.0 13 4141.0 14 4686.0 15 5636.0 16 7279.0 17 9408.0 18 8432.0 19 5788.0 20 5423.0 21 5553.0 22 6271.0 23 6928.0 24 7943.0 25 8801.0 26 10419.0 27 12285.0 28 14800.0 29 17977.0 30 22056.0 31 27519.0 32 35360.0 33 46242.0 34 64063.0 35 91314.0 36 137319.0 37 196836.0 38 177057.0 39 31762.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.85009968874453 19.122233270650643 14.823023396255424 22.204643644349403 2 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21.146067176967552 20-21 22.28588223212926 26.3220595086758 29.89127493081063 21.50078332838431 22-23 21.8053191354824 26.476091712721157 29.98378713340837 21.734802018388073 24-25 21.982505283113156 26.515866536776432 29.99225109683398 21.50937708327643 26-27 21.707533686083433 26.532879903460703 30.182589142566403 21.576997267889457 28-29 21.148181888537454 26.99642735925551 30.3068485140637 21.54854223814334 30-31 21.40873618929472 26.520599930918028 30.16206954622853 21.908594333558717 32-33 21.327609113101563 26.721956740633452 30.095800951687856 21.85463319457713 34-35 22.01406229930326 26.593108623777866 29.746051299478225 21.646777777440647 36-37 21.826364536563805 26.348858920427364 30.015101110685173 21.809675432323658 38-39 21.59607389167295 26.646376648880025 30.0672068909189 21.69034256852812 40-41 21.68605956868244 26.711673980734563 29.992129536897096 21.610136913685906 42-43 21.52054633775262 26.82024913357598 29.491394431822087 22.167810096849312 44-45 21.651261827977926 26.77903256230755 29.324383574667657 22.245322035046872 46-47 21.994688731555424 27.056624877002474 28.99126350950254 21.957422881939564 48-49 22.03435771324139 27.06963224574195 28.781348467677947 22.114661573338715 50-51 21.66099898780615 27.359518919733333 28.91047089765719 22.06901119480333 52-53 22.088187092275398 27.55933860944958 28.339024601143514 22.01344969713151 54-55 21.667336716452628 27.240785551198126 28.561162903009663 22.530714829339583 56-57 21.99541611500575 27.539823259473984 28.323847631339127 22.140912994181136 58-59 22.348539256286124 27.176144996085398 28.340157757488825 22.13515799013965 60-61 22.002385104325555 27.067191564944025 29.030249758252786 21.900173572477634 62-63 21.86507585279071 27.23462903066734 28.922837266113188 21.977457850428767 64-65 21.671665066268613 27.590811844260283 28.578153782830285 22.159369306640816 66-67 21.74839761746759 27.57610305423822 28.280433128854217 22.395066199439974 68-69 21.492027046846474 27.49609317709413 28.659542509773377 22.352337266286014 70-71 21.899054224240917 27.406566283425338 28.401079297637505 22.293300194696233 72-73 21.771122758910767 27.150899671192324 28.673859587392876 22.404117982504033 74-75 21.47510150012893 27.855073489632577 28.330240718363054 22.33958429187544 76-77 21.824809065537433 27.387761686281394 28.315442998800005 22.471986249381168 78-79 21.720906636912783 27.532321182089607 28.31624356573158 22.430528615266027 80-81 21.77223574861639 27.73507482497857 28.205439380263602 22.287250046141438 82-83 21.650587327879425 27.624009002743815 28.49181281373677 22.233590855639992 84-85 21.60281988343551 27.693954235060087 28.094388607978445 22.60883727352596 86-87 21.782092078314975 27.58151149904071 28.160370988031815 22.4760254346125 88-89 21.79836264126545 27.38756186133953 28.00260852231966 22.811466975075355 90-91 21.68921444507782 27.183278614064005 28.459010622332848 22.668496318525328 92-93 21.92473034511576 27.28029235334528 28.36821992871257 22.426757372826394 94-95 21.536918140071847 27.183927206389374 28.347052624709296 22.932102028829483 96-97 21.292962083521594 27.13649861391008 28.61857001259346 22.95196928997487 98-99 21.41088939783123 27.41612784856575 28.069846009793626 23.10313674380939 100-101 21.28394194460373 27.24774361447832 28.341912819731586 23.126401621186364 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 203.0 1 157.0 2 126.0 3 161.5 4 168.0 5 170.0 6 261.0 7 552.0 8 951.5 9 1135.5 10 1079.5 11 1053.5 12 1117.5 13 1183.0 14 1291.5 15 1391.5 16 1462.0 17 1522.5 18 1615.5 19 1757.0 20 1958.0 21 2316.0 22 2742.0 23 3220.0 24 3840.5 25 4765.5 26 6055.5 27 7555.0 28 8833.5 29 10249.5 30 12129.0 31 13968.5 32 15960.5 33 18054.5 34 20538.5 35 22532.0 36 24588.0 37 27268.0 38 29200.5 39 30007.5 40 30746.5 41 32540.5 42 33793.0 43 35186.0 44 37022.5 45 37885.0 46 37559.0 47 39709.5 48 46973.5 49 46122.0 50 38411.0 51 34704.0 52 31963.5 53 30323.0 54 29002.0 55 26272.5 56 23425.5 57 20905.5 58 18695.0 59 16695.0 60 14113.0 61 11249.5 62 8715.0 63 6582.0 64 4903.5 65 3685.5 66 2657.5 67 2034.0 68 1445.0 69 888.5 70 605.0 71 417.0 72 286.5 73 213.0 74 158.5 75 114.5 76 82.0 77 47.5 78 28.0 79 16.0 80 9.5 81 11.0 82 10.0 83 6.0 84 4.0 85 4.0 86 4.5 87 4.5 88 3.5 89 1.0 90 0.0 91 0.5 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07075874603371958 2 0.11574109172658416 3 0.08167580970749345 4 0.14758252744175795 5 0.12200829494671361 6 0.11543783995786822 7 0.12847766601265367 8 0.11018147596679191 9 0.13322861038920344 10-11 0.12256425652269283 12-13 0.10553161551314749 14-15 0.10836196535449626 16-17 0.12625381970873678 18-19 0.10477348609135764 20-21 0.0875892191974543 22-23 0.08738705135164368 24-25 0.07581294217898525 26-27 0.06711972480912828 28-29 0.08010900890246109 30-31 0.06029656001301961 32-33 0.061913902779504634 34-35 0.0539788148314375 36-37 0.061459025126430714 38-39 0.06201498670240994 40-41 0.07793570455999685 42-43 0.0738923476437843 44-45 0.05787054586329208 46-47 0.044072590386716765 48-49 0.05382718894707953 50-51 0.033964198096185394 52-53 0.048671908878908536 54-55 0.0550401960219433 56-57 0.06024601805156696 58-59 0.06782731226946548 60-61 0.06479479458230607 62-63 0.06919194522868721 64-65 0.04654914649789695 66-67 0.07571185825607996 68-69 0.06287420004710512 70-71 0.03037571883304676 72-73 0.025169896803423106 74-75 0.03659238009172355 76-77 0.05271526579512108 78-79 0.07303313429908913 80-81 0.04745890180404477 82-83 0.06969736484321377 84-85 0.07333638606780507 86-87 0.07899708575050264 88-89 0.09886007660139678 90-91 0.07545914844881665 92-93 0.0893581878482973 94-95 0.09587810087569003 96-97 0.10770491985561172 98-99 0.14045611087693335 100-101 0.13732250926686865 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 989277.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.0721565459547 #Duplication Level Percentage of deduplicated Percentage of total 1 83.16714574523337 53.286983816688235 2 10.28054216726048 13.173930142360039 3 2.8052462864355467 5.392145376433693 4 1.1855348358145543 3.0383909436397127 5 0.6432908963703574 2.0608517508414534 6 0.38388030817617036 1.4757623520223755 7 0.26387005246796474 1.1834706316661703 8 0.19919174980084753 1.0210115980722034 9 0.14027004706586976 0.8088663972881558 >10 0.8356552407182275 9.926502613961073 >50 0.06453715560732345 2.8717605253277054 >100 0.02798916044764231 3.1940447660600793 >500 0.0017394389232521091 0.8143926444141929 >1k 0.0011069156784331605 1.751886441224908 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4418 0.4465887713956758 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3285 0.33206068674395545 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2837 0.2867750892823749 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2062 0.2084350490307568 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1992 0.20135917442738485 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1483 0.14990745766858019 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1214 0.12271588240705081 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0108392290531368E-4 0.0 10-11 0.0 0.0 0.0 1.5162588435797052E-4 0.0 12-13 0.0 1.0108392290531368E-4 0.0 2.527098072632842E-4 0.0 14-15 0.0 1.0108392290531368E-4 0.0 3.0325176871594104E-4 0.0 16-17 0.0 1.0108392290531368E-4 0.0 4.548776530739116E-4 0.0 18-19 5.054196145265684E-5 1.0108392290531368E-4 0.0 5.054196145265684E-4 0.0 20-21 1.0108392290531368E-4 1.0108392290531368E-4 0.0 5.054196145265684E-4 0.0 22-23 1.0108392290531368E-4 1.0108392290531368E-4 0.0 6.065035374318821E-4 0.0 24-25 1.0108392290531368E-4 1.0108392290531368E-4 0.0 0.0010108392290531368 0.0 26-27 1.0108392290531368E-4 1.0108392290531368E-4 0.0 0.0016173427664850188 0.0 28-29 1.0108392290531368E-4 1.0108392290531368E-4 0.0 0.002830349841348783 0.0 30-31 1.0108392290531368E-4 1.0108392290531368E-4 0.0 0.006267203220129448 0.0 32-33 1.0108392290531368E-4 1.0108392290531368E-4 0.0 0.009704056598910113 0.0 34-35 1.0108392290531368E-4 1.0108392290531368E-4 0.0 0.012787116247522181 0.0 36-37 1.0108392290531368E-4 1.0108392290531368E-4 0.0 0.01723480885535598 0.0 38-39 1.0108392290531368E-4 1.5162588435797052E-4 0.0 0.023552554036938088 0.0 40-41 1.0108392290531368E-4 2.0216784581062735E-4 0.0 0.02987029921852019 0.0 42-43 1.5162588435797052E-4 2.0216784581062735E-4 0.0 0.036086960477196985 5.054196145265684E-5 44-45 3.0325176871594104E-4 2.0216784581062735E-4 0.0 0.038967852279998424 1.5162588435797052E-4 46-47 3.0325176871594104E-4 2.0216784581062735E-4 0.0 0.04361771273364285 2.0216784581062735E-4 48-49 3.0325176871594104E-4 2.0216784581062735E-4 0.0 0.05039033556829887 2.0216784581062735E-4 50-51 3.0325176871594104E-4 2.0216784581062735E-4 0.0 0.05675862271133363 2.0216784581062735E-4 52-53 3.0325176871594104E-4 2.0216784581062735E-4 0.0 0.07055657818790895 2.0216784581062735E-4 54-55 3.0325176871594104E-4 3.0325176871594104E-4 0.0 0.07990684105665047 2.0216784581062735E-4 56-57 3.0325176871594104E-4 3.5379373016859787E-4 0.0 0.10790708770142235 2.0216784581062735E-4 58-59 3.0325176871594104E-4 5.054196145265684E-4 0.0 0.15814579738536325 2.0216784581062735E-4 60-61 3.0325176871594104E-4 5.054196145265684E-4 0.0 0.19655768808938245 2.0216784581062735E-4 62-63 4.043356916212547E-4 5.054196145265684E-4 0.0 0.2152582138268655 2.0216784581062735E-4 64-65 4.043356916212547E-4 5.054196145265684E-4 0.0 0.2503343350750093 2.0216784581062735E-4 66-67 4.043356916212547E-4 5.054196145265684E-4 0.0 0.3399957746920226 2.0216784581062735E-4 68-69 4.043356916212547E-4 5.054196145265684E-4 0.0 0.46650230420802263 2.0216784581062735E-4 70-71 4.043356916212547E-4 5.559615759792253E-4 0.0 0.5748642695625189 2.0216784581062735E-4 72-73 4.043356916212547E-4 6.065035374318821E-4 0.0 0.695912267241632 2.0216784581062735E-4 74-75 4.043356916212547E-4 6.065035374318821E-4 0.0 0.8506212112482145 2.0216784581062735E-4 76-77 4.043356916212547E-4 6.065035374318821E-4 0.0 0.9427086650149554 2.0216784581062735E-4 78-79 4.043356916212547E-4 6.065035374318821E-4 0.0 1.0391932694280772 2.0216784581062735E-4 80-81 5.054196145265684E-4 6.065035374318821E-4 0.0 1.1463927696691625 2.0216784581062735E-4 82-83 5.054196145265684E-4 6.065035374318821E-4 0.0 1.262083319434294 2.0216784581062735E-4 84-85 5.559615759792253E-4 6.065035374318821E-4 0.0 1.4292761279196826 2.0216784581062735E-4 86-87 6.065035374318821E-4 6.065035374318821E-4 0.0 1.7384413061255846 2.0216784581062735E-4 88-89 6.065035374318821E-4 6.065035374318821E-4 0.0 2.1552608622256457 2.0216784581062735E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTACCG 65 0.005821455 29.253572 5 GTACATG 4490 0.0 26.466953 1 TACATGG 4555 0.0 25.570074 2 GTATAGG 300 0.0 25.35181 1 ACATGGG 4520 0.0 24.507202 3 GAGTACT 3595 0.0 22.343647 12-13 GTATCAA 8565 0.0 21.422503 1 AGTACTT 3820 0.0 20.903172 12-13 CATGGGA 2850 0.0 20.518068 4 GTACTTT 3970 0.0 20.115417 14-15 ATGGGAG 1225 0.0 19.402878 5 AGAGTAC 6655 0.0 19.320015 10-11 TCAACGC 9710 0.0 18.752356 4 CAACGCA 9670 0.0 18.729698 5 CATGGGG 1905 0.0 18.717272 4 ATCAACG 9785 0.0 18.608622 3 TATCAAC 9800 0.0 18.579197 2 AACGCAG 9815 0.0 18.501432 6 GTATAGC 430 3.6379788E-12 17.68731 1 GTACCTG 700 0.0 17.655724 1 >>END_MODULE