FastQCFastQC Report
Thu 26 May 2016
SRR936915_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936915_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989277
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58080.5870954242340618No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45250.45740475114654444No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37940.3835124035027601No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30000.303251768715941No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14920.150817212974728No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12330.12463647694225177No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11760.11887469333664888No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT10750.1086652171232122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGG308.294536E-539.58202710-11
TTGCGCG700.00839236527.1419649
GTATAGG2750.025.9134771
GTACATG45150.024.5168841
TACATGG44450.024.3636512
ACAGCGC2052.669367E-823.1699688
ACATGGG44850.022.6625443
AGTACTT51150.022.33252312-13
GAGTACT49750.021.95851912-13
GTCTACC1954.2133433E-721.9267861
TATAGGG2205.639231E-821.5901972
GTACACG1553.9006205E-521.4552421
GTATCAA103400.020.6756461
GTACTTT52900.020.51784714-15
ACTGCGT1200.00476394719.7910148
AGAGTAC81300.019.60113710-11
CATGGGA28750.019.328824
TACTTTT59100.019.08874514-15
GTATATG4350.018.566361
TCAACGC118150.017.84874
Produced by FastQC (version 0.11.5)