FastQCFastQC Report
Thu 26 May 2016
SRR936914_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936914_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816390
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37980.46521882923602687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27100.33194919095040365No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23690.29017993850978085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18560.22734232413429856No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16570.2029667193375715No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12980.15899263832237043No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC10240.1254302477982337No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGA600.003941400431.6812362
CTAAGGG1901.1841621E-825.0115032
GTACATG37350.024.301671
TACATGG37200.024.0164222
ACATGGG37050.022.8310133
GAGTACT30400.022.74483512-13
GTATCAA75250.021.4085161
AGTACTT32100.021.0221312-13
GTACTTT32850.020.8308614-15
CGCGGCA600.0047753219.78379236-37
CATGGGG13700.019.7718684
CCCTATA1707.922192E-519.5678222
GTATATA5450.019.1831361
CATGGGA25050.019.160514
AGAGTAC59350.018.9372610-11
CAACGCA84700.018.7394315
TCAACGC85400.018.5858294
TATCAAC85850.018.5437642
ATCAACG85750.018.5099723
AACGCAG86450.018.4139336
Produced by FastQC (version 0.11.5)