##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936914_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 816390 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.90228322247945 33.0 31.0 34.0 30.0 34.0 2 32.03388331557221 33.0 31.0 34.0 30.0 34.0 3 32.05511336493588 34.0 31.0 34.0 30.0 34.0 4 35.62972476389961 37.0 35.0 37.0 33.0 37.0 5 35.62388931760555 37.0 35.0 37.0 33.0 37.0 6 35.694860299611705 37.0 35.0 37.0 33.0 37.0 7 35.64461593111135 37.0 35.0 37.0 33.0 37.0 8 35.58400886831049 37.0 35.0 37.0 33.0 37.0 9 37.25487818322125 39.0 37.0 39.0 34.0 39.0 10-11 37.082814586165924 39.0 37.0 39.0 33.0 39.0 12-13 37.033175320618824 39.0 37.0 39.0 33.0 39.0 14-15 38.21342434375727 40.0 38.0 41.0 33.0 41.0 16-17 38.152676416908584 40.0 38.0 41.0 33.0 41.0 18-19 38.13904751405578 40.0 38.0 41.0 33.0 41.0 20-21 38.06534254461715 40.0 38.0 41.0 33.0 41.0 22-23 37.94965641421379 40.0 38.0 41.0 33.0 41.0 24-25 37.78630556474234 40.0 37.5 41.0 33.0 41.0 26-27 37.88394211100087 40.0 38.0 41.0 33.0 41.0 28-29 37.84449772780167 40.0 38.0 41.0 33.0 41.0 30-31 37.70824605886892 40.0 37.5 41.0 33.0 41.0 32-33 37.504502137458815 40.0 37.0 41.0 32.5 41.0 34-35 37.32768162275383 40.0 37.0 41.0 32.0 41.0 36-37 37.15397052879139 40.0 36.0 41.0 31.0 41.0 38-39 36.990094195176326 39.0 36.0 41.0 31.0 41.0 40-41 36.82202807481718 39.0 36.0 41.0 31.0 41.0 42-43 36.60889648329842 39.0 36.0 41.0 30.0 41.0 44-45 36.3490090520462 39.0 35.5 41.0 30.0 41.0 46-47 35.97847168632639 39.0 35.0 40.0 29.0 41.0 48-49 35.62834429623096 39.0 35.0 40.0 27.5 41.0 50-51 34.99797278261615 38.5 34.0 39.5 26.5 40.5 52-53 35.24574223104153 38.5 35.0 40.0 27.5 40.5 54-55 35.6321953968079 39.0 35.0 40.5 28.0 41.0 56-57 35.52747890101545 39.0 35.0 41.0 27.5 41.0 58-59 35.287347346243834 39.0 35.0 41.0 27.0 41.0 60-61 34.999947941547546 38.0 35.0 40.0 26.0 41.0 62-63 34.70275236100393 38.0 34.0 40.0 26.0 41.0 64-65 34.38548732836022 37.5 34.0 40.0 26.0 41.0 66-67 34.00524871691226 37.0 34.0 40.0 25.0 41.0 68-69 33.62048653217212 36.5 33.5 39.0 23.5 41.0 70-71 33.172484351841646 36.0 33.0 39.0 22.5 41.0 72-73 32.76890640502701 35.5 33.0 39.0 22.0 40.0 74-75 32.34072869584389 35.0 32.0 37.5 20.5 40.0 76-77 31.90124389078749 35.0 32.0 37.0 19.5 39.0 78-79 31.44253604282267 35.0 31.5 36.5 18.0 39.0 80-81 30.987893041316035 35.0 31.0 36.0 15.0 38.5 82-83 30.561487156873554 34.5 31.0 36.0 10.0 37.0 84-85 30.197722902044365 34.0 30.5 35.0 7.0 37.0 86-87 29.875136270654956 34.0 30.0 35.0 4.5 36.5 88-89 29.50220544102696 34.0 30.0 35.0 2.0 36.0 90-91 29.138760273888707 34.0 29.5 35.0 2.0 36.0 92-93 28.75508457967393 34.0 29.0 35.0 2.0 35.0 94-95 28.34478129325445 34.0 29.0 35.0 2.0 35.0 96-97 27.874709391344823 34.0 29.0 35.0 2.0 35.0 98-99 27.259504036061195 34.0 27.0 35.0 2.0 35.0 100-101 24.999917931380836 31.5 22.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20692797924198558 1101 2 0.10992578011449083 1101 3 0.20021669555096366 1101 4 0.13951839256408505 1101 5 0.24314682660850195 1101 6 0.08597012128154802 1101 7 -0.024690427679907145 1101 8 0.07002568339272841 1101 9 -0.027802464132065552 1101 10-11 0.01731655700827872 1101 12-13 0.05504117376899842 1101 14-15 0.1325344095808063 1101 16-17 0.3126087033158669 1101 18-19 0.13141429112606318 1101 20-21 0.19974771643011735 1101 22-23 0.3574728445603341 1101 24-25 0.09426018638740175 1101 26-27 0.21054116460539518 1101 28-29 0.40495453293475236 1101 30-31 0.10394249742532935 1101 32-33 0.24863421300904776 1101 34-35 0.2389710248186887 1101 36-37 0.1327943634863118 1101 38-39 0.28205972871325713 1101 40-41 0.23630412448538607 1101 42-43 0.22715402415454378 1101 44-45 0.33216621712752215 1101 46-47 0.1661002760153636 1101 48-49 0.09894620187489522 1101 50-51 0.08813494275324274 1101 52-53 0.07655095956333469 1101 54-55 0.11819045404325834 1101 56-57 0.06906047383463942 1101 58-59 0.018184380200601424 1101 60-61 0.24491915432575695 1101 62-63 0.31748119059390234 1101 64-65 0.2737228211950864 1101 66-67 0.23712960751917933 1101 68-69 0.08135616656161915 1101 70-71 0.28983157096905643 1101 72-73 0.2625557612577225 1101 74-75 0.14067928277382435 1101 76-77 0.3339533138561279 1101 78-79 0.34880611085396396 1101 80-81 -0.017402832519259448 1101 82-83 0.11405167859006937 1101 84-85 0.2461870747099617 1101 86-87 0.1580839037403905 1101 88-89 0.12723365424100308 1101 90-91 0.25277240624141584 1101 92-93 0.25941417116383647 1101 94-95 0.2193238332841645 1101 96-97 0.23713330739957783 1101 98-99 0.35375747194669316 1101 100-101 0.5414043401024351 1102 1 0.22954843846803286 1102 2 0.1358082516588972 1102 3 0.1523150407818008 1102 4 0.037855942430390144 1102 5 -0.05422556126132605 1102 6 -0.005512642139748891 1102 7 0.019217122234408635 1102 8 0.08315294267830353 1102 9 0.1744170964203846 1102 10-11 0.26029987009904687 1102 12-13 0.21454973565121804 1102 14-15 0.3274048412033608 1102 16-17 0.2343798151844041 1102 18-19 0.13122886768854158 1102 20-21 0.18860850843422838 1102 22-23 0.20381562228743633 1102 24-25 0.13616288288824308 1102 26-27 0.12825156319977538 1102 28-29 0.23652284316916194 1102 30-31 0.29878532555880355 1102 32-33 0.3012230602393373 1102 34-35 0.3000647470651785 1102 36-37 0.19540987808735366 1102 38-39 0.28733739581037554 1102 40-41 0.2586623505651673 1102 42-43 0.2008821033837549 1102 44-45 0.35297684759120784 1102 46-47 0.33291056083536574 1102 48-49 0.567248395272145 1102 50-51 0.5578443140177711 1102 52-53 0.49724193490261825 1102 54-55 0.5176075403629667 1102 56-57 0.6902247890040769 1102 58-59 0.7100760353058035 1102 60-61 0.7575645527041175 1102 62-63 0.7073540705259234 1102 64-65 0.6586666780985979 1102 66-67 0.4414560343795415 1102 68-69 0.466976308947622 1102 70-71 0.6536791613044741 1102 72-73 0.7607255299164777 1102 74-75 0.7247547099078133 1102 76-77 0.7218573449256525 1102 78-79 0.9280497272470711 1102 80-81 0.9051105731752216 1102 82-83 0.7285065456852671 1102 84-85 0.6536025720288237 1102 86-87 0.6654558355525673 1102 88-89 0.7400077088815102 1102 90-91 0.6915526680665103 1102 92-93 1.017330371771589 1102 94-95 0.8551840060099991 1102 96-97 0.8448619242967546 1102 98-99 1.076668847944621 1102 100-101 1.0669531694744698 1103 1 0.25664069295580916 1103 2 0.28395039344760065 1103 3 0.38460308510052243 1103 4 0.21184702230235075 1103 5 0.2608063188219347 1103 6 0.09457330202118186 1103 7 0.11239947850032195 1103 8 0.11174032448797533 1103 9 0.053415792204056345 1103 10-11 0.35609271182631375 1103 12-13 0.33615661775306904 1103 14-15 0.3072254122393616 1103 16-17 0.39453326930486554 1103 18-19 0.39340474666602177 1103 20-21 0.6548566078776474 1103 22-23 0.6462778540711724 1103 24-25 0.4678433649419276 1103 26-27 0.5684149939593013 1103 28-29 0.49812017287860755 1103 30-31 0.5270296834204302 1103 32-33 0.5524345581447889 1103 34-35 0.42092577617807336 1103 36-37 0.2893534833901654 1103 38-39 0.23589817324921825 1103 40-41 0.4163346460269537 1103 42-43 0.6520824477078193 1103 44-45 0.5240919457085198 1103 46-47 0.44100005498281547 1103 48-49 0.6885613839255953 1103 50-51 0.35362052801325206 1103 52-53 0.4070299894853662 1103 54-55 0.4942619996742934 1103 56-57 0.6259185759802506 1103 58-59 0.7454602590781292 1103 60-61 0.694298428252047 1103 62-63 0.8115736824907813 1103 64-65 0.5174476258656213 1103 66-67 0.7341954634530197 1103 68-69 0.7065326564302623 1103 70-71 0.4230744182268822 1103 72-73 0.4366777241612354 1103 74-75 0.25952069682185197 1103 76-77 0.5022101402321226 1103 78-79 0.3543536356535917 1103 80-81 0.5125199041382444 1103 82-83 0.4787357757323285 1103 84-85 0.36415584440749527 1103 86-87 0.708454602500197 1103 88-89 0.6261190366777392 1103 90-91 0.747413977729618 1103 92-93 0.7035859434302729 1103 94-95 0.7262633168692076 1103 96-97 0.8455964868505532 1103 98-99 0.922578015367403 1103 100-101 1.0789651660750224 1104 1 0.33440867645969874 1104 2 0.08700029556281308 1104 3 0.19557244003458152 1104 4 0.3398831023209823 1104 5 0.2594032456222024 1104 6 0.28811568804538723 1104 7 0.2885025513523374 1104 8 0.42204378470641046 1104 9 0.23268751549785094 1104 10-11 0.48679193605027393 1104 12-13 0.4875668193296647 1104 14-15 0.5598303123899271 1104 16-17 0.6721222610046524 1104 18-19 0.535696276415699 1104 20-21 0.6809038707547899 1104 22-23 0.47075852573814103 1104 24-25 0.6819780669236195 1104 26-27 0.32473099664374416 1104 28-29 0.3197082013782122 1104 30-31 0.7292260928048577 1104 32-33 0.649026672421364 1104 34-35 0.49894107958613887 1104 36-37 0.4718860044362643 1104 38-39 0.6739412773269962 1104 40-41 0.3840161501492432 1104 42-43 0.41461057002380386 1104 44-45 0.5309238864004158 1104 46-47 0.5056965947643803 1104 48-49 0.5518495476904945 1104 50-51 0.524152165836071 1104 52-53 0.43573518068234307 1104 54-55 0.6318609711016734 1104 56-57 0.3349921303975876 1104 58-59 0.4099027852357935 1104 60-61 0.5094557045861805 1104 62-63 0.6543116920393217 1104 64-65 0.8131944189653524 1104 66-67 0.495207501716763 1104 68-69 0.46784330413892405 1104 70-71 0.41036113197560553 1104 72-73 0.6618031662075268 1104 74-75 0.5744658604789592 1104 76-77 0.3768372044188055 1104 78-79 0.4172867714787287 1104 80-81 0.32348491250105127 1104 82-83 0.5374123143316218 1104 84-85 0.4126270683671862 1104 86-87 0.32531217057619344 1104 88-89 0.5031362803952071 1104 90-91 0.6852736565006161 1104 92-93 0.8531897099950534 1104 94-95 0.6939191762211188 1104 96-97 0.6240656592286165 1104 98-99 0.6325408782626347 1104 100-101 0.6394277108012396 1105 1 0.2063211893485395 1105 2 0.2672467429105758 1105 3 0.3730586601144381 1105 4 0.23715003178561744 1105 5 0.3124755631522973 1105 6 0.346568433209157 1105 7 0.25479956114148905 1105 8 0.09144451199765768 1105 9 0.2945730413837353 1105 10-11 0.34220938594822314 1105 12-13 0.32293549319003745 1105 14-15 0.40375282659417167 1105 16-17 0.2783639601674608 1105 18-19 0.2892091482825947 1105 20-21 0.3059150092583707 1105 22-23 0.22809455635735532 1105 24-25 0.3794202421778863 1105 26-27 0.22594679082965285 1105 28-29 0.15300511357285274 1105 30-31 -0.11724395628942119 1105 32-33 0.005595631427425474 1105 34-35 0.07215764604163155 1105 36-37 -0.12296843123410639 1105 38-39 -0.2074697270902206 1105 40-41 -0.13053497876461506 1105 42-43 -0.018258729714382582 1105 44-45 0.09144839322991061 1105 46-47 -0.053971308099384885 1105 48-49 0.26160136061454864 1105 50-51 0.130933286815079 1105 52-53 -0.06660231385448867 1105 54-55 -0.04517917843008945 1105 56-57 0.1826237662936947 1105 58-59 0.20409241136396616 1105 60-61 0.16224574061128294 1105 62-63 0.1948181863173133 1105 64-65 0.15551245824926951 1105 66-67 0.3169092316854858 1105 68-69 0.4008383098460797 1105 70-71 0.2521217156756279 1105 72-73 0.4187939032157928 1105 74-75 0.43295117250556814 1105 76-77 0.5331406427157361 1105 78-79 0.46595672807038824 1105 80-81 0.590572437801363 1105 82-83 0.570099579097036 1105 84-85 0.47534426918698003 1105 86-87 0.3565986359152937 1105 88-89 0.5411716180934185 1105 90-91 0.33149356662880436 1105 92-93 0.29708984111115555 1105 94-95 0.41003319630861057 1105 96-97 0.6119427240950159 1105 98-99 0.6439286721600084 1105 100-101 0.7292570901426068 1106 1 0.1465739316668433 1106 2 0.2656956491906506 1106 3 0.2697999520510592 1106 4 0.34910718105754057 1106 5 0.17871201193027986 1106 6 0.3520479379881678 1106 7 0.3655751080014511 1106 8 0.3803225670364654 1106 9 0.47195966015338087 1106 10-11 0.4755822770894156 1106 12-13 0.615843312806426 1106 14-15 0.7798154348435773 1106 16-17 0.663515028228268 1106 18-19 0.5580480731655229 1106 20-21 0.5726639989305795 1106 22-23 0.37218333690773875 1106 24-25 0.4362673779787727 1106 26-27 0.48604365400240823 1106 28-29 0.49001261886504466 1106 30-31 0.6551198900124575 1106 32-33 0.5540804604735499 1106 34-35 0.41015302740028403 1106 36-37 0.41100937362829626 1106 38-39 0.416323973596171 1106 40-41 0.5606126253151871 1106 42-43 0.591292877187918 1106 44-45 0.5551811535095936 1106 46-47 0.4265354594931452 1106 48-49 0.6674663936506704 1106 50-51 0.7824240816062655 1106 52-53 0.548082399725601 1106 54-55 0.6145789843770189 1106 56-57 0.6255117000931705 1106 58-59 0.8577501187217109 1106 60-61 0.7867880460830889 1106 62-63 0.621515857752307 1106 64-65 0.5184748034927011 1106 66-67 0.6517478999772237 1106 68-69 0.5782942082359739 1106 70-71 0.6816829447474078 1106 72-73 0.9124875333395934 1106 74-75 0.9176256224382158 1106 76-77 0.834238211514247 1106 78-79 0.7307017405792067 1106 80-81 0.8030729189098409 1106 82-83 0.9243006182913405 1106 84-85 0.8637142736773242 1106 86-87 0.7460674921600798 1106 88-89 0.7486249505714504 1106 90-91 0.9578692161252427 1106 92-93 1.0230523787979848 1106 94-95 0.9957308035958015 1106 96-97 0.8526053708331034 1106 98-99 0.9301856482328823 1106 100-101 0.8677476924904148 1107 1 0.09167197088252976 1107 2 0.1890569937284603 1107 3 0.24704084840960405 1107 4 0.06738449198191176 1107 5 0.17491649372299634 1107 6 0.010563344150632759 1107 7 0.11294205478016295 1107 8 0.28197522810089026 1107 9 0.38025097667999574 1107 10-11 0.39697583451238216 1107 12-13 0.25136152008933976 1107 14-15 0.14267958050184149 1107 16-17 0.3302587257072602 1107 18-19 0.4453660003363922 1107 20-21 0.46303514738995943 1107 22-23 0.25814272066124033 1107 24-25 0.3243766216762509 1107 26-27 0.2966528976998788 1107 28-29 0.2987521146633654 1107 30-31 0.2533621869312199 1107 32-33 0.4832401243390976 1107 34-35 0.42239392375882545 1107 36-37 0.5842516705470686 1107 38-39 0.16419512205555264 1107 40-41 0.46915604268212974 1107 42-43 0.2649973589806365 1107 44-45 0.29347947283733333 1107 46-47 0.10886038946513565 1107 48-49 0.3303445449111706 1107 50-51 0.17364957180235052 1107 52-53 0.15491713361916482 1107 54-55 0.37757618325657205 1107 56-57 0.31934223230605596 1107 58-59 0.47186776578053724 1107 60-61 0.3912488303968189 1107 62-63 0.34054246839655633 1107 64-65 0.34054062982324496 1107 66-67 0.2624971996971226 1107 68-69 0.2395196984320478 1107 70-71 0.16504428928522685 1107 72-73 0.31204635686900417 1107 74-75 0.3680247821020828 1107 76-77 0.4183278473685945 1107 78-79 0.5467591635483942 1107 80-81 0.6539902858566151 1107 82-83 0.557367845182096 1107 84-85 0.5751988675148603 1107 86-87 0.34031819244019346 1107 88-89 0.2574974995910573 1107 90-91 0.21803028055101237 1107 92-93 0.2772470566691325 1107 94-95 0.5343652573773134 1107 96-97 0.610252429656633 1107 98-99 0.6500946118183144 1107 100-101 0.5601776644792089 1108 1 0.34371722762752555 1108 2 0.31274218582096225 1108 3 0.24657771276369544 1108 4 0.2375728545331981 1108 5 0.3988410243707676 1108 6 0.18792875506695594 1108 7 0.38675157858968845 1108 8 0.25220399846021024 1108 9 0.27287810586064865 1108 10-11 0.19327541178454055 1108 12-13 0.1817917078302358 1108 14-15 0.36162928832230534 1108 16-17 0.20338908751256213 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94-95 -3.061164399532938 2316 96-97 -2.751959239271457 2316 98-99 -3.0039719062407393 2316 100-101 -3.432733590932042 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1710.0 3 529.0 4 567.0 5 587.0 6 687.0 7 770.0 8 944.0 9 1127.0 10 1423.0 11 1836.0 12 2410.0 13 3139.0 14 3484.0 15 4465.0 16 5718.0 17 7914.0 18 7079.0 19 4317.0 20 4230.0 21 4253.0 22 4622.0 23 5190.0 24 5935.0 25 6654.0 26 7849.0 27 9262.0 28 11144.0 29 13399.0 30 16746.0 31 21035.0 32 27182.0 33 35816.0 34 50147.0 35 73097.0 36 113076.0 37 170718.0 38 158333.0 39 28988.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.773490107654695 19.17380994687728 14.914923992230724 22.1377759532373 2 14.723672278999093 20.486238756954144 40.90287478861848 23.88721417542828 3 19.32105413409101 25.270743968526432 30.366819942242923 25.04138195513963 4 12.341973813853855 16.86462321263131 38.90654947769864 31.886853495816197 5 13.423856953395616 37.41576228847111 34.66328204097342 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26.460234612691824 30.41928550383661 21.494423186119004 28-29 21.096806560232967 26.96405458366692 30.462669900147198 21.476468955952914 30-31 21.26544255330357 26.481226375626843 30.439554987403394 21.813776083666188 32-33 21.170640340744136 26.71532748358203 30.326240213834833 21.78779196183901 34-35 21.910688745899957 26.47694941519841 29.990659100027255 21.621702738874376 36-37 21.705148126620408 26.268902076388002 30.297404714057958 21.728545082933636 38-39 21.50369031941935 26.669647505589072 30.226992925611736 21.599669249379843 40-41 21.50967503258494 26.724184396468086 30.251565547182015 21.514575023764955 42-43 21.36439667374489 26.78766000268266 29.703186180986545 22.144757142585902 44-45 21.487805266439402 26.788358809716588 29.551114252361348 22.172721671482662 46-47 21.846491365070385 27.03492128822358 29.25268063469198 21.86590671201406 48-49 21.826206146394885 27.11920047134855 29.04060726548021 22.01398611677636 50-51 21.563629080614863 27.279292014584527 29.14860086642343 22.00847803837718 52-53 21.900136767904314 27.595757438783654 28.598517908755657 21.905587884556375 54-55 21.476975807785266 27.221662132183155 28.923900300978637 22.37746175905294 56-57 21.812797754509084 27.556331768587505 28.56274736940764 22.068123107495772 58-59 22.192325360610052 27.14923590481732 28.549597280494897 22.108841454077726 60-61 21.8704976036225 26.95698036832665 29.356665751894067 21.81585627615678 62-63 21.77015380657491 27.197853690806518 29.128429408846173 21.903563093772398 64-65 21.56313049954858 27.568640613879168 28.82856782352934 22.03966106304291 66-67 21.57034619505194 27.51575938779273 28.494492253995208 22.419402163160125 68-69 21.314491234540146 27.48777393402121 28.92341395012047 22.27432088131818 70-71 21.72607489172981 27.291867025219148 28.684463610803128 22.297594472247916 72-73 21.589434074143043 27.11791107220674 28.905790101397066 22.38686475225315 74-75 21.328960473974178 27.819459060799 28.613765196074226 22.2378152691526 76-77 21.606624441995397 27.365581154866607 28.537796675894505 22.489997727243487 78-79 21.62871059645253 27.39478601844193 28.579909934748642 22.396593450356892 80-81 21.64111348406709 27.66568521860806 28.433260919375453 22.2599403779494 82-83 21.52383647983689 27.584715442372914 28.718400184281208 22.173047893508986 84-85 21.465369484863967 27.649096885563036 28.34650975030353 22.53902387926947 86-87 21.61896593972066 27.5599730458221 28.46937025238912 22.35169076206812 88-89 21.635111327823502 27.329283860834707 28.238393703797176 22.797211107544612 90-91 21.482765126456048 27.115094358114895 28.674906768988006 22.72723374644105 92-93 21.694168042041756 27.216069282146098 28.610068603341826 22.47969407247032 94-95 21.303582094123776 27.079957731595634 28.62422469638728 22.99223547789332 96-97 21.120191845229815 27.092563240243383 28.8307011185866 22.9565437959402 98-99 21.200282872256455 27.428681383867946 28.354573264894917 23.016462478980678 100-101 21.147414694953678 27.316368236131204 28.438508723707685 23.09770834520743 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 111.0 1 89.5 2 89.0 3 118.5 4 113.5 5 126.5 6 228.5 7 444.5 8 794.5 9 1022.5 10 976.5 11 919.5 12 983.0 13 1068.0 14 1125.5 15 1174.0 16 1224.5 17 1297.0 18 1399.0 19 1504.0 20 1652.5 21 1928.5 22 2328.5 23 2792.0 24 3273.0 25 3988.5 26 5095.0 27 6368.0 28 7435.0 29 8639.5 30 10186.5 31 11812.5 32 13488.5 33 15239.0 34 17403.5 35 19067.0 36 20783.0 37 22894.5 38 24182.0 39 24839.5 40 25564.5 41 26975.0 42 28128.0 43 29168.0 44 30612.0 45 31451.5 46 31210.0 47 32880.5 48 39134.0 49 38617.5 50 31696.5 51 28068.5 52 25831.5 53 24589.0 54 23423.5 55 21246.5 56 18928.0 57 16701.0 58 14927.0 59 13298.5 60 11148.0 61 8901.0 62 6841.5 63 5177.5 64 3780.0 65 2836.5 66 2101.5 67 1610.5 68 1113.5 69 687.0 70 467.0 71 315.0 72 217.5 73 158.5 74 125.5 75 98.5 76 74.5 77 42.0 78 25.5 79 19.0 80 14.0 81 8.5 82 4.0 83 5.5 84 4.5 85 1.0 86 2.0 87 3.0 88 3.0 89 1.5 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04348411910973922 2 0.04042185720060266 3 0.02609047146584353 4 0.04728132387706856 5 0.06663481914281165 6 0.07030953343377552 7 0.07116696676833376 8 0.07079949533923738 9 0.07190190962652654 10-11 0.0769240191575105 12-13 0.07459670010656672 14-15 0.0708607405774201 16-17 0.05683558103357464 18-19 0.04875120959345411 20-21 0.055794411984468203 22-23 0.03123507147319296 24-25 0.017454892882078417 26-27 0.01329021668565269 28-29 0.01696493097661657 30-31 0.012065311921998065 32-33 0.007226938105562292 34-35 0.010717916681977977 36-37 0.0054508261982630845 38-39 0.007961880963755068 40-41 0.00759440953465868 42-43 0.004470902387339384 44-45 0.0015923761927510134 46-47 0.003123507147319296 48-49 0.0011024142872891633 50-51 0.0055733166746285475 52-53 0.005328335721897622 54-55 0.006491995247369518 56-57 0.021619569078504146 58-59 0.007961880963755068 60-61 0.01935349526574309 62-63 0.01286150001837357 64-65 0.008941804774678768 66-67 0.015985007165692865 68-69 0.02492681194037164 70-71 0.017761119072992076 72-73 0.01757738335844388 74-75 0.017516138120261152 76-77 0.02455934051127525 78-79 0.038584500055120714 80-81 0.030500128615000188 82-83 0.030132657185903796 84-85 0.019475985742108553 86-87 0.02327319050943789 88-89 0.018618552407550313 90-91 0.016903685738433837 92-93 0.030500128615000188 94-95 0.02174205955486961 96-97 0.038645745293303446 98-99 0.05806048579722927 100-101 0.0659611215228016 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 816390.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.84540093952687 #Duplication Level Percentage of deduplicated Percentage of total 1 84.32493333680353 55.52409044760703 2 9.63702408759694 12.69107429823397 3 2.559344934993077 5.055632801614994 4 1.1059838183111341 2.9129579179730185 5 0.5683423057511272 1.8711363496539073 6 0.37061331529616237 1.4641909403521856 7 0.24615857525603987 1.1345887057705648 8 0.18311662005442997 0.9645909812939956 9 0.13707102228147433 0.8122946777383048 >10 0.7804049198687705 9.486451213646596 >50 0.059806789696366165 2.6960908784631985 >100 0.02421942215418511 2.84599318009642 >500 0.0016767292144352485 0.736155388062703 >1k 0.0013041227223385267 1.8047522194931653 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3798 0.46521882923602687 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2710 0.33194919095040365 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2369 0.29017993850978085 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1856 0.22734232413429856 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1657 0.2029667193375715 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1298 0.15899263832237043 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1024 0.1254302477982337 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.224904763654626E-4 0.0 0.0 0.0 0.0 8 1.224904763654626E-4 0.0 0.0 0.0 0.0 9 1.224904763654626E-4 0.0 0.0 0.0 0.0 10-11 1.224904763654626E-4 0.0 0.0 1.837357145481939E-4 0.0 12-13 1.224904763654626E-4 0.0 0.0 3.674714290963878E-4 0.0 14-15 1.224904763654626E-4 0.0 0.0 4.287166672791191E-4 0.0 16-17 1.224904763654626E-4 0.0 0.0 4.899619054618504E-4 0.0 18-19 1.224904763654626E-4 0.0 0.0 4.899619054618504E-4 6.12452381827313E-5 20-21 1.224904763654626E-4 0.0 0.0 5.512071436445816E-4 1.224904763654626E-4 22-23 1.224904763654626E-4 0.0 0.0 9.186785727409695E-4 1.224904763654626E-4 24-25 1.224904763654626E-4 0.0 0.0 0.0014086404782028199 1.224904763654626E-4 26-27 1.224904763654626E-4 0.0 0.0 0.0021435833363955955 1.224904763654626E-4 28-29 1.224904763654626E-4 0.0 0.0 0.003858450005512071 1.224904763654626E-4 30-31 1.224904763654626E-4 0.0 0.0 0.007288183343745024 1.224904763654626E-4 32-33 1.224904763654626E-4 0.0 0.0 0.01139161430198802 1.224904763654626E-4 34-35 1.224904763654626E-4 6.12452381827313E-5 0.0 0.014515121449307316 2.449809527309252E-4 36-37 1.224904763654626E-4 1.224904763654626E-4 0.0 0.020149683362118594 2.449809527309252E-4 38-39 1.224904763654626E-4 1.224904763654626E-4 0.0 0.027499111944046348 2.449809527309252E-4 40-41 1.224904763654626E-4 1.224904763654626E-4 0.0 0.03564472862234962 2.449809527309252E-4 42-43 1.224904763654626E-4 1.224904763654626E-4 0.0 0.0418917429169882 2.449809527309252E-4 44-45 1.224904763654626E-4 1.224904763654626E-4 0.0 0.04538272149340389 2.449809527309252E-4 46-47 1.224904763654626E-4 1.224904763654626E-4 0.0 0.04936366197528142 2.449809527309252E-4 48-49 1.224904763654626E-4 1.224904763654626E-4 0.0 0.05591690246083367 2.449809527309252E-4 50-51 1.224904763654626E-4 1.224904763654626E-4 0.0 0.06308259532821323 2.449809527309252E-4 52-53 1.224904763654626E-4 1.224904763654626E-4 0.0 0.07851639535026152 2.449809527309252E-4 54-55 1.224904763654626E-4 2.449809527309252E-4 0.0 0.08849936917404672 2.449809527309252E-4 56-57 1.224904763654626E-4 2.449809527309252E-4 0.0 0.12126557160180795 2.449809527309252E-4 58-59 1.224904763654626E-4 2.449809527309252E-4 0.0 0.1753451169171597 2.449809527309252E-4 60-61 2.449809527309252E-4 2.449809527309252E-4 0.0 0.22011538602873626 2.449809527309252E-4 62-63 2.449809527309252E-4 2.449809527309252E-4 0.0 0.24240865272725043 2.449809527309252E-4 64-65 2.449809527309252E-4 2.449809527309252E-4 0.0 0.2863827337424515 2.449809527309252E-4 66-67 2.449809527309252E-4 2.449809527309252E-4 0.0 0.38915224341307464 2.449809527309252E-4 68-69 2.449809527309252E-4 2.449809527309252E-4 0.0 0.5438577150626538 2.449809527309252E-4 70-71 2.449809527309252E-4 2.449809527309252E-4 0.0 0.6654907580935583 2.449809527309252E-4 72-73 2.449809527309252E-4 2.449809527309252E-4 0.0 0.8106419725866314 2.449809527309252E-4 74-75 2.449809527309252E-4 2.449809527309252E-4 0.0 0.985558372836512 2.449809527309252E-4 76-77 2.449809527309252E-4 2.449809527309252E-4 0.0 1.0940849348963118 2.449809527309252E-4 78-79 2.449809527309252E-4 2.449809527309252E-4 0.0 1.205061306483421 2.449809527309252E-4 80-81 2.449809527309252E-4 2.449809527309252E-4 0.0 1.340658263819988 2.449809527309252E-4 82-83 2.449809527309252E-4 2.449809527309252E-4 0.0 1.479868690209336 2.449809527309252E-4 84-85 3.0622619091365646E-4 2.449809527309252E-4 0.0 1.6711988142921888 2.449809527309252E-4 86-87 3.674714290963878E-4 2.449809527309252E-4 0.0 2.0210928600301328 2.449809527309252E-4 88-89 3.674714290963878E-4 2.449809527309252E-4 0.0 2.4950085130881074 2.449809527309252E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATCGA 60 0.0039414004 31.681236 2 CTAAGGG 190 1.1841621E-8 25.011503 2 GTACATG 3735 0.0 24.30167 1 TACATGG 3720 0.0 24.016422 2 ACATGGG 3705 0.0 22.831013 3 GAGTACT 3040 0.0 22.744835 12-13 GTATCAA 7525 0.0 21.408516 1 AGTACTT 3210 0.0 21.02213 12-13 GTACTTT 3285 0.0 20.83086 14-15 CGCGGCA 60 0.00477532 19.783792 36-37 CATGGGG 1370 0.0 19.771868 4 CCCTATA 170 7.922192E-5 19.567822 2 GTATATA 545 0.0 19.183136 1 CATGGGA 2505 0.0 19.16051 4 AGAGTAC 5935 0.0 18.93726 10-11 CAACGCA 8470 0.0 18.739431 5 TCAACGC 8540 0.0 18.585829 4 TATCAAC 8585 0.0 18.543764 2 ATCAACG 8575 0.0 18.509972 3 AACGCAG 8645 0.0 18.413933 6 >>END_MODULE