FastQCFastQC Report
Thu 26 May 2016
SRR936914_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936914_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816390
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49410.6052254437217506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39350.48200002449809526No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33610.41169049106431976No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25310.3100233956809858No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13280.16266735261333432No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10130.1240828525582136No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9350.11452859540170753No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT9030.11060890015801271No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8580.10509682872156689No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC8380.10264701919425764No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAA458.9478353E-426.3895678-79
GAGTACT46700.023.28931812-13
GTACTTT49400.022.11391414-15
GTATCAA94100.021.2590331
AGTACTT48550.020.78776712-13
ACTTTTT55150.020.06666416-17
GTCCTAC1951.02882095E-519.4942441
GTACATG37400.019.4388641
GGTATCA73350.018.7218511
TATCAAC106600.018.7147242
ATCAACG106600.018.6667353
TACTTTT53800.018.62794714-15
CAACGCA106650.018.6134535
TCAACGC106700.018.6047334
CTATAAG4650.018.3870032
AGAGTAC77950.018.3384210-11
AACGCAG108400.018.3129626
TACATGG39250.018.0317542
TGCGTAA1608.278221E-917.8124160-61
GCACCCG1600.001192122617.8091359
Produced by FastQC (version 0.11.5)