FastQCFastQC Report
Thu 26 May 2016
SRR936913_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936913_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855460
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38260.4472447572066491No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28790.3365440815467702No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24860.2906038856287845No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18500.21625791971570849No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18120.21181586514857506No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13560.15851121034297339No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC10240.11970168096696514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG37800.025.2754081
TACATGG37550.025.0653972
ACATGGG37800.024.2708423
GAGTACT32750.023.71834812-13
GTGTAGC4750.023.015911
GTATCAA76600.022.2150961
CATGGGA24650.022.176874
GTACTTT35650.021.5865214-15
AGTACTT34350.021.3687812-13
GTCCTAC1805.135342E-621.1257151
ATCAACG85450.019.7485683
CAACGCA85800.019.7234125
TATCAAC85900.019.700452
TCAACGC85850.019.6011834
AACGCAG87150.019.4690136
CATGGGG14600.018.8840224
AGAGTAC62150.018.57832710-11
GTTCGGT901.2382974E-418.46339236-37
ACTTTTT42700.018.41130616-17
GTAAGAC1559.573426E-418.4008923
Produced by FastQC (version 0.11.5)