##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936913_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 855460 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.875912374628854 33.0 31.0 34.0 30.0 34.0 2 32.00909919809225 33.0 31.0 34.0 30.0 34.0 3 32.01870455661282 34.0 31.0 34.0 30.0 34.0 4 35.581997989385826 37.0 35.0 37.0 33.0 37.0 5 35.577586327823624 37.0 35.0 37.0 33.0 37.0 6 35.67779323404952 37.0 35.0 37.0 33.0 37.0 7 35.61807097935613 37.0 35.0 37.0 33.0 37.0 8 35.57856942463704 37.0 35.0 37.0 33.0 37.0 9 37.206159259345846 39.0 37.0 39.0 34.0 39.0 10-11 37.07967175554673 39.0 37.0 39.0 33.0 39.0 12-13 36.9962476328525 39.0 37.0 39.0 33.0 39.0 14-15 38.16804175531293 40.0 38.0 41.0 33.0 41.0 16-17 38.099310312580364 40.0 38.0 41.0 33.0 41.0 18-19 38.056541509830964 40.0 38.0 41.0 33.0 41.0 20-21 37.95465539008253 40.0 38.0 41.0 33.0 41.0 22-23 37.811334837397425 40.0 37.5 41.0 33.0 41.0 24-25 37.93947642204194 40.0 38.0 41.0 33.0 41.0 26-27 37.88995628083136 40.0 38.0 41.0 33.0 41.0 28-29 37.7970290837678 40.0 38.0 41.0 33.0 41.0 30-31 37.62057431089706 40.0 37.0 41.0 32.5 41.0 32-33 37.41069366188951 40.0 37.0 41.0 32.0 41.0 34-35 37.214583382040075 40.0 36.5 41.0 31.5 41.0 36-37 37.0169873518341 39.0 36.0 41.0 31.0 41.0 38-39 36.854730788114 39.0 36.0 41.0 31.0 41.0 40-41 36.66751747597783 39.0 36.0 41.0 30.0 41.0 42-43 36.42762139667548 39.0 35.0 41.0 30.0 41.0 44-45 36.153033455684664 39.0 35.0 40.0 30.0 41.0 46-47 35.796499544104925 39.0 35.0 40.0 28.5 41.0 48-49 35.52954083183317 39.0 35.0 40.0 27.0 41.0 50-51 34.85162076543614 38.0 34.0 39.5 26.5 40.5 52-53 35.057885815818395 38.5 34.5 40.0 27.0 40.5 54-55 35.45519720384355 39.0 35.0 40.0 27.0 41.0 56-57 35.354916068547915 39.0 35.0 41.0 27.0 41.0 58-59 35.10633401912421 38.5 35.0 40.0 26.0 41.0 60-61 34.82330617445585 38.0 34.0 40.0 26.0 41.0 62-63 34.488640614406286 38.0 34.0 40.0 25.5 41.0 64-65 34.14653344399504 37.5 34.0 40.0 24.5 41.0 66-67 33.76838776798448 37.0 33.5 39.5 23.0 41.0 68-69 33.365128118205405 36.0 33.0 39.0 22.0 41.0 70-71 32.928775746382065 36.0 32.5 39.0 21.5 40.5 72-73 32.5058524068922 35.0 32.0 38.5 20.5 40.0 74-75 32.06017756528651 35.0 32.0 37.5 19.5 39.5 76-77 31.606457929067403 35.0 31.0 37.0 18.0 39.0 78-79 31.15430294812148 35.0 31.0 36.5 15.0 39.0 80-81 30.683070511771447 35.0 31.0 36.0 9.5 38.5 82-83 30.240693895681854 34.0 30.0 35.5 7.0 37.0 84-85 29.840569401257802 34.0 30.0 35.0 4.5 37.0 86-87 29.485057162228507 34.0 29.5 35.0 2.0 36.0 88-89 29.108278586959063 34.0 29.0 35.0 2.0 36.0 90-91 28.681195497159422 34.0 29.0 35.0 2.0 36.0 92-93 28.2897441142777 34.0 29.0 35.0 2.0 35.0 94-95 27.852841745961236 34.0 28.0 35.0 2.0 35.0 96-97 27.30966789797302 34.0 27.0 35.0 2.0 35.0 98-99 26.616817267902647 33.5 25.5 35.0 2.0 35.0 100-101 24.015112337222078 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09162202355330962 1101 2 0.11322277721057716 1101 3 0.029033718323947966 1101 4 0.1049263485577967 1101 5 0.1676810634385788 1101 6 0.06898365505153947 1101 7 -0.09680370530065119 1101 8 -0.011961819879552138 1101 9 -0.013405131405335169 1101 10-11 0.18967668151383776 1101 12-13 0.37418326222878306 1101 14-15 0.21542091685619624 1101 16-17 0.13085248814810768 1101 18-19 0.2791351577462535 1101 20-21 0.33758195684111314 1101 22-23 0.27010965882951865 1101 24-25 0.20335323416323092 1101 26-27 0.35447347042688193 1101 28-29 0.19025209971137969 1101 30-31 0.09147699080197214 1101 32-33 0.15239731062952444 1101 34-35 0.17933572216557536 1101 36-37 0.20525682542038481 1101 38-39 0.36952651632302036 1101 40-41 0.2778080573139903 1101 42-43 0.44414400675174903 1101 44-45 0.34068877323812785 1101 46-47 0.551829188456125 1101 48-49 0.6823254470185063 1101 50-51 0.620587152740363 1101 52-53 0.4770999894133041 1101 54-55 0.5247260127007891 1101 56-57 0.45006953614565504 1101 58-59 0.3819396473296379 1101 60-61 0.43259074260723906 1101 62-63 0.5525454242425027 1101 64-65 0.7142228215949586 1101 66-67 0.46785174239433047 1101 68-69 0.5841752333833554 1101 70-71 0.2616926919292979 1101 72-73 0.2159092046815445 1101 74-75 0.3343055412903482 1101 76-77 0.5488658894796288 1101 78-79 0.5433034544077557 1101 80-81 0.4427646988214029 1101 82-83 0.45213171726300416 1101 84-85 0.5543764357269616 1101 86-87 0.5593100105939044 1101 88-89 0.3595385295174047 1101 90-91 0.34329544350146435 1101 92-93 0.31008052049647006 1101 94-95 0.24505951101708234 1101 96-97 0.3044576451895047 1101 98-99 -0.23447889217101547 1101 100-101 -0.0040976931541578665 1102 1 -0.049439658580148205 1102 2 0.14649089012112526 1102 3 -0.12936400796840175 1102 4 -0.14928598461330012 1102 5 -0.0283545983506599 1102 6 -0.1551258175908785 1102 7 -0.07855772229811464 1102 8 0.10020482752484128 1102 9 0.2533054315358996 1102 10-11 0.09935060110389315 1102 12-13 -0.14667981232271643 1102 14-15 -0.16095192692244353 1102 16-17 0.1391294318604963 1102 18-19 0.051755846727850496 1102 20-21 -0.12235102673277964 1102 22-23 0.016593209121140262 1102 24-25 -0.1572800279268236 1102 26-27 -0.15064758073967965 1102 28-29 -0.2276255127002571 1102 30-31 -0.14963238580039473 1102 32-33 -0.08706717652471241 1102 34-35 -0.20655485402593143 1102 36-37 -0.3459003758746988 1102 38-39 -0.31770194669423546 1102 40-41 -0.357881892826569 1102 42-43 -0.2587963653953338 1102 44-45 -0.46205186814667343 1102 46-47 -0.5629004262279125 1102 48-49 -0.39505068979462266 1102 50-51 -0.3807858945129752 1102 52-53 -0.2491541065323375 1102 54-55 -0.18610877072887888 1102 56-57 -0.039276935684490866 1102 58-59 -0.04070863282083792 1102 60-61 -0.46580335369652204 1102 62-63 -0.3874095997105371 1102 64-65 -0.2273685458689485 1102 66-67 -0.2806645201173268 1102 68-69 -0.22126877282180857 1102 70-71 -0.46935648871880176 1102 72-73 -0.6917987423889898 1102 74-75 -0.6792230843438816 1102 76-77 -0.502758896668464 1102 78-79 -0.5195315666803602 1102 80-81 -0.5631942611461689 1102 82-83 -0.41696120881708154 1102 84-85 -0.2751581606687665 1102 86-87 -0.32050725285048287 1102 88-89 -0.3688785024541694 1102 90-91 -0.4176987013106661 1102 92-93 -0.6686588153946751 1102 94-95 -0.4953893867197898 1102 96-97 -0.4164259396114218 1102 98-99 -0.7608410738755502 1102 100-101 -0.6597871712334218 1103 1 0.06913089837967235 1103 2 0.1547816597541818 1103 3 0.13916645485736368 1103 4 0.29662877177125324 1103 5 0.2806892437133115 1103 6 0.20107657062492734 1103 7 0.11993961928647678 1103 8 0.15791161737928405 1103 9 0.2535172331570976 1103 10-11 0.1441117795227882 1103 12-13 0.4708748813058037 1103 14-15 0.3545627597331773 1103 16-17 0.4462151984428999 1103 18-19 0.3265074374190391 1103 20-21 0.32474432757734206 1103 22-23 0.40446604098725913 1103 24-25 0.03516832908529466 1103 26-27 0.2952336185978979 1103 28-29 0.27503987560271526 1103 30-31 0.3699435751514031 1103 32-33 0.3643003820911659 1103 34-35 0.3360512646875762 1103 36-37 0.370746098456344 1103 38-39 0.331662648442169 1103 40-41 0.22992643978042082 1103 42-43 0.29820790550154896 1103 44-45 0.2290681527720082 1103 46-47 0.33162236641568654 1103 48-49 0.30214795049126764 1103 50-51 0.19855211646933668 1103 52-53 0.42352304852737177 1103 54-55 0.5141418176089587 1103 56-57 0.5872897861842503 1103 58-59 0.5122552824585185 1103 60-61 0.7391735485958222 1103 62-63 0.7526827911201153 1103 64-65 0.6321708073952337 1103 66-67 0.4937453988227958 1103 68-69 0.6338109796404936 1103 70-71 0.7860544761552575 1103 72-73 0.4479349802641863 1103 74-75 0.43596474795804596 1103 76-77 0.3194323349187407 1103 78-79 0.42034466447103824 1103 80-81 0.280318333641592 1103 82-83 0.5326557178803846 1103 84-85 0.7048649370388915 1103 86-87 0.7480598176628952 1103 88-89 0.6525544270324559 1103 90-91 0.5068885905920588 1103 92-93 0.3034653020985729 1103 94-95 0.3377667189195357 1103 96-97 0.15706530069190094 1103 98-99 0.265422327152951 1103 100-101 0.6943874125720306 1104 1 0.27089550577963806 1104 2 0.30677898103327195 1104 3 0.23236082954344894 1104 4 0.14402873828723983 1104 5 0.2116016830235452 1104 6 0.09280434561237172 1104 7 0.16006016172745774 1104 8 0.2606121028974471 1104 9 0.32267343607020393 1104 10-11 0.2998886085869117 1104 12-13 0.3120426362028397 1104 14-15 0.35339081933472016 1104 16-17 0.29906217651087275 1104 18-19 0.27762496629900113 1104 20-21 0.4054366095127122 1104 22-23 0.5414047652464262 1104 24-25 0.43363476136387646 1104 26-27 0.14914371402732485 1104 28-29 0.2634744980704866 1104 30-31 0.19066264429589097 1104 32-33 0.42715991666341324 1104 34-35 0.45578674099763106 1104 36-37 0.3656255560153596 1104 38-39 0.38487543933452173 1104 40-41 0.3950151724105879 1104 42-43 0.5807694323724704 1104 44-45 0.47191596794026225 1104 46-47 0.3711150836432182 1104 48-49 0.5282554341678107 1104 50-51 0.3695725417162734 1104 52-53 0.3826934821453136 1104 54-55 0.5957214258460297 1104 56-57 0.6208407655915806 1104 58-59 0.8058422571780852 1104 60-61 0.5895083887096675 1104 62-63 0.5488019942006375 1104 64-65 0.7677400355887727 1104 66-67 0.5525006306995692 1104 68-69 0.6050013362452162 1104 70-71 0.5645024922275752 1104 72-73 0.5016910492412521 1104 74-75 0.467975462841693 1104 76-77 0.31131246215798214 1104 78-79 0.538635305689855 1104 80-81 0.39000023553343866 1104 82-83 0.478209878268796 1104 84-85 0.26207153338913614 1104 86-87 0.24874289149514084 1104 88-89 0.14888792778584659 1104 90-91 0.22331417237675666 1104 92-93 0.007813200976855228 1104 94-95 0.08189864592439378 1104 96-97 0.23212557191239114 1104 98-99 0.19852629500830332 1104 100-101 0.6125700678541364 1105 1 -0.037990313741541826 1105 2 0.10445969005720812 1105 3 -0.02636384817293802 1105 4 0.20263255965004134 1105 5 0.15851121341028573 1105 6 -0.04875297482961827 1105 7 0.037344922316776774 1105 8 -0.04383080686314145 1105 9 0.06397177861163783 1105 10-11 8.715831067007684E-5 1105 12-13 0.15741844191185805 1105 14-15 0.11682601730893083 1105 16-17 0.0909216378368427 1105 18-19 0.013628649540251558 1105 20-21 -0.003570066362051705 1105 22-23 -0.05162297416425332 1105 24-25 -0.03322182242986571 1105 26-27 -0.0235542601899823 1105 28-29 -0.13704345773061988 1105 30-31 -0.3206719551516244 1105 32-33 -0.04647613306034515 1105 34-35 -0.3662119928881893 1105 36-37 -0.08770087124062798 1105 38-39 -0.30543015458813727 1105 40-41 0.03238856099254406 1105 42-43 -0.16052315510450654 1105 44-45 -0.34194510480374873 1105 46-47 -0.18203293661461828 1105 48-49 -0.039490307084484755 1105 50-51 -0.3865709895912701 1105 52-53 -0.31856975450293135 1105 54-55 -0.21090553087589115 1105 56-57 -0.08681059260363355 1105 58-59 -0.22070873269300506 1105 60-61 -0.34974690594963675 1105 62-63 -0.37767705736474255 1105 64-65 -0.20138979866537454 1105 66-67 -0.2072110405711456 1105 68-69 -0.03168144460192224 1105 70-71 -0.22546067535989778 1105 72-73 -0.4986369894327751 1105 74-75 -0.42951820658741013 1105 76-77 -0.7245070590206524 1105 78-79 -0.5743049567410843 1105 80-81 -0.5924468178735616 1105 82-83 -0.5676378427256772 1105 84-85 -0.5438187750823182 1105 86-87 -0.5921295762764984 1105 88-89 -0.47746451236148246 1105 90-91 -0.37969284880187715 1105 92-93 -0.4598490918408231 1105 94-95 -0.4530950235047051 1105 96-97 -0.6797528811262836 1105 98-99 -0.6949848698167465 1105 100-101 -0.669192132882511 1106 1 0.07427794202841653 1106 2 0.31678351714763764 1106 3 0.2888981646912292 1106 4 0.274375134375731 1106 5 0.30409033751168124 1106 6 0.2641691307322489 1106 7 0.17523628629709975 1106 8 0.31128175462002616 1106 9 0.35662217588746614 1106 10-11 0.2879034418899309 1106 12-13 0.40315895931632895 1106 14-15 0.4312834283948277 1106 16-17 0.26217175607369825 1106 18-19 0.3403785549507745 1106 20-21 0.5305873778301518 1106 22-23 0.4090212984426316 1106 24-25 0.4875653985311672 1106 26-27 0.38574388585617925 1106 28-29 0.2702044613007857 1106 30-31 0.40504160425057023 1106 32-33 0.5265457644046521 1106 34-35 0.47324660056622037 1106 36-37 0.4985765597090577 1106 38-39 0.34851127087535616 1106 40-41 0.3953402257674128 1106 42-43 0.29003846994278604 1106 44-45 0.4618367009094513 1106 46-47 0.4847933498023309 1106 48-49 0.43244203778876056 1106 50-51 0.40055242603490626 1106 52-53 0.6048381862840486 1106 54-55 0.1336005937930409 1106 56-57 0.31459347001460713 1106 58-59 0.24242915512387953 1106 60-61 0.3063080032444603 1106 62-63 0.24094406446651107 1106 64-65 0.09892707609814977 1106 66-67 0.2549738582181078 1106 68-69 0.35957612255660365 1106 70-71 0.30441068294506124 1106 72-73 0.2445963155237152 1106 74-75 0.48556231270885064 1106 76-77 0.3901164748423831 1106 78-79 0.41426686032285787 1106 80-81 0.40522391614081954 1106 82-83 0.4749232857268417 1106 84-85 0.47486442109626736 1106 86-87 0.40275076802921816 1106 88-89 0.4095660873358291 1106 90-91 0.2674984347773872 1106 92-93 0.26352515144332145 1106 94-95 0.3581877281033137 1106 96-97 0.5246171529259307 1106 98-99 0.20310264910115094 1106 100-101 -0.17021015217876467 1107 1 0.1324861415791858 1107 2 0.2486071223894335 1107 3 0.22521661159717254 1107 4 0.2219862396648722 1107 5 0.12552896461532725 1107 6 0.09641179157750202 1107 7 0.03140892862007405 1107 8 0.05987238186507682 1107 9 0.24158579418450188 1107 10-11 0.20643506802516498 1107 12-13 0.18100077876762555 1107 14-15 0.24079999302269783 1107 16-17 0.33542189274970724 1107 18-19 0.23828567586579652 1107 20-21 0.33654279051595637 1107 22-23 0.3159793385906582 1107 24-25 0.5462634853194857 1107 26-27 0.2876155581266673 1107 28-29 0.3211577843902589 1107 30-31 0.3188934184115979 1107 32-33 0.17745439159668308 1107 34-35 0.20380735731574617 1107 36-37 0.35245253995699954 1107 38-39 -0.050062500713458746 1107 40-41 0.1819413047819296 1107 42-43 0.24542906032004197 1107 44-45 0.3129532776582451 1107 46-47 0.2556814219319179 1107 48-49 0.26465376503267635 1107 50-51 0.08496277477654246 1107 52-53 0.16605262684671374 1107 54-55 0.1344092865138009 1107 56-57 0.3930032051345975 1107 58-59 0.15939705614645305 1107 60-61 0.2161075156298935 1107 62-63 0.20697139461895375 1107 64-65 0.001618173155172542 1107 66-67 0.01610711385970376 1107 68-69 0.06315772855342772 1107 70-71 0.09713850891893117 1107 72-73 0.12481383555795134 1107 74-75 0.07357724669401478 1107 76-77 0.09716516938127384 1107 78-79 -0.06323304813893316 1107 80-81 0.017687127250653134 1107 82-83 -0.07077385032671302 1107 84-85 -0.09221919684724256 1107 86-87 0.06727207370496657 1107 88-89 -0.10760332400404948 1107 90-91 0.03252616769793448 1107 92-93 0.15145397638313085 1107 94-95 0.23412631038480214 1107 96-97 -0.0674614461985854 1107 98-99 -0.2613508551708428 1107 100-101 -0.29604488594146616 1108 1 -0.13493509652676394 1108 2 -0.09575980852175903 1108 3 -0.09945053107996316 1108 4 0.23051649184655076 1108 5 0.17305775218413544 1108 6 0.050664656108267536 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-2.6795032350896832 2316 100-101 -2.680178631140354 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1659.0 3 613.0 4 613.0 5 584.0 6 709.0 7 866.0 8 1025.0 9 1275.0 10 1604.0 11 2014.0 12 2722.0 13 3472.0 14 3874.0 15 4741.0 16 6334.0 17 8302.0 18 7510.0 19 4718.0 20 4584.0 21 4990.0 22 5293.0 23 5858.0 24 6569.0 25 7550.0 26 8806.0 27 10372.0 28 12617.0 29 15243.0 30 18671.0 31 23538.0 32 29900.0 33 40416.0 34 55339.0 35 79951.0 36 121414.0 37 174317.0 38 152237.0 39 25155.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.786897839536124 19.042529930440008 14.932772706385277 22.23779952363859 2 14.739620841555915 20.603178982613333 40.789344469281055 23.867855706549694 3 19.31655567818328 25.232070371686717 30.367548027460295 25.08382592266971 4 12.302720348713487 16.821356830336576 38.89574841743661 31.980174403513328 5 13.419964559952746 37.459428163726 34.51310872374893 14.607498552572329 6 30.38409316566072 36.50995989844213 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21.08089625605312 27.02258174814936 30.398884794215892 21.49763720158163 30-31 21.282000327309284 26.509304935356415 30.332218923152453 21.876475814181845 32-33 21.175736201357054 26.769656633220546 30.24698537796531 21.80762178745709 34-35 21.861103967456106 26.437179996726908 29.96715217543778 21.73456386037921 36-37 21.812650503822507 26.232903934725176 30.213744652000095 21.740700909452222 38-39 21.51552558334181 26.65083890636489 30.23628251032487 21.59735299996844 40-41 21.52672246510649 26.700371729829563 30.209536389778595 21.563369415285344 42-43 21.3947466859935 26.78190681037103 29.692153928880366 22.1311925747551 44-45 21.60312674065809 26.797118975240792 29.4759132372719 22.123841046829217 46-47 21.865955158628108 27.08420031328174 29.155074462862085 21.894770065228066 48-49 21.949066900616103 27.10620248510881 28.90506870316457 22.039661911110514 50-51 21.564596825099947 27.300867369602322 29.126727140953406 22.00780866434433 52-53 22.0215102030558 27.547753854822204 28.493917560782016 21.93681838133998 54-55 21.48768397470105 27.199820445484573 28.875253448329275 22.437242131485107 56-57 21.917661698375785 27.516881298157653 28.51161102496497 22.053845978501588 58-59 22.245627611553402 27.194932278610118 28.50832920784122 22.05111090199526 60-61 21.89962557149153 27.05418400186104 29.24423423349703 21.801956193150403 62-63 21.80123983994071 27.202013483230132 29.077613325649114 21.919133351180044 64-65 21.638527513557175 27.554237472397602 28.718418705454784 22.088816308590438 66-67 21.572896962441458 27.614577755746716 28.422543543171397 22.38998173864043 68-69 21.430905525044356 27.426585567600902 28.863336772541672 22.27917213481307 70-71 21.762200248114876 27.423884739999693 28.485856165865147 22.328058846020284 72-73 21.66220331264465 27.105156854595208 28.864425694655143 22.368214138105 74-75 21.389111359238626 27.73078294623493 28.537639060207297 22.342466634319152 76-77 21.694776716728224 27.38065238858931 28.469486851021507 22.455084043660957 78-79 21.661025272277662 27.425653437158378 28.575737911924094 22.33758337863987 80-81 21.661973890034066 27.599783635220675 28.409514202593456 22.328728272151807 82-83 21.573878307076303 27.517260412378853 28.71841438087041 22.190446899674438 84-85 21.51557596703816 27.554799998129294 28.380073705822106 22.549550329010444 86-87 21.61061286811428 27.490386852464344 28.448135768391246 22.45086451103013 88-89 21.706471753557437 27.270415634201218 28.21540283542924 22.807709776812096 90-91 21.542986528508315 27.16853132503284 28.61155883568615 22.676923310772693 92-93 21.807981019455877 27.21793890608127 28.564245552019635 22.40983452244322 94-95 21.452504822587244 27.153270590986146 28.470216870287018 22.92400771613959 96-97 21.12799317182677 27.10808156393228 28.7712211205687 22.99270414367225 98-99 21.299990002145737 27.363600911383344 28.252532935913756 23.083876150557163 100-101 21.214355589521873 27.238302622338672 28.410276980758294 23.137064807381165 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 96.0 1 90.5 2 99.5 3 123.0 4 122.0 5 130.5 6 212.5 7 450.0 8 828.5 9 1037.5 10 945.0 11 874.5 12 954.0 13 1034.5 14 1110.0 15 1196.5 16 1277.0 17 1346.5 18 1418.0 19 1542.0 20 1736.5 21 2054.5 22 2410.0 23 2828.5 24 3413.5 25 4194.0 26 5299.5 27 6557.5 28 7690.5 29 9039.0 30 10608.0 31 12272.5 32 14086.5 33 15809.0 34 17975.5 35 19871.5 36 21676.0 37 23956.0 38 25470.0 39 26086.5 40 26800.0 41 28310.0 42 29498.0 43 30473.0 44 31733.5 45 32602.5 46 32693.5 47 34486.0 48 40756.0 49 40478.0 50 33559.5 51 29929.5 52 27593.0 53 26152.0 54 24760.0 55 22226.0 56 19855.5 57 17548.5 58 15590.0 59 13957.0 60 11951.5 61 9591.5 62 7185.5 63 5408.0 64 4057.5 65 3043.0 66 2182.5 67 1626.5 68 1150.0 69 733.5 70 517.5 71 328.0 72 222.0 73 160.0 74 119.0 75 98.5 76 64.5 77 37.0 78 24.0 79 17.0 80 15.0 81 11.5 82 6.0 83 5.5 84 5.5 85 4.0 86 2.5 87 3.0 88 1.5 89 2.0 90 4.0 91 3.0 92 1.0 93 0.0 94 0.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026535431229981532 2 0.010754447899375774 3 0.01355995604703902 4 0.023613026909498985 5 0.057863605545554433 6 0.04453744184415402 7 0.01589787950342506 8 0.05190190073177004 9 0.03167886283403081 10-11 0.02396371542795689 12-13 0.036471605919622195 14-15 0.01958010894723307 16-17 0.020223037897739226 18-19 0.01917097234236551 20-21 0.016365464194702264 22-23 0.008182732097351132 24-25 0.002279475369976387 26-27 5.8448086409650946E-5 28-29 0.0014612021602412736 30-31 0.0 32-33 5.260327776868585E-4 34-35 0.0 36-37 0.0 38-39 1.1689617281930189E-4 40-41 0.0 42-43 0.0 44-45 5.8448086409650946E-5 46-47 0.0 48-49 5.260327776868585E-4 50-51 0.0 52-53 6.429289505061605E-4 54-55 0.001110513641783368 56-57 1.7534425922895286E-4 58-59 7.598251233254624E-4 60-61 0.0026886119748439435 62-63 0.003156196666121151 64-65 0.001052065555373717 66-67 0.011923409627568793 68-69 0.014904262034460991 70-71 0.0246650924648727 72-73 0.018878731910317255 74-75 0.019287868515184815 76-77 0.03267248030299488 78-79 0.02045683024337783 80-81 0.049388633016155044 82-83 0.037874359993453816 84-85 0.019638557033642718 86-87 0.014144436911135528 88-89 0.012215650059617047 90-91 0.024489748205643747 92-93 0.03138662240198256 94-95 0.01285857901012321 96-97 0.021041311107474342 98-99 0.03232179178453697 100-101 0.042608654992635546 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 855460.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.49550486779772 #Duplication Level Percentage of deduplicated Percentage of total 1 84.09333525955063 55.077354488412446 2 9.788565845662054 12.822141239866355 3 2.6044220201793746 5.117338053013736 4 1.0837921666969457 2.839340605183234 5 0.614464672218207 2.0122336965178658 6 0.36013188595412177 1.4152211813734417 7 0.24521592132216727 1.124237839803226 8 0.17984684992751293 0.9423328187908407 9 0.13635066093535536 0.8037319839317105 >10 0.80621315477505 9.670300801275337 >50 0.05975453265709713 2.717251385313283 >100 0.024865879442085896 2.9155514372289617 >500 0.001788912164333851 0.7581792794310885 >1k 0.001252238515033696 1.7847851898584102 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3826 0.4472447572066491 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2879 0.3365440815467702 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2486 0.2906038856287845 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1850 0.21625791971570849 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1812 0.21181586514857506 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1356 0.15851121034297339 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1024 0.11970168096696514 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 1.1689617281930189E-4 0.0 0.0 0.0 8 0.0 1.1689617281930189E-4 0.0 0.0 0.0 9 0.0 1.1689617281930189E-4 0.0 0.0 0.0 10-11 0.0 1.1689617281930189E-4 0.0 5.8448086409650946E-5 0.0 12-13 0.0 1.1689617281930189E-4 0.0 2.3379234563860378E-4 0.0 14-15 0.0 1.1689617281930189E-4 0.0 4.6758469127720757E-4 0.0 16-17 0.0 1.1689617281930189E-4 0.0 6.429289505061604E-4 0.0 18-19 0.0 1.1689617281930189E-4 0.0 9.351693825544152E-4 0.0 20-21 0.0 1.1689617281930189E-4 0.0 0.001052065555373717 0.0 22-23 0.0 1.1689617281930189E-4 0.0 0.001168961728193019 0.0 24-25 0.0 1.1689617281930189E-4 0.0 0.001168961728193019 0.0 26-27 0.0 1.1689617281930189E-4 0.0 0.0018118906786991794 0.0 28-29 0.0 1.1689617281930189E-4 0.0 0.0035653332709887076 0.0 30-31 0.0 1.1689617281930189E-4 0.0 0.007189114628387066 5.8448086409650946E-5 32-33 0.0 1.1689617281930189E-4 0.0 0.010345311294508218 1.1689617281930189E-4 34-35 0.0 1.1689617281930189E-4 0.0 0.012507890491665303 1.1689617281930189E-4 36-37 0.0 1.1689617281930189E-4 0.0 0.016833048885979474 1.1689617281930189E-4 38-39 0.0 1.1689617281930189E-4 0.0 0.023145442218221776 1.1689617281930189E-4 40-41 0.0 1.1689617281930189E-4 0.0 0.03138662240198256 1.1689617281930189E-4 42-43 5.8448086409650946E-5 1.1689617281930189E-4 0.0 0.03904332172164683 1.1689617281930189E-4 44-45 1.1689617281930189E-4 1.1689617281930189E-4 0.0 0.042082622214948684 1.1689617281930189E-4 46-47 2.9224043204825474E-4 1.1689617281930189E-4 0.0 0.04763519042386552 1.1689617281930189E-4 48-49 4.6758469127720757E-4 1.1689617281930189E-4 0.0 0.05377223949687887 1.1689617281930189E-4 50-51 4.6758469127720757E-4 1.1689617281930189E-4 0.0 0.0616042830757721 1.1689617281930189E-4 52-53 4.6758469127720757E-4 2.3379234563860378E-4 0.0 0.07656699319664274 1.1689617281930189E-4 54-55 4.6758469127720757E-4 2.9224043204825474E-4 0.0 0.08708764875037991 1.1689617281930189E-4 56-57 4.6758469127720757E-4 3.5068851845790566E-4 0.0 0.11812358263390457 1.1689617281930189E-4 58-59 4.6758469127720757E-4 3.5068851845790566E-4 0.0 0.17394150515512125 1.1689617281930189E-4 60-61 4.6758469127720757E-4 3.5068851845790566E-4 0.0 0.21614102354288922 1.1689617281930189E-4 62-63 4.6758469127720757E-4 3.5068851845790566E-4 0.0 0.2373576789095925 3.5068851845790566E-4 64-65 4.6758469127720757E-4 3.5068851845790566E-4 0.0 0.2785635798283964 3.5068851845790566E-4 66-67 4.6758469127720757E-4 3.5068851845790566E-4 0.0 0.37506137049073013 3.5068851845790566E-4 68-69 4.6758469127720757E-4 3.5068851845790566E-4 0.0 0.5189605592312907 3.5068851845790566E-4 70-71 4.6758469127720757E-4 3.5068851845790566E-4 0.0 0.63965585766722 3.5068851845790566E-4 72-73 4.6758469127720757E-4 3.5068851845790566E-4 0.0 0.7899843359128422 3.5068851845790566E-4 74-75 4.6758469127720757E-4 3.5068851845790566E-4 0.0 0.9662637645243495 3.5068851845790566E-4 76-77 4.6758469127720757E-4 3.5068851845790566E-4 0.0 1.0714703200617213 3.5068851845790566E-4 78-79 4.6758469127720757E-4 3.5068851845790566E-4 0.0 1.1832815093633835 3.5068851845790566E-4 80-81 4.6758469127720757E-4 3.5068851845790566E-4 0.0 1.309646272181049 3.5068851845790566E-4 82-83 4.6758469127720757E-4 3.5068851845790566E-4 0.0 1.449629439132163 3.5068851845790566E-4 84-85 4.6758469127720757E-4 3.5068851845790566E-4 0.0 1.6356696981740817 3.5068851845790566E-4 86-87 4.6758469127720757E-4 3.5068851845790566E-4 0.0 1.9637972552778622 3.5068851845790566E-4 88-89 4.6758469127720757E-4 3.5068851845790566E-4 0.0 2.4284010941481773 3.5068851845790566E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 3780 0.0 25.275408 1 TACATGG 3755 0.0 25.065397 2 ACATGGG 3780 0.0 24.270842 3 GAGTACT 3275 0.0 23.718348 12-13 GTGTAGC 475 0.0 23.01591 1 GTATCAA 7660 0.0 22.215096 1 CATGGGA 2465 0.0 22.17687 4 GTACTTT 3565 0.0 21.58652 14-15 AGTACTT 3435 0.0 21.36878 12-13 GTCCTAC 180 5.135342E-6 21.125715 1 ATCAACG 8545 0.0 19.748568 3 CAACGCA 8580 0.0 19.723412 5 TATCAAC 8590 0.0 19.70045 2 TCAACGC 8585 0.0 19.601183 4 AACGCAG 8715 0.0 19.469013 6 CATGGGG 1460 0.0 18.884022 4 AGAGTAC 6215 0.0 18.578327 10-11 GTTCGGT 90 1.2382974E-4 18.463392 36-37 ACTTTTT 4270 0.0 18.411306 16-17 GTAAGAC 155 9.573426E-4 18.400892 3 >>END_MODULE