FastQCFastQC Report
Thu 26 May 2016
SRR936913_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936913_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855460
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49800.5821429406401234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39040.4563626586865546No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34890.40785074696654433No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24850.2904869894559652No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13960.16318705725574545No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10500.12274098146026699No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9940.11619479578238608No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT9820.11479204170855446No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8610.10064760479741894No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC8560.10006312393332244No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC550.002576130434.5541
GTTACGT950.001227455424.9959563
AAGGGTA2551.8189894E-1124.2117674
GTACATG38050.023.9743921
TTACGTA1000.001654859323.7461574
TACATGG38800.022.8893362
GAGTACT46300.022.56654212-13
ACATGGG37000.022.3342233
AGTACTT48400.022.17616312-13
GAGTACG1503.0262569E-522.172151
GTACTTT49700.021.11831314-15
CGTACAC1353.7989987E-421.1076953
GTATCAA94600.020.943341
TCACGCA1150.00372600220.6488324
CATGGGG14800.020.5372184
AGAGTAC74700.019.33091710-11
TACTTTT54650.019.29239514-15
ACTTTTT56100.018.66676516-17
TCAACGC106850.018.4013274
TATCAAC107800.018.3272742
Produced by FastQC (version 0.11.5)