##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936913_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 855460 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.42434362798962 33.0 31.0 34.0 31.0 34.0 2 32.5645699389802 34.0 31.0 34.0 31.0 34.0 3 32.66378907254577 34.0 31.0 34.0 31.0 34.0 4 36.247401398078225 37.0 35.0 37.0 35.0 37.0 5 36.183609987609 37.0 35.0 37.0 35.0 37.0 6 36.237919949500856 37.0 37.0 37.0 35.0 37.0 7 36.20304163841676 37.0 36.0 37.0 35.0 37.0 8 36.17491291235125 37.0 36.0 37.0 35.0 37.0 9 37.897893530965796 39.0 38.0 39.0 35.0 39.0 10-11 37.820145301942816 39.0 38.0 39.0 35.0 39.0 12-13 37.73893869964697 39.0 37.0 39.0 35.0 39.0 14-15 38.99921738012297 40.0 38.0 41.0 36.0 41.0 16-17 38.92739695602366 40.0 38.0 41.0 36.0 41.0 18-19 39.08771421223669 40.0 38.5 41.0 36.0 41.0 20-21 39.12002782128913 40.0 39.0 41.0 36.0 41.0 22-23 39.04887604329834 40.0 38.0 41.0 35.5 41.0 24-25 38.94253851728894 40.0 38.0 41.0 35.0 41.0 26-27 38.770559114394594 40.0 38.0 41.0 35.0 41.0 28-29 38.689212821172234 40.0 38.0 41.0 35.0 41.0 30-31 38.68078869847801 40.0 38.0 41.0 35.0 41.0 32-33 38.51274343627989 40.0 38.0 41.0 35.0 41.0 34-35 38.35379211184626 40.0 38.0 41.0 34.0 41.0 36-37 38.193457321207305 40.0 38.0 41.0 34.0 41.0 38-39 38.03503261403222 40.0 38.0 41.0 34.0 41.0 40-41 37.86559278049236 40.0 37.5 41.0 33.0 41.0 42-43 37.61049669183831 40.0 37.0 41.0 33.0 41.0 44-45 37.30954807939588 40.0 37.0 41.0 33.0 41.0 46-47 36.88846234774273 40.0 37.0 41.0 31.5 41.0 48-49 36.46910258808127 40.0 36.0 41.0 30.5 41.0 50-51 36.16744500035069 39.0 36.0 41.0 30.0 41.0 52-53 35.93292497603629 39.0 35.5 40.5 29.5 41.0 54-55 35.64875739368293 39.0 35.0 40.0 28.5 41.0 56-57 35.36540457765413 38.5 35.0 40.0 28.0 41.0 58-59 35.03568489467655 38.0 34.5 40.0 27.0 41.0 60-61 34.69567893297173 38.0 34.0 40.0 26.0 41.0 62-63 34.348228438500925 37.5 34.0 40.0 26.0 41.0 64-65 33.97392513969093 37.0 34.0 39.5 26.0 41.0 66-67 33.57707549154841 36.0 33.0 39.0 25.0 40.0 68-69 33.28279112991841 36.0 33.0 39.0 24.5 40.0 70-71 32.80350922310804 35.5 32.5 38.5 22.5 40.0 72-73 32.29440418020714 35.0 32.0 38.0 21.5 40.0 74-75 31.805733757276787 35.0 31.0 37.0 20.0 39.0 76-77 30.415456011970168 33.5 29.5 35.5 19.5 38.0 78-79 31.1185257054684 34.0 31.0 36.0 19.5 38.5 80-81 30.835691323966053 34.0 31.0 36.0 17.5 37.5 82-83 30.527536062469316 34.0 31.0 35.0 15.0 37.0 84-85 30.198225516096606 34.0 30.5 35.0 12.0 36.5 86-87 29.881824983050056 34.0 30.0 35.0 7.5 36.0 88-89 29.50131975779113 34.0 30.0 35.0 4.5 36.0 90-91 29.05872629930096 34.0 29.0 35.0 2.0 35.0 92-93 28.623949103406353 34.0 29.0 35.0 2.0 35.0 94-95 28.061023893577726 33.5 29.0 35.0 2.0 35.0 96-97 27.414417973955533 33.0 27.0 35.0 2.0 35.0 98-99 26.65908575503238 33.0 25.5 34.0 2.0 35.0 100-101 23.164435508381455 29.0 12.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.013478721194296384 1101 2 -0.020882164053631413 1101 3 0.005497556411810933 1101 4 0.09118904266627226 1101 5 0.06704074085961764 1101 6 0.10423051091691349 1101 7 0.14765414230058838 1101 8 0.16388594300111947 1101 9 0.061448342668604994 1101 10-11 0.04213381326623988 1101 12-13 0.04026708396798284 1101 14-15 0.12244522199141983 1101 16-17 0.1167400076555083 1101 18-19 -0.022161791772887796 1101 20-21 0.08608299087199356 1101 22-23 0.10755412262613362 1101 24-25 0.04757447346862875 1101 26-27 -0.13150549944885626 1101 28-29 -5.3675499086836E-4 1101 30-31 0.04599957806847499 1101 32-33 -0.011996596461976594 1101 34-35 0.16475668214851424 1101 36-37 0.03741552455817754 1101 38-39 0.022107806359642268 1101 40-41 0.23004171295442433 1101 42-43 0.06584909094882363 1101 44-45 0.09898405398874388 1101 46-47 0.008478977162738488 1101 48-49 0.1806359676950109 1101 50-51 0.019480563335903867 1101 52-53 0.09520937350882974 1101 54-55 0.02190724885405615 1101 56-57 0.012744996754008753 1101 58-59 -0.07910223144042305 1101 60-61 0.15010095373071408 1101 62-63 -0.07577634776066589 1101 64-65 -0.06181639094858582 1101 66-67 -0.3590985903266315 1101 68-69 -0.3367972747415635 1101 70-71 -0.35178045449334405 1101 72-73 -0.2714104792410623 1101 74-75 -0.21847612329574062 1101 76-77 -0.26325168519828424 1101 78-79 -0.10502048267821351 1101 80-81 0.04187015189249266 1101 82-83 -0.13076649975624477 1101 84-85 -0.17627274059452347 1101 86-87 -0.6131165263171354 1101 88-89 -0.6175194232642269 1101 90-91 -0.2517221151106277 1101 92-93 -0.4779085985715419 1101 94-95 -0.7576595804439954 1101 96-97 -0.3193424021539393 1101 98-99 0.039879837417934993 1101 100-101 0.05238139668341546 1102 1 0.11346862884933984 1102 2 0.19868656725721934 1102 3 0.07448198978362086 1102 4 0.09938500344937751 1102 5 0.06646612831613652 1102 6 0.04035898972380636 1102 7 -0.013492934093576991 1102 8 0.05894671682353447 1102 9 0.14557943246280303 1102 10-11 0.05395040280243535 1102 12-13 0.040946282505565534 1102 14-15 0.14958223534098636 1102 16-17 0.17491057479647054 1102 18-19 0.05717258552128612 1102 20-21 0.12695454624886082 1102 22-23 0.03453559991755384 1102 24-25 -0.012619039106922969 1102 26-27 0.05236685192102186 1102 28-29 0.16060055461785794 1102 30-31 0.0699827336191845 1102 32-33 0.0923590642854677 1102 34-35 0.03720449902780132 1102 36-37 -0.035103264295322845 1102 38-39 0.15592660318934293 1102 40-41 -0.03988212239710265 1102 42-43 -0.11274594068742516 1102 44-45 -0.28358988464654544 1102 46-47 -0.019878070468244857 1102 48-49 -0.0322701881470735 1102 50-51 -0.09463659251106549 1102 52-53 5.993368345613703E-4 1102 54-55 0.02357207882246115 1102 56-57 0.029225226338574828 1102 58-59 0.19919906412366117 1102 60-61 0.042249190744549026 1102 62-63 -0.11964630626810191 1102 64-65 0.052973452245673514 1102 66-67 0.0570714624302866 1102 68-69 0.036362296626776924 1102 70-71 -0.133531867014689 1102 72-73 -0.025890178163628264 1102 74-75 -0.10689873861299048 1102 76-77 -0.12338887597778836 1102 78-79 -0.22731976302890544 1102 80-81 0.08819808473607438 1102 82-83 -0.02349179100992771 1102 84-85 -0.12174314967633393 1102 86-87 -0.1409168671779497 1102 88-89 -0.27907589074815675 1102 90-91 0.09087392901570723 1102 92-93 0.10869633658514033 1102 94-95 -0.12208398308298385 1102 96-97 -0.18402521765773372 1102 98-99 -0.0945656614776027 1102 100-101 -0.054745182483706856 1103 1 0.16510551545267305 1103 2 0.132880762323893 1103 3 0.11246159407194511 1103 4 0.07315385205043867 1103 5 0.015352208678727663 1103 6 0.04806752280146753 1103 7 0.016704921741862222 1103 8 0.02766893420346861 1103 9 0.11438706778050545 1103 10-11 0.06275890973174825 1103 12-13 0.13687149822943212 1103 14-15 0.2086452528603786 1103 16-17 0.20773939027628785 1103 18-19 0.07975517072132021 1103 20-21 0.057534603775224014 1103 22-23 0.09369318729661558 1103 24-25 0.11513351035703323 1103 26-27 0.13445915559051258 1103 28-29 0.06346772100851439 1103 30-31 0.13148190558815998 1103 32-33 0.2821128340056802 1103 34-35 0.2822524376663367 1103 36-37 0.24760614039394824 1103 38-39 0.20604514236828209 1103 40-41 0.2155711156400244 1103 42-43 0.19205838250615415 1103 44-45 0.2865569819097189 1103 46-47 0.44025702933128485 1103 48-49 0.6026038639752969 1103 50-51 0.801225431371094 1103 52-53 0.7579945255384715 1103 54-55 0.6915869833809936 1103 56-57 0.6978947112161151 1103 58-59 0.6848366480162795 1103 60-61 0.5157630929497259 1103 62-63 0.7407354596170208 1103 64-65 0.4617466589047794 1103 66-67 0.39474382362613625 1103 68-69 0.3992399223567489 1103 70-71 0.2950269231883951 1103 72-73 0.47523637984212996 1103 74-75 0.5331553013068238 1103 76-77 0.46688568167931876 1103 78-79 0.7052419034924924 1103 80-81 0.6797857252834483 1103 82-83 0.7913966427040116 1103 84-85 0.539742076506883 1103 86-87 0.36133014755228743 1103 88-89 0.31410505582205417 1103 90-91 0.5026520166998374 1103 92-93 0.5703449899452018 1103 94-95 0.18381595590420474 1103 96-97 0.35204207075854654 1103 98-99 0.3657001800634063 1103 100-101 0.6688044557974742 1104 1 0.14728699879437812 1104 2 0.14116638094100864 1104 3 0.18374235750740553 1104 4 0.036564198609958964 1104 5 0.07753704042157494 1104 6 -0.07171148272339423 1104 7 0.05521823163638828 1104 8 0.06782463485640733 1104 9 0.12809374784077932 1104 10-11 0.18337501554122326 1104 12-13 0.10409065108395055 1104 14-15 0.0727621120601114 1104 16-17 0.12375395582124327 1104 18-19 0.1511866122080079 1104 20-21 0.1526635172190538 1104 22-23 0.03330226146369597 1104 24-25 0.030007945314338258 1104 26-27 -0.0035527312335332795 1104 28-29 -0.01397996022369341 1104 30-31 0.04114539127374428 1104 32-33 -0.03318852209677203 1104 34-35 0.131543413725268 1104 36-37 0.16087836540651068 1104 38-39 0.15598487114779402 1104 40-41 0.1099181774180309 1104 42-43 0.08406909738980772 1104 44-45 -0.11391932815222816 1104 46-47 -0.011673323925037948 1104 48-49 -0.19174738330412566 1104 50-51 -0.0517337181350257 1104 52-53 -0.009056370158837979 1104 54-55 -0.05634286761515028 1104 56-57 -0.1906696617957735 1104 58-59 0.0052418045540321145 1104 60-61 0.21028427158692864 1104 62-63 0.16168640554034397 1104 64-65 0.05389382113481389 1104 66-67 0.0144299110194126 1104 68-69 0.1514088491941905 1104 70-71 -0.10956122347158015 1104 72-73 0.12086253085501397 1104 74-75 0.2487772668182906 1104 76-77 -0.015153494614001062 1104 78-79 -0.04543279933690414 1104 80-81 0.11302111788348057 1104 82-83 0.3381964083159339 1104 84-85 0.2838455421020605 1104 86-87 0.15414280450858797 1104 88-89 0.08782596658716457 1104 90-91 0.1681752043777216 1104 92-93 -0.040402540608955206 1104 94-95 -0.12504726190929105 1104 96-97 -0.0704508138890354 1104 98-99 -0.054880767534115193 1104 100-101 0.4519896223804345 1105 1 0.07290854575570194 1105 2 0.10369515626329218 1105 3 -0.06763310289775148 1105 4 0.006108397504981156 1105 5 -0.041598548897027854 1105 6 0.051400856134797834 1105 7 0.06801173992367637 1105 8 -0.009736368826835928 1105 9 0.22881888596232614 1105 10-11 0.07879110670143774 1105 12-13 -0.07719100177056504 1105 14-15 0.02719638922401657 1105 16-17 -0.028283336996437924 1105 18-19 0.011781685872833236 1105 20-21 0.09878460377522202 1105 22-23 0.002187505478431717 1105 24-25 -0.13795361085509228 1105 26-27 -0.02940448077313107 1105 28-29 -0.016825839597544245 1105 30-31 0.005383420739676126 1105 32-33 -0.06806709023673818 1105 34-35 -0.14556006233366503 1105 36-37 -0.24287681415150075 1105 38-39 -0.26911584248020404 1105 40-41 -0.08535691466300221 1105 42-43 -0.050924572039299676 1105 44-45 -0.19226877566603662 1105 46-47 -0.005538425214169251 1105 48-49 -0.053807120873180736 1105 50-51 -0.06818744741678984 1105 52-53 -0.0588520653706226 1105 54-55 0.08375554398705987 1105 56-57 0.17621857485247716 1105 58-59 0.06619081468294752 1105 60-61 -0.07173690705027269 1105 62-63 -0.03267363129207723 1105 64-65 -0.3682344017012795 1105 66-67 -0.30234897940416516 1105 68-69 -0.05851575946142873 1105 70-71 -0.22666815256918227 1105 72-73 -0.0591386201578743 1105 74-75 -0.01199621384469296 1105 76-77 -0.21430750013886168 1105 78-79 0.006984327734915752 1105 80-81 0.16565693740465903 1105 82-83 0.0369648245221903 1105 84-85 -0.10300413561433075 1105 86-87 -0.3272683372961964 1105 88-89 -0.34046880781430744 1105 90-91 -0.24517942269410398 1105 92-93 -0.10436144944873504 1105 94-95 -0.12113669561094653 1105 96-97 0.026757979849456603 1105 98-99 -0.0759759563002298 1105 100-101 0.4119199861005036 1106 1 0.20557502324340504 1106 2 0.11217392092466127 1106 3 0.027168022717077633 1106 4 0.05010669489431763 1106 5 0.1443182596538577 1106 6 0.07680759090589362 1106 7 0.06914326659791215 1106 8 0.09896704585344906 1106 9 0.2792090675741292 1106 10-11 -0.010403818572868317 1106 12-13 0.21876707144169671 1106 14-15 0.1922166077257188 1106 16-17 0.11716927367325525 1106 18-19 -0.02415877004847289 1106 20-21 0.11557660150507587 1106 22-23 -0.057313416769012804 1106 24-25 0.06366978112372124 1106 26-27 0.2240149289885025 1106 28-29 0.09134255332716634 1106 30-31 0.16828306129963977 1106 32-33 0.1533320064337076 1106 34-35 0.14582224470380112 1106 36-37 0.30222640662034195 1106 38-39 0.37030373281302786 1106 40-41 0.507944967667683 1106 42-43 0.4566502718880585 1106 44-45 0.3016467559268463 1106 46-47 0.2534641287018289 1106 48-49 0.2914935554416118 1106 50-51 0.2009184455079236 1106 52-53 0.28261891930588234 1106 54-55 0.09582338829276438 1106 56-57 0.13466955385361246 1106 58-59 0.16515446867462202 1106 60-61 0.16545094766028257 1106 62-63 0.24571740162506472 1106 64-65 -0.293829133067149 1106 66-67 0.1339136719390055 1106 68-69 0.02773388582801317 1106 70-71 -0.09582902563010265 1106 72-73 -0.0743947211794378 1106 74-75 -0.07088762511976299 1106 76-77 -0.2908008315804054 1106 78-79 0.0019852736297316653 1106 80-81 0.15810633203198066 1106 82-83 0.4671365044313873 1106 84-85 0.053492979406478014 1106 86-87 -0.16304917346721481 1106 88-89 -0.012420070583889498 1106 90-91 -0.06917854774139798 1106 92-93 0.07228854582178812 1106 94-95 0.34664435338640587 1106 96-97 0.6145949548778304 1106 98-99 0.4426055561855833 1106 100-101 0.7729118750524542 1107 1 0.06256835294887964 1107 2 0.07969742730518448 1107 3 0.13078296124759703 1107 4 0.10564955434591639 1107 5 0.10808049263059161 1107 6 -0.011687760847031825 1107 7 0.04959196795482512 1107 8 0.12495379617131164 1107 9 0.06472683924374678 1107 10-11 0.08737413877411626 1107 12-13 0.15005281079896093 1107 14-15 0.23356283805605216 1107 16-17 0.05428757512814286 1107 18-19 0.24469904775641282 1107 20-21 0.07329099977077647 1107 22-23 -0.028664482841925576 1107 24-25 0.13064923489945102 1107 26-27 0.13298428452164757 1107 28-29 0.13098364779585836 1107 30-31 6.860718334777971E-4 1107 32-33 0.1046242102823598 1107 34-35 -0.09973310182198247 1107 36-37 0.07829427872948003 1107 38-39 0.12186777357467093 1107 40-41 0.147991481703329 1107 42-43 0.010304919061930207 1107 44-45 -0.020241986641195808 1107 46-47 -0.04508931509180769 1107 48-49 0.15780358083241453 1107 50-51 0.039224723513875404 1107 52-53 -0.0025308056375834553 1107 54-55 -0.06668533940189292 1107 56-57 -0.024269815025157015 1107 58-59 0.08599348895065617 1107 60-61 -0.03582200159976878 1107 62-63 0.004019917095021697 1107 64-65 -0.09129358783402353 1107 66-67 -0.0678100240834354 1107 68-69 -0.029057681040960404 1107 70-71 0.11578584724542651 1107 72-73 -0.3187291373992487 1107 74-75 -0.33600437105641134 1107 76-77 -0.054042622497036064 1107 78-79 0.10967713455731243 1107 80-81 0.1401957768559008 1107 82-83 0.17338723831529634 1107 84-85 0.19744659668081255 1107 86-87 0.1264186297041725 1107 88-89 0.12055211821461143 1107 90-91 0.23238303095810053 1107 92-93 -0.020719323938909184 1107 94-95 0.18746951035864967 1107 96-97 0.37470361388727724 1107 98-99 0.1696146304628421 1107 100-101 0.3973407081991347 1108 1 0.07550192781010878 1108 2 0.0955128075016134 1108 3 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-1.2509811793723848 2316 92-93 -1.1227829796461783 2316 94-95 -1.4312648169814466 2316 96-97 -0.8449645811261561 2316 98-99 -1.6756111914918677 2316 100-101 -2.2590748600086705 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 3.0 5 9.0 6 26.0 7 60.0 8 146.0 9 353.0 10 539.0 11 1087.0 12 1748.0 13 2581.0 14 3151.0 15 4829.0 16 6704.0 17 10148.0 18 9267.0 19 4854.0 20 3883.0 21 3907.0 22 3856.0 23 4232.0 24 4749.0 25 5595.0 26 6888.0 27 8608.0 28 10445.0 29 13419.0 30 17223.0 31 21663.0 32 28731.0 33 39012.0 34 55587.0 35 83130.0 36 133439.0 37 206198.0 38 149087.0 39 10299.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.68381788928177 19.76857308562983 15.178270752651962 21.369338272436437 2 15.337332946776773 21.591015365623274 39.94091763539611 23.13073405220384 3 19.86404895640332 25.884587734012893 30.302821305620988 23.948542003962803 4 12.885378358727136 17.66433886876971 38.155584493920216 31.294698278582935 5 13.36754494657845 38.076941060949665 33.99726462955603 14.55824936291586 6 30.481495335842705 37.338040352558856 17.571365113506186 14.609099198092254 7 27.43202487550558 34.03934725177098 21.056156921422392 17.472470951301055 8 26.267037617188414 34.13321487854488 20.013910644565495 19.585836859701214 9 26.46050078320436 17.477029902041007 20.908984639842892 35.153484674911745 10-11 24.541425851545846 27.27302486784518 27.359890757083576 20.825658523525398 12-13 26.3481051130386 24.762525424917587 27.99061323732261 20.8987562247212 14-15 22.91398779603956 25.74842774647558 27.36516026465294 23.972424192831927 16-17 21.34150047927431 29.30429242746592 27.64939330886307 21.7048137843967 18-19 21.10714246052829 27.55710560877288 30.687663692224426 20.6480882384744 20-21 22.238133201785253 26.318013592782314 30.687330937724294 20.756522267708142 22-23 21.766672881802723 26.505645934804196 30.578352505761664 21.149328677631413 24-25 22.013926397725385 26.476172438545913 30.74697727490606 20.76292388882264 26-27 21.856618710797054 26.536276112646124 30.91332813890972 20.693777037647102 28-29 21.15414401550517 27.058184723594948 31.02127343704794 20.766397823851943 30-31 21.389593537752944 26.600481629122196 30.78936908922998 21.220555743894884 32-33 21.36170829604682 26.887145636878955 30.630721202489287 21.120424864584937 34-35 22.00389264255488 26.595165174292195 30.49733476725972 20.903607415893205 36-37 22.03758585415753 26.304661698085635 30.655677334502414 21.00207511325442 38-39 21.62614324679166 26.702842933118525 30.780535196744253 20.890478623345558 40-41 21.689189860559015 26.910818439151722 30.477823698947283 20.92216800134198 42-43 21.605159797068243 27.070874149580344 29.902157903350247 21.42180815000117 44-45 21.940418020714002 26.83860145418839 29.70220699974283 21.518773525354778 46-47 22.359147006134144 27.297031120774555 29.233129641156925 21.110692231934376 48-49 22.4262913695935 27.581248706789225 28.676897790465368 21.315562133151904 50-51 22.01370943182489 27.688738497293357 28.879355947797308 21.41819612308444 52-53 22.457508241180182 28.030182591821944 28.194187922287423 21.318121244710447 54-55 21.852556934916244 27.733936851531134 28.53306851478528 21.880437698767345 56-57 22.30461151428999 27.951577229537634 28.338344493490947 21.40546676268143 58-59 22.777349482938703 27.5277789305046 28.137334379944345 21.557537206612352 60-61 22.22287815458819 27.573279497522034 29.025572205197204 21.178270142692565 62-63 22.136694684420334 27.622660478600903 28.9579266082931 21.28271822868566 64-65 21.99705421644495 28.041638416758236 28.516003086058962 21.445304280737847 66-67 21.932318921816123 27.97828248319429 28.321322923857178 21.768075671132408 68-69 21.880201904013767 27.93077610069951 28.45242771106872 21.736594284218008 70-71 22.184958036877518 27.972473080068056 28.290082394893467 21.55248648816096 72-73 22.13697893530966 27.603277768685853 28.56153414537208 21.698209150632408 74-75 21.891555420475534 28.386891263180043 28.04742477731279 21.67412853903163 76-77 22.217929534987025 27.884179271970638 28.10756785822832 21.79032333481402 78-79 22.188756663237633 27.929369681099786 27.991969045169736 21.889904610492845 80-81 22.278710668425614 28.22071330918579 27.86983939759339 21.630736624795208 82-83 22.165208790746288 28.11128491829114 28.063702279991677 21.659804010970895 84-85 22.06032612253678 28.16173788614236 27.891976001421583 21.885959989899273 86-87 22.088688390057772 28.205260093848416 27.747591247360955 21.95846026873286 88-89 22.067951745259858 28.105989759895262 27.49672690716106 22.32933158768382 90-91 22.0144379391306 27.84648394508416 27.88517682705509 22.253901288730145 92-93 22.334325783000704 27.893329779685526 27.736980965773927 22.03536347153984 94-95 21.993443289834293 27.88705952765736 27.678925770302392 22.440571412205955 96-97 21.741321012224667 27.96552661341937 27.980781876046606 22.312370498309356 98-99 21.805816061063204 28.37201608759921 27.27883788663515 22.543329964702437 100-101 21.350309074926123 28.81960030673699 27.185793307896606 22.64429731044028 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 241.0 1 192.0 2 154.0 3 193.5 4 184.0 5 145.0 6 245.5 7 558.0 8 966.0 9 1200.0 10 1174.5 11 1141.5 12 1204.0 13 1310.5 14 1461.5 15 1577.0 16 1664.5 17 1774.0 18 1879.0 19 1984.5 20 2166.0 21 2423.0 22 2800.5 23 3168.5 24 3591.0 25 4405.0 26 5477.5 27 6685.0 28 7785.5 29 9017.5 30 10762.0 31 12378.0 32 14057.5 33 15782.0 34 17902.0 35 19690.5 36 21548.5 37 23606.0 38 25044.5 39 25902.0 40 26492.5 41 28071.5 42 29424.5 43 30226.5 44 31429.0 45 32171.0 46 31997.5 47 34554.5 48 41226.5 49 40061.5 50 32756.0 51 29205.0 52 26917.5 53 25613.5 54 24191.5 55 21988.5 56 19729.5 57 17433.0 58 15611.0 59 14103.0 60 12068.0 61 9519.0 62 7215.5 63 5529.0 64 4064.5 65 2994.0 66 2240.0 67 1752.0 68 1197.5 69 698.5 70 492.5 71 319.0 72 234.5 73 185.5 74 139.5 75 102.0 76 73.0 77 45.0 78 23.0 79 11.0 80 9.0 81 7.0 82 5.0 83 5.0 84 3.0 85 0.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028522666167909664 2 0.005143431604049284 3 5.844808640965095E-4 4 2.3379234563860378E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.013852196479087274 12-13 0.0 14-15 0.0 16-17 0.0 18-19 6.429289505061605E-4 20-21 7.013770369158114E-4 22-23 0.002513267715614991 24-25 0.012390994318846 26-27 0.006487737591471255 28-29 4.0913660486755664E-4 30-31 0.0035068851845790573 32-33 0.004032917962265915 34-35 0.0 36-37 0.009936174689640661 38-39 0.01297547518294251 40-41 9.936174689640662E-4 42-43 0.0 44-45 0.0 46-47 2.9224043204825474E-4 48-49 0.0036237813573983587 50-51 0.00742290697402567 52-53 0.0 54-55 0.00368222944380801 56-57 0.004208262221494868 58-59 5.8448086409650946E-5 60-61 0.002279475369976387 62-63 0.005844808640965094 64-65 0.0 66-67 0.002513267715614991 68-69 4.6758469127720757E-4 70-71 7.598251233254624E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.002337923456386038 80-81 0.0028639562340728964 82-83 0.012390994318846 84-85 0.008884109134266944 86-87 0.004442054567133472 88-89 0.0 90-91 6.429289505061605E-4 92-93 1.1689617281930189E-4 94-95 5.8448086409650946E-5 96-97 0.002045683024337783 98-99 0.002162579197157085 100-101 4.6758469127720757E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 855460.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.29286933367515 #Duplication Level Percentage of deduplicated Percentage of total 1 82.5200555204145 51.404110359407994 2 10.567362102535013 13.165426133096886 3 2.975267639195178 5.56013874843292 4 1.23798949711908 3.0847167192200415 5 0.6573124204809718 2.047293836021146 6 0.4123507457585304 1.5411906675107783 7 0.27726105155918457 1.2089970519265558 8 0.20249241209280536 1.009106669404626 9 0.173121555088444 0.970581456897038 >10 0.8727257653929158 10.035860682283479 >50 0.06466290062444846 2.7542975417821003 >100 0.03581671797382823 4.0221332638957294 >500 0.0024506175234767997 1.1641781200079597 >1k 0.0011310542416046766 2.0319687501127435 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4980 0.5821429406401234 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3904 0.4563626586865546 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3489 0.40785074696654433 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2485 0.2904869894559652 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1396 0.16318705725574545 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1050 0.12274098146026699 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 994 0.11619479578238608 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 982 0.11479204170855446 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 861 0.10064760479741894 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 856 0.10006312393332244 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 1.1689617281930189E-4 0.0 0.0 0.0 12-13 1.1689617281930189E-4 1.1689617281930189E-4 0.0 5.8448086409650946E-5 0.0 14-15 1.1689617281930189E-4 1.1689617281930189E-4 0.0 1.1689617281930189E-4 0.0 16-17 1.1689617281930189E-4 1.1689617281930189E-4 0.0 1.1689617281930189E-4 0.0 18-19 1.1689617281930189E-4 1.1689617281930189E-4 0.0 1.7534425922895283E-4 0.0 20-21 1.1689617281930189E-4 1.1689617281930189E-4 0.0 2.3379234563860378E-4 0.0 22-23 1.1689617281930189E-4 1.1689617281930189E-4 0.0 3.5068851845790566E-4 0.0 24-25 2.3379234563860378E-4 1.1689617281930189E-4 0.0 3.5068851845790566E-4 0.0 26-27 2.3379234563860378E-4 1.1689617281930189E-4 0.0 9.936174689640662E-4 0.0 28-29 2.3379234563860378E-4 2.3379234563860378E-4 0.0 0.0027470600612535946 0.0 30-31 2.3379234563860378E-4 3.5068851845790566E-4 0.0 0.006487737591471255 0.0 32-33 2.3379234563860378E-4 3.5068851845790566E-4 0.0 0.00987772660323101 0.0 34-35 2.3379234563860378E-4 3.5068851845790566E-4 0.0 0.012390994318846 0.0 36-37 2.3379234563860378E-4 3.5068851845790566E-4 0.0 0.016833048885979474 0.0 38-39 2.3379234563860378E-4 3.5068851845790566E-4 0.0 0.023262338391041075 0.0 40-41 2.3379234563860378E-4 3.5068851845790566E-4 0.0 0.031737310920440465 0.0 42-43 2.9224043204825474E-4 3.5068851845790566E-4 0.0 0.03968625067215299 0.0 44-45 3.5068851845790566E-4 3.5068851845790566E-4 0.0 0.04284244733827415 0.0 46-47 3.5068851845790566E-4 3.5068851845790566E-4 0.0 0.048219671287962035 0.0 48-49 3.5068851845790566E-4 3.5068851845790566E-4 0.0 0.054473616533794685 0.0 50-51 3.5068851845790566E-4 3.5068851845790566E-4 0.0 0.06218876393986861 0.0 52-53 3.5068851845790566E-4 3.5068851845790566E-4 0.0 0.0769761298015103 0.0 54-55 3.5068851845790566E-4 3.5068851845790566E-4 0.0 0.08714609683678956 0.0 56-57 4.6758469127720757E-4 3.5068851845790566E-4 0.0 0.11771444602903701 0.0 58-59 4.6758469127720757E-4 3.5068851845790566E-4 0.0 0.17294788768615715 0.0 60-61 4.6758469127720757E-4 3.5068851845790566E-4 0.0 0.2153227503331541 0.0 62-63 4.6758469127720757E-4 3.5068851845790566E-4 0.0 0.23683164613190563 0.0 64-65 4.6758469127720757E-4 3.5068851845790566E-4 0.0 0.27850513174198677 0.0 66-67 4.6758469127720757E-4 3.5068851845790566E-4 0.0 0.3747106819722722 0.0 68-69 4.6758469127720757E-4 3.5068851845790566E-4 0.0 0.5183760783671942 0.0 70-71 4.6758469127720757E-4 3.5068851845790566E-4 0.0 0.6385453440254366 5.8448086409650946E-5 72-73 4.6758469127720757E-4 3.5068851845790566E-4 0.0 0.7864774507282631 1.1689617281930189E-4 74-75 4.6758469127720757E-4 3.5068851845790566E-4 0.0 0.9618217099572159 1.1689617281930189E-4 76-77 4.6758469127720757E-4 3.5068851845790566E-4 0.0 1.0662684403712621 1.1689617281930189E-4 78-79 4.6758469127720757E-4 3.5068851845790566E-4 0.0 1.1774367007224185 1.1689617281930189E-4 80-81 4.6758469127720757E-4 3.5068851845790566E-4 0.0 1.3050288733546864 1.1689617281930189E-4 82-83 4.6758469127720757E-4 3.5068851845790566E-4 0.0 1.4461225539475837 1.1689617281930189E-4 84-85 4.6758469127720757E-4 3.5068851845790566E-4 0.0 1.631286091693358 1.1689617281930189E-4 86-87 4.6758469127720757E-4 3.5068851845790566E-4 0.0 1.9588876160194515 1.1689617281930189E-4 88-89 4.6758469127720757E-4 3.5068851845790566E-4 0.0 2.4253617936548757 1.1689617281930189E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTAC 55 0.0025761304 34.554 1 GTTACGT 95 0.0012274554 24.995956 3 AAGGGTA 255 1.8189894E-11 24.211767 4 GTACATG 3805 0.0 23.974392 1 TTACGTA 100 0.0016548593 23.746157 4 TACATGG 3880 0.0 22.889336 2 GAGTACT 4630 0.0 22.566542 12-13 ACATGGG 3700 0.0 22.334223 3 AGTACTT 4840 0.0 22.176163 12-13 GAGTACG 150 3.0262569E-5 22.17215 1 GTACTTT 4970 0.0 21.118313 14-15 CGTACAC 135 3.7989987E-4 21.107695 3 GTATCAA 9460 0.0 20.94334 1 TCACGCA 115 0.003726002 20.648832 4 CATGGGG 1480 0.0 20.537218 4 AGAGTAC 7470 0.0 19.330917 10-11 TACTTTT 5465 0.0 19.292395 14-15 ACTTTTT 5610 0.0 18.666765 16-17 TCAACGC 10685 0.0 18.401327 4 TATCAAC 10780 0.0 18.327274 2 >>END_MODULE