FastQCFastQC Report
Thu 26 May 2016
SRR936912_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936912_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873269
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38490.4407576588657103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29480.33758211959888645No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25250.28914343690203137No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19110.2188329140276364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19090.21860388952315954No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14390.16478313097109826No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC11200.12825372250703965No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT9020.10329005151906229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG1503.3072865E-828.5242395
GTACATG37650.027.1416781
TTAAGCG350.00840487127.1348250-51
TCGCGCT806.265327E-826.71007360-61
TACATGG38050.026.3627432
ACATGGG39150.023.6791253
ATTCGCG953.2792923E-722.50687480-81
GAGTACT34500.022.38387312-13
CCTCGCG1105.671609E-821.5808158-59
GTATCAA77200.020.9942461
CATGGGG14750.020.9500064
AATTCGC1158.995448E-820.65907778-79
GTACTTT37800.020.49317414-15
CGCGGAA1058.6042746E-720.35927882-83
TCGCGGA1058.6042746E-720.35927882-83
AGTACTT36400.020.04047212-13
GTACACG1456.1187753E-419.6673771
CTCGCGC1101.3497593E-619.4255160-61
CCTATAC2451.739154E-719.4042433
ATGGGGA8950.019.1223955
Produced by FastQC (version 0.11.5)