FastQCFastQC Report
Thu 26 May 2016
SRR936912_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936912_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873269
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51760.5927154175861047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40660.4656068176014493No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36400.4168245981478788No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26150.29944953960348986No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13630.1560801998009777No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11190.1281392102548012No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT10200.11680249728319682No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10200.11680249728319682No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT9200.10535127205935399No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8850.101343343231009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT46900.022.43546512-13
GGACGGT1100.00287621521.591696
CTTACAC4200.021.4864123
AGTACTT48550.021.42836812-13
GTACTTT50150.021.02647414-15
GTACATG36000.020.454471
GTATCAA102300.019.690161
TACATGG36700.019.5420672
TAAGGTG2702.2988388E-819.3503365
AGAGTAC77700.018.70938510-11
GTATAGG2803.479363E-818.6635271
CGGTTAC1152.0606476E-618.58549928-29
ACATGGG37350.018.5661493
TACTTTT55450.018.41710514-15
ACTTTTT57200.018.35083416-17
GTAAGGT2654.0065424E-717.9231244
TCTACGA1350.00940288917.5912152
CATGGGA23400.017.252924
ATCAACG115650.017.1668363
TCAACGC115700.017.1594164
Produced by FastQC (version 0.11.5)