FastQCFastQC Report
Thu 26 May 2016
SRR936911_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936911_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447536
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15070.33673268742626294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12140.2712630939187015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7150.15976368381538023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15350.040.8793071
GGTATCA8900.040.5939751
GTCCTAG1502.5465852E-1134.8609661
ATCAACG19250.031.6094743
TCAACGC19300.031.5275824
TATCAAC19500.031.4480042
CAACGCA19900.030.5735785
AACGCAG20450.029.9837366
ACGCAGA22500.027.2518867
AGACCCG1056.864641E-527.1613485
CGCAGAG22750.026.7404888
CTTAGAC1109.413027E-525.9296463
GTAGTGC1151.2741343E-424.799496
TACATGG11550.024.283322
ACATGGG11600.024.178653
GTACATG12400.023.768841
GCAGAGT24650.023.522519
AGAGTAC21500.022.76366810-11
AGTACTT16300.022.29939312-13
GACCCGG1100.002863304521.6056186
Produced by FastQC (version 0.11.5)