FastQCFastQC Report
Thu 26 May 2016
SRR936911_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936911_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447536
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19680.4397411604876479No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16790.37516534982660615No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10620.23729934575095635No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4830.10792427871724286No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGC608.288145E-539.5788658
GGTATCA12150.037.530821
GTATCAA19550.033.529371
GCGCGCC650.005843532329.227479
CGCGCCA504.9557144E-528.4967810-11
TATCAAC23600.027.5710412
TCAACGC23600.027.5710414
ATCAACG23850.027.2820343
CAACGCA24600.026.4502665
AACGCAG24900.026.1315886
GGGCGCG950.001226190824.9971777
ATACCCT1401.777885E-523.7473186
CGCAGAG27850.023.363618
ACGCAGA27800.023.2347877
GTCTATA1452.3302458E-522.9310071
TACATGG13150.022.7540862
GTACATG13050.022.5670221
GAGTACT17150.022.15493212-13
GTACTAC1302.937647E-421.9230521
ACATGGG12850.021.8068773
Produced by FastQC (version 0.11.5)