##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936911_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 447536 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3284160380394 33.0 31.0 34.0 31.0 34.0 2 32.358002484716316 33.0 31.0 34.0 31.0 34.0 3 32.53547647563548 34.0 31.0 34.0 31.0 34.0 4 36.2183042258053 37.0 35.0 37.0 35.0 37.0 5 36.16296566086304 37.0 35.0 37.0 35.0 37.0 6 36.2116969361124 37.0 36.0 37.0 35.0 37.0 7 36.15932573022059 37.0 36.0 37.0 35.0 37.0 8 36.14532685638697 37.0 36.0 37.0 35.0 37.0 9 37.887325265453505 39.0 38.0 39.0 35.0 39.0 10-11 37.81602709055808 39.0 38.0 39.0 35.0 39.0 12-13 37.731391441135465 39.0 37.0 39.0 35.0 39.0 14-15 39.01800413821458 40.0 38.0 41.0 36.0 41.0 16-17 38.93763183296986 40.0 38.0 41.0 36.0 41.0 18-19 38.985084998748704 40.0 38.5 41.0 35.5 41.0 20-21 39.051224929391154 40.0 38.0 41.0 36.0 41.0 22-23 38.98117134710951 40.0 38.0 41.0 35.0 41.0 24-25 38.872479532372815 40.0 38.0 41.0 35.0 41.0 26-27 38.7225463426406 40.0 38.0 41.0 35.0 41.0 28-29 38.55243600514819 40.0 38.0 41.0 34.0 41.0 30-31 38.430432412141144 40.0 38.0 41.0 34.0 41.0 32-33 38.43809101390726 40.0 38.0 41.0 34.0 41.0 34-35 38.35214708984306 40.0 38.0 41.0 34.0 41.0 36-37 38.180330744342356 40.0 38.0 41.0 34.0 41.0 38-39 38.02967359050445 40.0 38.0 41.0 33.5 41.0 40-41 37.871515364127134 40.0 37.5 41.0 33.0 41.0 42-43 37.650133173644136 40.0 37.0 41.0 33.0 41.0 44-45 37.23360802259483 40.0 37.0 41.0 32.0 41.0 46-47 37.01216661899825 40.0 37.0 41.0 31.5 41.0 48-49 36.682383093203676 40.0 36.5 41.0 31.0 41.0 50-51 36.42117840799399 39.5 36.0 41.0 30.0 41.0 52-53 36.18083461442208 39.0 36.0 41.0 30.0 41.0 54-55 35.95323504701298 39.0 35.0 40.0 29.5 41.0 56-57 35.67989279074756 39.0 35.0 40.0 28.5 41.0 58-59 35.367922357084126 38.0 35.0 40.0 28.0 41.0 60-61 35.043307130599544 38.0 34.5 40.0 27.5 41.0 62-63 34.69040591147974 38.0 34.0 40.0 26.5 41.0 64-65 34.31656894640878 37.0 34.0 40.0 26.0 41.0 66-67 33.93747653820028 36.5 33.5 39.0 26.0 41.0 68-69 33.50232718708663 36.0 33.0 39.0 25.5 40.0 70-71 33.04105591505488 35.5 32.5 38.5 24.5 40.0 72-73 32.695883683100355 35.0 32.0 38.0 23.5 40.0 74-75 32.2483073987344 35.0 32.0 37.0 22.0 39.0 76-77 30.86761623645919 34.0 30.5 36.0 20.5 38.5 78-79 31.56875983161131 34.5 31.5 36.0 22.0 39.0 80-81 31.39100206463838 35.0 31.0 36.0 20.5 37.5 82-83 31.06198830932037 34.5 31.0 35.5 20.0 37.0 84-85 30.683331620249547 34.0 31.0 35.0 18.0 37.0 86-87 30.31705270637446 34.0 31.0 35.0 17.5 36.0 88-89 29.97475063458582 34.0 30.5 35.0 10.5 36.0 90-91 29.5748654856816 34.0 30.0 35.0 6.0 35.5 92-93 29.1507320081513 34.0 29.5 35.0 2.0 35.0 94-95 28.685715785992635 34.0 29.0 35.0 2.0 35.0 96-97 28.03497484001287 34.0 29.0 35.0 2.0 35.0 98-99 27.410913312001718 33.0 27.5 35.0 2.0 35.0 100-101 24.515562993815024 31.0 21.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22522156194427367 1101 2 -0.001836324122841404 1101 3 0.11074014102015184 1101 4 0.10645404187562946 1101 5 0.10675365994323727 1101 6 0.1387760875940387 1101 7 0.15262675570976825 1101 8 0.24296438393282926 1101 9 0.3194984860579808 1101 10-11 0.2634066502457699 1101 12-13 0.277528846291041 1101 14-15 0.32119678523373096 1101 16-17 0.31915168384463044 1101 18-19 0.2818396246846362 1101 20-21 0.19411220606519208 1101 22-23 0.07587650923534994 1101 24-25 0.17917740880297117 1101 26-27 0.10593257125556477 1101 28-29 0.20261492340955556 1101 30-31 0.28362823120816216 1101 32-33 0.4221942249155788 1101 34-35 0.372577785574407 1101 36-37 0.30457116483916735 1101 38-39 0.1690560731778774 1101 40-41 0.17957802026566583 1101 42-43 0.3753027358016823 1101 44-45 0.445982150311373 1101 46-47 0.4258424434804269 1101 48-49 0.3767204285508683 1101 50-51 0.26911376600964587 1101 52-53 0.30993594995999985 1101 54-55 0.43442587813127176 1101 56-57 0.37320910043397504 1101 58-59 0.4212208227115255 1101 60-61 0.41145245080147674 1101 62-63 0.5385836216888364 1101 64-65 0.4716415772868672 1101 66-67 0.5515546093131007 1101 68-69 0.38943072668713086 1101 70-71 0.4969671808250453 1101 72-73 0.5058677255313881 1101 74-75 0.3202076394609108 1101 76-77 0.25429279598553123 1101 78-79 0.5326538380552783 1101 80-81 0.6714834026896632 1101 82-83 0.7973767302197423 1101 84-85 0.6167436082023912 1101 86-87 0.34251509714554373 1101 88-89 0.6221569545523025 1101 90-91 0.5765649654088207 1101 92-93 0.5725748252899727 1101 94-95 0.7034899780823629 1101 96-97 0.6601620452326458 1101 98-99 0.8370006231059115 1101 100-101 1.1361840092981375 1102 1 0.1346201408029657 1102 2 0.06429907150489811 1102 3 0.07457219937275994 1102 4 0.13963842397217974 1102 5 0.02431490611044751 1102 6 -0.006512065026910818 1102 7 0.15065306461440287 1102 8 0.17695770728957427 1102 9 0.13260898555042644 1102 10-11 0.23454167959997818 1102 12-13 0.19441248642205977 1102 14-15 0.2558373662482083 1102 16-17 0.2483630446031313 1102 18-19 0.26074959465019987 1102 20-21 0.1780851227536644 1102 22-23 0.14455384318262787 1102 24-25 0.15036894893085417 1102 26-27 0.11177620385181797 1102 28-29 0.09167485622452176 1102 30-31 0.1594876658911275 1102 32-33 0.1833896370779513 1102 34-35 0.12998174342155266 1102 36-37 0.23592574901406493 1102 38-39 0.2957851458779359 1102 40-41 0.3740807777821189 1102 42-43 0.42102780042280585 1102 44-45 0.5719153629488645 1102 46-47 0.5233005051608828 1102 48-49 0.6996784536039797 1102 50-51 0.7030652295340332 1102 52-53 0.7527679769177382 1102 54-55 0.6731373278419142 1102 56-57 0.6702129829334211 1102 58-59 0.51099341917228 1102 60-61 0.7731831455700444 1102 62-63 0.7550826746101293 1102 64-65 0.6705401404481179 1102 66-67 0.6301145264306314 1102 68-69 0.3652006859784436 1102 70-71 0.5322921700984651 1102 72-73 0.5923857200268188 1102 74-75 0.6225831849468477 1102 76-77 0.6758636908440963 1102 78-79 0.6184068441144852 1102 80-81 0.4627080120851019 1102 82-83 0.5019661375891644 1102 84-85 0.3851493764872913 1102 86-87 0.355576934582885 1102 88-89 0.5661647195512067 1102 90-91 0.7133723159953789 1102 92-93 0.7578872566136887 1102 94-95 0.6105276623964393 1102 96-97 0.624985135116173 1102 98-99 0.5686006105479642 1102 100-101 0.6608514852548986 1103 1 -0.031220741004304386 1103 2 -0.14292563656014323 1103 3 -0.028079770506096224 1103 4 0.1549172627804296 1103 5 0.10108015766561351 1103 6 0.08234529609936203 1103 7 0.08936309408073129 1103 8 0.21609336155127323 1103 9 0.14858628556134335 1103 10-11 -0.009756039487655244 1103 12-13 0.16629942301487688 1103 14-15 0.07747875007446225 1103 16-17 0.26755981422006414 1103 18-19 0.3010966425602746 1103 20-21 0.3218153421141423 1103 22-23 0.23891158627986897 1103 24-25 0.287594748341526 1103 26-27 0.324304851674583 1103 28-29 0.15594420032579848 1103 30-31 0.1935922168024078 1103 32-33 0.4791224756679284 1103 34-35 0.35769100894594885 1103 36-37 0.2767093351758021 1103 38-39 0.2820451044068051 1103 40-41 0.5482158863710112 1103 42-43 0.576173495064296 1103 44-45 0.8211568777365912 1103 46-47 0.6973483060994425 1103 48-49 0.6717836593226281 1103 50-51 0.761845105120635 1103 52-53 0.9010491075518061 1103 54-55 0.7369592202352351 1103 56-57 0.6906538865676026 1103 58-59 0.8476626323120797 1103 60-61 0.7359027788779073 1103 62-63 0.4588914708559031 1103 64-65 0.4343074655599821 1103 66-67 0.7810148865746953 1103 68-69 0.8472371369224518 1103 70-71 0.464443212333542 1103 72-73 0.5854447618253573 1103 74-75 0.5871253654554138 1103 76-77 0.7150757392998415 1103 78-79 0.5465744748031263 1103 80-81 0.8372360051004932 1103 82-83 0.8733728821051514 1103 84-85 0.7821590894633914 1103 86-87 0.4631181328803855 1103 88-89 0.33057593858427126 1103 90-91 0.5643381568771915 1103 92-93 0.7260636180673607 1103 94-95 0.7883951730370562 1103 96-97 0.9308456611447653 1103 98-99 0.6737517619176003 1103 100-101 1.0699314808895117 1104 1 0.07455905045401323 1104 2 -0.11885119499955721 1104 3 -0.014432413036104208 1104 4 0.023365460390081694 1104 5 -0.007642873281625384 1104 6 0.15397525741285278 1104 7 0.028871466988000805 1104 8 0.08149222158628788 1104 9 0.013295508039199433 1104 10-11 0.14534364359342788 1104 12-13 -0.052923781815337634 1104 14-15 0.06623235298862795 1104 16-17 0.10217156399362892 1104 18-19 0.2444206169945673 1104 20-21 0.02965658008231742 1104 22-23 -0.09604498227226799 1104 24-25 -0.11946922290982087 1104 26-27 -0.080390578052139 1104 28-29 -0.08850578916263174 1104 30-31 0.09494457443940263 1104 32-33 0.1979900554330598 1104 34-35 0.1713621402462593 1104 36-37 0.10085568766114505 1104 38-39 -0.04315338742855346 1104 40-41 0.09877948333188868 1104 42-43 0.18782895921394527 1104 44-45 0.09655017744599803 1104 46-47 0.15505348309658729 1104 48-49 0.2637235449331712 1104 50-51 0.007728401155901565 1104 52-53 0.1833525362882753 1104 54-55 0.1844819281727439 1104 56-57 0.41484307124310504 1104 58-59 0.20489488691604407 1104 60-61 0.1904407315385228 1104 62-63 0.2528421116686914 1104 64-65 0.12223762944222472 1104 66-67 0.40948296775151505 1104 68-69 0.21866786960833196 1104 70-71 0.4799207617821253 1104 72-73 0.19682220969485087 1104 74-75 0.2394635511388543 1104 76-77 0.4620711004557627 1104 78-79 0.40403660859281487 1104 80-81 0.47050797182498627 1104 82-83 0.43010883344149775 1104 84-85 0.5579895525815388 1104 86-87 0.5520165400337547 1104 88-89 0.48570312857647124 1104 90-91 0.5538118514286623 1104 92-93 0.6413482261822914 1104 94-95 0.22541515287842273 1104 96-97 0.30162113991237405 1104 98-99 0.2966654118292844 1104 100-101 0.17959957071136756 1105 1 0.1398033572577546 1105 2 -0.018403971606737457 1105 3 -0.01800628003206839 1105 4 0.19403230999692056 1105 5 0.23707227906507455 1105 6 0.15398832456823186 1105 7 0.29965559431435906 1105 8 0.22972276540862424 1105 9 0.11073401890617873 1105 10-11 0.28244587121674414 1105 12-13 0.19422676161499197 1105 14-15 0.2624209904436654 1105 16-17 0.2486576425729652 1105 18-19 0.31275040586200475 1105 20-21 0.28871946903436907 1105 22-23 0.4027316053054406 1105 24-25 0.23980078045497066 1105 26-27 0.14781749026295898 1105 28-29 -0.0061925106460094526 1105 30-31 0.13777707637498082 1105 32-33 0.254677008858458 1105 34-35 0.27261626048368726 1105 36-37 0.4413416551696656 1105 38-39 0.3453854105891523 1105 40-41 -0.23112698850349034 1105 42-43 -0.018650063101489422 1105 44-45 0.22312844480653382 1105 46-47 0.01754944044539286 1105 48-49 0.03920368122359719 1105 50-51 -0.24691288909502163 1105 52-53 -0.004366951087220627 1105 54-55 -0.294123871684171 1105 56-57 -0.0961398728523335 1105 58-59 -0.1395226609669109 1105 60-61 -0.01758184850597644 1105 62-63 -0.13704750353625172 1105 64-65 -0.03689418875443806 1105 66-67 -0.27597916853238047 1105 68-69 -0.2545640625992647 1105 70-71 -0.2608840443176206 1105 72-73 0.007529763490694563 1105 74-75 -0.1769311553565025 1105 76-77 0.05784518240642811 1105 78-79 0.06291035047075866 1105 80-81 0.23416723268134376 1105 82-83 0.13483195675297566 1105 84-85 0.41048586927711384 1105 86-87 0.3191108293601772 1105 88-89 0.19493605388787927 1105 90-91 0.15139573439921605 1105 92-93 0.11658269995161064 1105 94-95 0.38255259648547835 1105 96-97 0.8527904869792913 1105 98-99 0.7523987895909485 1105 100-101 0.3155698560000353 1106 1 0.37259814504068345 1106 2 0.22914621104682453 1106 3 0.15244526969866 1106 4 0.16217903541401313 1106 5 0.06269650231668322 1106 6 0.16626565669643156 1106 7 0.2827083567140889 1106 8 0.26486003803319136 1106 9 0.3899190450802408 1106 10-11 0.4439756418641352 1106 12-13 0.35992555271204196 1106 14-15 0.45218485893310145 1106 16-17 0.4919408874040556 1106 18-19 0.3322660914719151 1106 20-21 0.2174958784796317 1106 22-23 -0.08352606802713325 1106 24-25 0.11411630035856035 1106 26-27 0.2056113365326766 1106 28-29 0.2582069922350172 1106 30-31 -7.38345611246416E-4 1106 32-33 0.038863353151008084 1106 34-35 0.157536985072241 1106 36-37 0.2594450709795595 1106 38-39 0.3241072122688209 1106 40-41 0.28986122375124523 1106 42-43 0.009979470284577019 1106 44-45 0.6449150602548812 1106 46-47 0.4407558577993669 1106 48-49 0.07121513002411461 1106 50-51 0.1720491867532914 1106 52-53 0.44141695280310955 1106 54-55 0.5295796646394066 1106 56-57 0.32438511490799016 1106 58-59 0.2889976568879504 1106 60-61 0.35984443101046537 1106 62-63 0.5612879011261214 1106 64-65 0.7535287389750067 1106 66-67 0.4624119737114327 1106 68-69 0.42196589635075554 1106 70-71 0.23465318311431105 1106 72-73 0.2593920766003208 1106 74-75 0.5023329579263631 1106 76-77 0.48546917969486714 1106 78-79 0.5545384150962214 1106 80-81 0.2909291804838503 1106 82-83 0.57624723939524 1106 84-85 0.5856336868495156 1106 86-87 0.45705004526798376 1106 88-89 0.848093889160733 1106 90-91 0.9662226842730526 1106 92-93 1.0649501160627857 1106 94-95 0.8833961923126914 1106 96-97 1.5830620193861726 1106 98-99 1.243477010507604 1106 100-101 1.7635757310424047 1107 1 0.15283415185584914 1107 2 0.04944027301439036 1107 3 -0.02083673951443643 1107 4 -0.010648629128169773 1107 5 0.10187110949784994 1107 6 0.11761291485255043 1107 7 0.03363913225520321 1107 8 0.0635601074469605 1107 9 0.10501969504553443 1107 10-11 0.23955840200800793 1107 12-13 0.02161546247414492 1107 14-15 -0.015992083074849006 1107 16-17 0.0824248906854379 1107 18-19 0.24708908636053906 1107 20-21 0.12970639151048857 1107 22-23 0.401606764685674 1107 24-25 0.28400334522509496 1107 26-27 0.444621874804902 1107 28-29 0.2899543261152431 1107 30-31 0.2444500181169147 1107 32-33 0.27341878951119725 1107 34-35 0.3342455103451698 1107 36-37 0.33706859593696237 1107 38-39 0.10677901134750556 1107 40-41 0.3846615014063204 1107 42-43 0.34352525182050897 1107 44-45 -0.051923458076664986 1107 46-47 -0.17302593055173787 1107 48-49 -0.29629922600541647 1107 50-51 -0.15437221792898725 1107 52-53 -0.008706098878590751 1107 54-55 -0.11146911430927986 1107 56-57 -0.04267489645062028 1107 58-59 -0.10208582894532725 1107 60-61 0.06744208356300163 1107 62-63 0.0746595172048714 1107 64-65 0.11341389073874808 1107 66-67 0.14402363522465578 1107 68-69 -0.10236678491383344 1107 70-71 -0.010188111768002273 1107 72-73 -0.08592880432350825 1107 74-75 0.15595824977971517 1107 76-77 0.3146292730879381 1107 78-79 -0.05878271824131431 1107 80-81 -0.0033410283884904857 1107 82-83 0.2178765698678795 1107 84-85 0.16572455578369727 1107 86-87 0.08588831118581908 1107 88-89 0.4116264189770966 1107 90-91 0.395269191498393 1107 92-93 0.4860077294911953 1107 94-95 0.7217504549491558 1107 96-97 0.6616510267692739 1107 98-99 1.143779906954883 1107 100-101 0.8363276714727697 1108 1 -0.1673501927850154 1108 2 -0.12536861375102148 1108 3 -0.12299273569217206 1108 4 0.1074732851893927 1108 5 0.14267179432027177 1108 6 -0.12871412767015045 1108 7 0.008505751143559337 1108 8 0.16232185294783363 1108 9 0.01490781417278697 1108 10-11 -0.10027974897145242 1108 12-13 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-0.47893039021106887 2316 92-93 -0.9615585382331808 2316 94-95 -0.8843516116272205 2316 96-97 -0.5580159992218618 2316 98-99 -0.29276707702293336 2316 100-101 -0.52480724104554 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 10.0 6 15.0 7 32.0 8 78.0 9 205.0 10 321.0 11 525.0 12 844.0 13 1170.0 14 1647.0 15 2267.0 16 2979.0 17 4523.0 18 4255.0 19 2443.0 20 1962.0 21 1927.0 22 2067.0 23 2158.0 24 2420.0 25 2796.0 26 3589.0 27 4271.0 28 5481.0 29 6865.0 30 8652.0 31 11236.0 32 14304.0 33 19436.0 34 27957.0 35 40991.0 36 67026.0 37 106040.0 38 88794.0 39 8247.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.65105208461375 20.014750416252276 15.119400149739073 22.214797349394896 2 15.531368636502782 20.94482418056322 40.116371306696934 23.40743587623706 3 19.539287278447638 24.861013040641396 30.43367506547135 25.166024615439618 4 13.260832648099818 17.574228665403453 37.48212434306961 31.682814343427125 5 13.984792046995837 37.2856284173763 34.26615928800145 14.46342024762641 6 31.511948786128457 36.83488442244741 17.408247399644722 14.244919391779415 7 27.689392585177508 33.18414607986844 21.257507775910764 17.868953559043295 8 26.03008473061385 35.342184762790026 19.855162489721497 18.77256801687462 9 26.150298523470738 17.233697400879482 20.7167691537664 35.89923492188338 10-11 24.598828914483498 27.165564142125675 27.57793550650398 20.657671436886847 12-13 25.71095315876148 24.447359189154767 28.252218272172108 21.589469379911648 14-15 22.619074219727576 26.0125442422509 26.612272532265564 24.75610900575596 16-17 21.66551964534697 29.164581173358123 27.191667262521896 21.978231918773012 18-19 21.5461996353366 27.574541489399735 29.914800150155518 20.96445872510815 20-21 22.434986436421003 26.665728165319248 30.0349480025563 20.86433739570345 22-23 22.080681777555323 26.629589574916878 30.02037824890065 21.26935039862715 24-25 22.094667971630443 26.671091030161648 30.142540478456926 21.091700519750983 26-27 21.990168147030893 26.79202279202279 30.102675828166024 21.11513323278029 28-29 21.632522636062173 27.00182786760697 30.16886547073177 21.196784025599086 30-31 21.875310773287744 26.858857248210782 30.04586874053713 21.219963237964347 32-33 22.047473700204677 26.80232921891619 29.968359804438567 21.181837276440568 34-35 22.00334956722653 26.99116576782516 29.868352399499017 21.137132265449292 36-37 22.301399493652784 26.829028488175922 29.716615383927834 21.152956634243463 38-39 21.980562175230904 26.893310943747846 29.873226234248246 21.252900646773007 40-41 22.061099150091003 27.02748601454465 29.653857885204744 21.257556950159604 42-43 21.936438949996926 27.211480986989482 29.394276265439167 21.457803797574424 44-45 22.262194311276563 27.077018643255435 29.29159651378832 21.36919053167968 46-47 22.39824818170646 27.31562894522216 28.81070754242685 21.47541533064453 48-49 22.51628919237782 27.520579350041114 28.558484680562014 21.404646777019057 50-51 22.38827981243946 27.746960855619275 28.332724443088875 21.53203488885239 52-53 22.35910968898105 27.96422131303223 28.07371142428759 21.602957573699136 54-55 22.29131914922143 27.861467489126174 28.084139350352334 21.763074011300066 56-57 22.270896527067865 28.104439342394127 28.096953852513508 21.5277102780245 58-59 22.33948418639364 27.833801834977184 28.095239159289072 21.731474819340104 60-61 22.241215383566892 27.756605989102397 28.465832978608113 21.536345648722598 62-63 22.182908190626005 27.74882914447106 28.530665689464087 21.53759697543885 64-65 22.158403430350063 27.814615409538256 28.39881618065199 21.628164979459687 66-67 22.044867999150906 27.874132749393905 28.277564883193495 21.8034343682617 68-69 22.169389725072396 27.681460262414642 28.346881591648494 21.80226842086447 70-71 22.07221550022289 27.88650285843246 28.135086490345458 21.906195150999196 72-73 22.109617997211398 27.795305852490078 28.11103464302313 21.984041507275393 74-75 22.191088751618587 28.027720705955172 27.785058392015827 21.996132150410414 76-77 22.141520448235603 28.114271030595546 27.776195024942325 21.968013496226533 78-79 22.316765113673053 27.951315376390816 27.65714745376603 22.074772056170097 80-81 22.24001215624801 28.090653431849592 27.663061877313517 22.006272534588884 82-83 22.224357413072283 28.192042044390618 27.665481635744882 21.918118906792216 84-85 22.17150990975691 27.995915207027274 27.55637539426185 22.276199488953967 86-87 22.164013183621027 27.904586335958886 27.625272331154683 22.306128149265405 88-89 22.075937148066714 27.90473445236946 27.62296437317889 22.39636402638494 90-91 22.029325211772093 28.02128558117502 27.675165853691237 22.274223353361652 92-93 21.95801008187051 27.908481105430628 27.72335633334525 22.410152479353616 94-95 21.80630053392651 28.29654092933825 27.55134531828344 22.345813218451806 96-97 21.921645126115013 28.329735793067695 27.428563129010925 22.320055951806367 98-99 21.732669672135728 28.305932170250326 27.1677643891021 22.793633768511846 100-101 21.396801775078515 28.709839239647888 26.914091696748482 22.979267288525115 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 91.0 1 69.5 2 55.0 3 70.0 4 63.5 5 66.0 6 110.0 7 204.5 8 354.5 9 470.0 10 475.5 11 467.5 12 513.5 13 563.5 14 637.0 15 697.0 16 731.0 17 722.0 18 752.0 19 849.0 20 929.0 21 1048.0 22 1216.5 23 1478.5 24 1859.0 25 2339.0 26 2884.5 27 3545.5 28 4200.5 29 4949.0 30 5884.0 31 6664.5 32 7516.0 33 8364.0 34 9417.0 35 10266.5 36 11154.0 37 12336.5 38 13092.0 39 13565.0 40 13932.0 41 14847.5 42 15496.0 43 16008.0 44 16816.0 45 17126.5 46 17270.0 47 17554.5 48 18052.5 49 17441.5 50 16254.0 51 15496.5 52 14868.0 53 14328.5 54 13444.0 55 12434.5 56 11094.5 57 9844.0 58 9068.0 59 8041.5 60 6476.0 61 5148.5 62 4180.5 63 3214.5 64 2357.5 65 1682.5 66 1261.0 67 1006.5 68 717.0 69 436.0 70 291.0 71 209.0 72 150.0 73 118.0 74 88.0 75 52.0 76 34.0 77 22.0 78 13.0 79 10.0 80 8.5 81 6.0 82 3.5 83 3.5 84 2.5 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02033355975832112 2 6.703371348897072E-4 3 0.0026813485395588287 4 0.0 5 0.0015641199814093168 6 2.234457116299024E-4 7 0.0 8 0.0 9 0.0 10-11 5.586142790747561E-4 12-13 6.703371348897072E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0031282399628186335 22-23 0.0 24-25 0.0031282399628186335 26-27 0.0024579028279289265 28-29 0.0044689142325980485 30-31 0.014188802688498803 32-33 8.937828465196096E-4 34-35 0.003463408530263487 36-37 0.0037985770977083406 38-39 0.0014523971255943654 40-41 0.003910299953523292 42-43 0.0030165171070036826 44-45 3.351685674448536E-4 46-47 2.234457116299024E-4 48-49 0.0 50-51 1.117228558149512E-4 52-53 0.0015641199814093168 54-55 0.0036868542418933893 56-57 7.820599907046584E-4 58-59 0.0022344571162990243 60-61 0.0014523971255943654 62-63 0.0 64-65 3.351685674448536E-4 66-67 2.234457116299024E-4 68-69 0.0 70-71 1.117228558149512E-4 72-73 0.0 74-75 1.117228558149512E-4 76-77 7.820599907046584E-4 78-79 5.586142790747561E-4 80-81 0.006591648493082121 82-83 0.0020110114046691216 84-85 0.005027528511672804 86-87 0.0024579028279289265 88-89 0.0017875656930392192 90-91 6.703371348897072E-4 92-93 0.0 94-95 5.586142790747561E-4 96-97 0.0017875656930392192 98-99 0.004133745665153194 100-101 0.0014523971255943654 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 447536.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.39014168011911 #Duplication Level Percentage of deduplicated Percentage of total 1 87.75969200954898 67.03975306413064 2 7.773289311106485 11.8760534359196 3 2.039960274082236 4.674985630768699 4 0.8373389483255151 2.558577635874722 5 0.44037318696106326 1.6820085072040605 6 0.2696196762568432 1.235777116140486 7 0.18675869851295365 0.9986566407579427 8 0.13272155632121055 0.8110894793106547 9 0.09944918644040836 0.6837243697939838 >10 0.43410917587136916 5.552711776780059 >50 0.01906284060558756 1.0005304044998728 >100 0.006745312586571868 0.8311615979355804 >500 0.0 0.0 >1k 8.798233808571972E-4 1.0549703408837303 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1968 0.4397411604876479 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1679 0.37516534982660615 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1062 0.23729934575095635 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 483 0.10792427871724286 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.234457116299024E-4 0.0 22-23 0.0 0.0 0.0 5.58614279074756E-4 0.0 24-25 0.0 0.0 0.0 0.0010055057023345608 0.0 26-27 0.0 0.0 0.0 0.0017875656930392192 0.0 28-29 0.0 0.0 0.0 0.0036868542418933897 0.0 30-31 0.0 0.0 0.0 0.007038539916341926 0.0 32-33 1.117228558149512E-4 0.0 0.0 0.011284008437310072 0.0 34-35 2.234457116299024E-4 0.0 0.0 0.016311536948982876 0.0 36-37 2.234457116299024E-4 0.0 0.0 0.021674234028100533 0.0 38-39 2.234457116299024E-4 0.0 0.0 0.030723785349111583 0.0 40-41 2.234457116299024E-4 0.0 0.0 0.04301329948875621 0.0 42-43 2.234457116299024E-4 0.0 0.0 0.06211790783311287 0.0 44-45 2.234457116299024E-4 0.0 0.0 0.07831772192628078 0.0 46-47 2.234457116299024E-4 0.0 0.0 0.1066953273032784 0.0 48-49 2.234457116299024E-4 0.0 0.0 0.14233491830824782 0.0 50-51 2.234457116299024E-4 0.0 0.0 0.15764094955489616 0.0 52-53 2.234457116299024E-4 0.0 0.0 0.1806558578527761 0.0 54-55 2.234457116299024E-4 0.0 0.0 0.21875335168567445 0.0 56-57 2.234457116299024E-4 0.0 0.0 0.2806478138071574 0.0 58-59 2.234457116299024E-4 0.0 0.0 0.36801508705444924 0.0 60-61 2.234457116299024E-4 0.0 0.0 0.46778359729720065 0.0 62-63 2.234457116299024E-4 0.0 0.0 0.5693396732329914 0.0 64-65 2.234457116299024E-4 0.0 0.0 0.6800570233456079 0.0 66-67 2.234457116299024E-4 0.0 0.0 0.823956061635265 0.0 68-69 2.234457116299024E-4 0.0 0.0 0.9861776482785742 0.0 70-71 2.234457116299024E-4 0.0 0.0 1.0821475814236172 0.0 72-73 2.234457116299024E-4 0.0 0.0 1.2095116370526617 0.0 74-75 2.234457116299024E-4 0.0 0.0 1.3405625469235996 0.0 76-77 2.234457116299024E-4 0.0 0.0 1.481668513817883 0.0 78-79 2.234457116299024E-4 0.0 0.0 1.6285840692145437 0.0 80-81 2.234457116299024E-4 0.0 0.0 1.8875576489936003 0.0 82-83 2.234457116299024E-4 0.0 0.0 2.16351310285653 4.468914232598048E-4 84-85 2.234457116299024E-4 0.0 0.0 2.6376649029351826 4.468914232598048E-4 86-87 2.234457116299024E-4 0.0 0.0 3.1923688820564156 4.468914232598048E-4 88-89 2.234457116299024E-4 0.0 0.0 3.7414867183869007 4.468914232598048E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGCGC 60 8.288145E-5 39.578865 8 GGTATCA 1215 0.0 37.53082 1 GTATCAA 1955 0.0 33.52937 1 GCGCGCC 65 0.0058435323 29.22747 9 CGCGCCA 50 4.9557144E-5 28.49678 10-11 TATCAAC 2360 0.0 27.571041 2 TCAACGC 2360 0.0 27.571041 4 ATCAACG 2385 0.0 27.282034 3 CAACGCA 2460 0.0 26.450266 5 AACGCAG 2490 0.0 26.131588 6 GGGCGCG 95 0.0012261908 24.997177 7 ATACCCT 140 1.777885E-5 23.747318 6 CGCAGAG 2785 0.0 23.36361 8 ACGCAGA 2780 0.0 23.234787 7 GTCTATA 145 2.3302458E-5 22.931007 1 TACATGG 1315 0.0 22.754086 2 GTACATG 1305 0.0 22.567022 1 GAGTACT 1715 0.0 22.154932 12-13 GTACTAC 130 2.937647E-4 21.923052 1 ACATGGG 1285 0.0 21.806877 3 >>END_MODULE