FastQCFastQC Report
Thu 26 May 2016
SRR936910_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936910_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280672
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10600.3776650324934443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7850.27968589670505073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4880.17386843005358568No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7550.044.0641331
GTATCAA11300.034.067541
GTAGGAC600.003931779431.6898633
ATCAACG13750.027.6566093
TCAACGC14000.027.162744
CTACACG350.00841642727.1240238-39
TATCAAC14150.026.8700032
CAACGCA14450.025.992525
AACGCAG14500.025.9028916
GAGTACT9950.022.69451712-13
ACGCAGA16400.022.612057
CGCAGAG16250.022.5282028
GTACATG8900.021.8941461
CTCCTAA1604.9508693E-520.7927631
GCAGAGT17650.020.741269
TACATGG9500.020.5113562
ACATGGG9100.020.3720553
AGTACTT10700.020.21519912-13
GTACTTT11350.019.89341514-15
CAGAGTA17150.019.68264210-11
Produced by FastQC (version 0.11.5)