FastQCFastQC Report
Thu 26 May 2016
SRR936910_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936910_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280672
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12590.44856629802759096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10730.3822967734579865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6330.2255301561965568No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.10367974005244555No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAG250.00501982956.9928361
GGTATCA8700.044.764491
ACTATAC651.3279995E-436.5273633
GTATCAA13850.033.6058851
CCTATAC600.003951783831.6570433
GTATAGT804.4883857E-429.683771
GTACCCC650.00584531929.221896
CTATACA1354.217145E-728.1395974
ATAGTAC1056.9018206E-527.1346113
TCAACGC17300.026.899344
CTATAGA1606.2142135E-826.7153931
ATCAACG17450.026.6681143
AACGCAG17600.026.1710246
TATCAAC17850.026.0751532
CAACGCA17550.025.9750125
TACTATA1109.459701E-525.9058362
ACTAGTA1109.471172E-525.901228
TTAGTAC950.00122641624.9924053
TAGTACC950.00122641624.9924054
TAAGGTG1351.3411836E-524.6221475
Produced by FastQC (version 0.11.5)