FastQCFastQC Report
Thu 26 May 2016
SRR936909_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936909_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302185
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10990.3636844979069113No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8190.2710260271026027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4820.15950493902741697No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6550.039.1834531
GTATCAA10900.038.371291
ATCAACG13450.030.748163
TCAACGC13550.030.5212334
CAACGCA13850.029.8601255
TATCAAC13950.029.6460742
AACGCAG14350.029.1461356
TGTGCCG700.00835107227.1633975
ACGCAGA16400.025.502877
CGCAGAG16450.024.8433888
TACATGG8900.023.5009172
GCAGAGT16950.022.7050089
GTACATG9450.022.1294731
TATAGAG1100.00285992521.6072485
AGTACTT10100.021.40229812-13
ACATGGG9600.021.2921433
GTGGTAT4300.021.0007781
AGAGTAC14600.020.82732410-11
GTCCTAA1150.003704312520.6643811
GAGTACT9600.020.53748712-13
Produced by FastQC (version 0.11.5)