FastQCFastQC Report
Thu 26 May 2016
SRR936909_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936909_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302185
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13860.4586594304813276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12160.4024025017787117No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6970.2306534076807254No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.11549216539537038No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGCG500.001612135138.008619
GGTATCA10050.036.3952561
TACGCGT550.002573766734.553285
GTATCAA16350.033.9928971
CGCGTAT702.0470363E-433.936267
ACGCGTA753.0690347E-431.6738416
CGCAATA300.00395470731.65811518-19
ATACGCG600.00395160531.6581154
TCAACGC19100.028.5917554
AGGCGTG1004.9335657E-528.5064567
ATCAACG19200.028.4428373
CAACGCA19900.027.6947155
TATCAAC19800.027.5809332
AACGCAG20250.027.2160426
AAGGCGT1109.4394156E-525.914966
AAAGGCG1151.2767327E-424.7882235
TTAGTAC1151.2810461E-424.7759173
ACGCAGA22600.023.9656057
GAGTACT13600.023.91817312-13
CGCAGAG22450.023.91418
Produced by FastQC (version 0.11.5)