##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936909_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 302185 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47086387477869 33.0 31.0 34.0 31.0 34.0 2 32.60962324403925 34.0 31.0 34.0 31.0 34.0 3 32.70913844168307 34.0 31.0 34.0 31.0 34.0 4 36.27512285520459 37.0 35.0 37.0 35.0 37.0 5 36.20828300544368 37.0 35.0 37.0 35.0 37.0 6 36.25194830981022 37.0 37.0 37.0 35.0 37.0 7 36.215798269272135 37.0 37.0 37.0 35.0 37.0 8 36.19483429025266 37.0 36.0 37.0 35.0 37.0 9 37.93164783162632 39.0 38.0 39.0 35.0 39.0 10-11 37.85295266144911 39.0 38.0 39.0 35.0 39.0 12-13 37.77933219716399 39.0 37.0 39.0 35.0 39.0 14-15 39.07352946042987 40.0 38.0 41.0 36.0 41.0 16-17 38.99201647997088 40.0 38.0 41.0 36.0 41.0 18-19 39.11487995764185 40.0 38.5 41.0 36.0 41.0 20-21 39.133625097208665 40.0 39.0 41.0 36.0 41.0 22-23 39.047922630176885 40.0 38.5 41.0 35.5 41.0 24-25 38.948945182586826 40.0 38.0 41.0 35.0 41.0 26-27 38.78637258632956 40.0 38.0 41.0 35.0 41.0 28-29 38.711305988053674 40.0 38.0 41.0 35.0 41.0 30-31 38.726473848801234 40.0 38.0 41.0 35.0 41.0 32-33 38.57400102586164 40.0 38.0 41.0 35.0 41.0 34-35 38.414113870642154 40.0 38.0 41.0 34.0 41.0 36-37 38.27695286000298 40.0 38.0 41.0 34.0 41.0 38-39 38.10929066631368 40.0 38.0 41.0 34.0 41.0 40-41 37.95002564654103 40.0 38.0 41.0 33.5 41.0 42-43 37.71889074573522 40.0 37.0 41.0 33.0 41.0 44-45 37.44074656253619 40.0 37.0 41.0 33.0 41.0 46-47 37.06324933401724 40.0 37.0 41.0 31.5 41.0 48-49 36.679057530982675 40.0 37.0 41.0 31.0 41.0 50-51 36.368961066896105 39.5 36.0 41.0 30.0 41.0 52-53 36.11742310174231 39.0 36.0 40.5 30.0 41.0 54-55 35.80943627248209 39.0 35.0 40.0 29.0 41.0 56-57 35.53498850042193 39.0 35.0 40.0 28.0 41.0 58-59 35.22458924168969 38.0 35.0 40.0 28.0 41.0 60-61 34.87553319986101 38.0 34.0 40.0 27.0 41.0 62-63 34.53264556480302 38.0 34.0 40.0 26.0 41.0 64-65 34.169291990006116 37.0 34.0 40.0 26.0 41.0 66-67 33.77959528103645 36.5 33.0 39.0 26.0 40.5 68-69 33.46169730463127 36.0 33.0 39.0 25.0 40.0 70-71 33.00299981799229 35.5 33.0 39.0 23.5 40.0 72-73 32.484423449211576 35.0 32.0 38.0 22.0 40.0 74-75 31.999086652216356 35.0 31.5 37.0 21.0 39.0 76-77 30.59310190777173 33.5 29.5 35.5 20.0 38.5 78-79 31.25784039578404 34.5 31.0 36.0 20.0 39.0 80-81 30.891483693763753 34.0 31.0 36.0 16.0 37.5 82-83 30.630866853086687 34.0 31.0 35.5 16.5 37.0 84-85 30.347373297814254 34.0 31.0 35.0 14.5 37.0 86-87 30.051437033605243 34.0 30.5 35.0 10.0 36.0 88-89 29.697974750566708 34.0 30.0 35.0 7.0 36.0 90-91 29.303747704220925 34.0 29.5 35.0 2.0 35.5 92-93 28.8623525323891 34.0 29.0 35.0 2.0 35.0 94-95 28.297769578238494 34.0 29.0 35.0 2.0 35.0 96-97 27.65335804225888 33.0 27.5 35.0 2.0 35.0 98-99 26.90629084832139 33.0 26.5 34.5 2.0 35.0 100-101 23.505902013667125 29.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.024628132851852058 1101 2 0.02667422873083325 1101 3 -0.013594160240934627 1101 4 0.08837909404673638 1101 5 0.11162506608060596 1101 6 0.12900469841873274 1101 7 0.12635255587172622 1101 8 0.0927372014223593 1101 9 0.10844401168852613 1101 10-11 0.09093143376395574 1101 12-13 0.09838008739664161 1101 14-15 0.1303824908892608 1101 16-17 0.21299453931607815 1101 18-19 0.0897766714487247 1101 20-21 0.053714377844663375 1101 22-23 0.06522960284633683 1101 24-25 0.13003293439908958 1101 26-27 0.02891432883028955 1101 28-29 0.26301218808124105 1101 30-31 0.30192123954900296 1101 32-33 0.1367933034739579 1101 34-35 0.32243976767247773 1101 36-37 0.2735326158010025 1101 38-39 0.2379348658016056 1101 40-41 0.2854465054524056 1101 42-43 0.34137547236603183 1101 44-45 0.28297788512170996 1101 46-47 0.2855582549459683 1101 48-49 0.4973663936932482 1101 50-51 0.3223264470328786 1101 52-53 0.13015790923257242 1101 54-55 0.0999783741925242 1101 56-57 0.09111348229604488 1101 58-59 0.04815909650848482 1101 60-61 0.3092238271032741 1101 62-63 0.12231169708574186 1101 64-65 0.08472010622845261 1101 66-67 -0.3761203764539687 1101 68-69 -0.32169095632993105 1101 70-71 0.09982775373884323 1101 72-73 0.19364457665144386 1101 74-75 0.2135750361590638 1101 76-77 -0.05447098677020179 1101 78-79 0.07417332053656267 1101 80-81 0.3857624359116585 1101 82-83 0.25591239745546446 1101 84-85 0.22133954458306704 1101 86-87 -0.17479998618573944 1101 88-89 -0.19299798576228255 1101 90-91 -0.0587576526786755 1101 92-93 -0.20427625851336373 1101 94-95 -0.5502230436282396 1101 96-97 -0.32677590275548596 1101 98-99 -0.1706908842098933 1101 100-101 0.08255376235874934 1102 1 0.20723063310546763 1102 2 0.2886653252045761 1102 3 0.2548587164190437 1102 4 0.12463017631824869 1102 5 0.21848389541081303 1102 6 0.16616406304573417 1102 7 0.1570878871116861 1102 8 0.19372876265356354 1102 9 0.20505694421376575 1102 10-11 0.21369346142763845 1102 12-13 0.25204966607158497 1102 14-15 0.417481106761322 1102 16-17 0.4442974880488464 1102 18-19 0.16782171753520458 1102 20-21 0.24260761810067777 1102 22-23 0.24733667636058243 1102 24-25 0.3316834131936517 1102 26-27 0.26852511214715236 1102 28-29 0.28021175304937884 1102 30-31 0.3318910185179078 1102 32-33 0.33255174283254263 1102 34-35 0.259161977181364 1102 36-37 0.22958000690738345 1102 38-39 0.2675484093531537 1102 40-41 0.39491812103900514 1102 42-43 0.404586026371085 1102 44-45 0.6221906756246156 1102 46-47 0.3802944637990109 1102 48-49 0.45381956289091363 1102 50-51 0.7671288307432462 1102 52-53 0.5355490958268021 1102 54-55 0.5223674162967171 1102 56-57 0.6956355488389363 1102 58-59 0.8969619331804495 1102 60-61 0.8995023748895434 1102 62-63 0.9190074366516967 1102 64-65 0.4566003922548205 1102 66-67 0.40709031910158444 1102 68-69 0.5962062166707298 1102 70-71 0.5490299987297149 1102 72-73 0.5459849961599375 1102 74-75 0.6278780061244085 1102 76-77 0.69132319408887 1102 78-79 0.5321446188141259 1102 80-81 0.5408439807937846 1102 82-83 0.629202290514062 1102 84-85 0.7266559453313981 1102 86-87 0.9057162956601736 1102 88-89 0.5530073412547516 1102 90-91 0.7250782242384481 1102 92-93 0.5750878022872925 1102 94-95 0.40216290795677523 1102 96-97 0.1337480228172616 1102 98-99 0.6037271663094792 1102 100-101 0.9875049628249215 1103 1 0.27252062488953754 1103 2 0.15463921681520532 1103 3 0.2623557495566118 1103 4 0.09495774835975368 1103 5 0.19157673538282438 1103 6 0.13268796530662996 1103 7 0.07674677400576257 1103 8 0.10832496506965583 1103 9 0.1901579696831348 1103 10-11 0.24884697753998353 1103 12-13 0.23646222731066757 1103 14-15 0.36308672706378786 1103 16-17 0.34685887927910386 1103 18-19 0.304379168163571 1103 20-21 0.25553819351984686 1103 22-23 0.28475147415749547 1103 24-25 0.3529193015787584 1103 26-27 0.44052705962607064 1103 28-29 0.35099994950131475 1103 30-31 0.3030306740575668 1103 32-33 0.6011535898945866 1103 34-35 0.7286584473758069 1103 36-37 0.5995059114200529 1103 38-39 0.4494303890872047 1103 40-41 0.6477499531906616 1103 42-43 0.8140168452251189 1103 44-45 1.0544302003180448 1103 46-47 1.0247102961941081 1103 48-49 1.2650491371841923 1103 50-51 1.3915944151390747 1103 52-53 1.2725747782051613 1103 54-55 1.2635733317639577 1103 56-57 0.9861027916347851 1103 58-59 1.1126566354291754 1103 60-61 1.1732505871647518 1103 62-63 1.5246619721475483 1103 64-65 1.3219790095447834 1103 66-67 1.03511233474228 1103 68-69 0.9152561360329301 1103 70-71 0.8429414645119024 1103 72-73 0.9389326120197126 1103 74-75 1.011162398906567 1103 76-77 0.706638670460471 1103 78-79 0.9776037978300387 1103 80-81 1.0402410991335564 1103 82-83 0.7120709421892002 1103 84-85 0.6787620372889158 1103 86-87 0.7901379857067319 1103 88-89 0.8492732786311343 1103 90-91 0.7707165561010321 1103 92-93 0.41424392010976874 1103 94-95 0.3158861681582188 1103 96-97 0.4905265427332779 1103 98-99 0.3012308178324723 1103 100-101 0.6946623282510842 1104 1 0.1409639790376218 1104 2 0.19977748621752056 1104 3 0.03722194045759153 1104 4 0.028253912498911404 1104 5 0.12114854626596383 1104 6 0.09524371909789409 1104 7 0.18990466874260648 1104 8 0.10620632100185645 1104 9 0.055746729718819665 1104 10-11 0.3808719552384616 1104 12-13 0.07049434149443812 1104 14-15 0.1035082257256974 1104 16-17 0.26238073476528 1104 18-19 0.1603002207951505 1104 20-21 0.2525386395501812 1104 22-23 0.025797415281495262 1104 24-25 0.06291038097216273 1104 26-27 0.2226189418740674 1104 28-29 0.1043117247020291 1104 30-31 0.059643073700740956 1104 32-33 -0.17956674908845827 1104 34-35 -0.1820574313039387 1104 36-37 -0.4318723222998315 1104 38-39 -0.26108477594402046 1104 40-41 0.03553987290521121 1104 42-43 -0.11803043398987256 1104 44-45 0.23220673912268097 1104 46-47 -0.027597910763965672 1104 48-49 -0.1344044756614764 1104 50-51 0.17148959801150454 1104 52-53 -0.09426286675469697 1104 54-55 -0.03537849696039075 1104 56-57 -0.28867419320018683 1104 58-59 -0.15449323076173016 1104 60-61 -0.0324474503017953 1104 62-63 0.022097297749688494 1104 64-65 0.09841745735923979 1104 66-67 0.09564980129000134 1104 68-69 0.10170796475728139 1104 70-71 -0.23079849980566536 1104 72-73 0.014434396141034256 1104 74-75 -0.14505972419780733 1104 76-77 -0.06531717253685088 1104 78-79 0.11511494858829607 1104 80-81 0.04721032304078321 1104 82-83 0.3371489974969606 1104 84-85 0.33695784460381617 1104 86-87 -0.10031696522993272 1104 88-89 0.41583215463470324 1104 90-91 0.2332611591340381 1104 92-93 0.08504008424892717 1104 94-95 0.1629089157050494 1104 96-97 0.16279460696164705 1104 98-99 0.20893599178429767 1104 100-101 0.5954540320869448 1105 1 0.25746865536257957 1105 2 0.1441567270024109 1105 3 0.26163969215390637 1105 4 0.14588640837274625 1105 5 0.15949441092761418 1105 6 0.2124360902697262 1105 7 -0.01645880090713092 1105 8 0.12798332448618055 1105 9 0.2838138487358748 1105 10-11 0.14205845808093898 1105 12-13 0.17482223439742484 1105 14-15 0.3039529350600034 1105 16-17 -0.053963479428738026 1105 18-19 0.03891749568970937 1105 20-21 0.27305945378087415 1105 22-23 0.3141799570016488 1105 24-25 0.07348712771707255 1105 26-27 0.431875314512979 1105 28-29 0.3579427534935178 1105 30-31 0.6110276326591162 1105 32-33 0.4659036341868017 1105 34-35 0.4563388643131532 1105 36-37 0.5119475933429314 1105 38-39 0.38833297575229864 1105 40-41 0.517623129828003 1105 42-43 0.824359076371401 1105 44-45 0.6617317712151021 1105 46-47 0.6640351352657419 1105 48-49 0.0665720510210761 1105 50-51 0.28585783567884704 1105 52-53 0.4948257083415797 1105 54-55 0.7203293702686508 1105 56-57 0.4544855353895869 1105 58-59 0.5238581354587026 1105 60-61 0.7232970939857495 1105 62-63 0.6911785374342685 1105 64-65 0.31410606913257055 1105 66-67 0.4461403569474385 1105 68-69 0.712202188120564 1105 70-71 0.31828915836414495 1105 72-73 -0.03344750373059924 1105 74-75 0.371811722712156 1105 76-77 0.32451869762635965 1105 78-79 0.43414575731743454 1105 80-81 0.25505462892745 1105 82-83 0.9080824286357831 1105 84-85 0.7852294382220073 1105 86-87 0.5996371884469411 1105 88-89 0.6326806783830996 1105 90-91 0.5278512930053019 1105 92-93 0.8038049843039445 1105 94-95 0.47471434390970657 1105 96-97 0.9916227749425737 1105 98-99 0.4539983427837484 1105 100-101 1.2138888386154605 1106 1 0.23770361573999566 1106 2 0.16288880433582875 1106 3 0.24227550356891214 1106 4 0.2967914066768387 1106 5 0.32068278008058826 1106 6 0.10593305305224732 1106 7 0.21612730497921717 1106 8 0.09956915286026202 1106 9 0.13625441486087908 1106 10-11 0.3135687414517889 1106 12-13 0.26721068988753416 1106 14-15 0.30261162581884804 1106 16-17 0.2347957324364458 1106 18-19 0.027277398252060436 1106 20-21 0.13090380023950843 1106 22-23 -0.26498041424807894 1106 24-25 0.18299654771645635 1106 26-27 0.12978009697420845 1106 28-29 0.28244400229867495 1106 30-31 0.4333272842270617 1106 32-33 0.20981378188499633 1106 34-35 0.40181541452745506 1106 36-37 0.394559908720197 1106 38-39 0.5525971057513672 1106 40-41 0.5087350289368757 1106 42-43 0.42128023205577136 1106 44-45 0.2827656585451237 1106 46-47 0.5851291502513973 1106 48-49 0.7073863953212864 1106 50-51 1.022490995310065 1106 52-53 1.0881777480566726 1106 54-55 0.6105809813814815 1106 56-57 0.908950399254401 1106 58-59 1.0086180873565809 1106 60-61 1.0590406726604797 1106 62-63 1.3339277588582092 1106 64-65 1.2654412114346911 1106 66-67 1.3639427682205962 1106 68-69 1.0920316722561196 1106 70-71 1.2607361997751454 1106 72-73 0.915505658367401 1106 74-75 1.0414796330448617 1106 76-77 1.0625240011939354 1106 78-79 1.190836886175628 1106 80-81 1.1543947664501921 1106 82-83 1.471848578307391 1106 84-85 1.7784226792568205 1106 86-87 1.4105819635078412 1106 88-89 1.719989117839173 1106 90-91 1.9383612988638923 1106 92-93 2.2007014722321614 1106 94-95 1.9982489250203734 1106 96-97 2.349636712224804 1106 98-99 2.2111134487009245 1106 100-101 1.4708266450352454 1107 1 0.1814780549250159 1107 2 -0.19226657742996878 1107 3 -0.0291795283306584 1107 4 0.12305195411457959 1107 5 0.07378012521333233 1107 6 0.08037223806974225 1107 7 0.1988397972615772 1107 8 3.923675134842597E-4 1107 9 0.16302947145688762 1107 10-11 0.0198560433657633 1107 12-13 0.15666325214314725 1107 14-15 0.0780798291528697 1107 16-17 0.1794859979231731 1107 18-19 0.2453675402565949 1107 20-21 0.22867083049264636 1107 22-23 0.005785175379408258 1107 24-25 0.11061539933201203 1107 26-27 0.21340841555827694 1107 28-29 0.3172248213972537 1107 30-31 0.200769144692174 1107 32-33 0.27508441370223835 1107 34-35 0.2403109897486928 1107 36-37 0.5417506887160783 1107 38-39 0.6789091041049389 1107 40-41 0.9656500320239374 1107 42-43 1.154980581921997 1107 44-45 0.8457930304323469 1107 46-47 0.9749418689177887 1107 48-49 0.9595061730533345 1107 50-51 1.2560061218793237 1107 52-53 1.2620406828169095 1107 54-55 1.1293399852917503 1107 56-57 0.6599794175709306 1107 58-59 0.7057882869861345 1107 60-61 1.1491620968218257 1107 62-63 0.8619993785330422 1107 64-65 0.9142681305538503 1107 66-67 0.5971491892948961 1107 68-69 0.5951290173888637 1107 70-71 0.35485021500461045 1107 72-73 0.8887306017713854 1107 74-75 0.9271556980788098 1107 76-77 0.5746767075121078 1107 78-79 0.9166755361035932 1107 80-81 1.3830426363822745 1107 82-83 0.7137707845226622 1107 84-85 1.1584082730003864 1107 86-87 1.4367087385644375 1107 88-89 1.0193511264829276 1107 90-91 0.6232244394277977 1107 92-93 1.2176288235390125 1107 94-95 1.2229762351664952 1107 96-97 1.4484739215271283 1107 98-99 1.429162429972795 1107 100-101 1.489976675905794 1108 1 -0.026484533548192246 1108 2 0.022546364936054886 1108 3 -0.014265473730048939 1108 4 -0.21104814699765484 1108 5 -0.20774161391710777 1108 6 -0.08765101774421424 1108 7 -0.1080358346898862 1108 8 -0.38292411378075286 1108 9 -0.026404300029462036 1108 10-11 -0.23940836512767305 1108 12-13 -0.292692157361401 1108 14-15 -0.0049585934962763645 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-0.30856081535175406 2316 78-79 -0.36449770664529524 2316 80-81 -0.42980032154821757 2316 82-83 0.4121637816483492 2316 84-85 -0.21879319028678523 2316 86-87 -0.14425546316015314 2316 88-89 -0.31963502454921056 2316 90-91 -0.5548523831486349 2316 92-93 -0.691438330890044 2316 94-95 -0.3535458447916966 2316 96-97 0.6642434537978374 2316 98-99 0.29322043885703764 2316 100-101 0.24744989252455696 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 1.0 5 3.0 6 10.0 7 23.0 8 52.0 9 125.0 10 231.0 11 369.0 12 583.0 13 855.0 14 1012.0 15 1491.0 16 2117.0 17 3133.0 18 3103.0 19 1825.0 20 1475.0 21 1425.0 22 1452.0 23 1517.0 24 1783.0 25 2035.0 26 2446.0 27 2935.0 28 3663.0 29 4489.0 30 5701.0 31 7505.0 32 9771.0 33 13176.0 34 18803.0 35 28551.0 36 46212.0 37 73902.0 38 56357.0 39 4050.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.742330557284156 20.125584228156818 15.053557006103777 22.078528208455253 2 15.560799004553639 20.97949539341311 40.08557926506407 23.374126336969184 3 19.46363804834141 24.956648929129273 30.46587509596802 25.1138379265613 4 13.163128547082085 17.557456524976423 37.7010771547231 31.57833777321839 5 13.822658305342753 37.474725747472576 34.27701573539388 14.42560021179079 6 31.37746744543905 37.13619140592683 17.26492049572282 14.221420652911295 7 27.52287505997981 33.51489981302844 21.169482270794383 17.792742856197364 8 25.956615980276982 35.40678723298642 19.886824296374737 18.749772490361867 9 26.11479722686434 17.385707430878437 20.550325131955592 35.94917021030163 10-11 24.350976018216357 27.276548423609775 27.65467687806557 20.71779868010829 12-13 25.616261561626157 24.597349305888777 28.22128828366729 21.565100848817778 14-15 22.56283402551417 26.12307030461472 26.727997749722853 24.586097920148255 16-17 21.788308486523157 29.228452768999126 27.22256233764085 21.760676406836872 18-19 21.426751339420154 27.763805376212424 30.038420427356932 20.771022857010486 20-21 22.230237968383395 26.800150901936902 30.298196788039032 20.671414341640663 22-23 21.96667223728864 26.658569960669205 30.29662734690028 21.078130455141878 24-25 21.87808203763644 26.925011748975685 30.172693394758966 21.02421281862891 26-27 21.927060925968824 26.973557930966013 30.154052354634807 20.945328788430352 28-29 21.43870012326988 27.139559703159517 30.357813572923646 21.06392660064696 30-31 21.77783513939701 27.058689209783026 30.17359060019558 20.989885050624387 32-33 21.860640588002667 27.142008534775318 30.021461038370084 20.975889838851923 34-35 21.870046494696957 26.98131939043963 30.051954928272416 21.096679186590997 36-37 22.150128742296975 26.83730812764352 29.960549918253605 21.0520132118059 38-39 21.87549642573471 27.042129997352397 29.927852793222133 21.15452078369076 40-41 21.915249189224966 27.14772652061685 29.886987888013767 21.050036402144418 42-43 21.91521749921406 27.360226351407253 29.55821764812946 21.166338501249236 44-45 22.039644588579844 27.33292519483098 29.4301504045535 21.197279812035674 46-47 22.305706769032216 27.590217912867942 28.89786058209375 21.20621473600609 48-49 22.413813073441936 27.712354493633708 28.51916506027087 21.35466737265349 50-51 22.26509820014661 27.97789938228807 28.415079915343846 21.34192250222148 52-53 22.237205685259028 28.213677052136937 28.137233813723384 21.41188344888065 54-55 22.1905808300598 28.088400276664537 28.04703297139699 21.67398592187867 56-57 22.184477104175425 28.24921485374272 28.246567363728726 21.31974067835313 58-59 22.11741151943346 28.04705726624419 28.241805516488245 21.593725697834106 60-61 22.17594683901627 28.04122756071369 28.50271281850177 21.28011278176827 62-63 22.028017155792064 27.908608342268643 28.70136875686695 21.362005745072345 64-65 22.053377897645483 28.086437116336022 28.379469530254646 21.480715455763853 66-67 22.00851165222686 28.114927161172037 28.247797626533057 21.62876356006804 68-69 21.924923101360598 27.99309029116414 28.403602446857622 21.67838416061764 70-71 22.05071099300755 28.026229139296387 28.297422422836494 21.62563744485957 72-73 22.000264738488013 27.975743336035876 28.230223207637707 21.79376871783841 74-75 21.92944719294472 28.158081969654354 28.000066184622003 21.91240465277893 76-77 22.017472740208813 28.243294670483316 27.813094627463308 21.926137961844567 78-79 22.21543807396376 27.978654753040455 27.77744684371639 22.028460329279394 80-81 22.16024542151211 28.154308444803327 27.546380562192645 22.139065571491923 82-83 22.074842203164874 28.29586037752086 27.742785366515626 21.88651205279864 84-85 21.95756820577484 28.066012367803992 27.67829809485513 22.29812133156603 86-87 21.867429607762674 28.13918931441204 27.617121827881547 22.37625924994374 88-89 21.745950328441186 28.04126611181892 27.74078792792495 22.47199563181495 90-91 21.759604740859384 28.211521883371415 27.738461818476644 22.290411557292554 92-93 21.586315644912297 28.04395989867118 27.736366358962822 22.63335809745371 94-95 21.514304151430416 28.507867696940615 27.621159223654384 22.356668927974585 96-97 21.493220905489792 28.847802130525285 27.20457080075055 22.454406163234374 98-99 21.51404640326696 28.752684684803942 26.86471836043114 22.86855055149796 100-101 21.199345426506916 29.03619182511173 26.625719972930177 23.138742775451178 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 47.0 1 37.0 2 34.0 3 44.5 4 39.5 5 44.5 6 70.0 7 133.5 8 250.5 9 330.0 10 333.0 11 329.0 12 351.0 13 393.5 14 452.5 15 493.5 16 515.5 17 525.5 18 535.5 19 593.5 20 679.5 21 754.5 22 863.0 23 1074.5 24 1306.0 25 1598.0 26 1981.5 27 2518.0 28 2975.5 29 3436.0 30 4122.5 31 4728.5 32 5289.5 33 5813.5 34 6466.0 35 7043.5 36 7626.5 37 8334.0 38 8876.0 39 9173.0 40 9470.5 41 10146.0 42 10557.0 43 10970.0 44 11495.0 45 11677.5 46 11932.5 47 11985.5 48 12109.0 49 11741.5 50 10962.0 51 10391.0 52 9754.0 53 9301.5 54 8840.5 55 8257.5 56 7374.0 57 6469.0 58 5899.5 59 5203.0 60 4245.5 61 3393.5 62 2639.0 63 2011.5 64 1479.5 65 1045.0 66 780.0 67 603.0 68 410.5 69 262.5 70 185.5 71 126.0 72 90.5 73 56.5 74 40.0 75 34.0 76 26.0 77 11.5 78 5.0 79 5.0 80 4.0 81 2.0 82 1.5 83 1.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02548107947118487 2 0.002978307990138491 3 3.309231100153879E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.01290600129060013 12-13 0.0 14-15 0.0 16-17 0.0 18-19 6.618462200307758E-4 20-21 6.618462200307758E-4 22-23 0.0014891539950692455 24-25 0.010258616410477026 26-27 0.004963846650230819 28-29 8.273077750384698E-4 30-31 0.002812846435130797 32-33 0.003143769545146185 34-35 0.0 36-37 0.010258616410477026 38-39 0.008273077750384697 40-41 0.0016546155500769396 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0024819233251154094 50-51 0.00711484686533084 52-53 0.0 54-55 0.004632923540215431 56-57 0.003971077320184656 58-59 0.0 60-61 0.002812846435130797 62-63 0.004302000430200043 64-65 0.0 66-67 0.003640154210169267 68-69 4.96384665023082E-4 70-71 6.618462200307758E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0033092311001538793 80-81 0.003640154210169267 82-83 0.018531694160861725 84-85 0.009762231745453945 86-87 0.006949385310323147 88-89 0.0 90-91 0.0011582308850538578 92-93 1.6546155500769396E-4 94-95 0.0 96-97 0.001985538660092328 98-99 0.0026473848801231033 100-101 0.0011582308850538578 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 302185.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.86828676271085 #Duplication Level Percentage of deduplicated Percentage of total 1 89.02270998616814 70.21068619582752 2 7.100546512912762 11.200158771047406 3 1.7920791480364386 4.240146364464372 4 0.7350333579598343 2.3188328662293802 5 0.3901735546298404 1.538615989688624 6 0.22462076331901387 1.0629272864581794 7 0.1588803869624293 0.8771436743946337 8 0.11729510285092332 0.7400691046006627 9 0.07843258067358819 0.5567258937693605 >10 0.35791637782031366 4.7236748313328585 >50 0.016839417861660655 0.9157222173943356 >100 0.0042098544654086845 0.519949598418367 >500 4.2098544654086843E-4 0.23142073441737027 >1k 8.419708930817369E-4 0.8639264719569547 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1386 0.4586594304813276 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1216 0.4024025017787117 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 697 0.2306534076807254 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 349 0.11549216539537038 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.309231100153879E-4 2 0.0 0.0 0.0 0.0 3.309231100153879E-4 3 0.0 0.0 0.0 0.0 3.309231100153879E-4 4 0.0 0.0 0.0 0.0 3.309231100153879E-4 5 0.0 0.0 0.0 0.0 3.309231100153879E-4 6 0.0 0.0 0.0 0.0 3.309231100153879E-4 7 0.0 0.0 0.0 0.0 3.309231100153879E-4 8 0.0 0.0 0.0 0.0 3.309231100153879E-4 9 0.0 0.0 0.0 0.0 3.309231100153879E-4 10-11 0.0 0.0 0.0 0.0 3.309231100153879E-4 12-13 0.0 0.0 0.0 0.0 3.309231100153879E-4 14-15 0.0 0.0 0.0 0.0 3.309231100153879E-4 16-17 0.0 0.0 0.0 3.309231100153879E-4 3.309231100153879E-4 18-19 1.6546155500769396E-4 0.0 0.0 3.309231100153879E-4 3.309231100153879E-4 20-21 3.309231100153879E-4 0.0 0.0 4.963846650230818E-4 3.309231100153879E-4 22-23 3.309231100153879E-4 0.0 0.0 0.0018200771050846335 3.309231100153879E-4 24-25 3.309231100153879E-4 0.0 0.0 0.0026473848801231033 3.309231100153879E-4 26-27 3.309231100153879E-4 0.0 0.0 0.003640154210169267 3.309231100153879E-4 28-29 3.309231100153879E-4 0.0 0.0 0.008273077750384699 3.309231100153879E-4 30-31 3.309231100153879E-4 0.0 0.0 0.013898770620646293 3.309231100153879E-4 32-33 3.309231100153879E-4 0.0 0.0 0.019359001935900195 3.309231100153879E-4 34-35 3.309231100153879E-4 0.0 0.0 0.026142925691215645 3.309231100153879E-4 36-37 3.309231100153879E-4 0.0 0.0 0.0355742343266542 3.309231100153879E-4 38-39 3.309231100153879E-4 0.0 0.0 0.049142081837285106 3.309231100153879E-4 40-41 3.309231100153879E-4 0.0 0.0 0.06502639111802373 3.309231100153879E-4 42-43 3.309231100153879E-4 0.0 0.0 0.08951470125916243 3.309231100153879E-4 44-45 3.309231100153879E-4 0.0 0.0 0.11301024207025498 3.309231100153879E-4 46-47 3.309231100153879E-4 0.0 0.0 0.1485844763969092 3.309231100153879E-4 48-49 3.309231100153879E-4 0.0 0.0 0.1882952495987557 3.309231100153879E-4 50-51 3.309231100153879E-4 0.0 0.0 0.20401409732448667 6.618462200307758E-4 52-53 3.309231100153879E-4 0.0 0.0 0.2334662541158562 6.618462200307758E-4 54-55 3.309231100153879E-4 0.0 0.0 0.2850902592782567 6.618462200307758E-4 56-57 3.309231100153879E-4 0.0 0.0 0.3787414994126115 6.618462200307758E-4 58-59 3.309231100153879E-4 0.0 0.0 0.495226434138028 6.618462200307758E-4 60-61 3.309231100153879E-4 0.0 0.0 0.6290848321392524 6.618462200307758E-4 62-63 3.309231100153879E-4 0.0 0.0 0.7732018465509538 6.618462200307758E-4 64-65 3.309231100153879E-4 0.0 0.0 0.9194698611777553 6.618462200307758E-4 66-67 3.309231100153879E-4 0.0 0.0 1.112728957426742 6.618462200307758E-4 68-69 3.309231100153879E-4 0.0 0.0 1.341893211112398 6.618462200307758E-4 70-71 3.309231100153879E-4 0.0 0.0 1.4598672998328839 6.618462200307758E-4 72-73 3.309231100153879E-4 0.0 0.0 1.622019623740424 6.618462200307758E-4 74-75 3.309231100153879E-4 0.0 0.0 1.8015454109237719 6.618462200307758E-4 76-77 3.309231100153879E-4 0.0 0.0 2.01763820176382 6.618462200307758E-4 78-79 3.309231100153879E-4 0.0 0.0 2.22860168439863 6.618462200307758E-4 80-81 3.309231100153879E-4 0.0 0.0 2.590631566755464 6.618462200307758E-4 82-83 3.309231100153879E-4 0.0 0.0 2.9637473732978146 6.618462200307758E-4 84-85 3.309231100153879E-4 0.0 0.0 3.595810513427205 6.618462200307758E-4 86-87 3.309231100153879E-4 0.0 0.0 4.324834124791105 6.618462200307758E-4 88-89 3.309231100153879E-4 0.0 0.0 5.017952578718335 6.618462200307758E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTGCG 50 0.0016121351 38.00861 9 GGTATCA 1005 0.0 36.395256 1 TACGCGT 55 0.0025737667 34.55328 5 GTATCAA 1635 0.0 33.992897 1 CGCGTAT 70 2.0470363E-4 33.93626 7 ACGCGTA 75 3.0690347E-4 31.673841 6 CGCAATA 30 0.003954707 31.658115 18-19 ATACGCG 60 0.003951605 31.658115 4 TCAACGC 1910 0.0 28.591755 4 AGGCGTG 100 4.9335657E-5 28.506456 7 ATCAACG 1920 0.0 28.442837 3 CAACGCA 1990 0.0 27.694715 5 TATCAAC 1980 0.0 27.580933 2 AACGCAG 2025 0.0 27.216042 6 AAGGCGT 110 9.4394156E-5 25.91496 6 AAAGGCG 115 1.2767327E-4 24.788223 5 TTAGTAC 115 1.2810461E-4 24.775917 3 ACGCAGA 2260 0.0 23.965605 7 GAGTACT 1360 0.0 23.918173 12-13 CGCAGAG 2245 0.0 23.9141 8 >>END_MODULE