FastQCFastQC Report
Thu 26 May 2016
SRR936908_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936908_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318543
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11650.36572770395205667No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8360.26244494463855744No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5650.17737008818275712No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGTC250.005008199757.0280089
GTATCAA12100.039.2940181
GGTATCA7250.035.4133951
GTGTAGG1255.5715645E-934.232951
TATCAAC14700.032.3542332
TCAACGC14750.031.9221124
ATCAACG14850.031.707153
CAACGCA15200.030.9770475
AACGCAG15800.030.101726
GTATATG1754.8821676E-927.1690061
ACGCAGA17200.026.5288267
CGCAGAG17400.026.2238988
GTACATG10750.024.3257391
GCAGAGT18400.024.2782299
TATTGGG1201.6923038E-423.780362
GTTCTAG1601.8370629E-623.772881
CAACGAC500.00165718723.7392872-73
GTGTAGC1852.5062582E-723.1303711
TATAAGG1252.2315342E-422.8291442
TACATGG10900.022.6895182
Produced by FastQC (version 0.11.5)