FastQCFastQC Report
Thu 26 May 2016
SRR936908_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936908_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318543
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13980.4388732447424681No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12920.4055967326232251No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7450.23387737291354702No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3470.10893348778657827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9650.039.8856931
GTATCAA15300.034.163371
CGCAGAA600.00394539331.6688122
CCTAACC700.00838627527.1404343
GTAAGGT1405.788479E-727.1404343
TATCAAC19300.026.582112
TCAACGC19500.026.3053464
ATCAACG19550.026.238073
GTATAGG1109.431027E-525.9189851
CAACGCA19900.025.7765945
AACGCAG20400.025.1527126
GTCCTAA1000.001647363923.7590711
ACGCAGA21950.023.376557
CGCAGAG22400.022.9141318
GGTAAGG1050.002191898222.620582
TAAGGTG1302.9368224E-421.921124
AGAGTAC20500.021.21264810-11
GCAGAGT23900.020.879459
GCAGTGC2308.918323E-820.6633058
GAGTACT15150.020.54414612-13
Produced by FastQC (version 0.11.5)