##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936908_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 318543 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.39068194874789 33.0 31.0 34.0 31.0 34.0 2 32.52311932768888 34.0 31.0 34.0 31.0 34.0 3 32.64204832628562 34.0 31.0 34.0 31.0 34.0 4 36.243336064518765 37.0 35.0 37.0 35.0 37.0 5 36.111608165930505 37.0 35.0 37.0 35.0 37.0 6 36.197728407153825 37.0 36.0 37.0 35.0 37.0 7 36.17554615860339 37.0 36.0 37.0 35.0 37.0 8 36.14482503147142 37.0 36.0 37.0 35.0 37.0 9 37.88927397557002 39.0 38.0 39.0 35.0 39.0 10-11 37.80473750796595 39.0 37.5 39.0 35.0 39.0 12-13 37.71091187061087 39.0 37.0 39.0 35.0 39.0 14-15 38.99813996854428 40.0 38.0 41.0 36.0 41.0 16-17 38.98231321987926 40.0 38.0 41.0 36.0 41.0 18-19 39.07490040591066 40.0 38.5 41.0 36.0 41.0 20-21 39.071601008341105 40.0 38.5 41.0 36.0 41.0 22-23 38.981663386104856 40.0 38.0 41.0 35.0 41.0 24-25 38.90562969520599 40.0 38.0 41.0 35.0 41.0 26-27 38.72557237170492 40.0 38.0 41.0 35.0 41.0 28-29 38.568524500616874 40.0 38.0 41.0 34.0 41.0 30-31 38.5630323064704 40.0 38.0 41.0 35.0 41.0 32-33 38.435760635141875 40.0 38.0 41.0 34.5 41.0 34-35 38.295057496162215 40.0 38.0 41.0 34.0 41.0 36-37 38.16172227925272 40.0 38.0 41.0 34.0 41.0 38-39 37.982236307186156 40.0 38.0 41.0 34.0 41.0 40-41 37.561341796868874 40.0 37.0 41.0 32.5 41.0 42-43 37.42290365821883 40.0 37.0 41.0 33.0 41.0 44-45 37.225876883183744 40.0 37.0 41.0 32.5 41.0 46-47 36.84441974866816 40.0 36.5 41.0 31.5 41.0 48-49 36.45636852795384 39.5 36.0 41.0 30.5 41.0 50-51 36.17542529579994 39.0 36.0 40.5 30.0 41.0 52-53 35.93224462631419 39.0 35.5 40.0 29.5 41.0 54-55 35.58893932687266 39.0 35.0 40.0 28.0 41.0 56-57 35.36033282790706 38.0 35.0 40.0 28.0 41.0 58-59 35.0506164002976 38.0 34.5 40.0 27.5 41.0 60-61 34.65998624989405 38.0 34.0 40.0 26.0 41.0 62-63 34.287292453452125 37.5 34.0 40.0 26.0 41.0 64-65 33.97300992330706 37.0 34.0 39.5 26.0 41.0 66-67 33.653717080582524 36.5 33.0 39.0 25.5 40.0 68-69 33.20268221244855 36.0 33.0 39.0 23.5 40.0 70-71 32.76011244949662 35.5 32.0 38.5 22.5 40.0 72-73 32.347312607717015 35.0 32.0 38.0 22.0 40.0 74-75 31.880722539814094 35.0 31.0 37.0 20.5 39.0 76-77 30.430304542871763 33.5 29.5 35.5 19.5 38.0 78-79 31.078899865952163 34.0 31.0 36.0 19.5 38.5 80-81 30.946719909086056 34.0 31.0 36.0 18.5 37.5 82-83 30.596068662629534 34.0 31.0 35.5 17.5 37.0 84-85 30.175944848890104 34.0 30.5 35.0 12.5 36.5 86-87 29.79008014616551 34.0 30.0 35.0 7.5 36.0 88-89 29.419817732613808 34.0 30.0 35.0 4.5 36.0 90-91 29.000453627924642 34.0 29.0 35.0 2.0 35.0 92-93 28.53856936112236 34.0 29.0 35.0 2.0 35.0 94-95 28.01661471135765 33.0 29.0 35.0 2.0 35.0 96-97 27.316814370430365 33.0 27.0 34.5 2.0 35.0 98-99 26.539489174146034 32.5 25.5 34.0 2.0 35.0 100-101 23.466029703996007 29.5 12.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.03044429007636751 1101 2 0.16677513004042765 1101 3 0.1583367549010859 1101 4 0.08810544898133799 1101 5 0.004697404466767807 1101 6 0.02141033785229496 1101 7 -0.054090210858554144 1101 8 0.09110655180841576 1101 9 -0.07990602686714254 1101 10-11 0.048574657915217756 1101 12-13 0.008807713345625245 1101 14-15 0.05409806185534194 1101 16-17 0.27212831656002834 1101 18-19 0.059485455058755576 1101 20-21 0.04214564965035095 1101 22-23 0.1421464552519609 1101 24-25 -0.033425890988297624 1101 26-27 0.0613718323291792 1101 28-29 0.16420935782111457 1101 30-31 0.06289058575835327 1101 32-33 0.20814477752241345 1101 34-35 0.22435151592692648 1101 36-37 0.019016402227613582 1101 38-39 0.1710698986292627 1101 40-41 0.24528689804477466 1101 42-43 -0.029221084024179333 1101 44-45 -0.06589864271850132 1101 46-47 0.21743008146825105 1101 48-49 0.19403971649348506 1101 50-51 0.13427628907324873 1101 52-53 0.25632706879134304 1101 54-55 -0.0246078280481683 1101 56-57 0.18527453721990383 1101 58-59 0.08301072032170254 1101 60-61 0.1546524419339832 1101 62-63 0.15516195891601825 1101 64-65 0.36555494457039117 1101 66-67 0.27671127344685686 1101 68-69 -0.029902342968952667 1101 70-71 -0.2381842627188817 1101 72-73 -0.4204079545985806 1101 74-75 -0.46567005820959295 1101 76-77 -0.012677956698354365 1101 78-79 -0.05825591509997352 1101 80-81 -0.034872481786511145 1101 82-83 0.010552390867257344 1101 84-85 -0.08942138291365254 1101 86-87 -0.44154837762533816 1101 88-89 -0.3301571265289702 1101 90-91 -0.35978771985015356 1101 92-93 -0.3002339808746832 1101 94-95 -0.5430386741350368 1101 96-97 -0.8242808852356909 1101 98-99 -0.9103936767388312 1101 100-101 -0.23974788935218072 1102 1 -0.17809536367765588 1102 2 -0.06618997094108181 1102 3 -0.030223192156377365 1102 4 -0.2191526219648523 1102 5 -0.3438562437045647 1102 6 -0.0071260083153532605 1102 7 -0.046846491804821255 1102 8 0.036587300339341766 1102 9 -0.0378450153776555 1102 10-11 0.15449461422167587 1102 12-13 0.10800420669990984 1102 14-15 0.09594180163958299 1102 16-17 0.060765803703134225 1102 18-19 0.014297923663988854 1102 20-21 -0.012462304487371512 1102 22-23 0.08566378516209738 1102 24-25 0.08314536217110913 1102 26-27 -0.08849182469248262 1102 28-29 -0.05599352212530562 1102 30-31 -0.022896854741091488 1102 32-33 -0.06392524376622077 1102 34-35 0.08946963997512114 1102 36-37 -0.11507312966150351 1102 38-39 -0.0583510822428579 1102 40-41 0.31546366552020544 1102 42-43 0.06323300985314262 1102 44-45 -0.08439564078099693 1102 46-47 -0.04907359819750923 1102 48-49 0.1553494064128742 1102 50-51 0.09114685892311059 1102 52-53 0.014767775542310346 1102 54-55 0.18770051438797708 1102 56-57 0.16628020621742934 1102 58-59 0.22488117057245205 1102 60-61 0.31497404392411 1102 62-63 -0.03280298260001757 1102 64-65 0.3046122245678333 1102 66-67 0.19606559775179022 1102 68-69 -0.031189029994045825 1102 70-71 0.23537935204442562 1102 72-73 0.034133422704698546 1102 74-75 0.03820794273125472 1102 76-77 -0.09693652198750868 1102 78-79 -0.29121403946709634 1102 80-81 0.20267027489309797 1102 82-83 0.31613009440499695 1102 84-85 0.1538481775197731 1102 86-87 0.19300460079101 1102 88-89 -0.006038571924005254 1102 90-91 -0.05799034465181663 1102 92-93 -0.22258127275230777 1102 94-95 -0.02692967151182657 1102 96-97 -0.09183903033332186 1102 98-99 -0.3387229171250574 1102 100-101 -0.31498369891851397 1103 1 0.09612823880681276 1103 2 0.26600844492307374 1103 3 0.1145143997291953 1103 4 0.14881136458708966 1103 5 -0.020034373512530124 1103 6 0.01727424241118314 1103 7 0.05970658814275964 1103 8 0.057605591979893234 1103 9 -0.03767121630077952 1103 10-11 0.08570294518070654 1103 12-13 0.17155341178281702 1103 14-15 0.07399183298040413 1103 16-17 0.20551621683208054 1103 18-19 0.042528876994659015 1103 20-21 0.1274844161234796 1103 22-23 0.09208580351072015 1103 24-25 0.1375672665531269 1103 26-27 0.1742382453969853 1103 28-29 0.09814489031985119 1103 30-31 0.37189059753539055 1103 32-33 0.2501581797906738 1103 34-35 0.06466195862574864 1103 36-37 0.2600322894506988 1103 38-39 2.533966448297065E-4 1103 40-41 0.2059645487614148 1103 42-43 0.13255889736810644 1103 44-45 0.271506404919613 1103 46-47 0.3466854194103348 1103 48-49 0.2230512697669127 1103 50-51 0.144776695381033 1103 52-53 0.09710152675321382 1103 54-55 0.33287117938314026 1103 56-57 0.09878633192260367 1103 58-59 0.45621913854784424 1103 60-61 0.3199643567624477 1103 62-63 0.3637509520971136 1103 64-65 0.4002430297419224 1103 66-67 0.5175881916317806 1103 68-69 0.4750463680343344 1103 70-71 0.4951306199229393 1103 72-73 0.6056887819844263 1103 74-75 0.1848416540538338 1103 76-77 0.3819611359987043 1103 78-79 0.35946263726662053 1103 80-81 0.5011416977661973 1103 82-83 0.8267717264068217 1103 84-85 0.4984447357282171 1103 86-87 0.20645835883990316 1103 88-89 0.18752373931880228 1103 90-91 -0.008681267211507304 1103 92-93 0.03881396344348431 1103 94-95 0.4118517405344413 1103 96-97 0.3056893188603631 1103 98-99 0.21872622527621743 1103 100-101 0.687928147910366 1104 1 -0.07475481608578605 1104 2 0.07987282275583141 1104 3 0.04659383744678536 1104 4 -0.09884250767321134 1104 5 -0.5122066491360115 1104 6 -0.15760259409337607 1104 7 -0.0013299558348194296 1104 8 0.14211436122077004 1104 9 0.07896297169597943 1104 10-11 0.03520919641233888 1104 12-13 0.23121052461152658 1104 14-15 0.150819579252655 1104 16-17 0.05658363379251341 1104 18-19 0.1463540614662051 1104 20-21 0.1795042974486165 1104 22-23 -0.0038350215204800975 1104 24-25 4.673930389031966E-5 1104 26-27 0.04617886417038619 1104 28-29 -0.1142196298351692 1104 30-31 0.025916127959511925 1104 32-33 0.004720155661573244 1104 34-35 0.12656995275769134 1104 36-37 -0.08387084382862042 1104 38-39 0.012377063742114558 1104 40-41 0.12042668009201662 1104 42-43 -0.1411355695802854 1104 44-45 -0.08642094503988318 1104 46-47 -0.17638611394781378 1104 48-49 -0.16024228631900428 1104 50-51 0.08121119429718959 1104 52-53 -0.10406551579020373 1104 54-55 -0.15999957010778587 1104 56-57 0.025880111344989132 1104 58-59 -0.46757276441027074 1104 60-61 -0.11386236041037279 1104 62-63 -0.24292505674875997 1104 64-65 -0.22273216239312887 1104 66-67 -0.4786479319477337 1104 68-69 -0.11234387960639935 1104 70-71 0.032539478936321586 1104 72-73 0.16375845420531476 1104 74-75 -0.34357024846636364 1104 76-77 -0.11418959422397634 1104 78-79 0.31509312488458363 1104 80-81 0.37986123532832394 1104 82-83 0.36722409224072194 1104 84-85 0.1443294328007596 1104 86-87 0.31717631990929007 1104 88-89 0.09892703681611792 1104 90-91 0.20971114810674507 1104 92-93 0.17436168519251538 1104 94-95 0.28710737321163293 1104 96-97 0.580285889988648 1104 98-99 0.42950111525016155 1104 100-101 1.2533985777235976 1105 1 0.04712161629025502 1105 2 0.07283145968703053 1105 3 0.03495306550475874 1105 4 0.05403646939296891 1105 5 0.08310068937100112 1105 6 0.08719343555779346 1105 7 0.10357924140237174 1105 8 0.23595178555179785 1105 9 0.21007773564040377 1105 10-11 0.24565190927329894 1105 12-13 0.1835924177977617 1105 14-15 0.19134100364691164 1105 16-17 0.03300573624395753 1105 18-19 0.16305260261589893 1105 20-21 0.09780156783379823 1105 22-23 0.22804789112235824 1105 24-25 0.07955645180132365 1105 26-27 0.16696866644321773 1105 28-29 0.21167851933741133 1105 30-31 0.44355351751838157 1105 32-33 0.3975942330138338 1105 34-35 0.16645125323660181 1105 36-37 0.45218551868698853 1105 38-39 0.46895137343564386 1105 40-41 0.1698505685682008 1105 42-43 0.5401261857817374 1105 44-45 0.5627239758534515 1105 46-47 0.3356853586532935 1105 48-49 0.0316605412900941 1105 50-51 0.010524918237827308 1105 52-53 0.2897924557282394 1105 54-55 0.2086169719586266 1105 56-57 -0.029474801803630157 1105 58-59 -0.2505826112546998 1105 60-61 -0.2276661189651108 1105 62-63 0.07003626712232602 1105 64-65 0.3124430418933315 1105 66-67 -0.13092629892050667 1105 68-69 -0.23915558834212192 1105 70-71 -0.2679473313499159 1105 72-73 -0.02098051748698282 1105 74-75 -0.017245350016885652 1105 76-77 0.12972527719804816 1105 78-79 0.12555717520938714 1105 80-81 0.385946363906303 1105 82-83 0.43891401937723273 1105 84-85 0.10721805123948869 1105 86-87 0.4714896487117066 1105 88-89 0.24883062309059767 1105 90-91 0.42596239279213677 1105 92-93 0.3156473069029886 1105 94-95 0.5423548087645891 1105 96-97 0.010753417529784315 1105 98-99 0.0692456979051741 1105 100-101 0.7177689644848861 1106 1 -0.17112839836132565 1106 2 0.10383421418507055 1106 3 -0.011287337706868072 1106 4 0.0756036029568179 1106 5 -0.0880298269508657 1106 6 -0.029579699295396722 1106 7 0.10738865358077021 1106 8 0.22551985681710818 1106 9 0.10060109026737507 1106 10-11 0.09516547664129149 1106 12-13 0.41164434301607145 1106 14-15 0.32377668318743247 1106 16-17 0.2789472471154326 1106 18-19 -0.17293755151877832 1106 20-21 -0.11826536060786452 1106 22-23 -0.00946895820073479 1106 24-25 0.24640870521514557 1106 26-27 0.1682140511938499 1106 28-29 -8.280738759793849E-4 1106 30-31 0.16567155068956652 1106 32-33 -0.13021447588846513 1106 34-35 0.07925966910720206 1106 36-37 0.002396501515327998 1106 38-39 0.06588626179056689 1106 40-41 0.41908868613252537 1106 42-43 0.2371460533314078 1106 44-45 0.3377928383043596 1106 46-47 0.547371463089803 1106 48-49 0.8672990573775294 1106 50-51 1.1426089597311204 1106 52-53 0.8626694410180491 1106 54-55 0.9784991732128034 1106 56-57 1.0805879069814566 1106 58-59 1.0132338336846445 1106 60-61 0.8107210345248959 1106 62-63 1.1336827536721756 1106 64-65 0.8046190953423178 1106 66-67 0.9978025295387383 1106 68-69 1.024924585132652 1106 70-71 1.2855016518300673 1106 72-73 1.3202256010150393 1106 74-75 0.9092916012812466 1106 76-77 0.7115543811070921 1106 78-79 0.7950760171480837 1106 80-81 1.0740287644220388 1106 82-83 1.318287517589738 1106 84-85 1.304091402936141 1106 86-87 1.4310219701088833 1106 88-89 1.4338215391077078 1106 90-91 1.637531284546224 1106 92-93 1.4662069974615086 1106 94-95 2.1442419329514273 1106 96-97 1.9717362458718135 1106 98-99 1.3942032083078004 1106 100-101 1.6119112606813317 1107 1 0.21911674455394348 1107 2 0.26648297829858336 1107 3 -0.012660544908577265 1107 4 0.09304842037111882 1107 5 -0.09464156532946788 1107 6 0.19048946965994418 1107 7 0.10586286780109333 1107 8 -0.031575047058389316 1107 9 0.1015674212611728 1107 10-11 0.07299072330999223 1107 12-13 0.02078888578893867 1107 14-15 0.24133339155891065 1107 16-17 0.2984773050952896 1107 18-19 0.20301606459351973 1107 20-21 0.0818923039227002 1107 22-23 0.23508907251842004 1107 24-25 0.08762526507680235 1107 26-27 0.4000317706527099 1107 28-29 0.25316439891417986 1107 30-31 0.37378873103745036 1107 32-33 0.30621111662500766 1107 34-35 0.21497831420943214 1107 36-37 0.518315533149952 1107 38-39 0.5394393082761724 1107 40-41 0.5831869467367383 1107 42-43 0.16243340965324649 1107 44-45 0.4442418261997929 1107 46-47 0.15069259013689873 1107 48-49 -0.2775234428767632 1107 50-51 -0.7719216068440033 1107 52-53 -1.3201451251501979 1107 54-55 -1.3269885696414363 1107 56-57 -1.0881640002507638 1107 58-59 -0.6622823905041457 1107 60-61 -0.7913031746094745 1107 62-63 -1.341853814327017 1107 64-65 -1.1252710480815509 1107 66-67 -1.1065866475541313 1107 68-69 -1.7528192862944678 1107 70-71 -1.4498762344480411 1107 72-73 -1.0679851608606583 1107 74-75 -0.7659703252775998 1107 76-77 -0.8771446322181049 1107 78-79 -0.5630513351049906 1107 80-81 -0.7767239565626021 1107 82-83 -0.9603209657791965 1107 84-85 -1.1783874903605742 1107 86-87 -0.3549357147654959 1107 88-89 -0.22625881894335365 1107 90-91 0.06693826247319024 1107 92-93 -0.185608687616309 1107 94-95 -0.36982072598459226 1107 96-97 -0.4885424643306102 1107 98-99 -0.5299398086792841 1107 100-101 -0.23627305424085066 1108 1 0.15966990547347848 1108 2 0.3304197599077838 1108 3 0.2689486504937264 1108 4 0.09215043186537741 1108 5 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0.28550460811272416 2316 60-61 0.4700956902734319 2316 62-63 0.010330570658915406 2316 64-65 0.3550909493429799 2316 66-67 -0.22507045544271875 2316 68-69 0.2461910633025184 2316 70-71 -0.04307030131501932 2316 72-73 0.01422736358251342 2316 74-75 0.5376377254688123 2316 76-77 0.39504499910731283 2316 78-79 -0.1058805429077303 2316 80-81 -0.05883393891582145 2316 82-83 -0.13645489051807047 2316 84-85 0.13913528352219728 2316 86-87 0.05229946432545418 2316 88-89 -0.8784742050262437 2316 90-91 -0.5911434850347987 2316 92-93 0.21702402092568818 2316 94-95 -0.6838618382085606 2316 96-97 -0.15535221298325652 2316 98-99 -0.47337473022265897 2316 100-101 -0.8837190407911457 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 3.0 6 14.0 7 24.0 8 55.0 9 131.0 10 251.0 11 435.0 12 665.0 13 952.0 14 1208.0 15 1691.0 16 2356.0 17 3452.0 18 3281.0 19 1844.0 20 1554.0 21 1510.0 22 1608.0 23 1711.0 24 1947.0 25 2248.0 26 2634.0 27 3293.0 28 3971.0 29 5006.0 30 6388.0 31 8362.0 32 10913.0 33 14712.0 34 20712.0 35 30991.0 36 50386.0 37 77729.0 38 53425.0 39 3078.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.65519959055128 20.00364233523928 15.307855826323408 22.033302247886034 2 15.583314758596439 21.10173828143395 40.209020729406376 23.105926230563238 3 19.469646074928896 25.084290297668755 30.491426562274366 24.954637065127987 4 13.102155752912479 17.688035838175693 37.89001798815231 31.319790420759517 5 13.87961913611834 37.408292234231915 34.477410929273965 14.234677700375778 6 31.36091516686915 36.96361244792697 17.447565948710224 14.22790643649366 7 27.573757934589473 33.52524941765921 21.273803768419864 17.627188879331452 8 25.998687775276807 35.40589496551486 19.85854343055726 18.736873828651078 9 26.231705709137255 17.218451569965655 20.73227392306195 35.817568797835136 10-11 24.41915220237142 27.26664845876381 27.651054959612985 20.66314437925178 12-13 25.661207881748656 24.643808004822485 28.22252502291936 21.4724590905095 14-15 22.577313276331097 26.053801030058814 26.795185942726718 24.573699750883367 16-17 21.59159046031462 29.189465786408743 27.387668226895585 21.831275526381052 18-19 21.3521629049904 27.63297717879774 30.216784940109843 20.798074976102015 20-21 22.25521989320056 26.728898320781312 30.395930194229315 20.619951591788812 22-23 21.826329916649662 26.768486979452806 30.287566515453562 21.11761658844397 24-25 21.854383637976092 26.692356592919314 30.42356985331628 21.029689915788317 26-27 21.87616742480607 26.83891970628769 30.32212291590146 20.962789953004776 28-29 21.4108343640713 27.197512415945152 30.447476313955455 20.94417690602809 30-31 21.799254731700273 26.84362864668192 30.24709379895336 21.110022822664444 32-33 21.74729942268391 27.01220871279545 30.20235886520815 21.038132999312495 34-35 21.943348307763788 27.133071516247416 29.97083596249172 20.95274421349708 36-37 22.13899344808858 26.86048227342222 30.006310178223295 20.994214100265907 38-39 21.879361092578783 27.013225540598878 30.040579255131274 21.066834111691062 40-41 21.907158239729768 27.139121371750036 29.74380144533531 21.209918943184885 42-43 21.86690650869741 27.261468624330153 29.64450639317141 21.227118473801028 44-45 22.144855104084588 27.163549832202648 29.483359965341766 21.208235098371002 46-47 22.31586052096659 27.591451690119843 28.83979374808699 21.25289404082658 48-49 22.339527159598546 27.711957255378394 28.687335775703747 21.26117980931931 50-51 22.278242579308397 27.97102359238388 28.386676503366974 21.364057324940745 52-53 22.1548492820413 28.163162157580473 28.132867879274947 21.549120681103275 54-55 22.23624440028505 28.044879341250635 28.093224462631415 21.6256517958329 56-57 22.257748561418712 28.316428237318036 28.209378325689155 21.216444875574098 58-59 22.16294189481483 27.874258734299605 28.329299341062274 21.63350002982329 60-61 22.187654514774554 27.94945650041204 28.615783071066986 21.24710591374642 62-63 21.98776931214938 28.018823204402544 28.718414782305686 21.274992701142388 64-65 21.938482402689747 28.06528474962564 28.5829542636316 21.413278584053018 66-67 21.923413793428203 28.10845003657277 28.380155897319987 21.58798027267904 68-69 21.990472228980433 27.90600940219908 28.431214045221598 21.672304323598894 70-71 21.999679790042727 27.9673574202379 28.299653733742282 21.733309055977095 72-73 21.898034783700634 27.794468512588686 28.33976894581528 21.967727757895396 74-75 21.97942092911915 28.112800307665744 27.962577799405075 21.945200963810034 76-77 21.95496369406956 28.274204738449754 27.94536373425252 21.825467833228167 78-79 22.12811243782188 28.083713060034754 27.696635121750607 22.09153938039276 80-81 22.040906022886027 28.06928594973865 27.778580061845638 22.11122796552969 82-83 22.104271800466186 28.29716599825769 27.693321927215365 21.905240274060763 84-85 22.036212945303856 28.14783420844027 27.733747733747734 22.082205112508145 86-87 22.074499394894513 27.97062844639362 27.619808532379814 22.335063626332055 88-89 21.88605675500036 27.944098930388943 27.739249733931505 22.430594580679188 90-91 21.8806262998862 27.966095043754656 27.92324294627791 22.230035710081232 92-93 21.672741199775228 27.926841902035203 27.862329418634218 22.53808747955535 94-95 21.444043661295336 28.41829831451327 27.735501957349555 22.40215606684184 96-97 21.62111254298904 28.60562471648032 27.455849413182936 22.317413327347698 98-99 21.4990723212688 28.758197634811655 26.984934246257108 22.75779579766244 100-101 21.09509652182376 29.027812224318666 26.764282059090384 23.112809194767195 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 63.0 1 52.0 2 40.0 3 49.5 4 47.0 5 43.5 6 70.0 7 140.5 8 243.5 9 340.0 10 364.5 11 329.0 12 341.5 13 422.5 14 504.5 15 524.5 16 541.5 17 588.5 18 622.5 19 649.0 20 685.5 21 765.5 22 932.0 23 1131.0 24 1352.5 25 1694.5 26 2113.0 27 2599.5 28 3051.0 29 3633.5 30 4350.5 31 4916.0 32 5518.0 33 6122.5 34 6784.0 35 7399.5 36 8188.5 37 8985.0 38 9491.5 39 9858.0 40 10159.0 41 10803.0 42 11156.0 43 11579.0 44 12273.0 45 12329.5 46 12354.5 47 12497.5 48 12687.5 49 12354.5 50 11492.0 51 10824.0 52 10252.5 53 9852.5 54 9394.0 55 8559.0 56 7630.5 57 6846.5 58 6114.5 59 5382.0 60 4429.5 61 3557.5 62 2854.0 63 2151.0 64 1542.0 65 1099.0 66 804.0 67 634.0 68 453.5 69 293.0 70 220.0 71 132.0 72 84.5 73 64.0 74 44.5 75 34.0 76 31.5 77 19.0 78 11.0 79 8.5 80 6.5 81 5.5 82 2.0 83 1.5 84 2.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020719337734622957 2 0.003139293596154993 3 3.1392935961549933E-4 4 0.0 5 0.0012557174384619973 6 0.0 7 3.1392935961549933E-4 8 0.0 9 3.1392935961549933E-4 10-11 0.0 12-13 0.010987527586542476 14-15 0.005493763793271238 16-17 0.0 18-19 4.70894039423249E-4 20-21 6.278587192309987E-4 22-23 0.0025114348769239947 24-25 1.5696467980774967E-4 26-27 0.0 28-29 3.1392935961549933E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.003139293596154993 38-39 0.00957484546827273 40-41 3.1392935961549933E-4 42-43 0.0 44-45 0.0012557174384619973 46-47 1.5696467980774967E-4 48-49 0.0 50-51 0.0025114348769239947 52-53 3.1392935961549933E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0017266114778852462 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.5696467980774967E-4 70-71 6.278587192309987E-4 72-73 9.41788078846498E-4 74-75 0.0048659050740402394 76-77 0.0 78-79 7.848233990387483E-4 80-81 0.0025114348769239947 82-83 1.5696467980774967E-4 84-85 0.0032962582759627425 86-87 0.0010987527586542476 88-89 0.005022869753847989 90-91 0.0017266114778852462 92-93 0.0 94-95 0.0 96-97 7.848233990387483E-4 98-99 0.001883576157692996 100-101 0.004395011034616991 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 318543.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.74470692963578 #Duplication Level Percentage of deduplicated Percentage of total 1 88.98320791440679 70.06956628878811 2 7.200506639698561 11.340035701759195 3 1.7414437159673248 4.113884251449087 4 0.7290262006274689 2.296278180497436 5 0.37737884081183615 1.4858293110586858 6 0.22722731971406476 1.0735769218374407 7 0.1534531299381012 0.8458535221087767 8 0.10662443816834014 0.6716888108082413 9 0.08724601203528805 0.618314548364841 >10 0.3706291290921382 4.858264220743392 >50 0.01804395998801203 1.0122883310182214 >100 0.004009768886210543 0.5298252711927712 >500 4.009768886210543E-4 0.23523231647116488 >1k 8.019537772421086E-4 0.8493623239025953 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1398 0.4388732447424681 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1292 0.4055967326232251 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 745 0.23387737291354702 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 347 0.10893348778657827 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.1392935961549933E-4 0.0 10-11 0.0 0.0 0.0 3.1392935961549933E-4 0.0 12-13 0.0 0.0 0.0 3.1392935961549933E-4 0.0 14-15 0.0 0.0 0.0 3.1392935961549933E-4 0.0 16-17 0.0 0.0 0.0 6.278587192309987E-4 0.0 18-19 0.0 0.0 0.0 7.848233990387483E-4 0.0 20-21 0.0 0.0 0.0 9.41788078846498E-4 0.0 22-23 0.0 0.0 0.0 0.002825364236539494 0.0 24-25 0.0 0.0 0.0 0.0034532229557704924 0.0 26-27 0.0 0.0 0.0 0.0048659050740402394 0.0 28-29 0.0 0.0 0.0 0.00926091610865723 0.0 30-31 0.0 0.0 0.0 0.016952185419236963 0.0 32-33 0.0 0.0 0.0 0.025114348769239944 0.0 34-35 0.0 0.0 0.0 0.030451147882703436 0.0 36-37 0.0 0.0 0.0 0.04253742822790016 0.0 38-39 0.0 0.0 0.0 0.05540853197213563 0.0 40-41 0.0 0.0 0.0 0.07000624719425635 0.0 42-43 0.0 0.0 0.0 0.0954345253231118 0.0 44-45 0.0 0.0 0.0 0.11709565113658124 0.0 46-47 3.1392935961549933E-4 0.0 0.0 0.1599470087240969 0.0 48-49 3.1392935961549933E-4 0.0 0.0 0.21096052966161555 0.0 50-51 3.1392935961549933E-4 0.0 0.0 0.2297962912385455 0.0 52-53 3.1392935961549933E-4 0.0 0.0 0.25522456936740096 0.0 54-55 3.1392935961549933E-4 0.0 0.0 0.30592416094530406 0.0 56-57 3.1392935961549933E-4 0.0 0.0 0.3968067105539912 0.0 58-59 3.1392935961549933E-4 0.0 0.0 0.513588432330957 3.1392935961549933E-4 60-61 3.1392935961549933E-4 0.0 0.0 0.6440260812511969 3.1392935961549933E-4 62-63 3.1392935961549933E-4 0.0 0.0 0.7779169531272073 3.1392935961549933E-4 64-65 3.1392935961549933E-4 0.0 0.0 0.9350385976147647 3.1392935961549933E-4 66-67 3.1392935961549933E-4 0.0 0.0 1.1343837409706068 3.1392935961549933E-4 68-69 3.1392935961549933E-4 0.0 0.0 1.3714004074803088 3.1392935961549933E-4 70-71 3.1392935961549933E-4 0.0 0.0 1.4911644581736216 3.1392935961549933E-4 72-73 3.1392935961549933E-4 0.0 0.0 1.6410657273900227 3.1392935961549933E-4 74-75 3.1392935961549933E-4 0.0 0.0 1.8174940274939333 3.1392935961549933E-4 76-77 3.1392935961549933E-4 0.0 0.0 2.0173100648891986 3.1392935961549933E-4 78-79 3.1392935961549933E-4 0.0 0.0 2.22089325459985 3.1392935961549933E-4 80-81 3.1392935961549933E-4 0.0 0.0 2.551774799634586 4.70894039423249E-4 82-83 3.1392935961549933E-4 0.0 0.0 2.9132644572318336 6.278587192309987E-4 84-85 3.1392935961549933E-4 0.0 0.0 3.5171075804522465 6.278587192309987E-4 86-87 3.1392935961549933E-4 0.0 0.0 4.252487105351554 6.278587192309987E-4 88-89 3.1392935961549933E-4 0.0 0.0 4.978448749462396 6.278587192309987E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 965 0.0 39.885693 1 GTATCAA 1530 0.0 34.16337 1 CGCAGAA 60 0.003945393 31.668812 2 CCTAACC 70 0.008386275 27.140434 3 GTAAGGT 140 5.788479E-7 27.140434 3 TATCAAC 1930 0.0 26.58211 2 TCAACGC 1950 0.0 26.305346 4 ATCAACG 1955 0.0 26.23807 3 GTATAGG 110 9.431027E-5 25.918985 1 CAACGCA 1990 0.0 25.776594 5 AACGCAG 2040 0.0 25.152712 6 GTCCTAA 100 0.0016473639 23.759071 1 ACGCAGA 2195 0.0 23.37655 7 CGCAGAG 2240 0.0 22.914131 8 GGTAAGG 105 0.0021918982 22.62058 2 TAAGGTG 130 2.9368224E-4 21.92112 4 AGAGTAC 2050 0.0 21.212648 10-11 GCAGAGT 2390 0.0 20.87945 9 GCAGTGC 230 8.918323E-8 20.663305 8 GAGTACT 1515 0.0 20.544146 12-13 >>END_MODULE