##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936907_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 659877 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.894481850405455 33.0 31.0 34.0 30.0 34.0 2 31.983116550508655 33.0 31.0 34.0 30.0 34.0 3 31.975124151925282 33.0 31.0 34.0 30.0 34.0 4 35.556523412696606 37.0 35.0 37.0 33.0 37.0 5 35.59205276134795 37.0 35.0 37.0 33.0 37.0 6 35.68078444922311 37.0 35.0 37.0 33.0 37.0 7 35.62718355087387 37.0 35.0 37.0 33.0 37.0 8 35.603065419767624 37.0 35.0 37.0 33.0 37.0 9 37.22452820752959 39.0 37.0 39.0 34.0 39.0 10-11 37.10792162781851 39.0 37.0 39.0 33.5 39.0 12-13 37.01584234637667 39.0 37.0 39.0 33.0 39.0 14-15 38.10792314325246 40.0 38.0 41.0 33.0 41.0 16-17 38.07498670206721 40.0 38.0 41.0 33.0 41.0 18-19 38.1051347448085 40.0 38.0 41.0 33.0 41.0 20-21 38.06296855323038 40.0 38.0 41.0 33.0 41.0 22-23 37.925924831445855 40.0 38.0 41.0 33.0 41.0 24-25 37.73570832139929 40.0 37.0 41.0 32.5 41.0 26-27 37.65521907870709 40.0 37.0 41.0 32.5 41.0 28-29 37.717101065804684 40.0 37.0 41.0 33.0 41.0 30-31 37.53522095784517 40.0 37.0 41.0 33.0 41.0 32-33 37.28208741932208 40.0 36.0 41.0 32.0 41.0 34-35 37.04446434714348 39.0 36.0 41.0 31.0 41.0 36-37 36.8416454884774 39.0 35.0 41.0 31.0 41.0 38-39 36.63072587770145 39.0 35.0 41.0 30.5 41.0 40-41 36.399582043320194 39.0 35.0 41.0 30.0 41.0 42-43 36.12831179143992 39.0 35.0 40.5 30.0 41.0 44-45 35.77219769744968 39.0 35.0 40.0 28.5 41.0 46-47 35.25358362240236 38.0 35.0 40.0 26.5 41.0 48-49 34.77628861136242 38.0 34.5 40.0 24.0 41.0 50-51 34.071810352535394 37.5 33.5 39.5 23.0 40.5 52-53 34.347763295280785 38.0 34.0 40.0 24.0 40.5 54-55 34.63190261215348 38.0 35.0 40.0 23.5 41.0 56-57 34.48267555923301 38.0 34.5 40.0 23.0 41.0 58-59 34.23463615188891 38.0 34.0 40.0 22.5 41.0 60-61 33.89332708974551 38.0 34.0 40.0 21.5 41.0 62-63 33.59150947828156 37.0 34.0 40.0 20.0 41.0 64-65 33.26522063960405 37.0 33.0 40.0 19.0 41.0 66-67 32.857484046269235 36.0 33.0 39.0 17.0 41.0 68-69 32.40391391122891 36.0 32.0 39.0 13.0 41.0 70-71 32.00310512413677 35.0 32.0 38.5 9.0 40.0 72-73 31.61824855238173 35.0 31.5 37.5 7.0 40.0 74-75 31.19151978323233 35.0 31.0 37.0 4.0 39.5 76-77 30.752489479099893 35.0 31.0 36.5 2.0 39.0 78-79 30.310695781183462 35.0 30.5 36.0 2.0 39.0 80-81 29.861536316616583 34.5 30.0 36.0 2.0 37.5 82-83 29.441929329253785 34.0 29.5 35.0 2.0 37.0 84-85 29.083908819370883 34.0 29.0 35.0 2.0 37.0 86-87 28.778567066286595 34.0 29.0 35.0 2.0 36.0 88-89 28.374633454416504 34.0 29.0 35.0 2.0 36.0 90-91 27.987097595460973 34.0 28.0 35.0 2.0 35.5 92-93 27.61958213424623 34.0 27.0 35.0 2.0 35.0 94-95 27.21954849161283 34.0 26.5 35.0 2.0 35.0 96-97 26.756600093653816 34.0 25.5 35.0 2.0 35.0 98-99 26.156470827138996 33.0 24.5 35.0 2.0 35.0 100-101 24.061985794322275 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4585871267703965 1101 2 0.310928082701043 1101 3 0.48218633876584605 1101 4 0.16220435509974607 1101 5 0.3256692357684017 1101 6 0.2836603741476509 1101 7 0.29974070288844246 1101 8 0.2365188707836836 1101 9 0.4193240863283094 1101 10-11 0.3967394618882807 1101 12-13 0.34975946326247254 1101 14-15 0.6518638262563172 1101 16-17 0.596394979114784 1101 18-19 0.6815996713757215 1101 20-21 0.6370762611125471 1101 22-23 0.7783076619728462 1101 24-25 0.7799717754511377 1101 26-27 0.6995129325544127 1101 28-29 0.674697348772888 1101 30-31 0.7697178954013495 1101 32-33 0.8320311802570757 1101 34-35 0.7573670014900316 1101 36-37 0.8490536567209332 1101 38-39 0.8356456485065706 1101 40-41 0.7911499456820721 1101 42-43 0.8335815783434697 1101 44-45 1.02975815563736 1101 46-47 1.0934771796485805 1101 48-49 0.985311608593797 1101 50-51 0.9652733037017427 1101 52-53 0.9489139684849377 1101 54-55 1.02680119539653 1101 56-57 1.008725116588998 1101 58-59 0.9018491065478358 1101 60-61 0.965373037768785 1101 62-63 0.9768175356214641 1101 64-65 1.0361532372037487 1101 66-67 1.1538958322641761 1101 68-69 0.9673140166222041 1101 70-71 0.8493609668186579 1101 72-73 1.1937104138593888 1101 74-75 1.0560306311468395 1101 76-77 1.0153395867751769 1101 78-79 1.1517252877281479 1101 80-81 1.080213100859968 1101 82-83 0.7321925152073021 1101 84-85 0.74073771737865 1101 86-87 1.0364451394736491 1101 88-89 0.9131882411404426 1101 90-91 1.1351292487657823 1101 92-93 0.8133274270784838 1101 94-95 1.0448593651410825 1101 96-97 1.057096519896323 1101 98-99 1.1466601802793477 1101 100-101 0.9670057780231218 1102 1 0.3365213990708611 1102 2 0.333228552184611 1102 3 0.14415816975175844 1102 4 0.2079790029870736 1102 5 0.18717158318624172 1102 6 0.2191063835373228 1102 7 0.15889563246590654 1102 8 0.20834985669917927 1102 9 0.19279826473206896 1102 10-11 0.24474415672395367 1102 12-13 0.30105054307467327 1102 14-15 0.5350797886976366 1102 16-17 0.47843723263591187 1102 18-19 0.48735084508464865 1102 20-21 0.44106687144118695 1102 22-23 0.4033076619728462 1102 24-25 0.5645961885966813 1102 26-27 0.569231242413565 1102 28-29 0.5702372548761758 1102 30-31 0.5965958296736531 1102 32-33 0.5421250769706916 1102 34-35 0.5232120719125675 1102 36-37 0.7721757224486296 1102 38-39 0.5046597330136109 1102 40-41 0.5434973635224551 1102 42-43 0.5829947238833739 1102 44-45 0.4616830382664645 1102 46-47 0.5852612172072682 1102 48-49 0.4465792142276044 1102 50-51 0.39895874971113443 1102 52-53 0.5392895553394013 1102 54-55 0.6253927446923058 1102 56-57 0.6748049287955737 1102 58-59 0.5321307966886764 1102 60-61 0.786969281900241 1102 62-63 0.5073339675463444 1102 64-65 0.7145569930722928 1102 66-67 0.5265484144238073 1102 68-69 0.4508820917395724 1102 70-71 0.6950182438139691 1102 72-73 0.831621211981453 1102 74-75 0.8042700677665522 1102 76-77 0.7424522628315131 1102 78-79 0.9815374943009161 1102 80-81 0.8665980773857846 1102 82-83 1.035009416615754 1102 84-85 1.1174982807589302 1102 86-87 1.2189568765628565 1102 88-89 1.0962868326897386 1102 90-91 1.0764438027563905 1102 92-93 1.0603931547780157 1102 94-95 1.0395776750002383 1102 96-97 0.6950073180183871 1102 98-99 0.7734207436596314 1102 100-101 1.2686489705113857 1103 1 0.24217243064255456 1103 2 0.4134930135706689 1103 3 0.29084486484586947 1103 4 0.15932930664387612 1103 5 0.21730036812830633 1103 6 0.2408899903771342 1103 7 0.22814984274285877 1103 8 0.16217140987141931 1103 9 0.30512436198839055 1103 10-11 0.3658434589593895 1103 12-13 0.18163912041025299 1103 14-15 0.3566329609421146 1103 16-17 0.6205609997031445 1103 18-19 0.5437507890911917 1103 20-21 0.3369325086897561 1103 22-23 0.3843156518078814 1103 24-25 0.6218979502368569 1103 26-27 0.5508395466733731 1103 28-29 0.6727133499788991 1103 30-31 0.7856194976755475 1103 32-33 0.809606879098439 1103 34-35 0.5822340170389779 1103 36-37 0.837755362367659 1103 38-39 0.8425829143031862 1103 40-41 1.1041910362622573 1103 42-43 1.0799420038937058 1103 44-45 1.2704492440639399 1103 46-47 1.3953411155576205 1103 48-49 1.4126574112384134 1103 50-51 1.2995757544921176 1103 52-53 1.3858217300389768 1103 54-55 1.3278144615377272 1103 56-57 1.3416223470666608 1103 58-59 1.3662098120653425 1103 60-61 1.4996124959244455 1103 62-63 1.4549827809158558 1103 64-65 1.5581387435137728 1103 66-67 1.5712920290165613 1103 68-69 1.6431630711942873 1103 70-71 1.6320094571490245 1103 72-73 1.5429103535587423 1103 74-75 1.8034947738009208 1103 76-77 1.6550863240797398 1103 78-79 1.6382841669976465 1103 80-81 1.7800128165855114 1103 82-83 1.6454968826966443 1103 84-85 1.6354323376999638 1103 86-87 1.629835758416668 1103 88-89 1.7562262090088119 1103 90-91 1.545703935417805 1103 92-93 1.6440091473696512 1103 94-95 1.4183060852168907 1103 96-97 1.049583167611356 1103 98-99 1.261777874210523 1103 100-101 1.7460200778896002 1104 1 0.313746070253174 1104 2 0.41189392866742836 1104 3 0.49771334303062176 1104 4 0.46611432893768523 1104 5 0.2557484387214899 1104 6 0.2590752040942519 1104 7 0.33893899697868335 1104 8 0.22321384981819392 1104 9 0.2902161051076533 1104 10-11 0.3023454899139395 1104 12-13 0.3833893596893816 1104 14-15 0.46332227158477934 1104 16-17 0.189028387708845 1104 18-19 0.24498891267593592 1104 20-21 0.324027484995284 1104 22-23 0.637596985701478 1104 24-25 0.25786393758424 1104 26-27 0.46531768473159474 1104 28-29 0.22731713875963067 1104 30-31 0.47975803445449827 1104 32-33 0.4863291052113823 1104 34-35 0.610881856523541 1104 36-37 0.700086699762899 1104 38-39 0.666291098816572 1104 40-41 0.5252376781839487 1104 42-43 0.42025236643077335 1104 44-45 0.3254163285936684 1104 46-47 0.09100432726712171 1104 48-49 0.09583287870812995 1104 50-51 0.09817567979033726 1104 52-53 0.2634231610448765 1104 54-55 0.16669206734578523 1104 56-57 0.25669576490638235 1104 58-59 0.39784864064810677 1104 60-61 0.38639407493324995 1104 62-63 0.416107217734492 1104 64-65 0.33969586702849597 1104 66-67 0.13347688089694287 1104 68-69 0.3809612946926606 1104 70-71 0.39223718084583226 1104 72-73 0.4892476320796497 1104 74-75 0.27829167834605784 1104 76-77 0.35626170984437877 1104 78-79 0.4192547648453022 1104 80-81 0.3445977548398247 1104 82-83 0.2973647189129984 1104 84-85 0.3213697282735346 1104 86-87 0.5979707818776951 1104 88-89 0.7898726478350646 1104 90-91 0.722127062620924 1104 92-93 0.6446000500939313 1104 94-95 0.9155587523037525 1104 96-97 0.7108120701955727 1104 98-99 0.6293412539990229 1104 100-101 0.920769710216792 1105 1 0.357369652432844 1105 2 0.35903025505721686 1105 3 0.3306585278324441 1105 4 0.2998465927061744 1105 5 0.2793176801067432 1105 6 0.2680241841261619 1105 7 0.3119950197500643 1105 8 0.38790742811637813 1105 9 0.517617235051155 1105 10-11 0.6009449206294235 1105 12-13 0.31674641321075114 1105 14-15 0.4867190025115917 1105 16-17 0.5902877140555063 1105 18-19 0.5679985732616188 1105 20-21 0.4166278645182899 1105 22-23 0.5152277701927872 1105 24-25 0.42185130119316483 1105 26-27 0.5078900313051093 1105 28-29 0.40570912564883344 1105 30-31 0.43082436472577257 1105 32-33 0.41012261019262297 1105 34-35 0.4087157720796668 1105 36-37 0.544724859076311 1105 38-39 0.6532234315892467 1105 40-41 0.8078205910230523 1105 42-43 0.6294159063453719 1105 44-45 0.6171099500194615 1105 46-47 0.7489538248304157 1105 48-49 0.6065716547464248 1105 50-51 0.688317785280482 1105 52-53 0.6893353100939166 1105 54-55 0.7665163223162494 1105 56-57 0.8390048173926843 1105 58-59 0.7245295394275999 1105 60-61 0.7137218879319178 1105 62-63 0.8227035863893519 1105 64-65 0.9568682049764803 1105 66-67 1.1816072988035273 1105 68-69 1.0182311010496718 1105 70-71 1.148965088725241 1105 72-73 0.914308010740104 1105 74-75 0.8915124486052548 1105 76-77 0.7526476157399244 1105 78-79 0.7997319719009788 1105 80-81 0.9327236443468756 1105 82-83 1.0031103953696316 1105 84-85 0.8580389825970762 1105 86-87 1.0089823400784113 1105 88-89 0.9648413803144713 1105 90-91 0.8161290896188085 1105 92-93 0.685711448722472 1105 94-95 0.5997352305027448 1105 96-97 0.6820567331532637 1105 98-99 0.7876842910456432 1105 100-101 0.8878202126535051 1106 1 0.5355189882092972 1106 2 0.3066329424904133 1106 3 0.4198782560352292 1106 4 0.29450990008134426 1106 5 0.38637219224600017 1106 6 0.37472749760913615 1106 7 0.38558640774823516 1106 8 0.3931677732072316 1106 9 0.5505764654680121 1106 10-11 0.6285025630175696 1106 12-13 0.6451629653561142 1106 14-15 0.648041341800031 1106 16-17 0.6502587019925983 1106 18-19 0.6279199353016196 1106 20-21 0.6167996464697438 1106 22-23 0.584265027789364 1106 24-25 0.5242142573696782 1106 26-27 0.6781641189520684 1106 28-29 0.5600418482018981 1106 30-31 0.6754151419436667 1106 32-33 0.7518541722631653 1106 34-35 0.6409382487936668 1106 36-37 0.7082498247199212 1106 38-39 0.6212185453470696 1106 40-41 0.40370545894180765 1106 42-43 0.5323285647665088 1106 44-45 0.8910067281535348 1106 46-47 0.7642042447418476 1106 48-49 0.9838048201151821 1106 50-51 0.6541484464501295 1106 52-53 0.6424617416101839 1106 54-55 0.47057419374699805 1106 56-57 0.7075038248747418 1106 58-59 0.8241019932373419 1106 60-61 0.9413215214637489 1106 62-63 0.9002726809075909 1106 64-65 0.7327843753841776 1106 66-67 0.8935862268841461 1106 68-69 1.2210445076766305 1106 70-71 1.027564874952148 1106 72-73 0.96589985682348 1106 74-75 0.8441158995137954 1106 76-77 0.8123513873081052 1106 78-79 0.7634401716261294 1106 80-81 1.0133275533406128 1106 82-83 0.9988845593641358 1106 84-85 0.7730718120366866 1106 86-87 0.8789968461098638 1106 88-89 1.2624364329942672 1106 90-91 1.3756000012083653 1106 92-93 1.3790234047462953 1106 94-95 1.0911003244102098 1106 96-97 1.23266751342506 1106 98-99 1.421422266309044 1106 100-101 1.1525247476970222 1107 1 0.3498688329345683 1107 2 0.26996043380425405 1107 3 0.4100052430905414 1107 4 0.5460672594885381 1107 5 0.3939606680938681 1107 6 0.2523187407293932 1107 7 0.402269985523958 1107 8 0.37961121323429126 1107 9 0.5154720778038993 1107 10-11 0.47561230074164484 1107 12-13 0.510070876680409 1107 14-15 0.28705065828375353 1107 16-17 0.4523372310335816 1107 18-19 0.4117467655448337 1107 20-21 0.4770031947278923 1107 22-23 0.6426621621250703 1107 24-25 0.5445229619787213 1107 26-27 0.45950347878972764 1107 28-29 0.47275131534822634 1107 30-31 0.33871034841293124 1107 32-33 0.16107226407511632 1107 34-35 0.36917980092601255 1107 36-37 0.5400657703583889 1107 38-39 0.5871718706860634 1107 40-41 0.5629496861041261 1107 42-43 0.7420463029825441 1107 44-45 0.7465858567702028 1107 46-47 0.670785740913793 1107 48-49 0.5628104469023825 1107 50-51 0.22865879297412306 1107 52-53 0.03486711626811001 1107 54-55 0.2795632168998452 1107 56-57 0.3074300309442961 1107 58-59 0.5036072664286237 1107 60-61 0.19720658741707808 1107 62-63 0.2262727424025428 1107 64-65 0.3405404249491042 1107 66-67 0.31815499360309474 1107 68-69 0.3277628300946205 1107 70-71 0.4090979438572262 1107 72-73 0.667718569734344 1107 74-75 0.6538290256091699 1107 76-77 0.5833826574861298 1107 78-79 0.8607436184176294 1107 80-81 0.5274022082002539 1107 82-83 0.8016656681179413 1107 84-85 0.9935357913743879 1107 86-87 0.8453068421126915 1107 88-89 0.8471180429318501 1107 90-91 0.5756907062478724 1107 92-93 0.5161186911589866 1107 94-95 0.6590476232448808 1107 96-97 0.9211525855278815 1107 98-99 0.7738974377261911 1107 100-101 0.8896231088568136 1108 1 0.036095023342927135 1108 2 0.23643185659650712 1108 3 0.24504031291681727 1108 4 0.14135975494455977 1108 5 0.21046941133487707 1108 6 0.315532743589678 1108 7 0.3132706863507124 1108 8 0.036038022421386984 1108 9 0.20423947739480042 1108 10-11 0.1642814136545212 1108 12-13 0.11658229787727947 1108 14-15 0.3764174641661313 1108 16-17 0.4617613565670382 1108 18-19 0.422264864538036 1108 20-21 0.46420598436021976 1108 22-23 0.3381942491643315 1108 24-25 0.2579446488630097 1108 26-27 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2316 84-85 -1.661076152617433 2316 86-87 -1.4359783205752876 2316 88-89 -2.1463618317547493 2316 90-91 -2.213096218951602 2316 92-93 -2.2444283643963097 2316 94-95 -2.3773780034388423 2316 96-97 -2.7095417272793902 2316 98-99 -2.419741880053465 2316 100-101 -2.2885247833661886 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1668.0 3 480.0 4 454.0 5 428.0 6 547.0 7 596.0 8 767.0 9 972.0 10 1192.0 11 1636.0 12 2447.0 13 3256.0 14 3750.0 15 4885.0 16 6842.0 17 9400.0 18 7744.0 19 4661.0 20 4197.0 21 4230.0 22 4601.0 23 4958.0 24 5556.0 25 6219.0 26 7095.0 27 8432.0 28 9966.0 29 11904.0 30 14603.0 31 18343.0 32 23661.0 33 31162.0 34 43465.0 35 63172.0 36 91998.0 37 124372.0 38 109507.0 39 20695.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.81250028433173 19.57354789890225 14.897466187270734 20.716485629495285 2 14.745693035588866 21.885917208592538 40.081580891443416 23.286808864375182 3 19.131082302554553 27.539689280102884 30.92442505899553 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31.586303019456835 21.212711778293095 24-25 21.268452167478085 26.51781848731085 31.622971863311605 20.590757481899455 26-27 20.960359670504214 26.122207606606594 32.41682050971012 20.500612213179075 28-29 20.176231428671084 26.99164565062775 32.23036409434394 20.601758826357223 30-31 20.405228272579166 26.642022835976253 31.894858380102786 21.0578905113418 32-33 20.379653173581513 26.882079927462726 31.76045414640581 20.977812752549948 34-35 21.187653605959113 26.701245222200825 31.25533498796927 20.855766183870784 36-37 21.3649916871424 26.09047027347899 31.773083626665134 20.771454412713478 38-39 20.907145310186564 26.839547223365713 31.4737529196906 20.779554546757126 40-41 20.564261754126303 27.07573836719096 31.720445598306412 20.639554280376327 42-43 20.44053244208828 27.463484832861223 30.505859410540875 21.590123314509626 44-45 20.735622668343602 27.166957401144593 30.433948689723767 21.663471240788038 46-47 21.30573330483281 27.634668764790334 29.801390764920704 21.258207165456152 48-49 21.35361509579282 27.60241640633469 29.376059868900327 21.667908628972164 50-51 20.851425699965212 28.04082501949769 29.728047949540205 21.379701330996895 52-53 21.222687827094163 28.62380911952165 28.72485582083578 21.42864723254841 54-55 20.659822512929445 27.892958891107604 29.259894381092305 22.187324214870646 56-57 21.16796012945494 28.336273538381995 28.57280400039725 21.922962331765813 58-59 21.699804772645425 27.645785553175763 28.819120908281054 21.83528876589776 60-61 21.29584281639826 27.743290842126488 29.944549657396642 21.01631668407861 62-63 21.14896027063973 27.933225621309088 29.632154086321762 21.28566002172942 64-65 20.866604911787046 28.470793529988764 29.343144374519593 21.319457183704596 66-67 20.785465960487855 28.11519257526529 28.85508801328445 22.244253450962404 68-69 20.53038022283278 28.31792790989647 29.335353020736672 21.816338846534084 70-71 21.27623452228556 28.28307173655844 28.711903998569042 21.728789742586955 72-73 20.97905383795713 27.85078644736782 29.06884389129641 22.101315823378638 74-75 20.517900806927173 29.23477593862321 28.43391246742655 21.81341078702307 76-77 20.86895348211355 28.300762549777236 28.594365819212253 22.23591814889696 78-79 20.87199873227786 28.195975285483897 28.82839576283588 22.103630219402365 80-81 21.13707309159987 28.56509353438539 28.25035589913235 22.047477474882392 82-83 20.77060486218944 28.398179711762424 28.88562694099379 21.945588485054348 84-85 20.702591880432607 28.38557936686057 28.34319517654266 22.568633576164164 86-87 20.722957890011767 28.588478289880744 28.44099930847902 22.24756451162847 88-89 20.941265163041088 28.11228196847635 28.08854290702631 22.857909961456258 90-91 20.51511654532689 27.786556230030495 28.72954902849829 22.96877819614432 92-93 20.852129641636015 28.143239433308842 28.75336589464361 22.251265030411535 94-95 20.484621253141498 27.825529068629905 28.750271113072728 22.939578565155866 96-97 19.933423285467573 27.84708743939039 29.184082567867975 23.035406707274063 98-99 20.221800212893 28.2382914540076 28.18776161143158 23.35214672166782 100-101 20.181788663282056 27.82242034892013 28.6499600948014 23.34583089299641 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 712.0 1 541.0 2 425.0 3 467.5 4 435.0 5 481.5 6 790.5 7 1448.0 8 2174.5 9 2392.5 10 2005.0 11 1696.0 12 1751.0 13 1771.0 14 1750.0 15 1766.5 16 1811.0 17 1938.0 18 2044.5 19 2078.5 20 2195.5 21 2474.5 22 2825.5 23 3196.0 24 3543.5 25 4258.0 26 5265.5 27 6184.0 28 6753.5 29 7596.0 30 9053.0 31 10533.5 32 12155.0 33 13674.0 34 15339.0 35 16688.5 36 18092.5 37 19610.5 38 19840.0 39 19288.5 40 18598.0 41 18554.5 42 18831.0 43 19510.0 44 20269.0 45 20569.0 46 20750.0 47 23447.5 48 33279.0 49 32794.5 50 22371.0 51 18308.5 52 17143.5 53 17138.5 54 16963.0 55 16287.5 56 14908.5 57 13362.5 58 12399.5 59 11231.5 60 9734.5 61 8383.0 62 7057.5 63 5580.5 64 4209.5 65 3232.0 66 2337.0 67 1770.0 68 1239.0 69 752.5 70 543.5 71 420.0 72 326.5 73 261.0 74 192.0 75 136.5 76 106.0 77 60.0 78 35.5 79 23.5 80 13.0 81 13.0 82 12.5 83 6.0 84 3.5 85 4.5 86 4.5 87 3.0 88 4.0 89 3.0 90 0.0 91 1.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06607291965017723 2 0.09910937947526584 3 0.07652941381499885 4 0.13926837880392862 5 0.10956587364008748 6 0.10971741703378053 7 0.11593069617519629 8 0.10365568128605787 9 0.12290169228507737 10-11 0.11267251321079533 12-13 0.0951692512392461 14-15 0.09895783608157278 16-17 0.11630955465942897 18-19 0.0924414701527709 20-21 0.07365008933483058 22-23 0.07880256472039486 24-25 0.0659971479533307 26-27 0.05826843487498428 28-29 0.06970996109881084 30-31 0.052661329308340804 32-33 0.053646361367345734 34-35 0.04788771240700919 36-37 0.0540252198515784 38-39 0.05250978591464772 40-41 0.0680429837681871 42-43 0.06440594231955349 44-45 0.051600525552489326 46-47 0.03637041448633609 48-49 0.04584187659215278 50-51 0.027808212742677803 52-53 0.042583693627751835 54-55 0.050918580280870526 56-57 0.05190361233987546 58-59 0.05902615184344961 60-61 0.055767968879048675 62-63 0.06099621596145948 64-65 0.042432150234058774 66-67 0.06963418940196431 68-69 0.055919512272741737 70-71 0.027050495774212466 72-73 0.02091298832964325 74-75 0.03243028625031635 76-77 0.04750885392277652 78-79 0.0646332574100931 80-81 0.042432150234058774 82-83 0.06379976874478123 84-85 0.06561828946909803 86-87 0.0698615044925039 88-89 0.09486616445185997 90-91 0.0657698328627911 92-93 0.07660518551184539 94-95 0.08456121368073141 96-97 0.09660891347933025 98-99 0.1304030902728842 100-101 0.12350786585984964 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 659877.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.13210675392228 #Duplication Level Percentage of deduplicated Percentage of total 1 75.5628072144972 37.881227179032216 2 13.141367999086082 13.176089268455227 3 4.563080075487312 6.86270452413077 4 2.132329257122306 4.275926318102689 5 1.1736683179112706 2.9419232703612104 6 0.725060296869141 2.180928012340461 7 0.4536486174328425 1.5919652642538755 8 0.342296624003652 1.3728040716846623 9 0.23688839840864703 1.0688143030009167 >10 1.4958900711208611 14.042537047783796 >50 0.11520743858521013 3.911858226293664 >100 0.048636370084651576 4.184940525793109 >500 0.004863637008463401 1.7032656127295667 >1k 0.003951705069376512 3.914447865633388 >5k 3.0397731302896254E-4 0.8905685104045439 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5844 0.8856195927422837 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3906 0.5919284957651199 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3710 0.5622259906012788 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2907 0.44053664546574595 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2611 0.395679800932598 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2092 0.31702877960589626 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1687 0.2556537051602041 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1529 0.23170984895669952 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1418 0.21488853225676904 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1269 0.19230856659650208 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1200 0.18185207243168044 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1195 0.18109435546321512 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1133 0.17169866505424494 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1030 0.15608969550385904 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 982 0.14881561260659185 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 974 0.1476032654570473 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 816 0.1236594092535427 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 809 0.12259860549769125 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 760 0.11517297920673095 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 747 0.11320291508872109 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 734 0.11123285097071121 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 709 0.10744426612838452 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 685 0.10380722467975093 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 668 0.10123098698696878 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 7.577169684653352E-5 0.0 20-21 0.0 0.0 0.0 1.5154339369306705E-4 0.0 22-23 0.0 0.0 0.0 5.304018779257347E-4 0.0 24-25 0.0 0.0 0.0 0.0015154339369306703 0.0 26-27 0.0 0.0 0.0 0.0027277810864752066 0.0 28-29 0.0 0.0 0.0 0.007122539503574152 0.0 30-31 0.0 0.0 0.0 0.014548165794534437 0.0 32-33 0.0 0.0 0.0 0.026217007108900597 0.0 34-35 0.0 0.0 0.0 0.036900816364261825 0.0 36-37 0.0 0.0 0.0 0.04531147471422704 0.0 38-39 0.0 0.0 0.0 0.05372213306419227 0.0 40-41 0.0 0.0 0.0 0.06917955922088512 0.0 42-43 0.0 0.0 0.0 0.08766785325143929 0.0 44-45 0.0 0.0 0.0 0.10433762655767666 0.0 46-47 0.0 0.0 0.0 0.1247959847062407 0.0 48-49 0.0 0.0 0.0 0.13941992219762167 0.0 50-51 0.0 0.0 0.0 0.1609390841020372 0.0 52-53 0.0 0.0 0.0 0.22201107176034324 0.0 54-55 0.0 0.0 0.0 0.3078604042874657 0.0 56-57 0.0 0.0 0.0 0.379767744594826 0.0 58-59 0.0 0.0 0.0 0.48918207484122045 0.0 60-61 0.0 0.0 0.0 0.6282231385546094 0.0 62-63 0.0 0.0 0.0 0.71323898241642 0.0 64-65 0.0 0.0 0.0 0.7687796362049291 0.0 66-67 0.0 0.0 0.0 0.9419179634992582 0.0 68-69 0.0 0.0 0.0 1.1326353244619831 0.0 70-71 0.0 0.0 0.0 1.2113621174855314 0.0 72-73 0.0 0.0 0.0 1.376620188307821 0.0 74-75 0.0 0.0 0.0 1.6107547315636097 0.0 76-77 0.0 0.0 0.0 1.8326142599302597 0.0 78-79 0.0 0.0 0.0 2.0250743699204548 0.0 80-81 0.0 0.0 0.0 2.2431453134447783 0.0 82-83 0.0 0.0 0.0 2.457503443823622 0.0 84-85 0.0 0.0 0.0 2.752255344556637 0.0 86-87 0.0 0.0 0.0 3.2784140074589656 0.0 88-89 0.0 0.0 0.0 3.8755707503064967 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCGT 45 9.609052E-4 42.23385 6 GTTCTAG 170 1.11422196E-7 25.163528 1 GAGTACT 3780 0.0 24.381323 12-13 GTACATG 4585 0.0 24.361635 1 TACATGG 4740 0.0 23.364449 2 CTAATAC 125 2.2479764E-4 22.809738 3 AGTACTT 3985 0.0 22.709835 12-13 GTACTTT 4145 0.0 22.405418 14-15 ACATGGG 4825 0.0 22.061235 3 TAGTACT 155 3.890337E-5 21.460775 4 CCAAGAC 360 0.0 21.12013 3 CATGGGA 3115 0.0 21.052328 4 TAAGGCG 115 0.003715685 20.657862 5 ACTTTTT 4685 0.0 20.22929 16-17 ATGGGAG 1010 0.0 20.228342 5 TACTTTT 4545 0.0 19.91095 14-15 CCTACGC 120 0.004749635 19.80012 3 GTCTACC 170 7.907412E-5 19.571632 1 GTATCAA 11135 0.0 19.379543 1 GCATAGA 150 7.6823815E-4 19.012442 1 >>END_MODULE