FastQCFastQC Report
Thu 26 May 2016
SRR936907_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936907_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659877
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78481.1893125537031901No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56810.8609180195703139No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT50910.7715074172914043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44200.6698218001233563No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20690.31354328155095573No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18180.27550588973399587No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16840.2551990749791249No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA16240.24610647135754085No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14210.2153431624378483No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14090.21352464171353144No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13850.20988760026489786No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT13350.2023104305802445No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC12100.18336750636861113No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC12030.18230670261275964No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11430.17321409899117562No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT11430.17321409899117562No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10470.15866593319664118No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG10410.1577566728344828No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT9560.1448754843705721No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA9560.1448754843705721No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC7170.10865661327792908No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6800.10304950771128558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG1753.6379788E-1232.5774041
TCGACGT350.00839381127.14166556-57
TAAGGTG2301.3460522E-1024.7834015
AGTACTT60500.024.18075812-13
AGGTAAG1401.7765102E-523.7525562
GTAAGGT2002.050001E-823.7507574
GAGTACT59550.023.56949412-13
GTACATG46800.023.4498161
ATGGGAG8500.022.9124975
TACATGG48500.022.3323022
ACATGGG46300.022.2648163
CCTTGCG753.0337931E-522.16653454-55
GTACTTT63250.021.96627214-15
GTACTAA1100.002873021221.594871
TACTTTT67500.021.46283514-15
TTAGACT1553.902218E-521.4522974
CATGGGG14300.020.927244
TTTAGGC2308.99563E-820.6543983
TAGGCAT2309.002906E-820.6528345
GGTGTGC4650.020.4292138
Produced by FastQC (version 0.11.5)