##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936907_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 659877 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.218248249294945 33.0 31.0 34.0 31.0 34.0 2 32.192873823454974 33.0 31.0 34.0 30.0 34.0 3 32.361144576943886 33.0 31.0 34.0 31.0 34.0 4 36.092626353093074 37.0 35.0 37.0 35.0 37.0 5 36.05006387554044 37.0 35.0 37.0 35.0 37.0 6 36.132273135751056 37.0 35.0 37.0 35.0 37.0 7 36.07018580735501 37.0 35.0 37.0 35.0 37.0 8 36.0671776709902 37.0 35.0 37.0 35.0 37.0 9 37.74644062454063 39.0 37.0 39.0 35.0 39.0 10-11 37.681542166191576 39.0 37.0 39.0 35.0 39.0 12-13 37.588930967437875 39.0 37.0 39.0 35.0 39.0 14-15 38.71418764406094 40.0 38.0 41.0 34.5 41.0 16-17 38.67341110540298 40.0 38.0 41.0 34.5 41.0 18-19 38.8179274319305 40.0 38.0 41.0 35.0 41.0 20-21 38.912621594630515 40.0 38.0 41.0 35.0 41.0 22-23 38.83727194613542 40.0 38.0 41.0 35.0 41.0 24-25 38.70992169752848 40.0 38.0 41.0 35.0 41.0 26-27 38.49911725973173 40.0 38.0 41.0 34.0 41.0 28-29 38.31619226007271 40.0 38.0 41.0 34.0 41.0 30-31 38.07820321059834 40.0 37.0 41.0 34.0 41.0 32-33 37.9297611524572 40.0 37.0 41.0 33.5 41.0 34-35 37.76644889880993 40.0 36.0 41.0 33.0 41.0 36-37 37.527408895900294 40.0 36.0 41.0 33.0 41.0 38-39 37.301646367429086 40.0 36.0 41.0 33.0 41.0 40-41 37.06278670115795 40.0 35.5 41.0 32.5 41.0 42-43 36.72944730608886 39.0 35.0 41.0 31.5 41.0 44-45 36.193523944613915 39.0 35.0 41.0 30.0 41.0 46-47 35.658505297199326 39.0 35.0 41.0 28.0 41.0 48-49 35.10180760959997 39.0 35.0 41.0 25.0 41.0 50-51 34.750677777828294 39.0 35.0 40.0 23.5 41.0 52-53 34.486008756177284 38.5 34.5 40.0 23.0 41.0 54-55 34.23876495165008 38.0 34.0 40.0 22.0 41.0 56-57 33.93940537403183 38.0 34.0 40.0 20.5 41.0 58-59 33.61657020929658 38.0 34.0 40.0 19.5 41.0 60-61 33.24462892326903 37.0 33.0 40.0 17.5 41.0 62-63 32.89104939253831 36.5 33.0 39.5 14.0 41.0 64-65 32.48819552734828 36.0 32.5 39.0 9.5 40.5 66-67 32.085402279515726 35.5 32.0 39.0 7.5 40.0 68-69 31.649075509526774 35.0 31.0 38.5 4.5 40.0 70-71 31.139978359603383 35.0 31.0 37.5 2.0 40.0 72-73 30.812742374715288 35.0 31.0 37.0 2.0 39.0 74-75 30.380798997388908 35.0 30.0 36.5 2.0 39.0 76-77 29.026332180088108 33.5 28.5 35.0 2.0 37.5 78-79 29.69686017242607 34.0 30.0 36.0 2.0 38.0 80-81 29.51038223790191 34.0 30.0 35.0 2.0 37.0 82-83 29.19739208973794 34.0 29.5 35.0 2.0 37.0 84-85 28.820091471592434 34.0 29.0 35.0 2.0 36.0 86-87 28.478768770543603 34.0 29.0 35.0 2.0 36.0 88-89 28.10217282917877 34.0 29.0 35.0 2.0 35.5 90-91 27.67967515158128 34.0 27.5 35.0 2.0 35.0 92-93 27.2730236089453 33.0 27.0 35.0 2.0 35.0 94-95 26.796868204225937 33.0 25.5 35.0 2.0 35.0 96-97 26.156466280837186 33.0 24.5 35.0 2.0 35.0 98-99 25.489416209384476 33.0 22.0 34.5 2.0 35.0 100-101 22.665305806991302 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29172437404429985 1101 2 0.21828826823627878 1101 3 0.1721293564437545 1101 4 0.19388215858844404 1101 5 0.23382671264664623 1101 6 0.28111756285206724 1101 7 0.344114744643079 1101 8 0.2964794829576789 1101 9 0.3036588070082189 1101 10-11 0.349831356991416 1101 12-13 0.3570600466877707 1101 14-15 0.39896218463832867 1101 16-17 0.531684296412962 1101 18-19 0.5293952978131173 1101 20-21 0.4095817342193655 1101 22-23 0.42661232768315926 1101 24-25 0.3709510667895728 1101 26-27 0.27379638073900026 1101 28-29 0.4648270219357684 1101 30-31 0.4932944143849909 1101 32-33 0.57901762483894 1101 34-35 0.6261949794570043 1101 36-37 0.8217777055122966 1101 38-39 0.7719503626604123 1101 40-41 0.5316119665597441 1101 42-43 0.8031867670838793 1101 44-45 0.9064380629750772 1101 46-47 1.0008608958268965 1101 48-49 0.9194046987632376 1101 50-51 0.9256043428138199 1101 52-53 0.7322345523235896 1101 54-55 1.09024688706684 1101 56-57 1.2092267354064319 1101 58-59 0.7376874334356458 1101 60-61 0.8639202422195069 1101 62-63 1.0549125360522353 1101 64-65 1.0430219323604533 1101 66-67 1.0814152248606987 1101 68-69 0.8832981160315931 1101 70-71 1.098990929856953 1101 72-73 1.158295563871036 1101 74-75 1.0440443669967898 1101 76-77 0.770647669212071 1101 78-79 0.8454683131108531 1101 80-81 0.9147401531397605 1101 82-83 0.9548340741831964 1101 84-85 0.9641014413027378 1101 86-87 0.8746012659318296 1101 88-89 0.8966021236596511 1101 90-91 1.1693036546466757 1101 92-93 1.1634273243593647 1101 94-95 1.2987073983628115 1101 96-97 1.2688236127107508 1101 98-99 0.9702995133110655 1101 100-101 1.175814899958791 1102 1 0.23421263695509964 1102 2 0.10209108513768683 1102 3 0.13926550667849114 1102 4 0.1880136139875006 1102 5 0.23030558588608585 1102 6 0.10506122482389912 1102 7 0.11289408736608664 1102 8 0.18028229985908695 1102 9 0.2637527037218348 1102 10-11 0.24654497201488823 1102 12-13 0.30952483542017006 1102 14-15 0.4641030297087525 1102 16-17 0.3796889912486421 1102 18-19 0.25181313818870876 1102 20-21 0.2892765699001174 1102 22-23 0.22297383003057547 1102 24-25 0.34630317946562883 1102 26-27 0.3307212633681047 1102 28-29 0.23888805479962372 1102 30-31 0.38648690264790275 1102 32-33 0.603665512162884 1102 34-35 0.6520165757011398 1102 36-37 0.6891485975310729 1102 38-39 0.703288390829421 1102 40-41 0.8138889618649117 1102 42-43 0.7779520252998395 1102 44-45 1.0513911146182693 1102 46-47 1.0196402385499042 1102 48-49 0.9434657316270858 1102 50-51 1.0922710094804842 1102 52-53 1.331412956079454 1102 54-55 1.454096652325056 1102 56-57 1.405236125077792 1102 58-59 1.1396827385999657 1102 60-61 1.196079866632651 1102 62-63 1.5678233341743066 1102 64-65 1.2971299135811094 1102 66-67 1.259232126269147 1102 68-69 0.946091543261641 1102 70-71 1.2697655777442733 1102 72-73 1.472849554481364 1102 74-75 1.2764387331939737 1102 76-77 1.0452955565360114 1102 78-79 1.2081443694488812 1102 80-81 1.145960810416753 1102 82-83 1.1960312572817884 1102 84-85 1.1730216290961657 1102 86-87 1.0782397635844099 1102 88-89 1.2751232504202186 1102 90-91 1.4908998987781317 1102 92-93 1.2549766201340127 1102 94-95 1.1965947223064717 1102 96-97 1.2911240821943188 1102 98-99 1.5460037386631775 1102 100-101 1.891190486813251 1103 1 0.004731007114898489 1103 2 -0.16581066788810972 1103 3 -0.05077842664622523 1103 4 0.10629547641589454 1103 5 0.049694395809851244 1103 6 0.13304179940286787 1103 7 0.1053888110599317 1103 8 0.13667739827835135 1103 9 0.23300476040444096 1103 10-11 0.2324416210220761 1103 12-13 0.18352072205495773 1103 14-15 0.30681257443890786 1103 16-17 0.3535847357462103 1103 18-19 0.28153812419034097 1103 20-21 0.29856932951591375 1103 22-23 0.3074889482629004 1103 24-25 0.3935611154608054 1103 26-27 0.46577585698485535 1103 28-29 0.3745214268976511 1103 30-31 0.5111934754178549 1103 32-33 0.631815682296839 1103 34-35 0.4824344806845531 1103 36-37 0.5774281986854035 1103 38-39 0.5900039440878047 1103 40-41 0.616969678581107 1103 42-43 0.759972204478899 1103 44-45 0.9610828275872123 1103 46-47 0.9610786242155811 1103 48-49 1.0345116247226613 1103 50-51 0.872405178408421 1103 52-53 0.8276737778910572 1103 54-55 1.0894231372283656 1103 56-57 1.1504659135948216 1103 58-59 1.0616687833085834 1103 60-61 1.097076442417631 1103 62-63 1.2402993215973126 1103 64-65 0.9054772452777087 1103 66-67 1.178854601179772 1103 68-69 1.3194171236553238 1103 70-71 1.2073813334405301 1103 72-73 1.0038209332124666 1103 74-75 1.0756241361314736 1103 76-77 0.9949552886288764 1103 78-79 1.2145943845240446 1103 80-81 1.3335361862189679 1103 82-83 1.530449463768413 1103 84-85 1.6248968713755296 1103 86-87 1.4588094969694332 1103 88-89 1.5725534739633105 1103 90-91 1.7645275207792501 1103 92-93 1.6951040052397524 1103 94-95 1.615587270870023 1103 96-97 1.589020020515374 1103 98-99 1.5526045053427673 1103 100-101 1.247744951214333 1104 1 0.23937337235989276 1104 2 0.08111663794563384 1104 3 0.16480039540013536 1104 4 0.12357609830818461 1104 5 0.1541309810048901 1104 6 0.24524328410068108 1104 7 0.2600915213337558 1104 8 0.22045719144780662 1104 9 0.2553306702487319 1104 10-11 0.2705701664384321 1104 12-13 0.23890070898215043 1104 14-15 0.22672264050329005 1104 16-17 0.33709052513388826 1104 18-19 0.3473136220076469 1104 20-21 0.35970806472145966 1104 22-23 0.25261669997287584 1104 24-25 0.25821229318100336 1104 26-27 0.03373258831524595 1104 28-29 0.08863539379542829 1104 30-31 0.19869347541785487 1104 32-33 0.40626738292947095 1104 34-35 0.43953494290179407 1104 36-37 0.32392801193095977 1104 38-39 0.25244529152110573 1104 40-41 0.1308799664163942 1104 42-43 0.06775957287537437 1104 44-45 0.18882257360117194 1104 46-47 0.5397635227845754 1104 48-49 0.7056239225026175 1104 50-51 0.36292739051477696 1104 52-53 0.4300896216709731 1104 54-55 0.19720671217525165 1104 56-57 0.4858681359726802 1104 58-59 0.32351601817563846 1104 60-61 0.4410445657689479 1104 62-63 0.5613768947233524 1104 64-65 0.5390125068506535 1104 66-67 0.6894412435683677 1104 68-69 0.4012038967720173 1104 70-71 0.4120262665590957 1104 72-73 0.6673492498546203 1104 74-75 0.6543192478339748 1104 76-77 0.44969651700621327 1104 78-79 0.3833853829599754 1104 80-81 0.540913862059945 1104 82-83 0.542441141523625 1104 84-85 0.5446931339522756 1104 86-87 0.6872378999855151 1104 88-89 0.5766051699270811 1104 90-91 0.6946011137012569 1104 92-93 0.7037545293194043 1104 94-95 0.9052550814076419 1104 96-97 1.0755065858677568 1104 98-99 1.0725376239084916 1104 100-101 0.41533663597283876 1105 1 0.12203391490528759 1105 2 -0.15300161797363643 1105 3 -0.09806241565778606 1105 4 0.07905761812532575 1105 5 0.14209837652824575 1105 6 -0.02329080827867358 1105 7 0.0571329669714018 1105 8 -0.03840553335488295 1105 9 0.07822712084605143 1105 10-11 0.16991570488669794 1105 12-13 0.223655097216934 1105 14-15 0.2116064115819185 1105 16-17 0.21442480727473878 1105 18-19 0.1723987990465119 1105 20-21 0.20390416598509375 1105 22-23 0.245403606429079 1105 24-25 0.29092997981575053 1105 26-27 0.10243487839158405 1105 28-29 0.17142963194611838 1105 30-31 0.19163132852519738 1105 32-33 0.2719733819806578 1105 34-35 0.21374570755440203 1105 36-37 -0.024516159371927415 1105 38-39 0.3676175863414741 1105 40-41 0.32849069656690943 1105 42-43 0.26182844767591007 1105 44-45 0.43075770966879645 1105 46-47 0.9184724976411687 1105 48-49 0.9555807948880073 1105 50-51 0.8897365939477382 1105 52-53 0.9488932616416434 1105 54-55 0.626930264165594 1105 56-57 0.9215486897312459 1105 58-59 0.8943477342234232 1105 60-61 1.067807407016197 1105 62-63 0.9994995496593049 1105 64-65 0.9261583213156541 1105 66-67 0.9873991510165041 1105 68-69 1.0846687693299195 1105 70-71 1.1015074413553236 1105 72-73 1.1614785033156139 1105 74-75 1.130809306918806 1105 76-77 1.055968350361109 1105 78-79 1.1985458176863268 1105 80-81 1.3516483253367824 1105 82-83 1.4739814442794774 1105 84-85 1.481279367617688 1105 86-87 1.3195264668548816 1105 88-89 1.4933634827747966 1105 90-91 1.3857534835636791 1105 92-93 1.3880334356030986 1105 94-95 1.385432551541772 1105 96-97 1.6819293658737955 1105 98-99 1.61444688340735 1105 100-101 1.64671089741244 1106 1 0.21484961195827168 1106 2 0.0035566351947906583 1106 3 0.05515181552254944 1106 4 0.027805518568136733 1106 5 0.1268288697333162 1106 6 0.1506899521427698 1106 7 0.1104388151925022 1106 8 0.1651446269749357 1106 9 0.2569261588671239 1106 10-11 0.19830553539516416 1106 12-13 0.15268242963409762 1106 14-15 0.2521851258894401 1106 16-17 0.2775794873785742 1106 18-19 0.22935405939159637 1106 20-21 0.15660305131110874 1106 22-23 0.1425176696448318 1106 24-25 0.12589333299944627 1106 26-27 0.0659483919050956 1106 28-29 0.17817875394947436 1106 30-31 0.12166644839082608 1106 32-33 0.26907599306632335 1106 34-35 0.15733633207723585 1106 36-37 0.11205812677863491 1106 38-39 0.17692435073298185 1106 40-41 0.11694694942993777 1106 42-43 0.24515796363253628 1106 44-45 0.27498583610031346 1106 46-47 -0.3440065067869895 1106 48-49 -0.33265405012649296 1106 50-51 -0.5778882342703113 1106 52-53 -0.7383719696913857 1106 54-55 -0.5844073446296392 1106 56-57 -0.5295468973812945 1106 58-59 -0.26668066705921234 1106 60-61 -0.51033429400686 1106 62-63 -0.12596235292124192 1106 64-65 -0.22216966769030222 1106 66-67 -0.10175949915909399 1106 68-69 -0.19538162004250736 1106 70-71 -0.4866739603854029 1106 72-73 -0.4020235580678033 1106 74-75 -0.5910146356678467 1106 76-77 -0.5580986827737213 1106 78-79 -0.6083827210218722 1106 80-81 -0.7577347865886992 1106 82-83 -0.2976592642256328 1106 84-85 -0.302019609325356 1106 86-87 -0.459100041008913 1106 88-89 -0.034171255353161456 1106 90-91 -0.07353037656763917 1106 92-93 -0.20209101087727888 1106 94-95 -0.04343826843074439 1106 96-97 0.01519780380325031 1106 98-99 0.11420257129863032 1106 100-101 0.6973254950609338 1107 1 0.17642449742394462 1107 2 0.1202094654994994 1107 3 0.19089267583678549 1107 4 0.18790145067772102 1107 5 0.20821742552460165 1107 6 0.15829072697903968 1107 7 0.26842854553718354 1107 8 0.10999596295255287 1107 9 0.18611165835979193 1107 10-11 0.21559791309709908 1107 12-13 0.22291272819204266 1107 14-15 0.1321777464964029 1107 16-17 0.28949566977257035 1107 18-19 0.2865597292252602 1107 20-21 0.20109617925132994 1107 22-23 0.28240636379973694 1107 24-25 0.30734645847986997 1107 26-27 0.22302645973401525 1107 28-29 0.37571126940007815 1107 30-31 0.13470030135641764 1107 32-33 -0.0014956024199506146 1107 34-35 -0.0992712994450784 1107 36-37 -0.03928400033141344 1107 38-39 0.2288476050453241 1107 40-41 0.30414677724410666 1107 42-43 0.29343456788184596 1107 44-45 -0.22248364703470003 1107 46-47 -0.18091577100000222 1107 48-49 -0.44196745909860624 1107 50-51 -0.5152956075138562 1107 52-53 -0.47205889894144804 1107 54-55 -0.4443034181775971 1107 56-57 -0.5837177918252223 1107 58-59 -0.49807383497115154 1107 60-61 -0.45946260320343413 1107 62-63 -0.3077014298821581 1107 64-65 -0.18524041762111665 1107 66-67 -0.3004327457845619 1107 68-69 -0.2828858478197738 1107 70-71 -0.27322501656103526 1107 72-73 -0.3252624966651041 1107 74-75 -0.08598896152954438 1107 76-77 -0.05397698428341968 1107 78-79 -0.13913326678948224 1107 80-81 -0.09759580801969037 1107 82-83 -0.12879569075455422 1107 84-85 -0.3188184901174118 1107 86-87 -0.3934249802826564 1107 88-89 -0.27884733074596113 1107 90-91 -0.009823153986694422 1107 92-93 0.12925757320168074 1107 94-95 0.30647871339749955 1107 96-97 0.28834603736104114 1107 98-99 0.2052135481457853 1107 100-101 0.3047125749736104 1108 1 0.10769504503742411 1108 2 -0.028174929424920947 1108 3 0.12413465478919505 1108 4 0.1020644185365569 1108 5 0.04747739806130369 1108 6 0.157151171291801 1108 7 0.22280928248784448 1108 8 0.2646678563813083 1108 9 0.15461149027532173 1108 10-11 0.18055372682533033 1108 12-13 0.19120459119465494 1108 14-15 0.23278342034416966 1108 16-17 0.3106586433291838 1108 18-19 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pass #Quality Count 2 2.0 3 2.0 4 4.0 5 4.0 6 43.0 7 83.0 8 181.0 9 379.0 10 757.0 11 1337.0 12 2449.0 13 3297.0 14 4196.0 15 5964.0 16 8518.0 17 12540.0 18 10595.0 19 5419.0 20 4163.0 21 4091.0 22 3969.0 23 4125.0 24 4692.0 25 5362.0 26 6462.0 27 7765.0 28 9398.0 29 11857.0 30 14916.0 31 18528.0 32 23823.0 33 31431.0 34 44214.0 35 64424.0 36 96419.0 37 138008.0 38 101951.0 39 8508.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.936854677846696 20.043107368267883 15.495613476660786 19.524424477224635 2 15.30762865724842 22.82216975270287 39.337641357027465 22.532560233021247 3 19.82490278270305 27.618350503590143 31.293967526543433 21.26277918716338 4 12.15304087434609 17.80880044129503 38.833053482775554 31.20510520158333 5 12.071560091836966 39.49368431421579 33.58596076470187 14.848794829245376 6 28.733385161173974 38.67857191567519 17.25154839462506 15.33649452852577 7 25.924719189665936 35.881438330837916 21.20853008747098 16.98531239202517 8 26.45432406342394 32.67305876701264 20.0857129434728 20.78690422609062 9 27.296753788963702 16.891632834604025 21.3971694724926 34.41444390393967 10-11 24.468802519257377 27.325016631887454 26.988362982798307 21.21781786605686 12-13 27.154054119586828 25.516084937684884 27.73128727995732 19.598573662770963 14-15 23.585986479298416 25.76540779569526 28.72148900476907 21.927116720237255 16-17 20.17860904380665 29.232720643392636 28.961836827166277 21.626833485634446 18-19 19.748074262324643 27.06201307213314 33.51238185298169 19.677530812560523 20-21 21.603630472570657 25.346551308688674 33.265212719687085 19.784605499053587 22-23 20.71757312347604 25.601362072022514 33.285672936016866 20.39539186848458 24-25 21.260097885363948 25.747760677180615 33.533428253392614 19.458713184062827 26-27 20.972279789016333 25.300612325465266 34.32417257257773 19.40293531294067 28-29 20.078396189457205 26.192177506067633 34.15523294185755 19.574193362617613 30-31 20.399619871562663 25.851310366987633 33.56050604977136 20.188563711678338 32-33 20.472548339140044 26.1279890402489 33.431105264832865 19.968357355778192 34-35 21.180011457010448 25.706894513668093 33.21441885981529 19.898675169506166 36-37 21.45195315341529 25.243083339395262 33.48518464634708 19.819778860842366 38-39 20.804318472167708 26.034646585730677 33.31721887725179 19.843816064849825 40-41 20.623354737220183 26.315338812364647 33.260033734990884 19.801272715424286 42-43 20.442244319451884 26.888142435950574 31.983378330149588 20.686234914447958 44-45 21.048535891403265 26.153234147505767 31.855244763192893 20.94298519789808 46-47 21.768365220685737 27.095586285281303 30.75340026520174 20.38264822883122 48-49 21.87935451460162 27.05911948541808 30.292987086200352 20.76853891377995 50-51 21.23318810380754 27.67508997916272 30.528130327713583 20.563591589316157 52-53 21.64192449444741 28.371562969788076 29.424494447407984 20.562018088356528 54-55 20.88702649864741 27.615652160734715 29.77115837810395 21.72616296251392 56-57 21.6119125682232 27.89962333746796 29.199794809467555 21.288669284841284 58-59 22.295199725399833 27.26543243085554 29.299095198513037 21.140272645231587 60-61 21.51270590214292 27.555558249686868 30.569558724022368 20.362177124147845 62-63 21.528178736340255 27.3503243786342 30.499320327879285 20.622176557146258 64-65 21.245127872509382 28.19014481508647 29.893495141511433 20.671232170892715 66-67 21.319898496157993 27.681467668571315 29.45422363124009 21.544410204030605 68-69 21.133862825950896 27.82132124623225 29.82283061843344 21.221985309383413 70-71 21.828722366020283 28.029357030714863 29.1961671586783 20.94575344458656 72-73 21.785591697840427 27.425889541452076 29.46460436157372 21.323914399133777 74-75 21.21603610388961 29.0686017850391 28.73975087649375 20.97561123457754 76-77 21.543084805712613 27.976747040718443 28.8700249896571 21.610143163911843 78-79 21.38695649867816 28.132309606418776 28.900710966370223 21.580022928532838 80-81 21.688967290902287 28.530477519788676 28.501304090190605 21.279251099118433 82-83 21.486185547012635 28.347531826320466 28.801332988821294 21.364949637845605 84-85 21.125952665645237 28.387503997066037 28.55541865762231 21.931124679666414 86-87 21.152040735142016 28.46815104433053 28.45489090695551 21.92491731357194 88-89 21.336031836477378 28.322992944086128 28.05347097618932 22.287504243247174 90-91 21.153276408540084 27.715792273251132 28.564435920963998 22.566495397244786 92-93 21.30033324392273 28.137743852263377 28.664508688740476 21.897414215073415 94-95 21.135166959271924 27.935606132359613 28.63285781506085 22.29636909330761 96-97 20.725417261166427 27.758560986014636 29.09011416668624 22.42590758613269 98-99 20.960180521636605 28.37025121028452 27.903792515292892 22.765775752785984 100-101 20.364070055904694 28.51904836233639 28.07638742606532 23.040494155693594 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1223.0 1 863.5 2 561.0 3 625.5 4 535.5 5 516.0 6 839.0 7 1688.5 8 2639.5 9 2908.0 10 2469.5 11 2019.5 12 1948.5 13 2027.5 14 2079.0 15 2112.0 16 2221.5 17 2345.0 18 2430.5 19 2551.5 20 2672.0 21 2830.0 22 3125.0 23 3502.0 24 3900.0 25 4502.0 26 5370.0 27 6158.0 28 6750.5 29 7752.0 30 9153.0 31 10344.0 32 11932.5 33 13506.0 34 14889.5 35 16045.0 36 17508.5 37 18953.5 38 19419.5 39 19068.5 40 18239.5 41 18149.5 42 18299.5 43 18978.0 44 19750.5 45 19995.5 46 19937.0 47 23125.0 48 33994.5 49 32908.5 50 21909.5 51 18087.5 52 16719.5 53 16659.5 54 16462.0 55 15769.5 56 14667.0 57 13230.5 58 12481.0 59 11624.5 60 9888.0 61 8373.0 62 7048.0 63 5702.0 64 4344.0 65 3303.0 66 2456.0 67 1931.0 68 1384.0 69 811.0 70 570.0 71 439.0 72 322.0 73 241.0 74 213.5 75 170.5 76 122.0 77 66.0 78 40.0 79 33.0 80 20.0 81 14.5 82 9.0 83 5.0 84 4.0 85 2.0 86 0.5 87 0.5 88 1.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019549097786405647 2 0.003485498054940542 3 0.002273150905396006 4 1.5154339369306705E-4 5 0.0018185207243168044 6 0.0 7 1.5154339369306705E-4 8 0.0 9 0.0 10-11 0.0 12-13 7.577169684653352E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.002727781086475207 22-23 0.0 24-25 0.003561269751787076 26-27 0.002348922602242539 28-29 0.003561269751787076 30-31 0.015154339369306704 32-33 0.0012123471495445364 34-35 0.0028793244801682736 36-37 0.003182411267554408 38-39 0.0021216075117029384 40-41 0.004243215023405877 42-43 0.002348922602242539 44-45 6.819452716188018E-4 46-47 3.030867873861341E-4 48-49 2.2731509053960054E-4 50-51 3.030867873861341E-4 52-53 7.577169684653352E-4 54-55 0.0048493885981781455 56-57 3.788584842326676E-4 58-59 0.0012881188463910698 60-61 9.850320590049358E-4 62-63 0.0 64-65 1.5154339369306705E-4 66-67 7.577169684653352E-5 68-69 0.0 70-71 1.5154339369306705E-4 72-73 7.577169684653352E-5 74-75 5.304018779257346E-4 76-77 6.061735747722682E-4 78-79 7.577169684653352E-5 80-81 0.004546301810792012 82-83 9.850320590049358E-4 84-85 0.002727781086475207 86-87 6.819452716188018E-4 88-89 7.577169684653352E-4 90-91 7.577169684653352E-5 92-93 0.0 94-95 7.577169684653352E-5 96-97 0.0012881188463910698 98-99 0.001742749027470271 100-101 6.061735747722682E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 659877.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.140151339760834 #Duplication Level Percentage of deduplicated Percentage of total 1 74.23670394925078 34.995294591327024 2 13.422139916218029 12.654434139079255 3 4.9443908786508475 6.9923800290760205 4 2.2893122719716574 4.316741078588626 5 1.2949151688926916 3.052124851687672 6 0.7930402880553772 2.2430423518474805 7 0.5221566268969023 1.7230179690485306 8 0.3940469019110506 1.4860344472840654 9 0.2929810463278835 1.2430053777220118 >10 1.6129722881248216 14.177015753695448 >50 0.12358055341263961 3.912763572765681 >100 0.06340781410670408 5.353151625621763 >500 0.004529129579050101 1.3586470238921748 >1k 0.004852638834696537 3.6527459329066527 >5k 9.705277669393074E-4 2.83960125545766 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7848 1.1893125537031901 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5681 0.8609180195703139 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5091 0.7715074172914043 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4420 0.6698218001233563 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2069 0.31354328155095573 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1818 0.27550588973399587 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1684 0.2551990749791249 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1624 0.24610647135754085 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1421 0.2153431624378483 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1409 0.21352464171353144 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1385 0.20988760026489786 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1335 0.2023104305802445 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1210 0.18336750636861113 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1203 0.18230670261275964 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1143 0.17321409899117562 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1143 0.17321409899117562 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1047 0.15866593319664118 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1041 0.1577566728344828 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 956 0.1448754843705721 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 956 0.1448754843705721 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 717 0.10865661327792908 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 680 0.10304950771128558 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 7.577169684653352E-5 0.0 20-21 0.0 0.0 0.0 1.5154339369306705E-4 0.0 22-23 0.0 0.0 0.0 5.304018779257347E-4 0.0 24-25 0.0 0.0 0.0 0.0015154339369306703 0.0 26-27 0.0 0.0 0.0 0.0027277810864752066 0.0 28-29 0.0 0.0 0.0 0.007198311200420684 0.0 30-31 0.0 0.0 0.0 0.014775480885074037 0.0 32-33 0.0 0.0 0.0 0.02629277880574713 0.0 34-35 0.0 0.0 0.0 0.036900816364261825 0.0 36-37 0.0 0.0 0.0 0.045917648288999316 0.0 38-39 0.0 0.0 0.0 0.05508602360742987 0.0 40-41 0.0 0.0 0.0 0.07114962333889498 0.0 42-43 0.0 0.0 0.0 0.08994100415683529 0.0 44-45 0.0 0.0 0.0 0.10683809255361226 0.0 46-47 0.0 0.0 0.0 0.12828148276118123 0.0 48-49 0.0 0.0 0.0 0.14336005043364142 0.0 50-51 0.0 0.0 0.0 0.16510652742859655 0.0 52-53 0.0 0.0 0.0 0.22731509053960056 0.0 54-55 0.0 0.0 0.0 0.31430099851942106 0.0 56-57 0.0 0.0 0.0 0.38779954446055853 0.0 58-59 0.0 0.0 0.0 0.49766850488803216 0.0 60-61 0.0 0.0 0.0 0.6383007742351984 0.0 62-63 0.0 0.0 0.0 0.7239227916717812 0.0 64-65 0.0 0.0 0.0 0.7804484775192952 0.0 66-67 0.0 0.0 0.0 0.9523744576640798 0.0 68-69 0.0 0.0 0.0 1.1436222205047306 0.0 70-71 0.0 0.0 0.0 1.2231825021935907 0.0 72-73 0.0 0.0 0.0 1.3898802352559643 0.0 74-75 0.0 0.0 0.0 1.6251513539644509 0.0 76-77 0.0 0.0 0.0 1.8477685992995665 0.0 78-79 0.0 0.0 0.0 2.0410621979550734 0.0 80-81 0.0 0.0 0.0 2.2607243471131744 0.0 82-83 0.0 0.0 0.0 2.4746278473109387 0.0 84-85 0.0 0.0 0.0 2.7700616933155726 0.0 86-87 0.0 0.0 0.0 3.299326995788609 0.0 88-89 0.0 0.0 0.0 3.9005754102658527 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGG 175 3.6379788E-12 32.577404 1 TCGACGT 35 0.008393811 27.141665 56-57 TAAGGTG 230 1.3460522E-10 24.783401 5 AGTACTT 6050 0.0 24.180758 12-13 AGGTAAG 140 1.7765102E-5 23.752556 2 GTAAGGT 200 2.050001E-8 23.750757 4 GAGTACT 5955 0.0 23.569494 12-13 GTACATG 4680 0.0 23.449816 1 ATGGGAG 850 0.0 22.912497 5 TACATGG 4850 0.0 22.332302 2 ACATGGG 4630 0.0 22.264816 3 CCTTGCG 75 3.0337931E-5 22.166534 54-55 GTACTTT 6325 0.0 21.966272 14-15 GTACTAA 110 0.0028730212 21.59487 1 TACTTTT 6750 0.0 21.462835 14-15 TTAGACT 155 3.902218E-5 21.452297 4 CATGGGG 1430 0.0 20.92724 4 TTTAGGC 230 8.99563E-8 20.654398 3 TAGGCAT 230 9.002906E-8 20.652834 5 GGTGTGC 465 0.0 20.429213 8 >>END_MODULE