FastQCFastQC Report
Thu 26 May 2016
SRR936906_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936906_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568952
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70561.2401749180950237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51580.9065791138795539No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49040.861935629016156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39260.6900406361169308No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT19300.3392201802612522No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15220.2675093856775264No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14960.2629395801403282No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14200.24958168703159492No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14100.24782406951728794No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12790.22479928007986613No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT12180.21407781324259337No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC11680.20528972567105835No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC11340.19931382612241455No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT11050.1942167353309242No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC10900.19158030905946372No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10300.18103460397362167No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10010.17593751318213136No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA9250.1625796200733981No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG9140.1606462408076604No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT8430.14816715645608064No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT6060.1065116213670046No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC5750.10106300707265288No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG500.001616709237.992444
TGAACCG804.496055E-429.6815955
GTCCTAT1004.9406583E-528.5068591
GATATAG1554.56821E-827.5872821
GTAATAG1151.2816067E-424.7798583
GAGTACT53050.023.9477512-13
GGGTAGG1801.942899E-723.7557161
GTACATG39450.023.0031
CATGGGG10750.022.5304034
AGTACTT53750.022.31049312-13
GTACTTT56550.022.25854914-15
TACATGG40850.021.2785782
GTGTAGC1805.1504303E-621.1161921
ACATGGG39350.021.001483
TTAGGCA1856.553797E-620.5364554
ACTTTTT62300.020.39479316-17
TACTTTT59300.020.06488214-15
GTAATAC1200.00476365419.789473
ATGGGAT9850.019.7676415
AGGCTTA2201.346003E-619.4262456
Produced by FastQC (version 0.11.5)