FastQCFastQC Report
Thu 26 May 2016
SRR936905_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936905_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580737
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53060.9136665995106218No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35560.6123253727590975No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30970.5332878738568405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26610.4582108596490322No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24040.4139567480632369No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18490.31838853043632487No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14730.25364321543142593No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14290.24606663601595904No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA12380.2131773935533641No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT11370.19578569989513328No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11010.1895866803733876No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT10770.18545400069222384No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT10640.18321546586492682No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC8990.15480329305692594No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC8790.1513593933226228No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8730.15032622340233187No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7270.12518575534191898No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7220.12432478040834319No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA6880.11847015086002786No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC6670.11485405613900956No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6450.11106576643127612No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG6310.10865503661726392No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA5950.10245601709551828No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT5940.10228382210880312No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTG550.002570538334.567461
GTCCTAT953.4727684E-530.019111
GTACATG41650.027.8448521
TACATGG42200.026.4659882
CATATAG1457.8385165E-726.2213293
ACATGGG40850.025.9445133
CATGGGG13250.025.8255354
GAGTACT35250.024.1328112-13
ATGGGAT10300.023.532375
TAAGGTG2252.7885108E-923.235015
AGTACTT36000.023.10199712-13
GTATAGA1652.4130477E-623.0449711
GTCTAGG1252.2443362E-422.8145221
GTACTTT38150.022.29353314-15
CATGGGA27050.021.962184
ATGGGGA6750.020.4186445
GTGCATA2801.5588739E-920.3701081
GTATAGG1404.8269847E-420.3701081
AGGTGTG2351.124954E-720.2204117
GTAAGGT2151.0712356E-619.894674
Produced by FastQC (version 0.11.5)