##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936905_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 580737 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.94953998109299 33.0 31.0 34.0 30.0 34.0 2 32.06283222870249 33.0 31.0 34.0 30.0 34.0 3 32.0582449542564 34.0 31.0 34.0 30.0 34.0 4 35.6425094319804 37.0 35.0 37.0 33.0 37.0 5 35.63772241134971 37.0 35.0 37.0 33.0 37.0 6 35.76310274702662 37.0 35.0 37.0 35.0 37.0 7 35.717503792594584 37.0 35.0 37.0 33.0 37.0 8 35.688005069420406 37.0 35.0 37.0 33.0 37.0 9 37.300211627638674 39.0 37.0 39.0 34.0 39.0 10-11 37.18254562736661 39.0 37.0 39.0 34.0 39.0 12-13 37.09997727026175 39.0 37.0 39.0 33.0 39.0 14-15 38.22992593893621 40.0 38.0 41.0 33.5 41.0 16-17 38.193913940389535 40.0 38.0 41.0 33.0 41.0 18-19 38.21574309885542 40.0 38.0 41.0 33.5 41.0 20-21 38.127349385349994 40.0 38.0 41.0 33.5 41.0 22-23 37.99492713569137 40.0 38.0 41.0 33.0 41.0 24-25 38.082183501309544 40.0 38.0 41.0 33.0 41.0 26-27 37.99641662232646 40.0 38.0 41.0 33.0 41.0 28-29 37.90135293601062 40.0 38.0 41.0 33.0 41.0 30-31 37.671067970527105 40.0 37.0 41.0 33.0 41.0 32-33 37.39190804098929 40.0 36.5 41.0 32.5 41.0 34-35 37.15528629999466 39.0 36.0 41.0 32.0 41.0 36-37 36.916267604784956 39.0 35.5 41.0 31.0 41.0 38-39 36.701639468468514 39.0 35.0 41.0 31.0 41.0 40-41 36.48676853722081 39.0 35.0 41.0 30.5 41.0 42-43 36.1990875387654 39.0 35.0 40.5 30.0 41.0 44-45 35.8108283095446 39.0 35.0 40.0 29.5 41.0 46-47 35.29006159414675 38.5 35.0 40.0 26.5 41.0 48-49 34.871837854312716 38.5 35.0 40.0 24.0 41.0 50-51 34.11786832938146 38.0 34.0 39.5 23.5 40.5 52-53 34.294908883022785 38.0 34.0 39.5 24.0 40.5 54-55 34.66384266888454 39.0 35.0 40.0 23.5 41.0 56-57 34.515827302203924 38.0 34.5 40.0 23.0 41.0 58-59 34.26944899326201 38.0 34.0 40.0 22.5 41.0 60-61 33.94628291291927 38.0 34.0 40.0 22.0 41.0 62-63 33.614706829425366 37.0 34.0 40.0 20.0 41.0 64-65 33.26423234613948 37.0 33.0 40.0 18.5 41.0 66-67 32.863261682999365 36.0 33.0 39.0 16.5 41.0 68-69 32.44312916173759 36.0 32.5 39.0 12.0 41.0 70-71 31.98913794023801 35.0 32.0 38.5 8.0 40.0 72-73 31.58469927006545 35.0 31.5 38.0 6.5 40.0 74-75 31.14505791778378 35.0 31.0 37.0 2.0 39.5 76-77 30.701237393174537 35.0 31.0 36.5 2.0 39.0 78-79 30.257342824721 35.0 30.5 36.0 2.0 39.0 80-81 29.794786624582212 34.5 30.0 36.0 2.0 37.5 82-83 29.358212065013937 34.0 29.5 35.0 2.0 37.0 84-85 28.947279921892353 34.0 29.0 35.0 2.0 37.0 86-87 28.613652135131737 34.0 29.0 35.0 2.0 36.0 88-89 28.222060932917998 34.0 28.5 35.0 2.0 36.0 90-91 27.7881089029974 34.0 27.0 35.0 2.0 35.5 92-93 27.40868672049482 34.0 27.0 35.0 2.0 35.0 94-95 27.000157558412845 34.0 25.5 35.0 2.0 35.0 96-97 26.4691004706089 33.5 25.0 35.0 2.0 35.0 98-99 25.79813323414902 33.0 23.0 35.0 2.0 35.0 100-101 23.35730804133368 30.5 8.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13525413781870554 1101 2 0.19043147716402586 1101 3 0.17182127631705413 1101 4 0.19323458479436795 1101 5 0.008200790558937854 1101 6 0.06901215706309927 1101 7 -0.01495367025168548 1101 8 -0.17868602125523125 1101 9 -0.053205275892452164 1101 10-11 0.23900890773695238 1101 12-13 0.20378949028489046 1101 14-15 0.29375433651138394 1101 16-17 0.22882142572431974 1101 18-19 0.20436801353607592 1101 20-21 0.16289019045198216 1101 22-23 0.19139346901338428 1101 24-25 0.25306625929767534 1101 26-27 0.30289898281139216 1101 28-29 0.1386484641851169 1101 30-31 0.20688869890509665 1101 32-33 0.23635742028147178 1101 34-35 0.3333333681349373 1101 36-37 0.24885940855130428 1101 38-39 0.2867332267184466 1101 40-41 0.5700808458885618 1101 42-43 0.5624468445331772 1101 44-45 0.5067849572868752 1101 46-47 0.5656192481851079 1101 48-49 0.8209195949438595 1101 50-51 0.7188159249088102 1101 52-53 0.9393182500720769 1101 54-55 0.8963427327804752 1101 56-57 0.6300210383604394 1101 58-59 0.7744801724713994 1101 60-61 0.9992909227815119 1101 62-63 0.9994947471162448 1101 64-65 0.8569588889567044 1101 66-67 0.7624381299522014 1101 68-69 0.717965943649503 1101 70-71 0.8474008701859468 1101 72-73 0.8045485946459117 1101 74-75 0.8499925958109067 1101 76-77 0.8237745048669609 1101 78-79 0.9717894624799506 1101 80-81 1.1053713157193563 1101 82-83 1.0653598875923862 1101 84-85 1.237009299596373 1101 86-87 1.0501238899216716 1101 88-89 1.193938485568438 1101 90-91 0.9656352937916743 1101 92-93 0.928920178829312 1101 94-95 0.7999961547240382 1101 96-97 0.85374717974085 1101 98-99 0.3856477252395756 1101 100-101 0.29870409740658843 1102 1 0.23651986650936152 1102 2 0.15802540330166437 1102 3 -7.757254056457441E-4 1102 4 0.0458443720307784 1102 5 -0.05546846604148925 1102 6 0.1164486429795204 1102 7 0.15565674511025662 1102 8 0.05353272996431713 1102 9 0.12825424253225037 1102 10-11 0.09389611460396452 1102 12-13 -0.12737854444413443 1102 14-15 0.07122228445501833 1102 16-17 0.2055389680809725 1102 18-19 0.21015494518003663 1102 20-21 0.27609495008247364 1102 22-23 0.12119672448765328 1102 24-25 -0.054788654855407515 1102 26-27 0.06453184901507569 1102 28-29 0.04072220760885159 1102 30-31 -0.06412144959642063 1102 32-33 0.06233412760285262 1102 34-35 -0.17137199824993843 1102 36-37 -0.09915465551377878 1102 38-39 0.04835773030414714 1102 40-41 -0.14130383639700028 1102 42-43 -0.05234588333422607 1102 44-45 -0.29462981183982606 1102 46-47 -0.09244166512428365 1102 48-49 -0.4647632313980168 1102 50-51 -0.33266342221231326 1102 52-53 -0.17689519367259265 1102 54-55 -0.1047704245909884 1102 56-57 -0.20792257529404878 1102 58-59 -0.1798506415458334 1102 60-61 -0.2969891989038729 1102 62-63 -0.4414224276568959 1102 64-65 -0.3065321509627239 1102 66-67 -0.5533241040933206 1102 68-69 -0.30678926393854766 1102 70-71 -0.37573236118538844 1102 72-73 -0.39949092151158894 1102 74-75 -0.15386721697795025 1102 76-77 0.04253408703339545 1102 78-79 -0.05820167314498548 1102 80-81 -0.043566910550786986 1102 82-83 0.2064502614757444 1102 84-85 0.10741659625931987 1102 86-87 -0.07639322835733964 1102 88-89 -0.1069977559737012 1102 90-91 -0.22720481852757857 1102 92-93 -0.059729890208743086 1102 94-95 0.21491487936904363 1102 96-97 -0.003891328442435338 1102 98-99 -0.1748641784825331 1102 100-101 -0.13776416459980467 1103 1 0.23070615138960449 1103 2 0.1701058769267476 1103 3 0.11186807999479953 1103 4 0.12412862908063005 1103 5 0.018558003172060467 1103 6 0.05384255702837493 1103 7 0.10175893201832764 1103 8 0.05677373264568075 1103 9 0.22912506981817415 1103 10-11 0.10408754080913951 1103 12-13 0.12088205595110679 1103 14-15 0.045655057736532 1103 16-17 0.1510374388033 1103 18-19 0.1867904113022263 1103 20-21 0.2936221941847066 1103 22-23 0.25554868794898766 1103 24-25 0.041529188904071646 1103 26-27 0.2753005467920886 1103 28-29 0.43896784322257076 1103 30-31 0.3552245279523092 1103 32-33 0.5654941564664355 1103 34-35 0.3691251604915422 1103 36-37 0.40946854358751494 1103 38-39 0.3384776945526653 1103 40-41 0.6186251936391685 1103 42-43 0.5755773739862207 1103 44-45 0.30240859639941675 1103 46-47 0.4167309259558891 1103 48-49 0.7331265294114786 1103 50-51 0.8078948256372698 1103 52-53 0.942362840160115 1103 54-55 0.9204923815720392 1103 56-57 1.0538565527306858 1103 58-59 0.9689659107324999 1103 60-61 1.0077501269019749 1103 62-63 1.1200530998004083 1103 64-65 1.2052285273313714 1103 66-67 0.9822951508655606 1103 68-69 1.085257262182168 1103 70-71 1.0989218088637251 1103 72-73 0.89968683549316 1103 74-75 0.892839301106882 1103 76-77 0.8418973663295191 1103 78-79 0.8973611256381524 1103 80-81 1.1149183255647657 1103 82-83 0.9941615108915052 1103 84-85 0.9509136812018752 1103 86-87 0.9185310457482565 1103 88-89 1.1489297324849659 1103 90-91 0.963696594311795 1103 92-93 1.0413828335454909 1103 94-95 1.2818751017497974 1103 96-97 0.9978511461174335 1103 98-99 1.0031316477784067 1103 100-101 1.4581313853251636 1104 1 0.27726183547483885 1104 2 0.15761195414514617 1104 3 0.04699272583965808 1104 4 0.274223349863874 1104 5 0.2447688365473013 1104 6 0.30174277329352606 1104 7 0.28586092869890933 1104 8 0.2238888561179948 1104 9 0.25879866032262555 1104 10-11 0.2419335231391031 1104 12-13 0.1545184119671319 1104 14-15 0.2715599345995088 1104 16-17 0.1767482637961848 1104 18-19 0.15667465076978004 1104 20-21 0.11912639375217537 1104 22-23 0.24224796203572652 1104 24-25 0.2936499285644203 1104 26-27 0.14868274934367776 1104 28-29 0.2988146134953311 1104 30-31 0.06625069771207137 1104 32-33 0.22350393031008053 1104 34-35 0.16701783262351455 1104 36-37 0.25821076893915773 1104 38-39 0.19187842214240902 1104 40-41 0.19580796283898394 1104 42-43 0.5025857898858987 1104 44-45 0.3811043834201513 1104 46-47 0.402177035697882 1104 48-49 0.6486531937310858 1104 50-51 0.5763067017793659 1104 52-53 0.5092269116449657 1104 54-55 0.39551920515667405 1104 56-57 0.6617795690427783 1104 58-59 0.7572544822089711 1104 60-61 0.6831114070575453 1104 62-63 0.8065964618014405 1104 64-65 0.523984918888651 1104 66-67 0.7049707196093777 1104 68-69 0.5432783522255917 1104 70-71 0.4439359750693619 1104 72-73 0.4607880274661973 1104 74-75 0.6901493985100267 1104 76-77 0.7888453453400643 1104 78-79 0.8255577425135421 1104 80-81 0.8279688377275853 1104 82-83 1.130119364334142 1104 84-85 0.9043915265974398 1104 86-87 0.6786359930231498 1104 88-89 0.7746631898773799 1104 90-91 0.6928035365907945 1104 92-93 0.4758536545148253 1104 94-95 0.7572686540306428 1104 96-97 0.5854804441777546 1104 98-99 0.8982409538858 1104 100-101 1.0351695586390512 1105 1 0.16435079568800148 1105 2 0.22660305206799336 1105 3 0.24126390612185844 1105 4 0.3125870633017769 1105 5 0.41101159477819493 1105 6 0.3099534759722289 1105 7 0.34426440878530684 1105 8 0.31008861792182074 1105 9 0.12878493376375388 1105 10-11 0.2791567210235968 1105 12-13 0.27611957468740655 1105 14-15 0.06972400735026696 1105 16-17 0.0927386955771965 1105 18-19 0.3157538610889219 1105 20-21 -0.1328594154082836 1105 22-23 0.001859076511188107 1105 24-25 -0.27252015964890575 1105 26-27 -0.14385949010402754 1105 28-29 -0.06456323022375443 1105 30-31 -0.2471766019911925 1105 32-33 0.07724038037972747 1105 34-35 -0.24829384100736007 1105 36-37 -0.2972505847417821 1105 38-39 -0.009008757655323052 1105 40-41 -0.037160001794937614 1105 42-43 0.2754793686824044 1105 44-45 0.059557218560144065 1105 46-47 -0.18261474451568915 1105 48-49 -0.8403630359061722 1105 50-51 -0.6342243949323105 1105 52-53 -0.4013763504313772 1105 54-55 -0.1125123457426156 1105 56-57 -0.23977022001231063 1105 58-59 -0.10037284171762906 1105 60-61 -0.3327750662261266 1105 62-63 -0.21379908514136758 1105 64-65 -0.04211956444770948 1105 66-67 -0.10869124369226313 1105 68-69 0.0063629926099366685 1105 70-71 -0.11687722261327238 1105 72-73 -0.12833115136260176 1105 74-75 -0.2933131259656392 1105 76-77 0.051989030113677615 1105 78-79 0.05280041999996499 1105 80-81 -0.2161378257093034 1105 82-83 -0.07820387594119538 1105 84-85 0.02150632503907346 1105 86-87 -0.14693073218325736 1105 88-89 -0.14592210655573723 1105 90-91 -0.13733534388721225 1105 92-93 0.04903589476997894 1105 94-95 0.2596077202209166 1105 96-97 0.13918055923861772 1105 98-99 -0.16006159562065037 1105 100-101 0.23018257868386272 1106 1 -0.04301624203618104 1106 2 0.10917880964375115 1106 3 0.31381338045704865 1106 4 0.35181866503337744 1106 5 0.3739444952110915 1106 6 0.3140505693550324 1106 7 0.11575482313286756 1106 8 0.3037883705501443 1106 9 0.36927658496968974 1106 10-11 0.3108318910916239 1106 12-13 0.35889245656743896 1106 14-15 0.31976265917463564 1106 16-17 0.3618906745506081 1106 18-19 0.12096799219591503 1106 20-21 0.38944268725096265 1106 22-23 0.4605596021759979 1106 24-25 0.5405982695409506 1106 26-27 0.4723665457648636 1106 28-29 0.6343004061398716 1106 30-31 0.3134294586148698 1106 32-33 0.23805129565491967 1106 34-35 0.5787585708664764 1106 36-37 0.5267483330071343 1106 38-39 0.5373734315840011 1106 40-41 0.5842453785390092 1106 42-43 0.4899969939143105 1106 44-45 0.3001454993269945 1106 46-47 0.24935804459995836 1106 48-49 0.15250570249827433 1106 50-51 0.14984718824640453 1106 52-53 -0.059868156244611725 1106 54-55 0.03013221208051675 1106 56-57 -0.1956298365862139 1106 58-59 -0.12650147498911934 1106 60-61 0.2736720580781409 1106 62-63 -0.05600301216672676 1106 64-65 -0.0015158229511129662 1106 66-67 -0.458045758225353 1106 68-69 -0.29274415024720923 1106 70-71 -0.4959356641717143 1106 72-73 -0.45866896830755977 1106 74-75 -0.30258956381350544 1106 76-77 -0.43925633166739786 1106 78-79 -0.20141262885594102 1106 80-81 -0.42483835137411674 1106 82-83 -0.25692991181009006 1106 84-85 -0.15275542511553653 1106 86-87 0.1425134545823603 1106 88-89 -0.008379589548930255 1106 90-91 -0.0563984236645787 1106 92-93 0.18058737900003408 1106 94-95 0.08132618033222982 1106 96-97 0.22067793091456167 1106 98-99 -0.13926691411168335 1106 100-101 -0.23135885607185713 1107 1 0.21379846466384578 1107 2 0.25366727225147656 1107 3 0.2852444341128759 1107 4 0.3124560017028202 1107 5 0.2789451902347224 1107 6 0.2572323875368525 1107 7 0.3381762854625876 1107 8 0.4496286541197847 1107 9 0.4850883725704662 1107 10-11 0.51272409292649 1107 12-13 0.5026876331034416 1107 14-15 0.36143280095945585 1107 16-17 0.5251951644032644 1107 18-19 0.5361386204972689 1107 20-21 0.45490348791818036 1107 22-23 0.6229397105996881 1107 24-25 0.5094994371806507 1107 26-27 0.321577693943901 1107 28-29 0.6612012957701268 1107 30-31 0.41085092094458986 1107 32-33 0.3978076898723302 1107 34-35 0.3215042617199657 1107 36-37 0.45331734885367325 1107 38-39 0.5236536651119366 1107 40-41 0.490344210899309 1107 42-43 0.5607364990574908 1107 44-45 0.579077595518271 1107 46-47 0.5805507051504151 1107 48-49 0.25771316705207425 1107 50-51 0.09706188104596691 1107 52-53 0.49885612788104794 1107 54-55 0.5715121814994788 1107 56-57 0.36422559849857805 1107 58-59 0.08536952862500158 1107 60-61 0.4271263255232327 1107 62-63 0.6038645858316087 1107 64-65 0.611958947691086 1107 66-67 0.6482737441377253 1107 68-69 0.6766556968475967 1107 70-71 0.546436451480222 1107 72-73 0.5624336866827129 1107 74-75 0.4210412396338086 1107 76-77 0.34681365721222335 1107 78-79 0.45111378204203234 1107 80-81 0.6685154851563269 1107 82-83 0.44206230392524404 1107 84-85 0.5443828150846315 1107 86-87 0.6783610359001244 1107 88-89 0.3752022147324112 1107 90-91 0.4970558437805117 1107 92-93 0.04566417911123821 1107 94-95 -0.04995509573115342 1107 96-97 0.21275292396432377 1107 98-99 0.07728159853774841 1107 100-101 0.06819980392258529 1108 1 0.17723685360192576 1108 2 0.12650038266347252 1108 3 0.18123373388869624 1108 4 0.11707518298496922 1108 5 0.3728283543362281 1108 6 5.588762432182648E-4 1108 7 0.04026169887177389 1108 8 0.2972898639233037 1108 9 0.1110552546231034 1108 10-11 0.20039811285753473 1108 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2316 80-81 -2.606533984172689 2316 82-83 -2.294140250491015 2316 84-85 -2.491558901051363 2316 86-87 -2.41511800709322 2316 88-89 -2.3984531189606955 2316 90-91 -2.093697888905222 2316 92-93 -2.8223426566505907 2316 94-95 -2.6077892385625994 2316 96-97 -3.194172782097919 2316 98-99 -3.0646234203505394 2316 100-101 -3.209233187622658 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1104.0 3 384.0 4 339.0 5 336.0 6 442.0 7 496.0 8 609.0 9 762.0 10 999.0 11 1454.0 12 2181.0 13 2991.0 14 3356.0 15 4385.0 16 6090.0 17 8522.0 18 7456.0 19 3837.0 20 3689.0 21 3829.0 22 4072.0 23 4340.0 24 4817.0 25 5473.0 26 6313.0 27 7377.0 28 8670.0 29 10577.0 30 12930.0 31 15902.0 32 20466.0 33 27402.0 34 38125.0 35 56159.0 36 81500.0 37 110760.0 38 95860.0 39 16731.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.67795967929452 19.49448410682145 15.024759083354144 20.80279713052989 2 14.661922811319814 21.88912673223336 40.25204888742701 23.196901569019822 3 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20.747298508657945 22-23 20.770646183147935 26.21214769556109 31.93305602171193 21.084150099579038 24-25 21.133452093645577 26.438060240580036 31.92890223626988 20.499585429504506 26-27 20.780767180984835 26.069051971074664 32.682033934495244 20.468146913445253 28-29 20.03461190752938 26.934435404020835 32.53235180162727 20.49860088682251 30-31 20.296278694142096 26.595860088129392 32.18057399476872 20.92728722295979 32-33 20.265766428950872 26.770104462358507 31.997699469035386 20.966429639655228 34-35 21.04420761893938 26.612907391814193 31.653571237926982 20.689313751319443 36-37 21.199957984423243 26.092792434441066 32.060037504068106 20.64721207706759 38-39 20.706880682997138 26.76670664477485 31.727837764072913 20.798574908155103 40-41 20.38805862206128 27.038056814013917 32.050997267265565 20.522887296659245 42-43 20.334936468659652 27.38993726936634 30.691603944642754 21.58352231733125 44-45 20.524695344019754 27.14249307345666 30.69633930677742 21.63647227574616 46-47 21.219416017922054 27.506341080385788 30.051985666489305 21.222257235202854 48-49 21.214821549569468 27.52345947509667 29.67194187371015 21.589777101623717 50-51 20.70799690737804 27.968684619715983 30.04363420963362 21.279684263272358 52-53 21.023584828497064 28.593322065973464 29.00306250629592 21.380030599233553 54-55 20.41433950112918 27.8444513514421 29.576232734060405 22.16497641336831 56-57 21.078406471781953 28.26772555194998 28.783966566592596 21.86990140967547 58-59 21.548561514983234 27.56716732747005 29.135014830408146 21.74925632713857 60-61 21.161181225343064 27.64452342699309 30.17526548984965 21.019029857814193 62-63 20.99115675133949 27.804875108807863 29.946420468879015 21.257547670973636 64-65 20.740741378508737 28.415405464510986 29.53408560619497 21.309767550785306 66-67 20.682686487226537 27.993814111643918 29.120344976936387 22.203154424193162 68-69 20.40874494429141 28.144054435030753 29.630305749278186 21.81689487139965 70-71 21.156534118066517 28.14661902461248 29.021434618342536 21.67541223897847 72-73 20.888333943295084 27.741491033562248 29.273514025532283 22.096660997610385 74-75 20.403901580420797 29.13561238399584 28.633491005129713 21.82699503045365 76-77 20.732898349518912 28.064465565886614 28.935093479032243 22.267542605562234 78-79 20.828668763283257 28.055537375692797 29.114236899303847 22.001556961720098 80-81 21.04214044517952 28.45927227620426 28.5234477293088 21.97513954930742 82-83 20.674350661655016 28.292427056022767 29.096283638292963 21.936938644029258 84-85 20.576222408036838 28.306751414205173 28.59213280826831 22.524893369489682 86-87 20.644094650914475 28.4440980952709 28.635690421244792 22.276116832569834 88-89 20.825382501050544 27.94014314942513 28.304647189710874 22.929827159813453 90-91 20.435483912637974 27.554984494368284 29.02123767752248 22.98829391547127 92-93 20.759462054888317 27.9077759164919 29.044385208662586 22.288376819957197 94-95 20.44855312941841 27.53428384943522 29.113588338927478 22.903574682218895 96-97 19.770884427788037 27.58704428339166 29.45081498525724 23.191256303563062 98-99 20.04456242011665 28.12979084773674 28.47671281957646 23.348933912570153 100-101 20.017140765305026 27.774327612567024 28.882620211460196 23.325911410667757 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 612.0 1 431.0 2 311.5 3 390.0 4 384.0 5 437.0 6 687.0 7 1241.5 8 1945.0 9 2187.5 10 1796.0 11 1475.0 12 1494.0 13 1566.0 14 1614.5 15 1628.5 16 1650.5 17 1681.0 18 1730.0 19 1804.5 20 1908.0 21 2172.0 22 2485.0 23 2811.5 24 3178.0 25 3754.5 26 4682.5 27 5538.5 28 6021.0 29 6911.5 30 8295.5 31 9479.5 32 10822.5 33 12146.0 34 13651.0 35 14878.0 36 16185.5 37 17487.0 38 17731.5 39 17373.5 40 16536.5 41 16389.5 42 16656.0 43 17155.0 44 17806.5 45 18101.5 46 18251.0 47 20767.5 48 29547.5 49 29080.0 50 19745.0 51 16106.0 52 14919.0 53 14807.5 54 14700.5 55 13910.0 56 12681.5 57 11545.0 58 10705.0 59 9563.5 60 8314.5 61 7219.0 62 6095.0 63 4802.5 64 3520.0 65 2593.5 66 1890.5 67 1544.5 68 1117.0 69 645.0 70 464.0 71 345.5 72 259.5 73 201.5 74 141.5 75 105.0 76 75.5 77 47.5 78 32.5 79 19.0 80 13.0 81 10.0 82 6.0 83 6.0 84 4.5 85 4.0 86 1.5 87 1.5 88 3.0 89 1.5 90 0.0 91 1.0 92 1.0 93 0.5 94 2.0 95 1.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024451688113552264 2 0.00964291925604878 3 0.013775598937212542 4 0.023590713179976477 5 0.05957946540344425 6 0.04442630657251045 7 0.01498096384421864 8 0.04838679126695905 9 0.03375021739617073 10-11 0.025484858033843205 12-13 0.03736631211718902 14-15 0.0191136435253824 16-17 0.0203190084323885 18-19 0.018424863578521775 20-21 0.014722671364145906 22-23 0.0073182869353941625 24-25 0.00266902229408493 26-27 8.60974933575784E-5 28-29 0.0020663398405818814 30-31 0.0 32-33 2.5829248007273517E-4 34-35 0.0 36-37 0.0 38-39 4.3048746678789194E-4 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 2.5829248007273517E-4 50-51 0.0 52-53 6.026824535030487E-4 54-55 9.470724269333622E-4 56-57 8.60974933575784E-5 58-59 7.748774402182055E-4 60-61 0.003099509760872822 62-63 0.001980242347224303 64-65 0.0013775598937212543 66-67 0.010417796696266985 68-69 0.014033891417285279 70-71 0.02712071040763719 72-73 0.0171334011781581 74-75 0.017305596164873257 76-77 0.030822902622013065 78-79 0.020491203419103654 80-81 0.05114191105440156 82-83 0.038399482037479955 84-85 0.019027546032024823 86-87 0.013259013977067071 88-89 0.014636573870788325 90-91 0.024365590620194683 92-93 0.03349192491609799 94-95 0.012053649070060974 96-97 0.019458033498812716 98-99 0.03495558230317683 100-101 0.04296264918543161 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 580737.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.71195715603688 #Duplication Level Percentage of deduplicated Percentage of total 1 76.65237852443735 39.638445141640254 2 12.797945695045257 13.23613638934934 3 4.356676061028309 6.758767374318824 4 1.9547353603733335 4.043327648280645 5 1.02831161167887 2.658800300309648 6 0.654307609262214 2.030131623422192 7 0.43781987711422365 1.5848365909174447 8 0.30794406769398325 1.2739512348037587 9 0.24719514962034841 1.1504650487712824 >10 1.4088872448244363 13.653895092618761 >50 0.10097225620171303 3.5780921385622477 >100 0.0437992221286203 4.010506059836012 >500 0.004680832899241845 1.646665792114075 >1k 0.004012142485064439 3.8185910000663013 >5k 3.343452070887032E-4 0.9173885649892146 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5306 0.9136665995106218 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3556 0.6123253727590975 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3097 0.5332878738568405 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2661 0.4582108596490322 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2404 0.4139567480632369 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1849 0.31838853043632487 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1473 0.25364321543142593 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1429 0.24606663601595904 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1238 0.2131773935533641 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1137 0.19578569989513328 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1101 0.1895866803733876 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1077 0.18545400069222384 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1064 0.18321546586492682 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 899 0.15480329305692594 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 879 0.1513593933226228 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 873 0.15032622340233187 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 727 0.12518575534191898 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 722 0.12432478040834319 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 688 0.11847015086002786 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 667 0.11485405613900956 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 645 0.11106576643127612 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 631 0.10865503661726392 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 595 0.10245601709551828 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 594 0.10228382210880312 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.7219498671515676E-4 0.0 12-13 0.0 0.0 0.0 1.7219498671515676E-4 0.0 14-15 0.0 0.0 0.0 1.7219498671515676E-4 0.0 16-17 0.0 0.0 0.0 1.7219498671515676E-4 0.0 18-19 0.0 0.0 0.0 2.5829248007273517E-4 0.0 20-21 0.0 0.0 0.0 5.165849601454703E-4 0.0 22-23 0.0 0.0 0.0 7.748774402182054E-4 0.0 24-25 0.0 0.0 0.0 0.001119267413648519 0.0 26-27 0.0 0.0 0.0 0.00266902229408493 0.0 28-29 0.0 0.0 0.0 0.0066295069885335366 0.0 30-31 0.0 0.0 0.0 0.015325353817648952 0.0 32-33 0.0 0.0 0.0 0.025484858033843205 0.0 34-35 0.0 0.0 0.0 0.03495558230317683 0.0 36-37 0.0 0.0 0.0 0.044770696545940764 0.0 38-39 0.0 0.0 0.0 0.05725483308278963 0.0 40-41 0.0 0.0 0.0 0.07266628439379616 0.0 42-43 0.0 0.0 0.0 0.09358797527968771 0.0 44-45 0.0 0.0 0.0 0.11605942104601566 0.0 46-47 0.0 0.0 0.0 0.13947793923927698 0.0 48-49 0.0 0.0 0.0 0.1597108501783079 0.0 50-51 0.0 0.0 0.0 0.18605668314572688 0.0 52-53 0.0 0.0 0.0 0.25476248284507447 0.0 54-55 0.0 0.0 0.0 0.34714509321775605 0.0 56-57 0.0 0.0 0.0 0.4273018595336615 0.0 58-59 0.0 0.0 0.0 0.5468912778073379 0.0 60-61 0.0 0.0 0.0 0.7060855430255004 0.0 62-63 0.0 0.0 0.0 0.8028591255594184 0.0 64-65 0.0 0.0 0.0 0.8645910282968021 0.0 66-67 0.0 0.0 0.0 1.0444486919207834 0.0 68-69 0.0 0.0 0.0 1.2454002414173715 0.0 70-71 0.0 0.0 0.0 1.3298618824011559 0.0 72-73 0.0 0.0 0.0 1.49930174932887 0.0 74-75 0.0 0.0 0.0 1.757852521881678 0.0 76-77 0.0 0.0 0.0 2.004694035337855 0.0 78-79 0.0 0.0 0.0 2.229666785481207 0.0 80-81 0.0 0.0 0.0 2.4717729368027177 0.0 82-83 0.0 0.0 0.0 2.694334957132058 0.0 84-85 0.0 0.0 0.0 3.0035971532724797 0.0 86-87 0.0 0.0 0.0 3.579158896367891 0.0 88-89 0.0 0.0 0.0 4.253560561837803 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGTG 55 0.0025705383 34.56746 1 GTCCTAT 95 3.4727684E-5 30.01911 1 GTACATG 4165 0.0 27.844852 1 TACATGG 4220 0.0 26.465988 2 CATATAG 145 7.8385165E-7 26.221329 3 ACATGGG 4085 0.0 25.944513 3 CATGGGG 1325 0.0 25.825535 4 GAGTACT 3525 0.0 24.13281 12-13 ATGGGAT 1030 0.0 23.53237 5 TAAGGTG 225 2.7885108E-9 23.23501 5 AGTACTT 3600 0.0 23.101997 12-13 GTATAGA 165 2.4130477E-6 23.044971 1 GTCTAGG 125 2.2443362E-4 22.814522 1 GTACTTT 3815 0.0 22.293533 14-15 CATGGGA 2705 0.0 21.96218 4 ATGGGGA 675 0.0 20.418644 5 GTGCATA 280 1.5588739E-9 20.370108 1 GTATAGG 140 4.8269847E-4 20.370108 1 AGGTGTG 235 1.124954E-7 20.220411 7 GTAAGGT 215 1.0712356E-6 19.89467 4 >>END_MODULE