FastQCFastQC Report
Thu 26 May 2016
SRR936905_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936905_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580737
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68781.1843571186268482No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51550.8876651565166331No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT48550.836006660502086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37360.6433204703678257No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT19520.336124614067986No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15160.26104759986017767No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14750.25398760540485626No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14650.2522656555377047No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14000.24107298140121947No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT12770.2198929980352552No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12650.21782665819467334No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12550.21610470832752177No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC11620.20009057456301219No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT11210.19303058010769075No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC10350.17822181125018724No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10160.1749501065025993No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9730.16754572207384755No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG9530.1641018223395444No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA9200.15841938777794423No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT9110.15686963289750783No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5820.10021748226822125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGA753.07873E-431.6629071
AGGTAAG1554.5884008E-827.5749972
CGATTCG700.00838249627.1466489
GTAAGGT1701.1239899E-725.1397424
GTATGGT1351.3424749E-524.6267031
GAGTACT49550.023.95871712-13
ACACCGT1201.7077778E-423.7533156
AGTACTT50650.023.06337712-13
TAGTAGC1903.2767275E-722.5031419
TAAGGTG2554.8748916E-1022.3560625
GTACTTT52950.022.1064114-15
GTACATG39700.022.0124991
GTAAGGG1302.9448708E-421.9166983
ACATGGG38500.020.9679243
TACATGG40800.020.8351922
GGGCATA1150.00372365220.6497231
AGTAAGG1150.003725510620.6479432
CCAATAC1856.5594086E-620.5345633
GGTGTGC3950.020.4458928
AGACTAG1404.8431958E-420.3599875
Produced by FastQC (version 0.11.5)