##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936905_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 580737 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.33003235543801 33.0 31.0 34.0 31.0 34.0 2 32.42783738594235 34.0 31.0 34.0 31.0 34.0 3 32.52246025309219 34.0 31.0 34.0 31.0 34.0 4 36.156850691448966 37.0 35.0 37.0 35.0 37.0 5 36.100041843381774 37.0 35.0 37.0 35.0 37.0 6 36.17698028539597 37.0 36.0 37.0 35.0 37.0 7 36.128317982150264 37.0 35.0 37.0 35.0 37.0 8 36.107730349538606 37.0 35.0 37.0 35.0 37.0 9 37.80020043496454 39.0 38.0 39.0 35.0 39.0 10-11 37.72209623289028 39.0 37.0 39.0 35.0 39.0 12-13 37.63301976626252 39.0 37.0 39.0 35.0 39.0 14-15 38.766053308123986 40.0 38.0 41.0 35.0 41.0 16-17 38.73102196002665 40.0 38.0 41.0 35.0 41.0 18-19 38.96140335470273 40.0 38.0 41.0 35.0 41.0 20-21 39.00462257441837 40.0 38.0 41.0 35.0 41.0 22-23 38.91771404267336 40.0 38.0 41.0 35.0 41.0 24-25 38.78300762651596 40.0 38.0 41.0 35.0 41.0 26-27 38.56760030788463 40.0 38.0 41.0 34.5 41.0 28-29 38.46395700635572 40.0 38.0 41.0 34.0 41.0 30-31 38.357134985372035 40.0 37.5 41.0 34.5 41.0 32-33 38.080822299939555 40.0 37.0 41.0 34.0 41.0 34-35 37.85088430735428 40.0 37.0 41.0 33.0 41.0 36-37 37.62528390648434 40.0 36.0 41.0 33.0 41.0 38-39 37.40013896135428 40.0 36.0 41.0 33.0 41.0 40-41 37.1666597788672 40.0 36.0 41.0 33.0 41.0 42-43 36.806294415544386 40.0 35.0 41.0 31.5 41.0 44-45 36.349958759300684 39.0 35.0 41.0 30.5 41.0 46-47 35.710041722845276 39.0 35.0 41.0 29.0 41.0 48-49 35.11480670251766 39.0 35.0 40.5 25.5 41.0 50-51 34.735432734611365 39.0 35.0 40.0 23.5 41.0 52-53 34.47443593227227 38.0 35.0 40.0 23.0 41.0 54-55 34.17452306293555 38.0 34.0 40.0 21.5 41.0 56-57 33.86343043408634 38.0 34.0 40.0 19.5 41.0 58-59 33.52256012618449 38.0 33.5 40.0 18.0 41.0 60-61 33.145596027117264 37.0 33.0 40.0 15.5 41.0 62-63 32.786195816695 36.5 33.0 39.5 13.0 41.0 64-65 32.406567000208355 36.0 32.0 39.0 9.0 40.0 66-67 31.99049828063306 35.5 32.0 39.0 7.0 40.0 68-69 31.67899238381574 35.0 31.5 38.5 4.0 40.0 70-71 31.202864635798996 35.0 31.0 37.5 2.0 40.0 72-73 30.689007244243093 35.0 30.5 37.0 2.0 39.0 74-75 30.188098054713237 34.5 30.0 36.5 2.0 39.0 76-77 28.829406426661293 33.0 28.5 35.0 2.0 37.5 78-79 29.51113843271567 34.0 30.0 35.5 2.0 37.5 80-81 29.23746119155487 34.0 29.0 35.0 2.0 37.0 82-83 28.927901098087432 34.0 29.0 35.0 2.0 37.0 84-85 28.589600800362298 34.0 29.0 35.0 2.0 36.0 86-87 28.281044603667407 34.0 29.0 35.0 2.0 36.0 88-89 27.879871611417904 34.0 28.0 35.0 2.0 35.5 90-91 27.42030385527356 33.0 27.0 35.0 2.0 35.0 92-93 26.973332162407424 33.0 26.0 35.0 2.0 35.0 94-95 26.399425213134343 33.0 25.0 35.0 2.0 35.0 96-97 25.710059803318885 32.0 23.5 34.0 2.0 35.0 98-99 24.95178109884509 32.0 18.0 34.0 2.0 35.0 100-101 21.620384098137365 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12817325785806588 1101 2 0.049113535886888826 1101 3 0.030058932732202948 1101 4 0.07124398483367145 1101 5 0.10684020151081342 1101 6 0.17158181201820355 1101 7 0.09677320909074183 1101 8 0.0352512170039887 1101 9 -0.00794204974170043 1101 10-11 0.08531859577190914 1101 12-13 0.11753802679903913 1101 14-15 0.07500871381520113 1101 16-17 0.27588288830212804 1101 18-19 0.099946226636483 1101 20-21 0.10792555195131115 1101 22-23 0.04043743104767117 1101 24-25 0.0802765970051098 1101 26-27 0.0864884799958503 1101 28-29 0.0886836350381941 1101 30-31 0.24034804206663551 1101 32-33 0.09962978126454658 1101 34-35 0.2529960199544732 1101 36-37 0.48490920807701343 1101 38-39 0.3373449780475326 1101 40-41 0.4519284931265872 1101 42-43 0.34339270987599235 1101 44-45 0.34719566119913736 1101 46-47 0.3043667709265492 1101 48-49 0.0742176305806197 1101 50-51 -0.03631333569861539 1101 52-53 -0.25228743755127425 1101 54-55 -0.1935157153129765 1101 56-57 -0.15769679453041618 1101 58-59 -0.05938459174536348 1101 60-61 0.1415459895692095 1101 62-63 0.08600387401946108 1101 64-65 -0.14346551712606725 1101 66-67 -0.18822914144819336 1101 68-69 -0.02873941029287508 1101 70-71 0.008236037529346873 1101 72-73 0.14572823351203468 1101 74-75 0.09738126260414504 1101 76-77 -0.012003132559026852 1101 78-79 0.13788368416400232 1101 80-81 0.2599168965848939 1101 82-83 0.0926238469770091 1101 84-85 -0.005072624104791146 1101 86-87 -0.1811084374974392 1101 88-89 0.022665313628568384 1101 90-91 0.2112889358319201 1101 92-93 0.2052496806734787 1101 94-95 0.08890050921788273 1101 96-97 -0.3064387084276632 1101 98-99 -0.49473401138900286 1101 100-101 -0.06450700687489075 1102 1 0.28482553462727367 1102 2 0.31172018249566236 1102 3 0.2770572174663357 1102 4 0.18133134631619185 1102 5 0.14666112998441605 1102 6 0.16762908125176068 1102 7 0.1765525846447673 1102 8 0.1086136770260282 1102 9 0.3716854978369497 1102 10-11 0.22508674683424346 1102 12-13 0.30177672193330096 1102 14-15 0.3800649384834429 1102 16-17 0.29568928440403397 1102 18-19 0.1940823589070817 1102 20-21 0.34994064554759063 1102 22-23 0.2370518716164085 1102 24-25 0.3730490650366818 1102 26-27 0.24689557223924652 1102 28-29 0.34759121695932294 1102 30-31 0.4375775996469784 1102 32-33 0.4272399866318679 1102 34-35 0.4090341088918379 1102 36-37 0.29726878578338756 1102 38-39 0.46262442886511934 1102 40-41 0.676777919543909 1102 42-43 0.48321103664621745 1102 44-45 0.5139973686598935 1102 46-47 0.6484996510679295 1102 48-49 0.7390039471075625 1102 50-51 0.8965961677662975 1102 52-53 0.8741720715816328 1102 54-55 0.9322190335947766 1102 56-57 0.9308958511974978 1102 58-59 0.8756991923950252 1102 60-61 0.7450454664409918 1102 62-63 0.4938803451433387 1102 64-65 0.6646254086900782 1102 66-67 0.46440629115003773 1102 68-69 0.2735748048568425 1102 70-71 0.47298295683379976 1102 72-73 0.5338289714666153 1102 74-75 0.3214250901558131 1102 76-77 0.3014370812266556 1102 78-79 0.5067884971294063 1102 80-81 0.6013142714661903 1102 82-83 0.4137855446999623 1102 84-85 0.6024455552975212 1102 86-87 0.7319705111913954 1102 88-89 0.6966607309139157 1102 90-91 0.6919025289886953 1102 92-93 0.5131984419839206 1102 94-95 0.6088462822861658 1102 96-97 0.7530195054286359 1102 98-99 0.9957911424367119 1102 100-101 1.3908505507946494 1103 1 0.11219345505563183 1103 2 0.022666202683765846 1103 3 0.12725975450929639 1103 4 0.13698434378243007 1103 5 0.028340957458937055 1103 6 0.15408287902631201 1103 7 0.022815079212350042 1103 8 -0.048436580040217336 1103 9 0.025359454197186437 1103 10-11 -0.041010643143870595 1103 12-13 0.033224492795845606 1103 14-15 0.17353967875223475 1103 16-17 0.10246423048982223 1103 18-19 0.03321300759961332 1103 20-21 0.037490357321800616 1103 22-23 0.023474714842890876 1103 24-25 0.04871305034249218 1103 26-27 0.15458439498242882 1103 28-29 0.16578039639730946 1103 30-31 0.14778870556482815 1103 32-33 0.2929761718835593 1103 34-35 0.2506739209505824 1103 36-37 0.47448869837266017 1103 38-39 0.42920171683670816 1103 40-41 0.4086920585974809 1103 42-43 0.43380265800855256 1103 44-45 0.49771343140537283 1103 46-47 0.6556909460750262 1103 48-49 0.5073921786393853 1103 50-51 0.8106767971891102 1103 52-53 0.7929645572113486 1103 54-55 0.7700233516517372 1103 56-57 0.8504622005287885 1103 58-59 0.871727375383557 1103 60-61 0.8697410458855401 1103 62-63 1.1279309630043173 1103 64-65 0.9478748982475267 1103 66-67 0.7964276436382924 1103 68-69 0.964528491933013 1103 70-71 0.7793438089653613 1103 72-73 0.915253577667368 1103 74-75 1.0856939068310894 1103 76-77 0.7575882214200078 1103 78-79 0.985829603129261 1103 80-81 0.9791852545498365 1103 82-83 0.9725592116044304 1103 84-85 0.8528452994612579 1103 86-87 0.6552206567392709 1103 88-89 0.7437864678781558 1103 90-91 0.9469331555415508 1103 92-93 0.9095437553423444 1103 94-95 0.833683924656583 1103 96-97 0.9511081133349641 1103 98-99 1.0688551507021913 1103 100-101 0.8493519816737596 1104 1 0.04723008601339984 1104 2 0.17192268644711817 1104 3 0.011310618731663169 1104 4 0.004848851150534017 1104 5 -0.06288437741675779 1104 6 -0.008627308906433484 1104 7 0.10179796241239814 1104 8 0.10313762908315738 1104 9 0.1944234787407666 1104 10-11 0.18150985175126522 1104 12-13 0.16738851901590124 1104 14-15 6.176683245655568E-4 1104 16-17 0.16622263381594138 1104 18-19 0.19599025449393537 1104 20-21 0.13302842166487494 1104 22-23 0.11470214531113498 1104 24-25 0.051789380205526925 1104 26-27 -0.17889353423683474 1104 28-29 -0.09451944098470477 1104 30-31 -0.07417017052697616 1104 32-33 -0.04211955478411511 1104 34-35 -0.03436857766632073 1104 36-37 -0.01216237708543133 1104 38-39 2.5906901361594237E-4 1104 40-41 -0.10128924871697365 1104 42-43 -0.1309762988893013 1104 44-45 -0.2525233705527441 1104 46-47 -0.30420779489296734 1104 48-49 -0.40114778011935215 1104 50-51 -0.20539011088982306 1104 52-53 -0.1799100928246986 1104 54-55 -0.17109049770031248 1104 56-57 -0.07767783534774964 1104 58-59 -0.09047442179660692 1104 60-61 -0.06693852963123703 1104 62-63 -0.018662022628298303 1104 64-65 0.07173070471537102 1104 66-67 -0.1399250194407209 1104 68-69 -0.14618375806279715 1104 70-71 -0.2961918244903572 1104 72-73 0.10170892478131677 1104 74-75 0.0032512003314018045 1104 76-77 -0.0707576016449245 1104 78-79 0.15574862943313406 1104 80-81 0.34022651257595626 1104 82-83 0.3434141295410207 1104 84-85 0.1477957596533841 1104 86-87 -0.0033423535375156632 1104 88-89 0.30262861108256445 1104 90-91 0.5281960434357309 1104 92-93 0.29605903640321785 1104 94-95 0.3758870630159876 1104 96-97 0.34151700535327123 1104 98-99 0.16310023444206578 1104 100-101 0.7266864717741797 1105 1 0.1584000732961499 1105 2 0.17321964439943827 1105 3 0.10195132605316815 1105 4 0.1864481971203773 1105 5 0.11836171152327069 1105 6 -0.05340011417096946 1105 7 0.13246571912932836 1105 8 0.04616736323200854 1105 9 0.09788337902135424 1105 10-11 -0.0025723864852693623 1105 12-13 0.09725942880415062 1105 14-15 0.15438540687398472 1105 16-17 0.14111867531860156 1105 18-19 0.15903452264630857 1105 20-21 0.13853670806548735 1105 22-23 0.17340841713736665 1105 24-25 0.12158287623895347 1105 26-27 0.15214867493170203 1105 28-29 0.0838450798978343 1105 30-31 -0.07265116529877247 1105 32-33 0.04399946255576026 1105 34-35 0.0052138725244361694 1105 36-37 -0.047086881011630055 1105 38-39 -0.08284717051483881 1105 40-41 -0.151696503607063 1105 42-43 -0.12848836009360554 1105 44-45 -0.043160545303948084 1105 46-47 -0.05542400838132977 1105 48-49 -0.21623286459824698 1105 50-51 0.03479186694582381 1105 52-53 -0.012912878508707593 1105 54-55 0.07102934727031851 1105 56-57 -0.04304350683889879 1105 58-59 -0.1547772942809118 1105 60-61 0.05468281920503415 1105 62-63 0.03772284585915742 1105 64-65 -0.4482106546176823 1105 66-67 -0.5406673638562332 1105 68-69 -0.6475866931240617 1105 70-71 -0.7404302023340783 1105 72-73 -0.41590346141945744 1105 74-75 -0.3843424127815034 1105 76-77 -0.495193399703016 1105 78-79 -0.44328950213996876 1105 80-81 -0.2732648780455733 1105 82-83 -0.24068879946440802 1105 84-85 -0.3783175046434337 1105 86-87 -0.36096463093511844 1105 88-89 -0.4653395554125197 1105 90-91 -0.33215135962044684 1105 92-93 -0.44718113800482584 1105 94-95 -0.4828732066705186 1105 96-97 -0.424828702069167 1105 98-99 -0.24084160935776566 1105 100-101 -0.04716594753182335 1106 1 0.006614359010434612 1106 2 0.0705603988830461 1106 3 0.058468023641289335 1106 4 0.11544479575983502 1106 5 0.16261805042246635 1106 6 0.0859010608444919 1106 7 0.010905731497913962 1106 8 0.08118591610770665 1106 9 0.0423407074072486 1106 10-11 0.008520687107797187 1106 12-13 0.07627148817334728 1106 14-15 0.17326682307682972 1106 16-17 0.10453472355607829 1106 18-19 -0.18653211309889883 1106 20-21 0.09217384473834045 1106 22-23 0.031034422084808 1106 24-25 0.1458175701165061 1106 26-27 0.22390578686738394 1106 28-29 0.28885435633567624 1106 30-31 0.3018077091601086 1106 32-33 0.2544393203170472 1106 34-35 0.4272917946023611 1106 36-37 0.40198934038601664 1106 38-39 0.3740367194047636 1106 40-41 0.43565661946034595 1106 42-43 0.4613771315576045 1106 44-45 0.5203444021295667 1106 46-47 0.9857981623051231 1106 48-49 0.7938745874734892 1106 50-51 0.744709925078169 1106 52-53 0.5637336892092861 1106 54-55 0.39452579130247756 1106 56-57 0.7788295605698821 1106 58-59 0.8108805597697923 1106 60-61 0.6758369626806058 1106 62-63 0.9227916461065249 1106 64-65 0.631875925468897 1106 66-67 0.742321968240244 1106 68-69 0.7407868381066045 1106 70-71 0.6232061613022886 1106 72-73 0.54815034192006 1106 74-75 0.6078112668721793 1106 76-77 0.6331499831046514 1106 78-79 0.6989298856151329 1106 80-81 0.682478783055231 1106 82-83 0.5879290112962572 1106 84-85 0.6044824335136454 1106 86-87 0.41274826331348535 1106 88-89 0.5431251941236575 1106 90-91 0.8614169768050317 1106 92-93 0.8993517933495347 1106 94-95 0.8957987166442578 1106 96-97 0.9642212360879121 1106 98-99 0.9094753356020355 1106 100-101 1.0136913499326212 1107 1 0.10512353332236302 1107 2 -0.03056553939885731 1107 3 0.024053928562061344 1107 4 0.04461146242650216 1107 5 0.16529736651921212 1107 6 0.07396055486729125 1107 7 0.12207128612677565 1107 8 0.10451785939377345 1107 9 0.16295858341503333 1107 10-11 0.23176575244001896 1107 12-13 0.132335569352108 1107 14-15 0.18891701768345115 1107 16-17 0.2512837505229939 1107 18-19 0.028843547173551087 1107 20-21 0.14515375855540924 1107 22-23 0.08356257054185079 1107 24-25 0.19272123984127632 1107 26-27 0.2554720226193936 1107 28-29 0.1510172698747354 1107 30-31 0.006728476466193456 1107 32-33 0.05688406593839801 1107 34-35 0.25308412154146964 1107 36-37 0.4294827376615231 1107 38-39 0.2593421822905029 1107 40-41 0.24557460667191577 1107 42-43 0.18314179655620677 1107 44-45 0.14226197232694915 1107 46-47 -0.0474604559878955 1107 48-49 -0.004184559037490487 1107 50-51 0.175509897277216 1107 52-53 0.17154227970137015 1107 54-55 -0.14898095042673987 1107 56-57 0.03846432565189417 1107 58-59 -0.07664897873451793 1107 60-61 -0.0013054476613447719 1107 62-63 -0.09025568500273096 1107 64-65 -0.0692569630493125 1107 66-67 -0.12103464838472533 1107 68-69 -0.09616061810690013 1107 70-71 -0.14127118092734747 1107 72-73 -0.34193167086837306 1107 74-75 -0.12355167446783 1107 76-77 -0.37278030602518086 1107 78-79 -0.40269473212186924 1107 80-81 -0.39986414160420836 1107 82-83 -0.4826019867576754 1107 84-85 -0.5215288258691722 1107 86-87 -0.5261365712708859 1107 88-89 -0.4360831739608564 1107 90-91 -0.3146047079322507 1107 92-93 -0.045294787134199765 1107 94-95 0.27923282840406216 1107 96-97 0.13616486967145747 1107 98-99 -0.02685702469821294 1107 100-101 0.17157118034685226 1108 1 0.18076733642316611 1108 2 0.08404742520374953 1108 3 0.0698199930271457 1108 4 0.07664431663594229 1108 5 0.16045965953204444 1108 6 0.09590354979975046 1108 7 0.10910668352330077 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74-75 -0.3525496956946661 2316 76-77 -0.20794850174383228 2316 78-79 -0.32080050654172965 2316 80-81 -0.5249855663208827 2316 82-83 -0.2065451420014206 2316 84-85 -0.3019569604257484 2316 86-87 -0.33581457091111133 2316 88-89 -0.6903195474093202 2316 90-91 -0.18261154369407606 2316 92-93 -0.4947101496094639 2316 94-95 -0.6597939749778448 2316 96-97 -0.22248776569461626 2316 98-99 -0.48961111716088723 2316 100-101 -0.6160434985522301 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 7.0 5 11.0 6 20.0 7 65.0 8 184.0 9 337.0 10 613.0 11 1148.0 12 1937.0 13 2879.0 14 3633.0 15 5229.0 16 7556.0 17 11286.0 18 9564.0 19 4700.0 20 3705.0 21 3602.0 22 3614.0 23 3819.0 24 4223.0 25 4769.0 26 5524.0 27 6845.0 28 8444.0 29 10499.0 30 13122.0 31 16503.0 32 21057.0 33 28331.0 34 39939.0 35 59069.0 36 88271.0 37 123343.0 38 81808.0 39 5075.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.16670771848899 19.890383022114587 15.50708186996505 19.435827389431363 2 15.444371160863582 22.822573562460285 39.273493749752454 22.45956152692368 3 19.840995149267222 27.72235969121995 31.34465343175999 21.091991727752838 4 12.102552446288078 17.916199587765202 38.98597816223178 30.995269803714937 5 11.995274969564536 39.559215273006544 33.68151159647138 14.763998160957541 6 28.504985905840336 38.77951637316031 17.338313212349135 15.377184508650215 7 25.851288965573055 36.08690336589541 21.19685847466237 16.86494919386917 8 26.34393882256512 32.87047320904299 20.126150047267526 20.659437921124365 9 27.305820018355988 16.98892958430408 21.329448614433037 34.3758017829069 10-11 24.44366182629781 27.429857131048163 26.864047172930672 21.262433869723356 12-13 27.260360541863182 25.575174304375302 27.6161153844167 19.548349769344817 14-15 23.5102981211805 25.989389344918614 28.72780621864975 21.77250631525114 16-17 20.13183248182912 29.339701103942062 28.961560913115576 21.56690550111324 18-19 19.697280170818015 27.29231060638673 33.47731753725881 19.533091685536434 20-21 21.600355412594592 25.375494955947953 33.339905481602145 19.684244149855314 22-23 20.768109490817075 25.7150335486104 33.232023102176676 20.28483385839585 24-25 21.17632673867519 25.885095200044088 33.53631654359087 19.402261517689848 26-27 21.0008162478087 25.414581561065326 34.30970232785609 19.27489986326988 28-29 19.939266723605904 26.481740412166634 34.10801121335684 19.47098165087062 30-31 20.368421958947515 25.916879046700647 33.55532786885246 20.15937112549938 32-33 20.509387532997998 26.371020652055993 33.25475231481082 19.86483950013518 34-35 21.111019273784862 25.84793116333211 33.206597823110975 19.83445173977205 36-37 21.41689786232277 25.430575001248545 33.4299447024766 19.722582433952095 38-39 20.72373604515493 26.213280405739948 33.39320695578795 19.669776593317174 40-41 20.547794237931978 26.46010445491787 33.258800576516755 19.733300730633392 42-43 20.411218847774464 27.185025235175303 31.938381745953848 20.465374171096382 44-45 20.94399013667116 26.47403213502842 31.76549797929183 20.816479749008586 46-47 21.768583314965838 27.319814855631474 30.672629215340535 20.238972614062156 48-49 21.90265696261276 27.312411585972228 30.215189949708936 20.569741501706073 50-51 21.172464267263646 27.846995006027207 30.503271913208195 20.47726881350095 52-53 21.556573801910332 28.61570728229818 29.331091354606304 20.496627561185186 54-55 20.777471929025488 27.888096666956542 29.754759430392337 21.57967197362564 56-57 21.542778520161455 28.158192012563887 29.17177533786111 21.127254129413547 58-59 22.267825194537284 27.480339637391797 29.157949295464213 21.093885872606705 60-61 21.38526960718895 27.79846553744504 30.520926876685934 20.29533797868008 62-63 21.433048737226752 27.558265283981914 30.46481352009451 20.54387245869683 64-65 21.132285354644186 28.45995691681432 29.83846388296251 20.56929384557898 66-67 21.29089194925287 27.78435483384921 29.458992892474463 21.465760324423456 68-69 21.059057209546204 28.016809775912705 29.766665776987207 21.157467237553888 70-71 21.79781568966779 28.296332648852957 29.207767776041464 20.698083885437786 72-73 21.650161777190018 27.61034685236174 29.47926514067469 21.260226229773547 74-75 21.125828042642368 29.279777248565185 28.731852800837554 20.862541907954892 76-77 21.472112160926546 28.155602277795282 28.805638352645 21.566647208633167 78-79 21.368904838245854 28.300130698245475 28.82843401460583 21.50253044890284 80-81 21.76494138817259 28.68789578588925 28.367693970063414 21.179468855874745 82-83 21.435681503104203 28.570518285069706 28.730162830547734 21.26363738127836 84-85 21.07085843982965 28.596780054176644 28.567676896397927 21.76468460959578 86-87 21.01895691750551 28.68705028228303 28.365720327563686 21.928272472647777 88-89 21.139726140621555 28.574946275071632 28.049147977738592 22.236179606568218 90-91 21.083921382250736 27.76744602520259 28.634021772446594 22.51461082010008 92-93 21.249998923780407 28.236299939817798 28.6285604572814 21.885140679120394 94-95 21.09130294780598 28.05650406294071 28.61639606224504 22.235796927008266 96-97 20.594239447036507 27.92153952254551 28.991838656990264 22.492382373427724 98-99 20.88468800610272 28.595487188847724 27.77384112431971 22.745983680729847 100-101 20.295814531744657 28.820004976447972 27.915634570299215 22.968545921508156 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1097.0 1 761.5 2 483.0 3 522.0 4 446.0 5 461.0 6 749.5 7 1460.5 8 2284.0 9 2534.5 10 2161.0 11 1778.0 12 1743.5 13 1801.0 14 1854.5 15 1936.0 16 1997.0 17 2075.0 18 2158.5 19 2242.5 20 2367.0 21 2518.5 22 2789.5 23 3143.0 24 3480.5 25 4026.0 26 4763.0 27 5485.5 28 6005.5 29 6939.5 30 8330.5 31 9360.5 32 10643.5 33 12013.5 34 13190.5 35 14261.5 36 15469.0 37 16796.0 38 17412.0 39 17014.0 40 16266.5 41 16175.5 42 16245.5 43 16637.5 44 17309.5 45 17522.5 46 17505.0 47 20502.0 48 30308.0 49 29244.0 50 19284.0 51 15945.5 52 14643.0 53 14504.5 54 14332.5 55 13644.0 56 12546.5 57 11442.5 58 10720.5 59 9861.5 60 8476.5 61 7076.5 62 5930.5 63 4850.5 64 3678.0 65 2739.5 66 1994.5 67 1622.0 68 1147.0 69 649.5 70 486.5 71 352.5 72 265.5 73 223.0 74 185.5 75 144.5 76 103.5 77 55.5 78 32.0 79 27.0 80 18.5 81 9.0 82 7.0 83 7.5 84 4.5 85 2.0 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02927314774157665 2 0.006887799468606271 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.01119267413648519 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.443899734303136E-4 20-21 6.026824535030487E-4 22-23 0.0021524373339394595 24-25 0.01119267413648519 26-27 0.00533804458816986 28-29 1.721949867151568E-4 30-31 0.0029273147741576654 32-33 0.0034438997343031355 34-35 0.0 36-37 0.010331699202909408 38-39 0.011967551576703397 40-41 0.0013775598937212543 42-43 0.0 44-45 0.0 46-47 1.721949867151568E-4 48-49 0.0030134122675152435 50-51 0.006371214508460801 52-53 0.0 54-55 0.0037021922143758708 56-57 0.004304874667878919 58-59 0.0 60-61 0.0021524373339394595 62-63 0.00533804458816986 64-65 0.0 66-67 0.0024968273073697734 68-69 6.026824535030487E-4 70-71 7.748774402182055E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0017219498671515677 80-81 0.0016358523737939894 82-83 0.012398039043491288 84-85 0.007576579415466898 86-87 0.004390972161236498 88-89 1.721949867151568E-4 90-91 5.165849601454703E-4 92-93 8.60974933575784E-5 94-95 0.0 96-97 0.001980242347224303 98-99 0.0021524373339394595 100-101 2.5829248007273517E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 580737.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.22658045897118 #Duplication Level Percentage of deduplicated Percentage of total 1 75.19486971504746 36.26391434414592 2 13.111715118254455 12.646663682112147 3 4.645532341272441 6.721144176933843 4 2.220478704441373 4.283443795886958 5 1.2291227399585873 2.9638193356281963 6 0.7559194551260282 2.1873246253882233 7 0.4751642085632438 1.6040881454849047 8 0.3625526460301035 1.3987739483506947 9 0.2675861112595349 1.1614286811925056 >10 1.548279321726463 13.89195331528415 >50 0.11686358606883393 3.8135311186803973 >100 0.06256696545874885 5.502056040502025 >500 0.003595802612571771 1.221349618420819 >1k 0.00503412365760048 4.2538273661596895 >5k 7.191605225143542E-4 2.086681805829578 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6878 1.1843571186268482 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5155 0.8876651565166331 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4855 0.836006660502086 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3736 0.6433204703678257 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1952 0.336124614067986 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1516 0.26104759986017767 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1475 0.25398760540485626 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1465 0.2522656555377047 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1400 0.24107298140121947 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1277 0.2198929980352552 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1265 0.21782665819467334 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1255 0.21610470832752177 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1162 0.20009057456301219 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1121 0.19303058010769075 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1035 0.17822181125018724 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1016 0.1749501065025993 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 973 0.16754572207384755 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 953 0.1641018223395444 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 920 0.15841938777794423 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 911 0.15686963289750783 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 582 0.10021748226822125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 8.609749335757838E-5 0.0 20-21 0.0 0.0 0.0 4.3048746678789194E-4 0.0 22-23 0.0 0.0 0.0 7.748774402182054E-4 0.0 24-25 0.0 0.0 0.0 0.0012914624003636758 0.0 26-27 0.0 0.0 0.0 0.002841217280800087 0.0 28-29 0.0 0.0 0.0 0.007146091948679006 0.0 30-31 0.0 0.0 0.0 0.015841938777794426 0.0 32-33 0.0 0.0 0.0 0.026518027954134143 0.0 34-35 0.0 0.0 0.0 0.035988752223467764 0.0 36-37 0.0 0.0 0.0 0.046664841399807484 0.0 38-39 0.0 0.0 0.0 0.059665562896801826 0.0 40-41 0.0 0.0 0.0 0.07567969666131141 0.0 42-43 0.0 0.0 0.0 0.09729016749406358 0.0 44-45 0.0 0.0 0.0 0.12010600323382184 0.0 46-47 0.0 0.0 0.0 0.14361061892044075 0.0 48-49 0.0 0.0 0.0 0.16358523737939895 0.0 50-51 0.0 0.0 0.0 0.19027546032024822 0.0 52-53 0.0 0.0 0.0 0.25889516252623823 0.0 54-55 0.0 0.0 0.0 0.3524831378059259 0.0 56-57 0.0 0.0 0.0 0.43307039158861926 0.0 58-59 0.0 0.0 0.0 0.553004199835726 0.0 60-61 0.0 0.0 0.0 0.711681880093743 0.0 62-63 0.0 0.0 0.0 0.8089720475878065 0.0 64-65 0.0 0.0 0.0 0.8707039503251902 0.0 66-67 0.0 0.0 0.0 1.0513364913893897 0.0 68-69 0.0 0.0 0.0 1.2524602358726928 0.0 70-71 0.0 0.0 0.0 1.336749681869762 0.0 72-73 0.0 0.0 0.0 1.508514181118131 0.0 74-75 0.0 0.0 0.0 1.7648264188436418 0.0 76-77 0.0 0.0 0.0 2.0120123222732493 0.0 78-79 0.0 0.0 0.0 2.2368989749232444 0.0 80-81 0.0 0.0 0.0 2.4825351234724153 0.0 82-83 0.0 0.0 0.0 2.7037195839080344 0.0 84-85 0.0 0.0 0.0 3.0140149499687467 0.0 86-87 0.0 0.0 0.0 3.5962062000526913 0.0 88-89 0.0 0.0 0.0 4.274568350217052 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTAGA 75 3.07873E-4 31.662907 1 AGGTAAG 155 4.5884008E-8 27.574997 2 CGATTCG 70 0.008382496 27.146648 9 GTAAGGT 170 1.1239899E-7 25.139742 4 GTATGGT 135 1.3424749E-5 24.626703 1 GAGTACT 4955 0.0 23.958717 12-13 ACACCGT 120 1.7077778E-4 23.753315 6 AGTACTT 5065 0.0 23.063377 12-13 TAGTAGC 190 3.2767275E-7 22.503141 9 TAAGGTG 255 4.8748916E-10 22.356062 5 GTACTTT 5295 0.0 22.10641 14-15 GTACATG 3970 0.0 22.012499 1 GTAAGGG 130 2.9448708E-4 21.916698 3 ACATGGG 3850 0.0 20.967924 3 TACATGG 4080 0.0 20.835192 2 GGGCATA 115 0.003723652 20.649723 1 AGTAAGG 115 0.0037255106 20.647943 2 CCAATAC 185 6.5594086E-6 20.534563 3 GGTGTGC 395 0.0 20.445892 8 AGACTAG 140 4.8431958E-4 20.359987 5 >>END_MODULE