FastQCFastQC Report
Thu 26 May 2016
SRR936904_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936904_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587121
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70881.2072468877795208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53340.9085009733939001No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT48650.828619654210972No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39660.6754995988901777No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT19180.32667882770331835No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15520.26434074066504176No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15070.25667622176689303No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14950.25463235006072005No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14240.24253944246586306No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12990.22124911219322763No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT12660.21562846500125188No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12550.21375491593725995No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC11560.19689297436133266No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT11310.19263490830680557No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC11220.19110200452717585No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10250.17458070823561073No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9850.16776780254836737No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA9480.16146586478766728No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG9470.1612955421454862No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT8550.1456258590648265No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA6180.10525939286790968No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC5950.10134197209774476No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG651.3276594E-436.5372161
GCGACGA700.00837226227.1534989
TAACGCG350.00838606427.14655976-77
ACGATGG908.944091E-426.3879893
GTATAGG2600.025.5760521
CATGGGT3350.025.5215194
CATATAG1351.3416275E-524.628793
GTATAAT2351.7644197E-1024.2544941
GTACATG39100.024.1743681
AGACCCG1401.7780263E-523.7491915
TACATGG40400.023.2789122
GAGTACT56950.023.27556812-13
ACATGGG39000.023.0184483
AGTACTT58100.022.93752312-13
GTACTTT60550.021.9645714-15
CGTTTAG550.002877971621.59017490-91
TACCTGG3550.021.407722
GGTGTGC4500.021.1193878
TAATACC1353.7937667E-421.1103924
AGGTAAG1353.7937667E-421.1103922
Produced by FastQC (version 0.11.5)