##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936903_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 184272 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81229920986368 33.0 31.0 34.0 30.0 34.0 2 31.847459190761484 33.0 31.0 34.0 30.0 34.0 3 31.79457541026309 33.0 31.0 34.0 30.0 34.0 4 35.36536207345663 37.0 35.0 37.0 33.0 37.0 5 35.436620864808546 37.0 35.0 37.0 33.0 37.0 6 35.6053334201615 37.0 35.0 37.0 33.0 37.0 7 35.58397911782582 37.0 35.0 37.0 33.0 37.0 8 35.57949118694104 37.0 35.0 37.0 33.0 37.0 9 37.108470087696446 39.0 37.0 39.0 33.0 39.0 10-11 36.99488256490405 39.0 37.0 39.0 33.0 39.0 12-13 36.93936680559173 39.0 37.0 39.0 33.0 39.0 14-15 37.8185616914127 40.0 37.0 41.0 33.0 41.0 16-17 37.82586882434662 40.0 37.5 41.0 33.0 41.0 18-19 37.89342385169749 40.0 37.0 41.0 33.0 41.0 20-21 37.83326821220804 40.0 37.0 41.0 33.0 41.0 22-23 37.67812527133802 40.0 37.0 41.0 32.5 41.0 24-25 37.376915646435705 39.5 36.5 41.0 32.0 41.0 26-27 37.2451267691239 39.0 36.0 41.0 32.0 41.0 28-29 37.3031225579578 39.0 36.0 41.0 32.0 41.0 30-31 37.11186723973257 39.0 36.0 41.0 32.0 41.0 32-33 36.697398411044546 39.0 35.0 41.0 31.0 41.0 34-35 36.33295074672224 38.0 35.0 40.5 31.0 41.0 36-37 35.971843253451425 38.0 35.0 40.0 30.0 41.0 38-39 35.62643809151689 38.0 35.0 40.0 30.0 41.0 40-41 35.43605648172267 38.0 35.0 40.0 29.5 41.0 42-43 34.9761602413823 38.0 35.0 40.0 26.5 41.0 44-45 34.392748220022575 38.0 34.5 40.0 23.5 41.0 46-47 33.55497851002865 38.0 33.5 40.0 16.5 41.0 48-49 32.77204621429192 37.0 33.0 40.0 4.5 41.0 50-51 31.89464758617696 36.5 32.0 39.5 2.0 40.5 52-53 32.10952287922202 37.0 32.5 39.5 2.0 40.5 54-55 32.337349136059736 37.0 33.0 40.0 2.0 41.0 56-57 32.056335200138925 37.0 32.5 40.0 2.0 41.0 58-59 31.7818577971694 36.0 32.0 40.0 2.0 41.0 60-61 31.272526482590955 35.5 31.0 39.0 2.0 41.0 62-63 31.04456727012243 35.0 31.0 39.0 2.0 41.0 64-65 30.72514543717982 35.0 30.5 39.0 2.0 40.5 66-67 30.174369953112787 35.0 30.0 38.0 2.0 40.0 68-69 29.67447306156117 35.0 29.0 37.0 2.0 39.0 70-71 29.156165885213163 35.0 29.0 37.0 2.0 39.0 72-73 28.77680548319875 34.5 28.5 36.0 2.0 38.5 74-75 28.43554636624121 34.0 28.0 35.5 2.0 37.0 76-77 28.063058956325435 34.0 27.0 35.0 2.0 37.0 78-79 27.61706336285491 34.0 26.0 35.0 2.0 36.5 80-81 27.138499066597205 34.0 26.0 35.0 2.0 36.0 82-83 26.691013827385603 33.5 24.5 35.0 2.0 36.0 84-85 26.343361986628462 33.0 24.0 35.0 2.0 35.0 86-87 26.223552682990363 33.5 24.0 35.0 2.0 35.0 88-89 25.72813558218286 33.0 20.5 35.0 2.0 35.0 90-91 25.27325638187028 33.0 18.5 35.0 2.0 35.0 92-93 24.913733502648256 33.0 14.5 35.0 2.0 35.0 94-95 24.430371950160634 32.0 4.5 35.0 2.0 35.0 96-97 23.947922093427106 32.0 2.0 35.0 2.0 35.0 98-99 23.439144308413653 32.0 2.0 35.0 2.0 35.0 100-101 21.452594534166884 29.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.43244582798227427 1101 2 0.27239682812215804 1101 3 0.20661729210715052 1101 4 -0.1609732082067552 1101 5 0.1366359371800101 1101 6 -0.10538494412943322 1101 7 -0.02679222504821155 1101 8 -0.04868408307130778 1101 9 0.2138966637392059 1101 10-11 0.30027337498884066 1101 12-13 0.4863700303047267 1101 14-15 0.8643537770186427 1101 16-17 0.8613199357859358 1101 18-19 0.27399098715456205 1101 20-21 0.6203485804308357 1101 22-23 1.048040815603116 1101 24-25 0.6707738516956354 1101 26-27 0.8050655714858053 1101 28-29 0.6417707440511222 1101 30-31 0.14761183831476643 1101 32-33 0.6497148189482189 1101 34-35 0.725220264129014 1101 36-37 0.5636289820431699 1101 38-39 1.2175614691480732 1101 40-41 0.9632191829416215 1101 42-43 0.8389530170260144 1101 44-45 0.647904817552508 1101 46-47 -0.19894418651332302 1101 48-49 0.041601601535020905 1101 50-51 0.4072426200881978 1101 52-53 0.3766872738641567 1101 54-55 0.24884123295105098 1101 56-57 0.4071737513609719 1101 58-59 0.7852723677443016 1101 60-61 0.9885782935030427 1101 62-63 0.9608195539375686 1101 64-65 0.5024841088293357 1101 66-67 0.3861292115125394 1101 68-69 0.2878614839577587 1101 70-71 0.106200511816116 1101 72-73 0.3289527331123452 1101 74-75 0.6952572553836376 1101 76-77 0.2233555999447958 1101 78-79 0.3814756665900596 1101 80-81 -0.0389103188393598 1101 82-83 0.3017117851217357 1101 84-85 0.37243663272378313 1101 86-87 0.30807957884792003 1101 88-89 0.23710619485262185 1101 90-91 -0.1791643168591861 1101 92-93 0.19115510207341302 1101 94-95 0.0808038204145376 1101 96-97 0.014326181750284661 1101 98-99 0.2982680521996812 1101 100-101 0.26901044405247987 1102 1 0.7037520527420575 1102 2 0.7855565551796708 1102 3 0.9850604058796044 1102 4 0.881743683591047 1102 5 0.6927910687772822 1102 6 0.6108802000009064 1102 7 0.7556893234826347 1102 8 0.7272872301162678 1102 9 1.0920821532253484 1102 10-11 1.0671582099700387 1102 12-13 0.9136433905609493 1102 14-15 1.4483775898580902 1102 16-17 0.9598786323463031 1102 18-19 0.9904476087950016 1102 20-21 0.7697010383057759 1102 22-23 0.9124225173159743 1102 24-25 0.9224101140546352 1102 26-27 1.265934531240724 1102 28-29 0.8510904766788912 1102 30-31 0.6905550217154044 1102 32-33 0.8359005869470977 1102 34-35 0.6394035255132238 1102 36-37 0.8387821640512456 1102 38-39 0.954506184368789 1102 40-41 0.8787253798501311 1102 42-43 0.6052286053841769 1102 44-45 0.8375719948258649 1102 46-47 0.38695958733433145 1102 48-49 0.4824872720544491 1102 50-51 0.3480412553757617 1102 52-53 0.7489891816764356 1102 54-55 0.7603124765104425 1102 56-57 1.0852130216575802 1102 58-59 1.175677603783857 1102 60-61 1.0160292056322753 1102 62-63 1.097551903192553 1102 64-65 1.3616611036768482 1102 66-67 1.1087827416615497 1102 68-69 1.0351111706309233 1102 70-71 0.9061935489975674 1102 72-73 1.3359503657540373 1102 74-75 1.2430047835830536 1102 76-77 1.025002306531622 1102 78-79 1.5935805266374068 1102 80-81 1.5284549506217182 1102 82-83 1.6913337040745304 1102 84-85 1.5952816403828827 1102 86-87 1.340334835775927 1102 88-89 2.0496671195149254 1102 90-91 2.1409512659287273 1102 92-93 1.815493634311263 1102 94-95 1.8849919557717314 1102 96-97 1.8260167540939705 1102 98-99 2.103866158313039 1102 100-101 1.8098299677956895 1103 1 0.3673497129130503 1103 2 0.6068503837075916 1103 3 0.275981453956863 1103 4 0.600721912105115 1103 5 0.1275285302704816 1103 6 0.313555773708984 1103 7 0.27647434303889895 1103 8 0.31826712004738766 1103 9 0.35070940738754075 1103 10-11 0.5181117941311939 1103 12-13 0.38249182086796907 1103 14-15 0.4556462894531137 1103 16-17 0.45269646488930704 1103 18-19 0.362258899508376 1103 20-21 0.39209209434399384 1103 22-23 0.28925267203543825 1103 24-25 0.7510937871708236 1103 26-27 0.5559729515100074 1103 28-29 0.6562720211044919 1103 30-31 0.4144655820975345 1103 32-33 0.7022084336813776 1103 34-35 1.0336958656883652 1103 36-37 0.6989462289849584 1103 38-39 0.7937813911806089 1103 40-41 0.9783757900124712 1103 42-43 1.3083541462100357 1103 44-45 1.092789882749443 1103 46-47 0.7353379750675302 1103 48-49 1.3258467957815583 1103 50-51 0.7608687795182334 1103 52-53 0.937179275477277 1103 54-55 0.5974936055817821 1103 56-57 1.2479453604482131 1103 58-59 1.4376449984742443 1103 60-61 1.232411470005264 1103 62-63 1.388178742000477 1103 64-65 1.1655100531766927 1103 66-67 1.0500793392178807 1103 68-69 1.1141150126578516 1103 70-71 0.5089764225568096 1103 72-73 1.151879201723716 1103 74-75 1.2114724724827113 1103 76-77 0.7269683167078007 1103 78-79 1.091971028869935 1103 80-81 1.023228409484343 1103 82-83 1.121865703659175 1103 84-85 1.194153261302894 1103 86-87 1.4949158471635542 1103 88-89 1.244970155062326 1103 90-91 1.0971833105100828 1103 92-93 1.1405602640575516 1103 94-95 1.1983767468831488 1103 96-97 1.522358175297807 1103 98-99 1.8537190536783754 1103 100-101 2.047256848139046 1104 1 0.22964145041730077 1104 2 0.5339314631178063 1104 3 0.5809488429092902 1104 4 0.45185479826010067 1104 5 0.614530477660054 1104 6 0.48365870715771564 1104 7 0.4329764604386668 1104 8 0.45526443079512546 1104 9 0.5937312614260577 1104 10-11 0.22428780105524737 1104 12-13 0.2560815089765285 1104 14-15 0.4253381073258211 1104 16-17 0.6213311284236696 1104 18-19 0.6646606799788444 1104 20-21 0.6854524232364554 1104 22-23 0.8177989302654822 1104 24-25 1.0500799081562704 1104 26-27 1.1211332247627652 1104 28-29 0.877764401556405 1104 30-31 0.6876410635355086 1104 32-33 0.4637304886410405 1104 34-35 0.5059378365702543 1104 36-37 0.8318014730268786 1104 38-39 0.3929127936102006 1104 40-41 0.6869164842723023 1104 42-43 0.331444622547707 1104 44-45 0.019438208921741307 1104 46-47 0.060212012964349526 1104 48-49 -0.13157337670151037 1104 50-51 -0.15072591616350905 1104 52-53 -0.36942639359013896 1104 54-55 -0.693656590702723 1104 56-57 -0.818482261572747 1104 58-59 -0.576187649666462 1104 60-61 -0.3042180004902413 1104 62-63 -0.4857200555637391 1104 64-65 -0.1844018506284435 1104 66-67 -0.3039746312930802 1104 68-69 -0.3959465001238236 1104 70-71 -0.37132094073953326 1104 72-73 -0.2539660580827814 1104 74-75 -0.07437649663725665 1104 76-77 -0.01260696690012253 1104 78-79 0.20091665651668578 1104 80-81 -0.16591566643294087 1104 82-83 -0.6111255485432281 1104 84-85 -0.3235277995607575 1104 86-87 -0.07512835548860153 1104 88-89 0.3890151613990689 1104 90-91 0.206515324976408 1104 92-93 -0.19276791745152266 1104 94-95 -0.5068873871467119 1104 96-97 -0.5800961537801221 1104 98-99 -0.7397247347671154 1104 100-101 -0.5729712746392259 1105 1 0.484004734192343 1105 2 0.3526421641814501 1105 3 0.4154085008983621 1105 4 0.5188760123401437 1105 5 0.31929630007293497 1105 6 0.2010295715874051 1105 7 0.3614009770984765 1105 8 0.2195883421778575 1105 9 0.5198639522646289 1105 10-11 0.5577484580453174 1105 12-13 0.5648903471971352 1105 14-15 0.6607056809286931 1105 16-17 0.7628660640762561 1105 18-19 0.47565850568254575 1105 20-21 0.22263582806692028 1105 22-23 0.04417549487733652 1105 24-25 0.4205182933005389 1105 26-27 0.8329214365509827 1105 28-29 0.8482938720858826 1105 30-31 0.8407820402058945 1105 32-33 0.8657255524008107 1105 34-35 1.1299608723579908 1105 36-37 0.8581280875887884 1105 38-39 0.5327288598968565 1105 40-41 1.046850667735903 1105 42-43 1.5178758383906938 1105 44-45 0.9344496680508456 1105 46-47 0.9401115252168069 1105 48-49 0.42206416223014287 1105 50-51 0.6120407289560745 1105 52-53 0.8582870694174431 1105 54-55 0.8541632365288621 1105 56-57 1.0886841014759696 1105 58-59 0.9094737477596375 1105 60-61 1.015124421793363 1105 62-63 0.663189858052057 1105 64-65 0.7801483050981837 1105 66-67 0.9033474583819512 1105 68-69 0.9467549161070039 1105 70-71 0.8798396633622438 1105 72-73 0.9781988358468219 1105 74-75 0.9180722311055902 1105 76-77 0.8868171384063359 1105 78-79 0.8713491651844869 1105 80-81 1.2797709998419613 1105 82-83 0.8449330986918859 1105 84-85 1.139303486799939 1105 86-87 1.5895452062437805 1105 88-89 1.9025163660767461 1105 90-91 1.4366675257168424 1105 92-93 1.9574788945599941 1105 94-95 1.907183835748043 1105 96-97 1.9505643621745143 1105 98-99 1.7346979014202262 1105 100-101 1.971572416963287 1106 1 0.2695833531042986 1106 2 0.23162737649999698 1106 3 0.5769876624775243 1106 4 0.34067766747512707 1106 5 0.4499434307200616 1106 6 0.14913701969485516 1106 7 0.3333857824166202 1106 8 0.16226911819196488 1106 9 0.5862050186056962 1106 10-11 0.47706062735748844 1106 12-13 0.1759336249070742 1106 14-15 0.40178558867525993 1106 16-17 0.4811878657313855 1106 18-19 0.6060682194255946 1106 20-21 0.4406184627162233 1106 22-23 0.7159635833320905 1106 24-25 0.40871528149752834 1106 26-27 0.7552521922150675 1106 28-29 0.9004916742836784 1106 30-31 0.7392354386592928 1106 32-33 0.6570360937113477 1106 34-35 0.5853264943902872 1106 36-37 0.7821546782820477 1106 38-39 0.85731168447969 1106 40-41 1.0050652926171892 1106 42-43 1.033002686850871 1106 44-45 1.387712454646973 1106 46-47 1.1108821126540604 1106 48-49 0.7499708234701359 1106 50-51 0.21419783111318225 1106 52-53 0.5115566393536781 1106 54-55 0.8434794758451076 1106 56-57 0.3760942222194217 1106 58-59 0.9636387185912731 1106 60-61 0.9278906678929424 1106 62-63 0.7519837801793159 1106 64-65 0.8695527278359378 1106 66-67 0.7927111810790066 1106 68-69 0.30688244290119826 1106 70-71 0.3018651687210827 1106 72-73 0.25696019794151326 1106 74-75 0.11160092463428839 1106 76-77 -0.3553481037589066 1106 78-79 0.11198815582347876 1106 80-81 0.5320773354816275 1106 82-83 0.35809277851823396 1106 84-85 0.5664504139468676 1106 86-87 0.42396387399578117 1106 88-89 0.40004044693415963 1106 90-91 0.6876171933331783 1106 92-93 0.09256911298355064 1106 94-95 0.5412904698546761 1106 96-97 0.01805859633541118 1106 98-99 0.3017851351741285 1106 100-101 0.6278605732514428 1107 1 -0.10036851967243265 1107 2 0.2910540347967121 1107 3 0.1557698344800329 1107 4 0.15465548710266575 1107 5 0.2672692310967122 1107 6 0.12277167129560951 1107 7 0.5456686867899165 1107 8 0.5299192542036835 1107 9 0.5830662866534055 1107 10-11 0.5767153668427412 1107 12-13 0.41656974124940405 1107 14-15 0.3952465637746556 1107 16-17 0.7542052333815192 1107 18-19 0.5386901068497423 1107 20-21 0.22237196763357048 1107 22-23 0.5940761591847803 1107 24-25 0.6226198640461789 1107 26-27 0.5594999427905023 1107 28-29 0.05162161778650187 1107 30-31 0.4941004731853411 1107 32-33 0.8384548005537837 1107 34-35 0.616923062710022 1107 36-37 0.1825522509960038 1107 38-39 0.5928735174991502 1107 40-41 0.11921051128218352 1107 42-43 0.2713370664281882 1107 44-45 0.6243205316505254 1107 46-47 0.9883669421162935 1107 48-49 1.0069806581635845 1107 50-51 1.1382405178677288 1107 52-53 1.1101651348548245 1107 54-55 0.8312384212311699 1107 56-57 1.0838259652619016 1107 58-59 1.1372629077521914 1107 60-61 1.4316048340364809 1107 62-63 1.0888743430585777 1107 64-65 1.059079825555127 1107 66-67 1.1449815305244968 1107 68-69 0.9472360733678222 1107 70-71 1.162139284644919 1107 72-73 1.2178306729362838 1107 74-75 1.2434276666305166 1107 76-77 0.7184120971199377 1107 78-79 0.8310716460934096 1107 80-81 1.2619371387877614 1107 82-83 1.3465317824939618 1107 84-85 1.40229473284203 1107 86-87 1.582731398582517 1107 88-89 1.2359893902277364 1107 90-91 0.7334949920358369 1107 92-93 1.5229938880410927 1107 94-95 1.8305246192583162 1107 96-97 1.8106407264881632 1107 98-99 0.9647376356972508 1107 100-101 1.2756948482043633 1108 1 0.06877710467900044 1108 2 -0.10693397396611459 1108 3 -0.20664801401529687 1108 4 -0.4030235167022411 1108 5 -0.18894545816882413 1108 6 -0.0419060642053708 1108 7 0.23424242136849216 1108 8 -0.37735043333234586 1108 9 -0.20423337897555882 1108 10-11 -0.04174133787304868 1108 12-13 -0.28813163082485005 1108 14-15 -0.08183749018591158 1108 16-17 -0.0014588966772848266 1108 18-19 -0.09596629808510926 1108 20-21 0.047792813938194456 1108 22-23 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-3.068400116653663 2316 70-71 -3.3990129394340194 2316 72-73 -3.412749417777494 2316 74-75 -3.682608778026122 2316 76-77 -3.754930785031732 2316 78-79 -3.89766233974251 2316 80-81 -3.9813985817501596 2316 82-83 -3.6966368337231152 2316 84-85 -3.5057978906958027 2316 86-87 -3.1697154861155035 2316 88-89 -3.393039017506805 2316 90-91 -4.1738416833839125 2316 92-93 -4.881000700961852 2316 94-95 -4.82790619159551 2316 96-97 -4.222899497909065 2316 98-99 -4.906830757714065 2316 100-101 -4.406568221740731 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 566.0 3 140.0 4 137.0 5 127.0 6 148.0 7 198.0 8 258.0 9 367.0 10 489.0 11 787.0 12 1093.0 13 1517.0 14 1780.0 15 2438.0 16 3413.0 17 4536.0 18 3655.0 19 1979.0 20 1575.0 21 1460.0 22 1592.0 23 1706.0 24 1952.0 25 2071.0 26 2493.0 27 2848.0 28 3362.0 29 4113.0 30 4980.0 31 6047.0 32 7724.0 33 9910.0 34 13774.0 35 20691.0 36 25129.0 37 27105.0 38 19599.0 39 2513.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.20930207303963 20.810212082052843 15.055871078436695 15.924614766470837 2 16.09987670213517 25.18046570744187 34.47017223679708 24.24948535362588 3 24.613112368458207 27.040323193709888 33.18491328287052 15.161651154961392 4 11.630939766892167 18.931724943624854 40.165185970060044 29.272149319422937 5 10.264670411456013 41.914840448070926 32.85835352404959 14.962135616423472 6 23.96627773069987 38.67305477695935 16.597136214501447 20.76353127783934 7 21.77446517237258 39.47076782668217 20.86561131694173 17.889155684003523 8 25.283672726680173 26.478112795554665 20.009994405305726 28.228220072459436 9 23.995349569998208 20.467324753217543 25.93592620158745 29.6013994751968 10-11 25.34054024690017 25.66213518395285 25.153667513207395 23.843657055939595 12-13 30.946955603053766 26.430400786526924 25.036868449569667 17.585775160849646 14-15 24.434697591048586 27.724939572526548 31.404361641454603 16.436001194970263 16-17 18.107049537436918 30.901549844363686 30.093218819771515 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17.97278438740436 29.688475719351214 29.445750682833854 22.89298921041057 44-45 20.030784432958345 26.569626026147763 30.570516440081658 22.82907310081223 46-47 22.86766681776603 29.42257424452582 30.02054770351591 17.68921123419225 48-49 20.95225168978528 28.29664214555227 30.053747387279785 20.697358777382664 50-51 16.888996246407217 30.151419335752493 32.76970636210907 20.189878055731217 52-53 20.46825375281631 32.1466380737805 26.057439127012135 21.327669046391055 54-55 19.08199053161918 27.628778665740093 28.759935284920086 24.52929551772064 56-57 22.5142047382609 28.313104193417903 26.39353029484503 22.779160773476164 58-59 25.046085514080858 27.57393365314915 26.256118717370235 21.123862115399756 60-61 21.82757516031123 28.575849880275612 30.91821276734375 18.678362192069415 62-63 20.729733985654015 28.995780911475162 29.672626966328743 20.601858136542084 64-65 20.094245641274814 32.406711201713364 27.893669635368173 19.605373521643653 66-67 21.876349063698886 28.97334046498747 25.52978325382625 23.620527217487393 68-69 20.548064210494882 28.79807574840302 28.36940852610689 22.284451514995208 70-71 23.451965118835343 29.61072164276932 26.539214169591073 20.398099068804264 72-73 21.84399357346013 26.71193469245967 31.52804012245164 19.91603161162856 74-75 18.80545324760274 33.769857317725226 27.46002752136706 19.96466191330498 76-77 19.659662485511635 29.283467110388468 27.73894608834443 23.317924315755473 78-79 19.753602571481316 29.675198453636238 29.40534494554063 21.16585402934182 80-81 21.641142844734457 28.698963665875887 27.92592029618853 21.733973193201127 82-83 19.450492764640405 28.559715472538212 30.683354600494123 21.30643716232726 84-85 19.510857465559653 29.737075027557708 28.365162712655913 22.386904794226727 86-87 19.0597722058564 30.843435646118134 27.601102317309913 22.495689830715556 88-89 19.05321844355673 30.033918373221596 25.41841259840809 25.49445058481358 90-91 19.591195053938034 27.103691897483795 27.849022381636363 25.45609066694181 92-93 21.84361228097329 29.39553651191902 28.66076350138618 20.10008770572151 94-95 20.263738173602285 28.61173569698352 28.90556261609151 22.218963513322688 96-97 16.469907690302186 29.78298123117618 31.209042493964155 22.538068584557475 98-99 19.55008747242717 30.780243184213674 26.11106281715546 23.558606526203697 100-101 19.23129162999424 28.01365844117743 28.625137182036486 24.12991274679184 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1934.0 1 1179.0 2 400.5 3 364.5 4 325.0 5 332.5 6 497.0 7 870.0 8 1172.5 9 1149.5 10 855.0 11 589.5 12 522.5 13 521.5 14 563.5 15 549.5 16 536.0 17 543.0 18 557.0 19 598.5 20 653.5 21 745.5 22 862.5 23 909.0 24 969.0 25 1254.5 26 1845.5 27 2577.5 28 2698.5 29 2692.0 30 3104.5 31 3409.0 32 3608.0 33 3869.5 34 4439.0 35 4813.5 36 4814.5 37 4718.0 38 3835.5 39 2649.5 40 2180.5 41 2150.5 42 2198.5 43 2443.0 44 2789.0 45 2799.0 46 3626.0 47 9815.0 48 22370.0 49 21702.0 50 9086.5 51 3368.0 52 2065.0 53 1876.0 54 1745.0 55 1677.0 56 1965.0 57 2274.0 58 2518.0 59 2755.0 60 3060.0 61 3249.0 62 2907.0 63 1841.5 64 955.5 65 746.0 66 574.5 67 382.5 68 284.5 69 209.0 70 157.5 71 161.5 72 159.0 73 169.5 74 134.5 75 116.5 76 99.5 77 44.5 78 19.5 79 9.5 80 6.5 81 6.5 82 4.0 83 2.0 84 1.5 85 0.5 86 1.0 87 1.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05318225232265347 2 0.08954154727793696 3 0.059694364851957976 4 0.128614222453764 5 0.10527915255708951 6 0.0976816879395676 7 0.10527915255708951 8 0.0917122514543718 9 0.1101632369540679 10-11 0.10283711035860033 12-13 0.09306894156464357 14-15 0.09062689936615438 16-17 0.10256577233654597 18-19 0.08438612485890423 20-21 0.07597464617521923 22-23 0.06946253364591473 24-25 0.058337674741686205 26-27 0.05508161847703395 28-29 0.06674915342537119 30-31 0.048026829903620734 32-33 0.0458561257271859 34-35 0.045584787705131545 36-37 0.04260006946253365 38-39 0.04965485803594686 40-41 0.06322175913866458 42-43 0.0613223929842841 44-45 0.04775549188156638 46-47 0.036087956933229144 48-49 0.041786055396370585 50-51 0.02631978813927238 52-53 0.041786055396370585 54-55 0.04341408352869671 56-57 0.04911218199183815 58-59 0.05616697056525136 60-61 0.05372492836676218 62-63 0.05915168880784926 64-65 0.039344013197881396 66-67 0.06322175913866458 68-69 0.0523682382564904 70-71 0.025234436051054965 72-73 0.019536337587913517 74-75 0.027947816271598504 76-77 0.039344013197881396 78-79 0.05318225232265347 80-81 0.03527394286706608 82-83 0.05806633671963185 84-85 0.0613223929842841 86-87 0.062136407050447165 88-89 0.08330077277068682 90-91 0.06810584353564296 92-93 0.07244725188851264 94-95 0.08140140661630632 96-97 0.0887275332117739 98-99 0.1161326734392637 100-101 0.11396196926282887 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 184272.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.221173048536944 #Duplication Level Percentage of deduplicated Percentage of total 1 77.35093000538266 21.055830511417902 2 10.217100934989334 5.562429452114266 3 3.6123681744781804 2.9499869757749413 4 1.9397539921452922 2.112095163671095 5 1.136340982037838 1.5466267257098203 6 0.8213552361396305 1.3414951810367284 7 0.5920934590618209 1.1282234957020056 8 0.4505492314746516 0.9811582877485456 9 0.37080600466497876 0.9084396978379786 >10 2.8288909710731445 15.607905704610575 >50 0.390741811367397 7.636537292697751 >100 0.23125535774805128 11.316966223843014 >500 0.01395506469169275 2.689502474602761 >1k 0.04186519407507825 21.656572892246245 >5k 0.0019935806702418212 3.506229920986368 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6461 3.506229920986368 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4734 2.569028392810628 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3387 1.8380437613961969 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2395 1.2997091256403577 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2263 1.2280758878180082 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2157 1.170552227142485 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2113 1.1466744812017018 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2075 1.126052791525571 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2016 1.094034904923157 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1960 1.0636450464530693 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1854 1.0061213857775464 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1830 0.9930971607189372 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1795 0.9741034991751324 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1745 0.946969696969697 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1376 0.7467222366935834 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1302 0.706564209429539 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1288 0.698966744812017 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1230 0.6674915342537119 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1160 0.6295042111661023 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1133 0.6148519579751671 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1085 0.5888035078579491 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1009 0.5475601285056872 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 916 0.4970912564035773 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 842 0.4569332291395329 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 760 0.4124337935226188 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 658 0.35708083702353044 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 618 0.3353737952591821 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 597 0.3239775983328992 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 565 0.3066119649214205 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 485 0.2631978813927238 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 480 0.26048450117218025 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 449 0.24366154380481025 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 406 0.2203264739081358 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 352 0.19102196752626552 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 352 0.19102196752626552 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 338 0.1834245029087436 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 328 0.1779977424676565 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 327 0.1774550664235478 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 322 0.17474168620300426 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 307 0.16660154554137363 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 284 0.15411999652687333 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 280 0.15194929235043847 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 279 0.1514066163063298 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 274 0.14869323608578622 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 270 0.14652253190935138 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 268 0.14543717982113397 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 268 0.14543717982113397 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 261 0.14163844751237303 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 251 0.13621168707128592 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 245 0.13295563080663367 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 245 0.13295563080663367 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 234 0.12698619432143787 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 229 0.12427281410089433 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 228 0.12373013805678562 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 221 0.11993140574802466 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 219 0.11884605365980723 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 215 0.11667534948337241 No Hit GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT 214 0.1161326734392637 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 211 0.11450464530693757 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 209 0.11341929321872016 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 208 0.11287661717461145 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 206 0.11179126508639402 No Hit CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA 202 0.1096205609099592 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 200 0.10853520882174177 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 200 0.10853520882174177 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 196 0.10636450464530695 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 194 0.10527915255708951 No Hit CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT 194 0.10527915255708951 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 192 0.10419380046887211 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 187 0.10148042024832855 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.426760441087088E-4 0.0 18-19 0.0 0.0 0.0 8.140140661630633E-4 0.0 20-21 0.0 0.0 0.0 0.0010853520882174177 0.0 22-23 0.0 0.0 0.0 0.0024420421984891897 0.0 24-25 0.0 0.0 0.0 0.0051554224190327345 0.0 26-27 0.0 0.0 0.0 0.01112485890422853 0.0 28-29 0.0 0.0 0.0 0.02197837978640271 0.0 30-31 0.0 0.0 0.0 0.04151471737431622 0.0 32-33 0.0 0.0 0.0 0.06566380133715377 0.0 34-35 0.0 0.0 0.0 0.09713901189545888 0.0 36-37 0.0 0.0 0.0 0.12888556047581834 0.0 38-39 0.0 0.0 0.0 0.17636971433533039 0.0 40-41 0.0 0.0 0.0 0.22412520621689674 0.0 42-43 0.0 0.0 0.0 0.26645393765737607 0.0 44-45 0.0 0.0 0.0 0.29820048623773554 0.0 46-47 0.0 0.0 0.0 0.3483980203177911 0.0 48-49 0.0 0.0 0.0 0.38150125900842236 0.0 50-51 0.0 0.0 0.0 0.41324780758878177 0.0 52-53 0.0 0.0 0.0 0.47185682035252235 0.0 54-55 0.0 0.0 0.0 0.5657397759833289 0.0 56-57 0.0 0.0 0.0 0.7320699835026483 0.0 58-59 0.0 0.0 0.0 0.9051836415733264 0.0 60-61 0.0 0.0 0.0 1.1306655379004948 0.0 62-63 0.0 0.0 0.0 1.2931970131110533 0.0 64-65 0.0 0.0 0.0 1.4622406008509161 0.0 66-67 0.0 0.0 0.0 1.740904749500738 0.0 68-69 0.0 0.0 0.0 2.13732959972215 0.0 70-71 0.0 0.0 0.0 2.506077971694017 0.0 72-73 0.0 0.0 0.0 2.876183033776157 0.0 74-75 0.0 0.0 0.0 3.2707085178431883 0.0 76-77 0.0 0.0 0.0 3.5930580880437613 0.0 78-79 0.0 0.0 0.0 3.949324911001129 0.0 80-81 0.0 0.0 0.0 4.283613354172093 0.0 82-83 0.0 0.0 0.0 4.6485629938352 0.0 84-85 0.0 0.0 0.0 5.251204740817922 0.0 86-87 0.0 0.0 0.0 6.004981766084918 0.0 88-89 0.0 0.0 0.0 6.792947382130763 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGCG 130 0.0 51.18253 2 TTTGCGT 135 0.0 49.273487 3 ATGGGAC 50 2.8128705E-5 47.51372 7 TTGCGTT 140 0.0 47.513718 4 TAGGCAT 80 4.1800376E-9 47.513718 5 TTAGGCA 90 1.1841621E-8 42.234417 4 GCTTTGC 175 0.0 40.73711 1 ATGGGTG 50 0.0016100054 38.010975 5 CATTCCG 90 5.6505814E-7 36.955116 9 AGGCATT 90 5.6505814E-7 36.955116 6 CTTTAGG 105 4.598587E-8 36.210766 2 TGGGTGC 55 0.0025703807 34.555435 6 TAGGACC 55 0.0025703807 34.555435 4 TAATACC 85 1.6215881E-5 33.539097 4 GTCACTA 75 3.0584112E-4 31.684422 1 GCATTCC 105 1.8859664E-6 31.675812 8 CCTTTAG 125 2.1268352E-7 30.417044 1 ATAAATC 80 4.4715832E-4 29.696075 3 TACCTCC 145 2.3641405E-8 29.499289 2 ATACCTT 100 4.9211463E-5 28.508232 6 >>END_MODULE