##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936903_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 184272 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.848018147086915 31.0 31.0 34.0 30.0 34.0 2 31.778870365546585 31.0 31.0 34.0 30.0 34.0 3 31.91252604845012 31.0 31.0 34.0 30.0 34.0 4 35.70662933055483 37.0 35.0 37.0 33.0 37.0 5 35.65486888946774 37.0 35.0 37.0 33.0 37.0 6 35.80268841712252 37.0 35.0 37.0 35.0 37.0 7 35.75735326039767 37.0 35.0 37.0 33.0 37.0 8 35.75499804636624 37.0 35.0 37.0 33.0 37.0 9 37.28892072588348 39.0 37.0 39.0 34.0 39.0 10-11 37.21656301554224 39.0 37.0 39.0 33.5 39.0 12-13 37.161785295649906 39.0 37.0 39.0 33.0 39.0 14-15 37.98411315880872 40.0 37.0 41.0 33.0 41.0 16-17 37.96611802118608 39.0 37.0 41.0 33.0 41.0 18-19 38.16664767300512 40.0 37.0 41.0 33.5 41.0 20-21 38.28745007380394 40.0 37.5 41.0 34.0 41.0 22-23 38.18646077537554 40.0 37.0 41.0 33.5 41.0 24-25 37.98218665885213 40.0 37.0 41.0 33.0 41.0 26-27 37.67528164886689 39.5 36.5 41.0 33.0 41.0 28-29 37.48434108274724 39.0 36.0 41.0 33.0 41.0 30-31 37.221083506989665 39.0 35.5 41.0 33.0 41.0 32-33 36.83471444386559 39.0 35.0 41.0 32.0 41.0 34-35 36.52243694104367 38.0 35.0 40.0 31.0 41.0 36-37 36.14788736216028 38.0 35.0 40.0 30.5 41.0 38-39 35.792369432143786 38.0 35.0 40.0 30.0 41.0 40-41 35.56110532256664 38.0 35.0 40.0 30.0 41.0 42-43 35.036340301293734 38.0 35.0 40.0 27.0 41.0 44-45 34.31296941477815 38.0 34.0 40.0 23.5 41.0 46-47 33.477885951202566 38.0 33.0 40.0 13.5 41.0 48-49 32.667505101154816 38.0 33.0 40.0 2.0 41.0 50-51 32.15659188590779 37.0 33.0 40.0 2.0 41.0 52-53 31.90045693322914 37.0 32.0 40.0 2.0 41.0 54-55 31.656570178866023 37.0 31.0 40.0 2.0 41.0 56-57 31.235141529912305 36.0 31.0 40.0 2.0 41.0 58-59 30.91921181731354 36.0 31.0 39.0 2.0 41.0 60-61 30.38062483719719 35.0 29.5 39.0 2.0 40.0 62-63 30.097795649908832 35.0 29.0 39.0 2.0 40.0 64-65 29.709538074151254 35.0 29.0 38.0 2.0 40.0 66-67 29.190107558391944 35.0 28.0 37.5 2.0 39.0 68-69 28.683769102196752 34.0 27.5 37.0 2.0 39.0 70-71 28.076954176434835 34.0 26.0 36.0 2.0 38.5 72-73 27.759751888512632 34.0 26.0 35.5 2.0 37.5 74-75 27.42941412694278 34.0 26.0 35.0 2.0 37.0 76-77 26.153192020491446 31.5 24.5 34.0 2.0 35.5 78-79 26.835655986802117 33.0 26.0 35.0 2.0 36.0 80-81 26.601659503342887 33.0 25.0 35.0 2.0 36.0 82-83 26.258020751931927 33.0 24.5 35.0 2.0 35.0 84-85 25.908008270382915 33.0 23.0 35.0 2.0 35.0 86-87 25.705055570026918 33.0 21.0 35.0 2.0 35.0 88-89 25.2601697490666 32.5 19.5 35.0 2.0 35.0 90-91 24.74911272466788 32.0 16.5 34.0 2.0 35.0 92-93 24.339590388121906 32.0 8.5 34.0 2.0 35.0 94-95 23.788440457584443 31.0 2.0 34.0 2.0 35.0 96-97 23.08845619518972 31.0 2.0 34.0 2.0 35.0 98-99 22.478173569505948 31.0 2.0 34.0 2.0 35.0 100-101 19.80836209516367 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14290192069209695 1101 2 0.07289023333657951 1101 3 0.019636279910443477 1101 4 0.07749101914267698 1101 5 -0.049284495002019924 1101 6 -0.035156320209651426 1101 7 0.24798169524497382 1101 8 0.17482313907807168 1101 9 0.29900515003861017 1101 10-11 -0.0607666426567377 1101 12-13 0.29990396459629665 1101 14-15 -0.06691744644110287 1101 16-17 0.0928547166334539 1101 18-19 0.30969899012048074 1101 20-21 0.007466399632818366 1101 22-23 -0.34647036224172467 1101 24-25 0.06915638986045991 1101 26-27 0.29863964952999567 1101 28-29 0.42334966958079434 1101 30-31 0.23871871011244394 1101 32-33 0.23656164308891192 1101 34-35 0.3403383226109753 1101 36-37 0.3968866093319008 1101 38-39 0.5961564834087767 1101 40-41 0.11705358670324273 1101 42-43 0.035109477976384085 1101 44-45 3.297985532313419E-4 1101 46-47 -0.35731028588421765 1101 48-49 -0.9286743623738758 1101 50-51 -0.5555961302012662 1101 52-53 -0.5631068657830767 1101 54-55 -0.9518811211464246 1101 56-57 -0.7904164405902634 1101 58-59 -1.1278310560581524 1101 60-61 -1.082225012950321 1101 62-63 -0.7017435318079102 1101 64-65 -0.863285243838714 1101 66-67 -1.0737996640691918 1101 68-69 -1.1929570940644503 1101 70-71 -1.3966888475463541 1101 72-73 -0.9035927879337358 1101 74-75 -0.8310234485797352 1101 76-77 -0.6286603207946335 1101 78-79 -0.7715989798991423 1101 80-81 -0.5980590211370718 1101 82-83 -0.01991318518617291 1101 84-85 -0.04050198276745576 1101 86-87 -0.18178911671972386 1101 88-89 -0.01613625335160407 1101 90-91 -0.433763919446708 1101 92-93 -0.4788529734792384 1101 94-95 -0.68645853598413 1101 96-97 -0.38069955123197374 1101 98-99 0.19111877237990882 1101 100-101 0.2676142456391162 1102 1 0.5384596424578731 1102 2 0.41055211886784093 1102 3 0.47298539563249165 1102 4 0.2635375307705843 1102 5 0.3589107424300977 1102 6 0.35396079439834693 1102 7 0.3691347379966814 1102 8 0.3467351290516163 1102 9 0.7302821309604894 1102 10-11 0.5317169947196732 1102 12-13 0.5445425943136044 1102 14-15 0.6984181614129668 1102 16-17 0.4342243030420363 1102 18-19 0.9112342916105263 1102 20-21 0.6580199437074583 1102 22-23 0.41035319993624597 1102 24-25 0.7244620575947636 1102 26-27 0.42659884741330245 1102 28-29 0.7920831328867379 1102 30-31 1.144041784893119 1102 32-33 1.0846155353044864 1102 34-35 1.0552979382633865 1102 36-37 1.4422841862710456 1102 38-39 1.3192277826707226 1102 40-41 1.9396061559832845 1102 42-43 1.767876502067729 1102 44-45 1.923303618355483 1102 46-47 2.4626834475790815 1102 48-49 2.543770283218663 1102 50-51 2.2778532501078814 1102 52-53 2.489357971983246 1102 54-55 2.4673153695930203 1102 56-57 2.6537939757862823 1102 58-59 2.602098619474507 1102 60-61 2.579016457596957 1102 62-63 2.820781186197941 1102 64-65 2.8196976276276544 1102 66-67 2.8927265857567335 1102 68-69 2.915018814583366 1102 70-71 2.8231621481366993 1102 72-73 2.504463193127396 1102 74-75 2.333020556433496 1102 76-77 2.2407520173198314 1102 78-79 2.3884065903556966 1102 80-81 2.6728468415561437 1102 82-83 2.986370758554255 1102 84-85 3.17073252496127 1102 86-87 2.8183153255585083 1102 88-89 3.2350474258017172 1102 90-91 2.9716810046585707 1102 92-93 3.499144519906082 1102 94-95 3.359774579180886 1102 96-97 3.5696903666067676 1102 98-99 4.3704809782008205 1102 100-101 4.2885897365178245 1103 1 -0.11728089957944121 1103 2 -0.15183755265616128 1103 3 0.15292032346468432 1103 4 0.08434677932550017 1103 5 0.3401159620486638 1103 6 0.18567309234580165 1103 7 0.24771284190447318 1103 8 0.29076614216988617 1103 9 0.20302450747912815 1103 10-11 0.5219561695492061 1103 12-13 0.3289737320381576 1103 14-15 0.38705822233158216 1103 16-17 0.28590821844821335 1103 18-19 -0.24227372126546243 1103 20-21 0.06626126453402037 1103 22-23 0.3074145685285359 1103 24-25 0.16113783897997536 1103 26-27 0.25543827837795874 1103 28-29 0.2186346541217219 1103 30-31 0.784743041339766 1103 32-33 0.5710992153432457 1103 34-35 0.48207444305727165 1103 36-37 0.8037289268476968 1103 38-39 0.671586648753582 1103 40-41 0.5403295646572701 1103 42-43 0.482078156562217 1103 44-45 0.5730042171578802 1103 46-47 0.8144365886956919 1103 48-49 0.9500559777255972 1103 50-51 1.4655760703633192 1103 52-53 1.641792986347589 1103 54-55 1.453314469658789 1103 56-57 1.737695536826056 1103 58-59 1.970552463232078 1103 60-61 1.9207491771289895 1103 62-63 1.2950638347736216 1103 64-65 0.9516764445602632 1103 66-67 1.271391221802567 1103 68-69 1.3983354183700136 1103 70-71 1.5839873186050113 1103 72-73 1.2494183694798942 1103 74-75 0.9737218128801359 1103 76-77 1.0231409965867329 1103 78-79 0.521787227924488 1103 80-81 1.2565753383198448 1103 82-83 1.5297406661379291 1103 84-85 1.040103609382026 1103 86-87 0.6291733782392761 1103 88-89 0.9729415796904703 1103 90-91 1.8064565402925048 1103 92-93 1.0907128283758496 1103 94-95 0.7976791169262043 1103 96-97 0.9843696785032066 1103 98-99 0.4466638725277754 1103 100-101 -0.12082842138602246 1104 1 0.27031144687043707 1104 2 0.2858923475014876 1104 3 0.4200218040989796 1104 4 0.3865321831770032 1104 5 0.1030908314499186 1104 6 0.07742296097428181 1104 7 0.3405075004930822 1104 8 0.2971337969564445 1104 9 0.25585131345112444 1104 10-11 0.42942425399790807 1104 12-13 0.37442827749270435 1104 14-15 0.46017806406754147 1104 16-17 0.4162442204265133 1104 18-19 0.32644131072737537 1104 20-21 0.20838979224568277 1104 22-23 0.152628008607671 1104 24-25 0.038096820155210764 1104 26-27 0.11089869691216592 1104 28-29 0.3980263267129871 1104 30-31 0.03515261136850967 1104 32-33 -0.09966830343515198 1104 34-35 -0.4414518776128844 1104 36-37 -0.6819593053553277 1104 38-39 -0.9506815964320339 1104 40-41 -0.9717102486406191 1104 42-43 -0.6339615330918988 1104 44-45 -0.36549845663891034 1104 46-47 -0.5782560637725496 1104 48-49 -0.32543782462981596 1104 50-51 -0.23237762504021475 1104 52-53 0.06905953143120058 1104 54-55 0.18438924323610806 1104 56-57 0.36707329949181755 1104 58-59 -0.19036991938360615 1104 60-61 -0.4446954768229716 1104 62-63 0.10421965685021917 1104 64-65 0.03685155506689597 1104 66-67 0.04796690725278552 1104 68-69 0.45893632715181454 1104 70-71 0.594512495570175 1104 72-73 0.45503797938376067 1104 74-75 0.24284796044899082 1104 76-77 0.42441143644948554 1104 78-79 0.4601508024662344 1104 80-81 0.24298997774366526 1104 82-83 0.16378598158413027 1104 84-85 0.22512107406626924 1104 86-87 -0.07244326421381686 1104 88-89 -0.37091177878376413 1104 90-91 -0.322148448734108 1104 92-93 -0.1204398407843037 1104 94-95 0.0506388399641331 1104 96-97 -0.29056772683202325 1104 98-99 -0.12364307316169132 1104 100-101 0.21141787702824644 1105 1 0.4224828049241438 1105 2 0.07959864120777738 1105 3 0.3140395510579026 1105 4 0.21958148681454048 1105 5 0.20160995938081072 1105 6 0.2177826013339228 1105 7 0.26190962777756255 1105 8 0.425270366269487 1105 9 0.37017816307209017 1105 10-11 0.3381919568832572 1105 12-13 0.6146880177524423 1105 14-15 0.14120292156298575 1105 16-17 0.3977039371803457 1105 18-19 0.4173210192541319 1105 20-21 0.43721978578155785 1105 22-23 0.44869958409100974 1105 24-25 0.19073829632609574 1105 26-27 0.44842881385404354 1105 28-29 0.12578386329405333 1105 30-31 0.3904884519984648 1105 32-33 0.5318913134185763 1105 34-35 0.7480497971829152 1105 36-37 0.5415965505534643 1105 38-39 0.17854212102697886 1105 40-41 -0.675432996481014 1105 42-43 -0.18326517063082548 1105 44-45 -0.284138639984981 1105 46-47 -0.12587021432786827 1105 48-49 -0.004479371318417691 1105 50-51 0.24455720483579313 1105 52-53 0.307644475898492 1105 54-55 0.07003301123281958 1105 56-57 0.6196589491362907 1105 58-59 0.27988041851378753 1105 60-61 0.6493428378035766 1105 62-63 0.34757923844509264 1105 64-65 0.39157036566805914 1105 66-67 0.14422359174476185 1105 68-69 0.2847134911642719 1105 70-71 0.018529654881451307 1105 72-73 -0.19914607185911493 1105 74-75 -0.07163167756005606 1105 76-77 0.3802203334348562 1105 78-79 -0.216334476960224 1105 80-81 0.28115769238196364 1105 82-83 0.49877452245502596 1105 84-85 0.4647050195325697 1105 86-87 0.8592280056927493 1105 88-89 0.8388343574329618 1105 90-91 1.5364635275249263 1105 92-93 1.5532834947037415 1105 94-95 1.1957290438778685 1105 96-97 1.1856178522947296 1105 98-99 1.2658057133459195 1105 100-101 0.5093405425979718 1106 1 0.49547154457955145 1106 2 0.23202029362943222 1106 3 0.374072111090463 1106 4 0.4055128394125589 1106 5 0.3663772908148033 1106 6 0.37820859509324833 1106 7 0.44838364758491167 1106 8 0.5354318097642619 1106 9 0.5647243576182888 1106 10-11 0.5743539430452458 1106 12-13 0.5450231147542084 1106 14-15 0.6776633746901126 1106 16-17 0.8892586787350965 1106 18-19 0.5252100438098282 1106 20-21 0.4104595090212797 1106 22-23 0.7015485869400138 1106 24-25 0.426391531979327 1106 26-27 0.46369132911655697 1106 28-29 0.28851619269304507 1106 30-31 -0.39783741966073904 1106 32-33 0.39242584061977226 1106 34-35 0.28352666599310794 1106 36-37 0.7343112432681664 1106 38-39 0.46964916213401864 1106 40-41 0.4425632048485184 1106 42-43 0.2927896387573199 1106 44-45 0.35078199493565876 1106 46-47 -0.33645900824999586 1106 48-49 -0.4175847844238305 1106 50-51 -0.315644938699684 1106 52-53 -0.5969139286599088 1106 54-55 -0.01662415875768275 1106 56-57 -0.13692089077688507 1106 58-59 -0.24945752749082217 1106 60-61 -0.24978889466149212 1106 62-63 -0.8034518292526407 1106 64-65 -0.6681230273586678 1106 66-67 -0.22306035887252307 1106 68-69 -0.07219194907450444 1106 70-71 -0.4619343255825292 1106 72-73 -0.3925390985854733 1106 74-75 -0.5329327221944382 1106 76-77 -0.5769089570277686 1106 78-79 -0.525044285670031 1106 80-81 -0.7277963165720429 1106 82-83 -0.18905616537566416 1106 84-85 -0.2593147878205748 1106 86-87 -0.19083304436830417 1106 88-89 0.06346466539660156 1106 90-91 0.6922768500049159 1106 92-93 0.3183764264633382 1106 94-95 0.47676282491164557 1106 96-97 0.5417092917195028 1106 98-99 1.0732606041341448 1106 100-101 0.8725884058458355 1107 1 0.24991808151196437 1107 2 0.14978551647940108 1107 3 -0.0013202304035750956 1107 4 0.020599677663241778 1107 5 0.028858981545084816 1107 6 0.34347329482123 1107 7 0.07152656451314243 1107 8 0.18211521633467243 1107 9 -0.23784940258259013 1107 10-11 0.032765744677384134 1107 12-13 0.11993418401555545 1107 14-15 0.10646647157230404 1107 16-17 0.1888506435812971 1107 18-19 0.021809750861507382 1107 20-21 -0.041360955511052566 1107 22-23 0.15697859321748098 1107 24-25 0.31528042086821984 1107 26-27 0.16096063231806568 1107 28-29 -0.046020321221995175 1107 30-31 -0.6804279197653642 1107 32-33 0.02964073913410914 1107 34-35 0.12904874174117253 1107 36-37 0.345579290976886 1107 38-39 0.09755248918988713 1107 40-41 -0.02060900691126477 1107 42-43 -0.017840200121106875 1107 44-45 0.12385167478500136 1107 46-47 -0.12250211350214357 1107 48-49 -0.49024642911937377 1107 50-51 -0.6639918732048145 1107 52-53 -0.6634550460484832 1107 54-55 -0.9321399570418407 1107 56-57 -1.0569878503918666 1107 58-59 -1.1103070776059099 1107 60-61 -0.8868991687774113 1107 62-63 -0.7681934069885692 1107 64-65 -1.2647436592900334 1107 66-67 -0.7766803630574977 1107 68-69 -0.9389004655005344 1107 70-71 -0.9459267926258441 1107 72-73 -0.7829450412513062 1107 74-75 -1.1185677922927795 1107 76-77 -0.9743770465862518 1107 78-79 -1.1965759868627472 1107 80-81 -1.0033769819832195 1107 82-83 -0.898723459788723 1107 84-85 -0.7511017725454074 1107 86-87 -1.0943170368353456 1107 88-89 -1.4536937359425899 1107 90-91 -0.5027430286307819 1107 92-93 -0.47221784011962953 1107 94-95 -0.7837519271285274 1107 96-97 -0.26609568840185815 1107 98-99 -0.8425167551460397 1107 100-101 -0.5290123474498323 1108 1 0.013927074939843465 1108 2 0.02151386883729245 1108 3 0.0777758147941654 1108 4 0.13088446954609623 1108 5 0.04658641150011533 1108 6 0.035678990658887244 1108 7 0.022898638766569945 1108 8 0.023779000492403668 1108 9 0.13312949745199631 1108 10-11 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-1.185746972380823 2316 88-89 -1.0512110431758224 2316 90-91 -1.5775023188352861 2316 92-93 -1.5167392056006506 2316 94-95 -1.6559966951718152 2316 96-97 -1.7331124784074312 2316 98-99 -1.5502831273908093 2316 100-101 -1.7850798257660578 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 7.0 5 16.0 6 43.0 7 73.0 8 137.0 9 285.0 10 463.0 11 805.0 12 1248.0 13 1716.0 14 2048.0 15 3090.0 16 4068.0 17 5386.0 18 4324.0 19 2138.0 20 1531.0 21 1489.0 22 1504.0 23 1735.0 24 1807.0 25 2137.0 26 2430.0 27 3002.0 28 3751.0 29 4399.0 30 5560.0 31 6702.0 32 8186.0 33 10746.0 34 14210.0 35 20310.0 36 25881.0 37 28373.0 38 14041.0 39 628.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.34673939591305 20.8336725555646 16.546446307905235 15.273141740617113 2 16.048994122658968 26.227702193013396 34.40659477822942 23.316708906098217 3 25.38707419125508 26.204366489930376 34.05438846890653 14.354170849908012 4 11.768185468990817 18.818785681724446 40.20665552347668 29.20637332580806 5 9.667826190773372 41.748837310265856 33.572292981532634 15.011043517428135 6 23.911934531562036 39.39285404185118 16.487040896066684 20.2081705305201 7 21.344534166883737 39.29408700182339 21.36407050447165 17.99730832682122 8 25.842233220456716 26.301879829816794 19.94714335330381 27.90874359642268 9 23.589584961361464 20.27220630372493 26.006121385777547 30.13208734913606 10-11 25.075431970131113 25.53073717113832 25.8661109663975 23.527719892333074 12-13 31.280915827547002 25.935784620978396 25.686422948871364 17.09687660260324 14-15 24.185714595814883 27.287108187896152 32.636808630719806 15.89036858556916 16-17 17.614179039680472 29.629026656247287 31.653479638794824 21.103314665277416 18-19 16.914398280802292 29.72155292176782 35.539582790657285 17.824466006772596 20-21 20.837584387956674 23.65711897887859 34.71574011765472 20.789556515510018 22-23 18.710384648780064 24.439686984457758 36.46104671355388 20.3888816532083 24-25 22.141029432913744 25.32690423924045 35.01586018077079 17.516206147075025 26-27 20.467631560381832 22.07832028176199 40.895647124584166 16.55840103327201 28-29 17.148710434558453 29.306578751000583 36.69556487172182 16.849145942719147 30-31 18.671725107396885 24.50765686186712 36.221312209458524 20.599305821277472 32-33 18.3803158942967 25.629984180864547 37.58957619369351 18.400123731145246 34-35 21.439367010639366 24.276259914201287 36.20016443396457 18.08420864119478 36-37 20.08710163213806 23.156552294897363 38.7778637035831 17.97848236938147 38-39 18.81140835248564 25.93552343455338 37.22801238410254 18.025055828858434 40-41 18.386884071538468 27.09257616089914 35.89676796127306 18.62377180628933 42-43 17.652278203486226 27.888455944602423 32.758536479475545 21.700729372435802 44-45 20.20594779399794 24.155858251478808 33.46176805774136 22.176425896781897 46-47 22.72142659452654 27.620461168604937 32.394679575190885 17.263432661677637 48-49 21.25613564767205 26.486461552654646 32.20926730395096 20.048135495722345 50-51 16.854161239906226 28.11577450725015 34.92283146652774 20.107232786315883 52-53 20.87393261536708 30.386307086592808 28.06010728792726 20.67965301011285 54-55 19.22894286264731 26.004184157185378 30.46918457777187 24.297688402395444 56-57 22.54656160458453 26.520849613614654 28.49130632977338 22.44128245202744 58-59 25.37099194386483 25.888169230393526 27.628283573787304 21.112555251954337 60-61 21.580561133119875 27.309382970640904 32.45726379768817 18.65279209855104 62-63 20.935627767647823 27.009800729356602 31.31973387166797 20.7348376313276 64-65 20.339281983377788 31.035455835547005 29.392499654043082 19.232762527032126 66-67 22.32921984891899 27.308543891638447 27.30800121559434 23.054235043848227 68-69 20.787205869584096 27.292534948337238 29.687635669011026 22.23262351306764 70-71 23.79050420304877 28.306136071329725 28.128951381389367 19.774408344232135 72-73 22.44779456455674 24.708582964313624 33.118162281844235 19.725460189285403 74-75 19.136050360883488 32.18185271612308 29.095349215824605 19.58674770716883 76-77 19.996146968722613 27.472655688240927 29.383433593548613 23.147763749487847 78-79 19.857059129981767 28.26636710949032 30.961839020578275 20.91473473994964 80-81 21.53185739133266 27.1476367109594 29.92106541340938 21.399440484298555 82-83 19.548547798905965 26.964487279673527 32.13673265607363 21.35023226534688 84-85 18.930322542132355 28.12522894331974 30.16272273641196 22.781725778135947 86-87 19.02805665599392 28.88478862538666 28.77652357953004 23.31063113908938 88-89 18.575308715672257 28.57278526522007 27.173491288669165 25.678414730438508 90-91 19.476914561083618 24.840724581054094 29.720196231657546 25.962164626204743 92-93 21.227044803334202 27.64093296865503 30.429745159329684 20.70227706868108 94-95 19.77267300512286 26.81389467743336 30.66418121038465 22.74925110705913 96-97 15.927899669508497 27.84024615918945 32.891230850215166 23.340623321086877 98-99 19.132199673849044 28.703726315389606 27.979790579609865 24.18428343115149 100-101 18.254635835264367 26.2160079448204 30.222606921327827 25.306749298587405 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2352.0 1 1458.5 2 527.0 3 474.0 4 370.0 5 309.5 6 475.5 7 882.5 8 1199.5 9 1243.0 10 953.5 11 575.5 12 472.5 13 463.0 14 467.0 15 470.0 16 481.0 17 492.5 18 542.5 19 627.5 20 677.0 21 760.5 22 890.0 23 948.5 24 1046.5 25 1288.0 26 1888.5 27 2656.5 28 2785.5 29 2853.5 30 3211.0 31 3407.0 32 3611.5 33 3802.5 34 4350.5 35 4716.0 36 4667.0 37 4647.0 38 3775.5 39 2665.0 40 2258.0 41 2161.0 42 2281.0 43 2649.5 44 2957.5 45 2946.0 46 3702.5 47 9997.5 48 22929.5 49 22018.0 50 9035.0 51 3252.5 52 1828.5 53 1570.0 54 1475.0 55 1521.0 56 1835.5 57 2075.0 58 2450.0 59 2861.5 60 2929.5 61 2879.0 62 2521.0 63 1593.0 64 906.0 65 734.0 66 548.5 67 395.0 68 311.5 69 223.0 70 164.0 71 153.5 72 141.5 73 177.0 74 167.0 75 127.5 76 97.0 77 43.0 78 17.5 79 11.0 80 7.0 81 3.5 82 3.0 83 2.5 84 2.0 85 1.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01465225319093514 2 0.0027133802205435444 3 0.0027133802205435444 4 0.0021707041764348354 5 5.426760441087088E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.140140661630634E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0021707041764348354 22-23 0.0 24-25 0.0029847182425978987 26-27 0.0016280281323261267 28-29 0.0024420421984891897 30-31 0.013838239124772077 32-33 8.140140661630634E-4 34-35 0.0018993661543804808 36-37 0.0024420421984891897 38-39 0.0018993661543804808 40-41 0.004070070330815316 42-43 0.0021707041764348354 44-45 0.0010853520882174177 46-47 5.426760441087088E-4 48-49 2.713380220543544E-4 50-51 0.0 52-53 8.140140661630634E-4 54-55 0.0029847182425978987 56-57 0.0 58-59 0.0013566901102717722 60-61 0.0010853520882174177 62-63 0.0 64-65 2.713380220543544E-4 66-67 0.0 68-69 0.0 70-71 5.426760441087088E-4 72-73 0.0 74-75 0.0010853520882174177 76-77 8.140140661630634E-4 78-79 0.0 80-81 0.0029847182425978987 82-83 0.0 84-85 8.140140661630634E-4 86-87 0.0010853520882174177 88-89 2.713380220543544E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 5.426760441087088E-4 98-99 8.140140661630634E-4 100-101 5.426760441087088E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 184272.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.383671963184856 #Duplication Level Percentage of deduplicated Percentage of total 1 76.35489043292357 19.381674915342536 2 10.467129877071086 5.313883823912477 3 3.771245323356494 2.8718416254232872 4 1.9754142169962587 2.005730659025788 5 1.2335649385355425 1.5656203872536252 6 0.8615713522180651 1.3121906746548582 7 0.6328166755745591 1.1244247633932447 8 0.5152324959914485 1.0462794130415907 9 0.44468198824158206 1.015889554571503 >10 3.008017103153394 15.486346270730225 >50 0.41261357562800643 7.363028566466961 >100 0.2586851950828434 11.682187201528176 >500 0.021378941742383754 4.233415820092038 >1k 0.040619989310529125 21.45632543197013 >5k 0.0021378941742383755 4.1411608925935575 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7631 4.1411608925935575 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3906 2.119692628288617 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3120 1.6931492576191716 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2849 1.5460840496657116 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2731 1.482048276460884 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2506 1.3599461665364245 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2339 1.2693192671702702 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2170 1.1776070157158982 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2031 1.1021750455847876 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2026 1.0994616653642442 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1975 1.0717851871147 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1904 1.0332551879829817 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1755 0.952396457410784 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1702 0.9236346270730225 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1670 0.9062689936615439 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1666 0.9040982894851091 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1386 0.7521489971346704 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1348 0.7315273074585396 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1229 0.6669488582096033 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1225 0.6647781540331683 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 959 0.5204263263002518 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 940 0.5101154814621863 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 905 0.49112181991838155 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 798 0.43305548319874965 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 770 0.4178605539637058 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 735 0.398866892419901 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 701 0.38041590692020494 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 687 0.372818442302683 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 674 0.3657636537292698 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 632 0.342971259876704 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 477 0.25885647303985415 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 428 0.2322653468785274 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 408 0.22141182599635323 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 407 0.22086914995224452 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 382 0.2073022488495268 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 365 0.19807675609967873 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 356 0.19319267170270035 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 352 0.19102196752626552 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 350 0.1899366154380481 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 314 0.1704002778501346 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 302 0.1638881653208301 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 294 0.1595467569679604 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 286 0.15520534861509075 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 278 0.15086394026222108 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 276 0.14977858817400364 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 274 0.14869323608578622 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 266 0.14435182773291658 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 263 0.14272379960059042 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 260 0.14109577146826432 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 258 0.14001041938004688 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 256 0.13892506729182946 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 250 0.1356690110271772 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 248 0.1345836589389598 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 231 0.12535816618911175 No Hit CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA 230 0.12481549014500302 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 228 0.12373013805678562 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 225 0.12210210992445951 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 225 0.12210210992445951 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 217 0.11776070157158983 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 215 0.11667534948337241 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 215 0.11667534948337241 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 211 0.11450464530693757 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 208 0.11287661717461145 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 208 0.11287661717461145 No Hit CTTTAATACCTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAAT 204 0.11070591299817661 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 203 0.1101632369540679 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 198 0.10744985673352436 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 195 0.10582182860119822 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 195 0.10582182860119822 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 192 0.10419380046887211 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 189 0.10256577233654597 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 189 0.10256577233654597 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 185 0.10039506816011115 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.426760441087088E-4 0.0 18-19 0.0 0.0 0.0 8.140140661630633E-4 0.0 20-21 0.0 0.0 0.0 0.0010853520882174177 0.0 22-23 0.0 0.0 0.0 0.0024420421984891897 0.0 24-25 0.0 0.0 0.0 0.0051554224190327345 0.0 26-27 0.0 0.0 0.0 0.010853520882174176 0.0 28-29 0.0 0.0 0.0 0.021435703742294 0.0 30-31 0.0 0.0 0.0 0.04205739341842493 0.0 32-33 0.0 0.0 0.0 0.0678345055135886 0.0 34-35 0.0 0.0 0.0 0.09849570200573066 0.0 36-37 0.0 0.0 0.0 0.12942823651992708 0.0 38-39 0.0 0.0 0.0 0.17908309455587393 0.0 40-41 0.0 0.0 0.0 0.2298233046800382 0.0 42-43 0.0 0.0 0.0 0.2737800642528436 0.0 44-45 0.0 0.0 0.0 0.3066119649214205 0.0 46-47 0.0 0.0 0.0 0.3565381609794217 0.0 48-49 0.0 0.0 0.0 0.3893700616479986 0.0 50-51 0.0 0.0 0.0 0.4205739341842494 0.0 52-53 0.0 0.0 0.0 0.4791829469479899 0.0 54-55 0.0 0.0 0.0 0.5744225926890683 0.0 56-57 0.0 0.0 0.0 0.7426521663627681 0.0 58-59 0.0 0.0 0.0 0.9182078666319354 0.0 60-61 0.0 0.0 0.0 1.148302509334028 0.0 62-63 0.0 0.0 0.0 1.310019970478423 0.0 64-65 0.0 0.0 0.0 1.4817769384388295 0.0 66-67 0.0 0.0 0.0 1.760712425110706 0.0 68-69 0.0 0.0 0.0 2.155237909177737 0.0 70-71 0.0 0.0 0.0 2.528870365546583 0.0 72-73 0.0 0.0 0.0 2.9049448641139186 0.0 74-75 0.0 0.0 0.0 3.3057111226882 0.0 76-77 0.0 0.0 0.0 3.631859425197534 0.0 78-79 0.0 0.0 0.0 3.990839628375445 0.0 80-81 0.0 0.0 0.0 4.323500043414084 0.0 82-83 0.0 0.0 0.0 4.693333767474169 0.0 84-85 0.0 0.0 0.0 5.30031692280976 0.0 86-87 0.0 0.0 0.0 6.058164018407571 0.0 88-89 0.0 0.0 0.0 6.846400972475471 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 90 0.0 58.051075 8 GCACCAG 20 5.385434E-4 47.496338 94-95 ATACCTT 40 5.378381E-4 47.496338 6 TAATACC 45 9.6076593E-4 42.218964 4 GTCCGCA 25 0.0016150031 37.997066 90-91 CGCACCA 25 0.0016150031 37.997066 92-93 CACCAGT 25 0.0016150031 37.997066 94-95 GGGATTC 55 0.002575004 34.542793 7 TCCGCAC 35 2.0515651E-4 33.925953 90-91 GCAGAAA 155 3.6379788E-11 33.70708 9 ATATTAA 75 3.069886E-4 31.664227 1 CTTCGCG 30 0.0039489903 31.664225 48-49 TCGGATT 30 0.0039489903 31.664225 76-77 TTCGCGA 30 0.0039489903 31.664225 48-49 AATACCT 60 0.0039439104 31.664225 5 CCGCACC 30 0.0039489903 31.664225 92-93 TCGCGAT 30 0.0039489903 31.664225 50-51 AGAGCAC 60 0.0039439104 31.664225 3 GGGGTGA 160 1.884473E-9 29.685211 9 TACCTCC 80 4.481193E-4 29.685211 2 >>END_MODULE