##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936902_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 179512 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.848962743437763 33.0 31.0 34.0 30.0 34.0 2 31.89866972681492 33.0 31.0 34.0 30.0 34.0 3 31.855057043540263 33.0 31.0 34.0 30.0 34.0 4 35.45003119568608 37.0 35.0 37.0 33.0 37.0 5 35.49045746245376 37.0 35.0 37.0 33.0 37.0 6 35.66770466598333 37.0 35.0 37.0 33.0 37.0 7 35.657460225500245 37.0 35.0 37.0 33.0 37.0 8 35.619952983644545 37.0 35.0 37.0 33.0 37.0 9 37.16982708676857 39.0 37.0 39.0 34.0 39.0 10-11 37.00254300548153 39.0 37.0 39.0 33.0 39.0 12-13 36.97998462498329 39.0 37.0 39.0 33.0 39.0 14-15 37.88523886982486 40.0 37.0 41.0 33.0 41.0 16-17 37.908560430500465 40.0 37.5 41.0 33.0 41.0 18-19 37.97600996033691 40.0 37.5 41.0 33.0 41.0 20-21 37.91630086010963 40.0 37.5 41.0 33.0 41.0 22-23 37.79045133472971 40.0 37.0 41.0 33.0 41.0 24-25 37.51025279647043 40.0 36.5 41.0 32.5 41.0 26-27 37.47226369267793 39.5 36.5 41.0 32.5 41.0 28-29 37.45640959935826 39.5 36.0 41.0 33.0 41.0 30-31 37.26206047506574 39.0 36.0 41.0 33.0 41.0 32-33 36.836161370827575 39.0 35.0 41.0 32.0 41.0 34-35 36.51284315254691 39.0 35.0 41.0 31.0 41.0 36-37 36.14765029635902 38.5 35.0 40.5 30.5 41.0 38-39 35.81513213601319 38.0 35.0 40.0 30.0 41.0 40-41 35.64564485939658 38.0 35.0 40.0 30.0 41.0 42-43 35.17377111279468 38.0 35.0 40.0 27.5 41.0 44-45 34.61362193056732 38.0 35.0 40.0 24.5 41.0 46-47 33.7738201345871 38.0 33.5 40.0 18.0 41.0 48-49 33.01152290654664 38.0 33.0 40.0 7.0 41.0 50-51 32.083855118320784 36.5 32.0 39.5 2.0 40.5 52-53 32.285841615045236 37.0 33.0 39.5 2.0 40.5 54-55 32.5671125050136 37.5 33.0 40.0 2.0 41.0 56-57 32.32420395293908 37.0 33.0 40.0 2.0 41.0 58-59 32.0586924551005 36.5 32.0 40.0 2.0 41.0 60-61 31.52917910780338 36.0 31.0 39.5 2.0 41.0 62-63 31.26614376754757 35.0 31.0 39.0 2.0 41.0 64-65 30.939563928873838 35.0 31.0 39.0 2.0 40.5 66-67 30.395848188421944 35.0 30.0 38.5 2.0 40.0 68-69 29.92521112794688 35.0 29.5 37.0 2.0 39.0 70-71 29.3569092874014 35.0 29.0 37.0 2.0 39.0 72-73 28.958986028789163 35.0 29.0 36.0 2.0 38.5 74-75 28.62713077677258 34.0 29.0 36.0 2.0 37.0 76-77 28.276363140068632 34.0 28.5 35.0 2.0 37.0 78-79 27.815831253620928 34.0 26.5 35.0 2.0 36.5 80-81 27.345469940728197 34.0 26.0 35.0 2.0 36.0 82-83 26.90232964927136 33.5 25.5 35.0 2.0 36.0 84-85 26.538008601096305 33.0 24.5 35.0 2.0 35.0 86-87 26.409053433753733 33.5 24.0 35.0 2.0 35.0 88-89 25.957877467801595 33.0 22.0 35.0 2.0 35.0 90-91 25.49455746691029 33.0 20.0 35.0 2.0 35.0 92-93 25.109240607870227 33.0 17.5 35.0 2.0 35.0 94-95 24.580281541066892 32.0 7.5 35.0 2.0 35.0 96-97 24.100670150184946 32.0 2.0 35.0 2.0 35.0 98-99 23.58707495877713 32.0 2.0 35.0 2.0 35.0 100-101 21.565494228798073 29.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4263120157041307 1101 2 0.45742601599851795 1101 3 0.27760532377421754 1101 4 0.1946035722391528 1101 5 0.5843286806685057 1101 6 0.46913729404489857 1101 7 0.23146833483225038 1101 8 0.516490209023651 1101 9 0.5359761415690869 1101 10-11 0.38997385831672915 1101 12-13 0.5803634019528161 1101 14-15 1.0166572229872983 1101 16-17 0.9235742594921632 1101 18-19 1.171193177394649 1101 20-21 1.181073514744483 1101 22-23 1.066947249970248 1101 24-25 0.9347445752443804 1101 26-27 1.183895145976578 1101 28-29 1.2255506329047563 1101 30-31 1.0749981341520467 1101 32-33 1.3484219038566465 1101 34-35 1.2224515347505331 1101 36-37 1.432094534168229 1101 38-39 2.0132942108621066 1101 40-41 2.0305181908549557 1101 42-43 2.058370635853798 1101 44-45 2.53137950141889 1101 46-47 1.8418709594275597 1101 48-49 1.3284773644699044 1101 50-51 0.89057971436484 1101 52-53 1.2224118810877158 1101 54-55 1.5266927789727944 1101 56-57 1.345334951678005 1101 58-59 1.3762767181523117 1101 60-61 2.1288652419285974 1101 62-63 2.1237088824317887 1101 64-65 1.9319003044719274 1101 66-67 1.458909513687363 1101 68-69 1.340040123350981 1101 70-71 1.4426729410541874 1101 72-73 0.8140196960010719 1101 74-75 1.0003036470874918 1101 76-77 0.953669840489404 1101 78-79 0.8831133858097928 1101 80-81 0.8059620891068313 1101 82-83 1.2444524125206087 1101 84-85 1.175041722874841 1101 86-87 1.3052792738852794 1101 88-89 1.7740435107907224 1101 90-91 1.402350834058275 1101 92-93 0.91076470631738 1101 94-95 1.3005977347868374 1101 96-97 1.836025189246758 1101 98-99 1.080706020084417 1101 100-101 1.1466392692714749 1102 1 -0.06225941286729508 1102 2 -0.2952525554300536 1102 3 -0.14668039051149506 1102 4 -0.1770035706179911 1102 5 0.2552215378113587 1102 6 0.03806586547347024 1102 7 0.07378976340368126 1102 8 -0.05118836240492186 1102 9 0.236868998711941 1102 10-11 0.2644381440310184 1102 12-13 0.089649116238526 1102 14-15 0.5263000801301629 1102 16-17 0.4641992594921618 1102 18-19 0.45967532025179025 1102 20-21 0.6735735147444828 1102 22-23 0.8118579642559638 1102 24-25 0.3977802895300968 1102 26-27 0.37443086026229366 1102 28-29 0.4557292043333234 1102 30-31 0.11053384843776115 1102 32-33 0.4644040467137813 1102 34-35 0.3929872490362456 1102 36-37 0.20602310559679893 1102 38-39 1.0550799251478225 1102 40-41 0.5890003337121001 1102 42-43 0.8441742072823644 1102 44-45 0.9001295014188884 1102 46-47 0.7601745308561334 1102 48-49 1.059280935898478 1102 50-51 0.9047761429362637 1102 52-53 1.2171440239448614 1102 54-55 1.4371392075442273 1102 56-57 1.5303349516780003 1102 58-59 1.2135088610094584 1102 60-61 0.999311670500024 1102 62-63 1.4369231681460697 1102 64-65 1.5222574473290713 1102 66-67 0.815873799401647 1102 68-69 0.8170044090652695 1102 70-71 0.9278515124827571 1102 72-73 1.01982326742964 1102 74-75 0.9171786470874892 1102 76-77 0.4731341262036892 1102 78-79 0.7469526715240811 1102 80-81 0.8680156605354021 1102 82-83 0.3232024125206081 1102 84-85 0.8155774371605524 1102 86-87 0.9919757024567097 1102 88-89 0.2354720822192924 1102 90-91 0.16752940548684592 1102 92-93 0.3101397063173792 1102 94-95 0.36800844907255126 1102 96-97 1.01548947496104 1102 98-99 0.9966881629415596 1102 100-101 1.2611035549857625 1103 1 0.2218176336816633 1103 2 0.385164780043457 1103 3 0.4422120653472561 1103 4 0.27866256100319475 1103 5 0.4443708154999655 1103 6 0.25607549629209103 1103 7 -0.12983784494303308 1103 8 0.32091436632702397 1103 9 0.10556883819830176 1103 10-11 0.24243172348526798 1103 12-13 0.2418802558853983 1103 14-15 0.8325280095041521 1103 16-17 0.8310531920764319 1103 18-19 0.4705260425631863 1103 20-21 0.5609892450815579 1103 22-23 0.5311677555882284 1103 24-25 0.6001237887275295 1103 26-27 0.6761002021563556 1103 28-29 0.3881208576238606 1103 30-31 0.4848998195452978 1103 32-33 0.9301985892498976 1103 34-35 0.8225217594696375 1103 36-37 0.3507743094491218 1103 38-39 1.0080975816486202 1103 40-41 0.7826600447875407 1103 42-43 1.2770152987751402 1103 44-45 1.5669834340031557 1103 46-47 1.518521240326443 1103 48-49 1.6867287689642865 1103 50-51 1.81238448964573 1103 52-53 1.9341745215371589 1103 54-55 1.8738486778492032 1103 56-57 1.8231439404420442 1103 58-59 2.3246966619725384 1103 60-61 2.3001784441757884 1103 62-63 2.1027819161396515 1103 64-65 2.4165913157078798 1103 66-67 2.4564516485188257 1103 68-69 1.5892676514408706 1103 70-71 1.7302431657732882 1103 72-73 1.2405295274617423 1103 74-75 0.8730213437167045 1103 76-77 1.5153973685792934 1103 78-79 0.8887664756974374 1103 80-81 0.7679354037135688 1103 82-83 0.47050578330712867 1103 84-85 1.207134419504051 1103 86-87 1.348572813211124 1103 88-89 1.2161783422513963 1103 90-91 1.1039660025975984 1103 92-93 1.6830610546319846 1103 94-95 1.114958689843018 1103 96-97 0.17247181846024162 1103 98-99 0.6428197841293581 1103 100-101 0.8226926400579906 1104 1 0.15472110661322702 1104 2 0.5136760159985165 1104 3 0.5423780510469456 1104 4 0.4122172086027831 1104 5 0.5917150443048698 1104 6 0.17595547586307703 1104 7 0.41612742574134387 1104 8 0.41705839084183083 1104 9 0.5751806870236322 1104 10-11 0.6604284037712773 1104 12-13 0.3246815837710031 1104 14-15 0.6001799502600278 1104 16-17 0.4079492594921632 1104 18-19 0.411249995576469 1104 20-21 0.36005078747174935 1104 22-23 0.21439043178843775 1104 24-25 0.380767302517107 1104 26-27 0.3066224187038529 1104 28-29 0.4102097238138498 1104 30-31 0.4829526796065906 1104 32-33 0.2095014493111904 1104 34-35 0.18267880747780652 1104 36-37 0.4394808978045859 1104 38-39 0.3564760290439253 1104 40-41 0.5472795544913183 1104 42-43 1.047859272217437 1104 44-45 0.987629501418887 1104 46-47 0.5432914139730158 1104 48-49 0.8673978190153591 1104 50-51 0.801659259819381 1104 52-53 0.37553688108771865 1104 54-55 0.17243141533644035 1104 56-57 0.1896531334961864 1104 58-59 -0.021734645484045245 1104 60-61 0.12630842374677798 1104 62-63 0.6367770642499728 1104 64-65 0.3313321226537447 1104 66-67 0.18618224096008973 1104 68-69 -0.23325533119447073 1104 70-71 -0.16528160440035933 1104 72-73 -0.6425143949080194 1104 74-75 -0.23123044382159819 1104 76-77 -0.7304210686015011 1104 78-79 -0.4580797960083842 1104 80-81 0.13351890728865 1104 82-83 0.07172513979333672 1104 84-85 0.10004172287483826 1104 86-87 -0.38307299884199253 1104 88-89 -0.419706489209279 1104 90-91 -0.13799007503263283 1104 92-93 -0.29520120277353 1104 94-95 -0.21161817430407126 1104 96-97 0.05988882561038977 1104 98-99 0.45599011099351117 1104 100-101 0.9551619965442022 1105 1 0.3086649568806017 1105 2 0.558528957174989 1105 3 0.5452523825977451 1105 4 0.4846771016509166 1105 5 0.5192551512567363 1105 6 0.2478137646331291 1105 7 0.5722771583616648 1105 8 0.5778872678471814 1105 9 0.41502025921614205 1105 10-11 0.4028415053755481 1105 12-13 0.47595163724692924 1105 14-15 0.42033369357553596 1105 16-17 0.49949337713922404 1105 18-19 0.19858288327700535 1105 20-21 0.5435735147444802 1105 22-23 0.3193369558526058 1105 24-25 0.5064357517149674 1105 26-27 0.40485102832952435 1105 28-29 0.6766535740812287 1105 30-31 0.32095401650498445 1105 32-33 0.2691939626801769 1105 34-35 -0.4948278770141741 1105 36-37 -0.33187605406706666 1105 38-39 0.43608832850915746 1105 40-41 -0.08032739738033712 1105 42-43 -0.03960730532267576 1105 44-45 0.09718832494829854 1105 46-47 -0.4112540405724374 1105 48-49 -0.2827358708242116 1105 50-51 -0.7261482268116382 1105 52-53 -0.1910072365593365 1105 54-55 0.31621483779633053 1105 56-57 0.8615114222662328 1105 58-59 0.5900634828581985 1105 60-61 -0.16396564042434747 1105 62-63 0.6112088824317858 1105 64-65 0.16057677506016077 1105 66-67 -0.1657228392538137 1105 68-69 -0.14635693547254647 1105 70-71 -0.41357705894581187 1105 72-73 -0.9016053039989309 1105 74-75 -0.16164488232427487 1105 76-77 0.1533021934305836 1105 78-79 -0.28876161419020363 1105 80-81 -0.21811879324610928 1105 82-83 -0.16620935218527322 1105 84-85 -0.12459063006633997 1105 86-87 0.23340427388528084 1105 88-89 -0.4759564892092776 1105 90-91 0.004924363470038884 1105 92-93 0.20837500043502644 1105 94-95 0.14784038184566128 1105 96-97 -0.48493069310618253 1105 98-99 -0.44263956815087724 1105 100-101 -0.4463754366108752 1106 1 0.25131201570413353 1106 2 0.22498553980804203 1106 3 -0.09263277146387594 1106 4 0.06156785795343467 1106 5 -0.0338260812362563 1106 6 0.16378015118775124 1106 7 0.17045643007034528 1106 8 0.39000211378555605 1106 9 0.029726141569085485 1106 10-11 0.13431909641197137 1106 12-13 0.2202443543337651 1106 14-15 0.26725246108254197 1106 16-17 0.442175449968353 1106 18-19 0.4814610345375101 1106 20-21 0.4638116099825851 1106 22-23 0.39269129758930177 1106 24-25 0.5136136228634314 1106 26-27 0.1812165745480101 1106 28-29 0.2954911090952308 1106 30-31 0.34374813415204386 1106 32-33 0.33666595147569467 1106 34-35 0.39953486808386174 1106 36-37 0.7975707246444159 1106 38-39 0.6900799251478205 1106 40-41 0.7044765241882871 1106 42-43 0.5588170644252273 1106 44-45 0.47572473951412775 1106 46-47 0.6720792927608912 1106 48-49 0.8801142692318038 1106 50-51 0.8008475715076955 1106 52-53 0.5645249763258136 1106 54-55 0.9884487313537491 1106 56-57 0.8679539992970504 1106 58-59 1.1291040991046941 1106 60-61 0.9876450038333537 1106 62-63 0.9445422157651215 1106 64-65 0.7765431616147787 1106 66-67 1.2550404660683157 1106 68-69 1.4891472662081249 1106 70-71 1.0245181791494247 1106 72-73 0.7787518388582129 1106 74-75 0.9906310280398714 1106 76-77 1.3200388881084528 1106 78-79 0.6761193381907482 1106 80-81 0.7989680414877824 1106 82-83 0.831059555377756 1106 84-85 0.6937917228748383 1106 86-87 0.3732852262662334 1106 88-89 0.6680911298383414 1106 90-91 1.1847913102487482 1106 92-93 0.615377801555475 1106 94-95 0.553127496691598 1106 96-97 0.6154894749610413 1106 98-99 0.5719262581796549 1106 100-101 0.37967498355719087 1107 1 0.2115392884314069 1107 2 0.32617601599851653 1107 3 0.21851441468331245 1107 4 0.4463081176936967 1107 5 0.04626049885032302 1107 6 0.19300093040853028 1107 7 0.007036516650430258 1107 8 0.09887657266001781 1107 9 0.308135232478179 1107 10-11 0.5723602219530903 1107 12-13 0.5178634019528161 1107 14-15 0.6001799502600278 1107 16-17 0.5102219867648898 1107 18-19 0.5277272683037424 1107 20-21 0.6725507874717565 1107 22-23 0.30814043178843775 1107 24-25 0.5228127570625603 1107 26-27 0.48275878234021263 1107 28-29 0.4386188147229433 1107 30-31 0.6335208614247705 1107 32-33 0.7833650856748307 1107 34-35 0.7849515347505331 1107 36-37 1.2434581705318664 1107 38-39 1.2740896654075584 1107 40-41 0.8512568272185987 1107 42-43 1.30354109039925 1107 44-45 1.3399022286916136 1107 46-47 1.372836868518469 1107 48-49 1.9441023644699058 1107 50-51 1.7164319870921076 1107 52-53 1.7761050629058985 1107 54-55 1.7832268698818936 1107 56-57 1.953857678950726 1107 58-59 2.1686062636068613 1107 60-61 1.5751720601104076 1107 62-63 1.4691634278863326 1107 64-65 1.572809395381018 1107 66-67 1.2003867864146365 1107 68-69 1.3860628506237092 1107 70-71 1.1330138501450975 1107 72-73 1.4995310596374338 1107 74-75 1.2318377379965852 1107 76-77 1.2894652950348586 1107 78-79 1.1441929312643424 1107 80-81 0.7613598163795565 1107 82-83 1.2933160488842468 1107 84-85 1.5034508137839317 1107 86-87 1.2277224557034607 1107 88-89 1.5859753289725411 1107 90-91 1.637578106785547 1107 92-93 1.3496851608628333 1107 94-95 0.5980409166050187 1107 96-97 0.5598888256103933 1107 98-99 0.18042192917532773 1107 100-101 0.2676619965442022 1108 1 0.5364243752546933 1108 2 0.503142308133345 1108 3 0.3747963350101742 1108 4 0.47529177448634385 1108 5 0.23650564696063725 1108 6 0.3010193165168076 1108 7 0.4825217056187654 1108 8 0.3658581865517405 1108 9 0.352759849434257 1108 10-11 0.3238923976425738 1108 12-13 0.6182847502674278 1108 14-15 0.728595425234495 1108 16-17 0.6231880235371037 1108 18-19 0.6952451436867833 1108 20-21 0.3446971102500953 1108 22-23 0.42723517131856426 1108 24-25 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9 329.0 10 383.0 11 628.0 12 1000.0 13 1410.0 14 1665.0 15 2321.0 16 3268.0 17 4489.0 18 3588.0 19 1829.0 20 1540.0 21 1438.0 22 1536.0 23 1724.0 24 1854.0 25 1990.0 26 2245.0 27 2635.0 28 3128.0 29 3822.0 30 4662.0 31 5843.0 32 7231.0 33 9550.0 34 13198.0 35 19710.0 36 25086.0 37 27587.0 38 19912.0 39 2513.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.048372715113686 20.70775746767722 15.01504681230495 16.228823004904147 2 16.113770486322018 25.18765777463235 34.51732804306516 24.181243695980474 3 24.727396321452254 27.032478422938272 33.05399698004714 15.186128275562341 4 11.690779794016674 18.66612421418699 40.345088947344955 29.29800704445138 5 10.243747456899674 41.699041843406334 32.91398886331079 15.143221836383194 6 24.056277731944974 38.580012932283886 16.698811567704965 20.66489776806618 7 21.701347276183256 39.650165274053926 20.806136043121757 17.842351406641065 8 25.287913754083192 26.43901133817185 20.033557421095466 28.239517486649497 9 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34.336664772537354 19.094494768860514 34-35 21.675896418781896 26.629320526820138 32.47080649833976 19.223976556058208 36-37 20.372196410067854 25.552640082004658 35.44863009882898 18.626533409098506 38-39 19.6025069637883 28.17214484679666 33.61448467966574 18.610863509749304 40-41 18.7443593943243 29.626410847789998 32.88514891198984 18.744080845895866 42-43 17.92968401818344 29.817441394063643 29.57343568945539 22.67943889829753 44-45 20.038549599184442 26.637104546290157 30.51546162030873 22.80888423421667 46-47 22.801769280477753 29.47556654856608 29.84853042761325 17.874133743342917 48-49 20.950863598508164 28.185537893326572 30.040861119544537 20.82273738862072 50-51 16.89841895912023 30.13866140767234 32.702699691368345 20.26021994183909 52-53 20.568687639623178 32.056367373997915 26.00681890351586 21.36812608286305 54-55 19.103117278878216 27.64715222047972 28.741027133780317 24.508703366861745 56-57 22.453175538449965 28.370762257380616 26.325334938027062 22.850727266142357 58-59 25.017967074291764 27.450346806317725 26.277890749045934 21.253795370344577 60-61 21.756015478522016 28.612699854296853 30.86176587760378 18.76951878957735 62-63 20.85342514980068 28.847252052359902 29.62306777314424 20.676255024695177 64-65 20.275717218537512 32.4464958731556 27.960868099757374 19.31691880854952 66-67 21.87328499037505 29.033075832328127 25.593166983783846 23.50047219351298 68-69 20.426374838758207 28.822649352386097 28.367411188160464 22.383564620695225 70-71 23.48544365510517 29.53280401170079 26.594233180108652 20.387519153085385 72-73 21.863342963084715 26.827447298701518 31.355566265597272 19.9536434726165 74-75 18.832286785640658 33.59278801419664 27.497367380027747 20.07755782013495 76-77 19.74914468478709 29.151482732105244 27.77517747094157 23.324195112166095 78-79 19.903759348216177 29.504976427448927 29.46986837265807 21.121395851676827 80-81 21.550724960715044 28.780828940476322 27.98900021174871 21.679445887059927 82-83 19.37140246178878 28.533736759109118 30.70354334876827 21.391317430333828 84-85 19.506562177010235 29.629113723042977 28.303863201384033 22.560460898562756 86-87 19.25955023624855 30.834057680306675 27.518777302308994 22.387614781135774 88-89 19.120621354030277 30.0650772513776 25.467046283966372 25.347255110625756 90-91 19.552554384616027 26.99772405013218 28.013404982575196 25.436316582676593 92-93 21.960787591560166 29.34188127060017 28.692984729059923 20.004346408779746 94-95 20.30779002969806 28.50985942175146 28.979289363859746 22.203061184690736 96-97 16.44087915027362 29.550393990392653 31.218862499024773 22.78986436030895 98-99 19.56993082998445 30.733001510481404 26.10847597442772 23.58859168510643 100-101 19.273978902189214 27.91404802051253 28.533883307088807 24.278089770209448 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1688.0 1 1027.5 2 374.5 3 348.5 4 293.0 5 303.0 6 481.5 7 824.5 8 1070.5 9 1057.0 10 810.0 11 562.0 12 507.5 13 523.0 14 518.5 15 505.0 16 488.5 17 489.0 18 540.0 19 584.0 20 639.0 21 739.5 22 809.5 23 858.0 24 944.0 25 1195.5 26 1818.0 27 2556.5 28 2667.0 29 2715.5 30 3124.5 31 3361.0 32 3579.5 33 3820.5 34 4325.0 35 4683.0 36 4670.5 37 4657.5 38 3811.5 39 2589.5 40 2160.5 41 2101.5 42 2122.0 43 2402.5 44 2702.5 45 2738.5 46 3628.5 47 9657.0 48 21997.5 49 21277.5 50 8780.5 51 3231.0 52 1983.5 53 1772.0 54 1659.0 55 1630.5 56 1883.5 57 2139.0 58 2477.5 59 2737.0 60 2957.5 61 3146.0 62 2780.5 63 1721.0 64 881.0 65 713.5 66 571.0 67 396.0 68 301.5 69 218.5 70 163.5 71 149.5 72 143.5 73 164.5 74 131.0 75 103.5 76 87.0 77 40.5 78 17.0 79 7.5 80 4.0 81 3.0 82 3.5 83 3.0 84 0.5 85 0.5 86 0.5 87 1.5 88 1.5 89 0.0 90 0.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04010873924862962 2 0.03509514684255092 3 0.021725567093007707 4 0.04456526583181069 5 0.05849191140425153 6 0.0646196354561255 7 0.06294843798743259 8 0.06573376710192076 9 0.0679620303935113 10-11 0.07019029368510184 12-13 0.06740496457061367 14-15 0.06545523419047194 16-17 0.04902179241499176 18-19 0.049578858237889385 20-21 0.05319978608672401 22-23 0.029803021525023395 24-25 0.01726904050982664 26-27 0.010584250635055038 28-29 0.015040777218236108 30-31 0.012533981015196757 32-33 0.005570658228976336 34-35 0.011141316457952672 36-37 0.005570658228976336 38-39 0.006684789874771602 40-41 0.005570658228976336 42-43 0.004456526583181068 44-45 0.0016711974686929006 46-47 0.002228263291590534 48-49 8.355987343464503E-4 50-51 0.005570658228976336 52-53 0.00612772405187397 54-55 0.00696332278622042 56-57 0.021447034181558893 58-59 0.009470118989259771 60-61 0.02088996835866126 62-63 0.01309104683809439 64-65 0.009191586077810955 66-67 0.016990507598377825 68-69 0.025346494941842327 70-71 0.02061143544721244 72-73 0.018661705067070725 74-75 0.01838317215562191 76-77 0.025625027853291144 78-79 0.03676634431124382 80-81 0.030081554436472216 82-83 0.027296225321984047 84-85 0.020332902535763628 86-87 0.022282632915905345 88-89 0.01838317215562191 90-91 0.014762244306787289 92-93 0.029803021525023395 94-95 0.021725567093007707 96-97 0.03676634431124382 98-99 0.05514951646686572 100-101 0.060998707607290883 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 179512.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.194839342216675 #Duplication Level Percentage of deduplicated Percentage of total 1 77.07607849563686 20.960715718169258 2 10.22163955917899 5.559516912518383 3 3.8571838256380846 3.146864833548732 4 1.8906960547339096 2.056687018138063 5 1.1737473882584293 1.59599358260172 6 0.782498258838953 1.2767948660813762 7 0.6493506493506493 1.236129061009849 8 0.5059609160555533 1.100762066045724 9 0.3400385103855136 0.8322563394090645 >10 2.802245073538449 15.289785641071349 >50 0.3932975541808349 7.354940059717456 >100 0.24581097136302182 11.51343642764829 >500 0.018435822852226637 3.601430545033201 >1k 0.040968495227170304 20.951245599179998 >5k 0.0020484247613585154 3.523441329827533 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6325 3.523441329827533 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4435 2.4705869245510046 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3176 1.7692410535228842 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2297 1.2795801951958643 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2147 1.1960203217612193 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2070 1.1531262533981015 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2062 1.1486697268149204 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1992 1.1096751192120862 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1986 1.1063327242747003 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1925 1.0723517090779446 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1855 1.0333571014751102 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1766 0.9837782432372209 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1713 0.9542537546236463 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1665 0.92751459512456 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1414 0.7876910735772539 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1373 0.7648513748384509 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1207 0.6723784482374437 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1161 0.6467534203841526 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1147 0.6389544988635857 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1112 0.6194571950621686 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1107 0.6166718659476804 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 970 0.5403538482107045 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 929 0.5175141494719016 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 775 0.4317260127456661 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 748 0.4166852355274299 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 727 0.4049868532465796 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 651 0.36264985070635947 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 596 0.3320112304469896 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 561 0.3125139266455724 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 508 0.28298943803199783 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 477 0.26572039752217125 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 469 0.2612638709389902 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 404 0.22505459245064396 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 357 0.19887249877445518 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 356 0.19831543295155754 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 348 0.1938589063683765 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 333 0.18550291902491198 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 315 0.17547573421275456 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 314 0.17491866838985695 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 299 0.16656268104639244 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 289 0.16099202281741612 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 282 0.15709256205713268 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 276 0.15375016711974687 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 273 0.15207896965105397 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 255 0.14205178483889658 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 252 0.14038058737020367 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 243 0.13536699496412496 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 239 0.13313873167253443 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 237 0.13202460002673916 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 235 0.1309104683809439 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 232 0.129239270912251 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 231 0.12868220508935335 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 229 0.12756807344355808 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 229 0.12756807344355808 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 227 0.12645394179776281 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 220 0.12255448103747939 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 216 0.12032621774588886 No Hit CTTTAATACCTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAAT 212 0.11809795445429831 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 210 0.11698382280850306 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 207 0.11531262533981015 No Hit CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA 205 0.11419849369401489 No Hit GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT 202 0.11252729622532198 No Hit CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT 201 0.11197023040242435 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 198 0.11029903293373144 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 197 0.10974196711083382 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 196 0.10918490128793619 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 196 0.10918490128793619 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 194 0.10807076964214092 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 190 0.10584250635055038 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 190 0.10584250635055038 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 189 0.10528544052765273 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 188 0.10472837470475511 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 184 0.10250011141316458 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 182 0.10138597976736931 No Hit GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC 181 0.10082891394447167 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.7853291144881677E-4 0.0 16-17 0.0 0.0 0.0 5.570658228976335E-4 0.0 18-19 0.0 0.0 0.0 5.570658228976335E-4 0.0 20-21 0.0 0.0 0.0 0.0022282632915905346 0.0 22-23 0.0 0.0 0.0 0.0038994607602834356 0.0 24-25 0.0 0.0 0.0 0.007241855697669237 0.0 26-27 0.0 0.0 0.0 0.012812513926645572 0.0 28-29 0.0 0.0 0.0 0.025625027853291148 0.0 30-31 0.0 0.0 0.0 0.04567939747760595 0.0 32-33 0.0 0.0 0.0 0.06907616203930655 0.0 34-35 0.0 0.0 0.0 0.08829493292927493 0.0 36-37 0.0 0.0 0.0 0.11169169749097554 0.0 38-39 0.0 0.0 0.0 0.1559784304113374 0.0 40-41 0.0 0.0 0.0 0.20527875573777798 0.0 42-43 0.0 0.0 0.0 0.258478541824502 0.0 44-45 0.0 0.0 0.0 0.2905098266411159 0.0 46-47 0.0 0.0 0.0 0.3464949418423281 0.0 48-49 0.0 0.0 0.0 0.37546236463300503 0.0 50-51 0.0 0.0 0.0 0.40693658362672136 0.0 52-53 0.0 0.0 0.0 0.4643143633851776 0.0 54-55 0.0 0.0 0.0 0.5540019608716966 0.0 56-57 0.0 0.0 0.0 0.7166651811578056 0.0 58-59 0.0 0.0 0.0 0.8918623824591114 0.0 60-61 0.0 0.0 0.0 1.1063327242747003 0.0 62-63 0.0 0.0 0.0 1.2391929230357859 0.0 64-65 0.0 0.0 0.0 1.4127189268683988 0.0 66-67 0.0 0.0 0.0 1.7221689914880343 0.0 68-69 0.0 0.0 0.0 2.123534916885779 0.0 70-71 0.0 0.0 0.0 2.5070747359508 0.0 72-73 0.0 0.0 0.0 2.8847653638753954 0.0 74-75 0.0 0.0 0.0 3.305350060163109 0.0 76-77 0.0 0.0 0.0 3.615357190605642 0.0 78-79 0.0 0.0 0.0 3.9576741387762375 0.0 80-81 0.0 0.0 0.0 4.254868755292126 0.0 82-83 0.0 0.0 0.0 4.596350104728375 0.0 84-85 0.0 0.0 0.0 5.198259726369267 0.0 86-87 0.0 0.0 0.0 5.948070323989483 0.0 88-89 0.0 0.0 0.0 6.790353848210705 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACAGAA 25 0.005007118 57.0209 7 CGTCACT 50 2.8066597E-5 47.530666 1 TAATACC 75 1.3473436E-7 44.361954 4 TCAGAGC 45 9.5728744E-4 42.24948 1 TTAATAC 85 3.59958E-7 39.142902 3 GAGCACT 50 0.0016070781 38.024532 4 CCCAGCT 50 0.0016092815 38.013935 8 AATACCT 90 5.633756E-7 36.968296 5 GTCACTA 65 1.3187024E-4 36.562054 2 TGGGAGA 110 6.9341695E-8 34.558125 6 GCACTGG 55 0.0025692265 34.558125 6 ACTACCT 70 2.0387098E-4 33.950478 5 ATACCTT 100 1.2874134E-6 33.26219 6 CTTTGCG 135 1.1777956E-8 31.68711 2 AGAGCAC 60 0.0039297086 31.68711 3 ATGGGCA 60 0.003935077 31.678282 7 CTCACGT 30 0.0039456636 31.669455 12-13 TCAATGC 30 0.0039483556 31.665043 90-91 TTGCGTT 140 1.6783815E-8 30.55543 4 CACTACC 80 4.4618023E-4 29.706667 4 >>END_MODULE