##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936902_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 179512 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.919097330540577 31.0 31.0 34.0 30.0 34.0 2 31.93514082624003 31.0 31.0 34.0 30.0 34.0 3 32.032020143500155 33.0 31.0 34.0 30.0 34.0 4 35.76209946967334 37.0 35.0 37.0 35.0 37.0 5 35.695992468470074 37.0 35.0 37.0 33.0 37.0 6 35.825332011230444 37.0 35.0 37.0 35.0 37.0 7 35.78717300236196 37.0 35.0 37.0 35.0 37.0 8 35.80141717545345 37.0 35.0 37.0 35.0 37.0 9 37.35534671776817 39.0 37.0 39.0 34.0 39.0 10-11 37.248487566290834 39.0 37.0 39.0 33.5 39.0 12-13 37.217158184411076 39.0 37.0 39.0 33.5 39.0 14-15 38.0686137974063 40.0 37.0 41.0 33.0 41.0 16-17 38.059803801417175 39.5 37.0 41.0 33.0 41.0 18-19 38.243056174517584 40.0 37.5 41.0 33.5 41.0 20-21 38.37251827175899 40.0 38.0 41.0 34.0 41.0 22-23 38.280761174740405 40.0 37.5 41.0 34.0 41.0 24-25 38.08506395115647 40.0 37.0 41.0 33.0 41.0 26-27 37.77110722402959 39.5 36.5 41.0 33.0 41.0 28-29 37.61800603859352 39.0 36.0 41.0 33.0 41.0 30-31 37.38035061722893 39.0 36.0 41.0 33.0 41.0 32-33 36.98985861669415 39.0 35.0 41.0 32.5 41.0 34-35 36.65126843887874 38.5 35.0 41.0 31.5 41.0 36-37 36.25792704665983 38.5 35.0 40.5 30.5 41.0 38-39 35.923122131111015 38.0 35.0 40.0 30.5 41.0 40-41 35.65199540977762 38.0 35.0 40.0 30.0 41.0 42-43 35.11712865992246 38.0 35.0 40.0 28.0 41.0 44-45 34.48120459913544 38.0 34.5 40.0 24.0 41.0 46-47 33.56161148001248 38.0 33.5 40.0 13.5 41.0 48-49 32.70912529524489 38.0 33.0 40.0 2.0 41.0 50-51 32.22138074334863 37.0 33.0 40.0 2.0 41.0 52-53 32.014104906635765 37.0 32.5 40.0 2.0 41.0 54-55 31.73492579883239 37.0 32.0 40.0 2.0 41.0 56-57 31.335426043941354 36.0 31.0 40.0 2.0 41.0 58-59 31.00898547172334 36.0 31.0 39.0 2.0 41.0 60-61 30.484959222781765 35.0 30.0 39.0 2.0 40.0 62-63 30.195750144837113 35.0 30.0 39.0 2.0 40.0 64-65 29.819073376710193 35.0 29.0 38.0 2.0 40.0 66-67 29.233260172021925 35.0 28.0 37.5 2.0 39.5 68-69 28.747228597531084 34.0 27.5 37.0 2.0 39.0 70-71 28.226352555817996 34.0 26.0 36.0 2.0 38.5 72-73 27.864922679263785 34.0 26.0 35.5 2.0 37.5 74-75 27.48082300904675 34.0 26.0 35.0 2.0 37.0 76-77 26.144310686750746 31.5 25.0 34.0 2.0 35.5 78-79 26.819438254824192 33.0 26.0 35.0 2.0 36.0 80-81 26.515492000534785 33.0 25.0 35.0 2.0 36.0 82-83 26.276162596372387 33.0 24.5 35.0 2.0 35.0 84-85 25.9451512990775 33.0 23.5 35.0 2.0 35.0 86-87 25.740552163643656 33.0 22.0 35.0 2.0 35.0 88-89 25.274769931815143 32.5 19.5 35.0 2.0 35.0 90-91 24.753125139266455 32.0 16.5 34.0 2.0 35.0 92-93 24.36513157894737 32.0 8.5 34.0 2.0 35.0 94-95 23.830351174294755 31.0 2.0 34.0 2.0 35.0 96-97 23.121069900619457 31.0 2.0 34.0 2.0 35.0 98-99 22.47807945986898 31.0 2.0 34.0 2.0 35.0 100-101 19.676801550871254 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29401462097429487 1101 2 0.31567788225676807 1101 3 0.4133041046507131 1101 4 0.2357623744220092 1101 5 0.2882781643455985 1101 6 0.44142611512872065 1101 7 0.18364533425857843 1101 8 0.26457804060412116 1101 9 0.39884531964410286 1101 10-11 0.24037597769307695 1101 12-13 0.1222334734892101 1101 14-15 0.33445610211798993 1101 16-17 0.5035342618261396 1101 18-19 0.2523551943960598 1101 20-21 0.262653734242015 1101 22-23 0.22759736875853065 1101 24-25 0.36965884354924583 1101 26-27 0.26552892631075053 1101 28-29 0.07072379938316686 1101 30-31 0.04423440224864095 1101 32-33 -0.07415602624751472 1101 34-35 0.5569542847681817 1101 36-37 0.6173063895189088 1101 38-39 -0.13700647855011283 1101 40-41 -0.06624186971538393 1101 42-43 -0.23142378362089033 1101 44-45 -0.9588771403546517 1101 46-47 -0.5240181418556986 1101 48-49 -0.6723948463167275 1101 50-51 -0.8937778260075859 1101 52-53 -0.9016422646579301 1101 54-55 -0.5095025953097476 1101 56-57 -0.5189666886196598 1101 58-59 -0.38492039428720304 1101 60-61 -0.135911674324813 1101 62-63 -0.345383827629103 1101 64-65 -0.6258712607281112 1101 66-67 -0.5642793310299261 1101 68-69 -0.47867095023116946 1101 70-71 0.016864396505766877 1101 72-73 0.32484645178133675 1101 74-75 -0.04598807577963626 1101 76-77 -0.33973119945092733 1101 78-79 -0.17187861424287476 1101 80-81 -0.20610379031788284 1101 82-83 -0.12316321769051797 1101 84-85 -1.1142934420894086 1101 86-87 -0.06960944437198435 1101 88-89 -0.19452160929848716 1101 90-91 -1.2823617400708223 1101 92-93 -0.7187168972548434 1101 94-95 -0.5626912924047609 1101 96-97 -0.2907953989708396 1101 98-99 0.3976403371004018 1101 100-101 0.37176966322867244 1102 1 0.3511574781171518 1102 2 0.23549931082819953 1102 3 0.2484826760792842 1102 4 0.40665523156486216 1102 5 0.424885307202743 1102 6 0.3574975437001484 1102 7 0.43507390568714754 1102 8 0.24154232631840244 1102 9 0.42902389107267425 1102 10-11 0.3855545491216503 1102 12-13 0.4295549020606444 1102 14-15 0.3387418164037044 1102 16-17 0.45451640468328236 1102 18-19 0.5161944801103502 1102 20-21 0.23729659138488302 1102 22-23 0.39241879732995955 1102 24-25 0.48635527212067586 1102 26-27 0.22338606916789416 1102 28-29 0.3120630850974493 1102 30-31 0.5765558308200696 1102 32-33 0.7770939737524856 1102 34-35 0.999722141911036 1102 36-37 0.9796278180903428 1102 38-39 1.1686185214498934 1102 40-41 0.9438474159989028 1102 42-43 0.5382190735219652 1102 44-45 0.7189800025024837 1102 46-47 0.9789282867157354 1102 48-49 1.0301944393975546 1102 50-51 0.8245257454209849 1102 52-53 1.299518449627783 1102 54-55 0.9260331189759654 1102 56-57 0.5119261685231962 1102 58-59 0.6490974628556536 1102 60-61 0.9096240399608995 1102 62-63 1.1310447437994675 1102 64-65 1.1995751678433173 1102 66-67 0.9867028118272181 1102 68-69 0.8585611926259702 1102 70-71 1.1614179679343408 1102 72-73 0.9815428803527659 1102 74-75 1.4311547813632224 1102 76-77 1.4488402291205027 1102 78-79 1.2005321000428424 1102 80-81 1.0988069239678318 1102 82-83 0.9769260680237686 1102 84-85 1.4118672721963037 1102 86-87 1.2201226984851594 1102 88-89 0.7197641049872239 1102 90-91 1.057638259929174 1102 92-93 0.7261045313165866 1102 94-95 1.525344421880952 1102 96-97 1.6602760296005918 1102 98-99 2.1940689085289726 1102 100-101 2.5131089489429534 1103 1 0.16704832883946352 1103 2 0.2772649609084574 1103 3 0.11997545296532053 1103 4 0.21265844183773908 1103 5 0.26517423176132837 1103 6 0.1738699353534372 1103 7 0.16117342414622016 1103 8 0.24077186082883628 1103 9 0.044561611778931365 1103 10-11 -0.0636268312956787 1103 12-13 0.1314329116914692 1103 14-15 0.148501045938211 1103 16-17 -0.12732950221879946 1103 18-19 0.14901952023875964 1103 20-21 0.12262564435437895 1103 22-23 -0.10266948517405439 1103 24-25 -0.07412626881030349 1103 26-27 -0.17239242200385263 1103 28-29 0.13073082185507445 1103 30-31 -0.19010548539180405 1103 32-33 0.3447344231906868 1103 34-35 0.370332093756943 1103 36-37 -0.14006720598671052 1103 38-39 -0.07250507405572648 1103 40-41 0.12150391680146555 1103 42-43 0.456918913008316 1103 44-45 0.2071340956004022 1103 46-47 -0.036272355338844875 1103 48-49 -0.09293557665380803 1103 50-51 0.13511964590252745 1103 52-53 0.09130015107241007 1103 54-55 -0.041735741377166846 1103 56-57 -0.07409309311404044 1103 58-59 -0.4841128100175389 1103 60-61 -0.27320100016750715 1103 62-63 -0.1658894456066342 1103 64-65 0.05414980668761871 1103 66-67 -0.08334534226588275 1103 68-69 0.3214343868474785 1103 70-71 -0.05192071585378244 1103 72-73 -0.1183768628254036 1103 74-75 -0.2004824578021065 1103 76-77 0.024313744369298007 1103 78-79 0.22141492508297134 1103 80-81 0.1766560411427882 1103 82-83 -0.23710282443209252 1103 84-85 -0.3148903522017683 1103 86-87 0.29805207248194776 1103 88-89 0.13075928957791305 1103 90-91 0.5530315183561427 1103 92-93 0.18640950723953864 1103 94-95 0.6040924154604106 1103 96-97 0.5778843763100596 1103 98-99 0.7063482022689378 1103 100-101 0.990765449745524 1104 1 -0.28098537902570797 1104 2 -0.3053448450159557 1104 3 -0.4577186226220107 1104 4 -0.33866944375981234 1104 5 -0.08160819929076979 1104 6 0.15733520603781415 1104 7 0.013190788804031683 1104 8 -0.16780832303224713 1104 9 -0.25740468035589714 1104 10-11 -0.10962402230691737 1104 12-13 -0.01753925378351795 1104 14-15 -0.05020298879110641 1104 16-17 0.10779562546250077 1104 18-19 -0.039406169240294275 1104 20-21 -0.03905081121252607 1104 22-23 0.3003246414857941 1104 24-25 0.3409656617310688 1104 26-27 0.20927892631075196 1104 28-29 -0.10228756425319574 1104 30-31 -0.04553832502408994 1104 32-33 -0.1281332989747881 1104 34-35 -0.3791252606863651 1104 36-37 -0.12132997411745094 1104 38-39 -0.8264951149137474 1104 40-41 -0.5142532333517451 1104 42-43 -0.3802874199845334 1104 44-45 0.6411228596453427 1104 46-47 0.16035685814430423 1104 48-49 0.1932301536832668 1104 50-51 -0.6133800987348579 1104 52-53 -0.5519263555670229 1104 54-55 -0.609502595309749 1104 56-57 -0.7178303249832929 1104 58-59 -0.8011135761053865 1104 60-61 -0.5847753106884497 1104 62-63 -0.36015655490183107 1104 64-65 -0.32672353345538596 1104 66-67 -0.6571770583026542 1104 68-69 -0.7335004956857176 1104 70-71 -0.4038742398578634 1104 72-73 -1.1316876391277582 1104 74-75 -0.4380335303250895 1104 76-77 -0.7340493812691058 1104 78-79 -0.7403445233337784 1104 80-81 -0.953546972136067 1104 82-83 -1.1814018540541547 1104 84-85 -1.5253729875439568 1104 86-87 -0.6465980807356217 1104 88-89 -0.4484988820257634 1104 90-91 -0.3795208309799172 1104 92-93 -0.6868987154366621 1104 94-95 -0.6393958378593076 1104 96-97 -0.907272671698113 1104 98-99 -0.6199732992632363 1104 100-101 -0.03703715495315052 1105 1 0.41166167979782387 1105 2 0.3351631763744187 1105 3 0.33940704582718695 1105 4 0.24127708030436423 1105 5 0.4761458114044217 1105 6 0.24069082101107142 1105 7 0.3130570989644639 1105 8 0.3311221582511763 1105 9 0.15877179023233623 1105 10-11 0.1664789188695508 1105 12-13 0.2703952381950998 1105 14-15 0.628573749176816 1105 16-17 0.446365144179083 1105 18-19 0.4951860767490075 1105 20-21 0.4883890283596699 1105 22-23 0.3779650158173524 1105 24-25 0.1301367847257211 1105 26-27 0.79935245572252 1105 28-29 0.533407622912577 1105 30-31 0.5526902846015886 1105 32-33 0.37474103257601143 1105 34-35 0.5549322259446541 1105 36-37 0.6639975659894972 1105 38-39 0.7992067567440131 1105 40-41 0.7946037185199089 1105 42-43 0.72519386343793 1105 44-45 1.1043581537629947 1105 46-47 0.7162392110854796 1105 48-49 0.6597742713303276 1105 50-51 0.8589795269335951 1105 52-53 0.5158209706361916 1105 54-55 0.5511591693961293 1105 56-57 0.10419507608622425 1105 58-59 -0.011574806051907416 1105 60-61 0.021808913910479788 1105 62-63 -0.005677945276165275 1105 64-65 0.17100373927188883 1105 66-67 -0.1743896251475725 1105 68-69 -0.3942959502311716 1105 70-71 -0.2358929564354071 1105 72-73 -0.2587932541010183 1105 74-75 -0.6342233698972812 1105 76-77 -1.1044370818038693 1105 78-79 -0.43198890836051973 1105 80-81 -0.19194937855317917 1105 82-83 -0.29172939416110566 1105 84-85 -1.0953596185599999 1105 86-87 -1.4168520914308083 1105 88-89 -1.4033451387102538 1105 90-91 -1.1676558577178824 1105 92-93 -1.3359963090195492 1105 94-95 -1.7454118806400558 1105 96-97 -1.1694718695590751 1105 98-99 -0.27147730995842423 1105 100-101 0.8521005455816102 1106 1 0.30234795430762773 1106 2 0.5350231203520117 1106 3 0.33205410465071594 1106 4 0.15808380299343838 1106 5 0.2879805452979767 1106 6 0.28368801989062575 1106 7 0.30269295330619883 1106 8 0.30892327869936054 1106 9 0.5640238910726723 1106 10-11 0.40972121578832343 1106 12-13 0.14872156872731068 1106 14-15 0.4707656259275055 1106 16-17 0.369159261826141 1106 18-19 0.4209563848722553 1106 20-21 0.5227727818610717 1106 22-23 0.5171807020918635 1106 24-25 0.5733790816444895 1106 26-27 0.5232670215488469 1106 28-29 0.7196821327164926 1106 30-31 0.4004844022486438 1106 32-33 0.6178082594667771 1106 34-35 0.45650785619675105 1106 36-37 0.7646278180903394 1106 38-39 0.5464756643070316 1106 40-41 0.9877759874274759 1106 42-43 0.8572666925695813 1106 44-45 0.20064666916915286 1106 46-47 0.19904733433478583 1106 48-49 -0.2874246082214924 1106 50-51 -0.348093302198059 1106 52-53 0.21308987819920588 1106 54-55 0.06758073802358666 1106 56-57 0.4170452161422453 1106 58-59 0.6381450819032715 1106 60-61 0.41319546853232936 1106 62-63 0.5099733152280379 1106 64-65 0.8150513583195078 1106 66-67 0.6132504308748317 1106 68-69 0.5545135735783546 1106 70-71 0.6997513012676748 1106 72-73 0.21666192797180983 1106 74-75 0.41234525755369944 1106 76-77 -0.16532643754616672 1106 78-79 -0.02172980471906172 1106 80-81 0.28630692396783175 1106 82-83 0.36418797278567183 1106 84-85 0.24924822457725426 1106 86-87 -0.09952015865770036 1106 88-89 -0.06714065691753746 1106 90-91 -0.3258141210232033 1106 92-93 -0.036871659159604775 1106 94-95 0.0876063266428595 1106 96-97 0.32229983912439764 1106 98-99 -0.029740615280552163 1106 100-101 -0.13105771772371 1107 1 0.09969643915611215 1107 2 -0.22011757228868234 1107 3 0.2581904682870828 1107 4 0.15564873805837465 1107 5 0.08316452798196394 1107 6 0.21983520603781415 1107 7 0.08705442516767192 1107 8 -0.11099014121406015 1107 9 -0.06990468035589714 1107 10-11 0.18014870496581636 1107 12-13 0.3659834734892158 1107 14-15 0.7594561021179871 1107 16-17 0.4572274436443209 1107 18-19 0.24468473985061223 1107 20-21 -0.0788235384852527 1107 22-23 0.3770291869403479 1107 24-25 0.23869293445834217 1107 26-27 0.3513243808562052 1107 28-29 0.2585078902922575 1107 30-31 0.4090071295213704 1107 32-33 0.496866701025219 1107 34-35 0.8083747393136349 1107 36-37 0.7195791167916354 1107 38-39 0.34395943054079936 1107 40-41 0.7556331302846147 1107 42-43 0.7049398527427471 1107 44-45 1.5445319505544362 1107 46-47 1.7029704945079445 1107 48-49 1.9631165173196266 1107 50-51 1.8667335376287753 1107 52-53 2.1185281898875203 1107 54-55 1.9331110410538912 1107 56-57 2.0321696750167 1107 58-59 2.0454773329855236 1107 60-61 1.7845428711297338 1107 62-63 1.6341616269163488 1107 64-65 2.1391855574537075 1107 66-67 1.6496411235155257 1107 68-69 2.064794958859739 1107 70-71 1.942716669233043 1107 72-73 2.1524032699631555 1107 74-75 1.7352619242203637 1107 76-77 1.260268800549074 1107 78-79 1.782382749393495 1107 80-81 1.9015666642275697 1107 82-83 1.8839390550367554 1107 84-85 2.20758155791059 1107 86-87 1.9556746465371084 1107 88-89 1.5401374816106035 1107 90-91 1.9017291690200864 1107 92-93 2.250601284563338 1107 94-95 2.1333314348679657 1107 96-97 2.192159146483707 1107 98-99 1.7976403371004004 1107 100-101 1.5794401177741229 1108 1 0.29064383445744113 1108 2 0.32220878113317397 1108 3 0.17053725071813375 1108 4 0.2070404643096495 1108 5 -0.06628644239597747 1108 6 0.3704991488365863 1108 7 0.4757801657192502 1108 8 0.19021006307603017 1108 9 0.17377509492499854 1108 10-11 0.39142934847959765 1108 12-13 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0.752396857350508 2316 82-83 0.012766186454559403 2316 84-85 0.056027153765512594 2316 86-87 0.120270736975165 2316 88-89 0.2764628466600598 2316 90-91 0.18758644645767575 2316 92-93 0.33027921673479455 2316 94-95 -0.2850034685705616 2316 96-97 -1.2516114611470037 2316 98-99 -1.0903130307752456 2316 100-101 -1.4758177720044934 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 10.0 6 38.0 7 72.0 8 131.0 9 274.0 10 430.0 11 769.0 12 1161.0 13 1692.0 14 2029.0 15 2801.0 16 3990.0 17 5350.0 18 4290.0 19 2020.0 20 1471.0 21 1482.0 22 1511.0 23 1559.0 24 1761.0 25 2031.0 26 2384.0 27 2802.0 28 3429.0 29 4226.0 30 5142.0 31 6327.0 32 7778.0 33 10361.0 34 13904.0 35 19905.0 36 25754.0 37 28133.0 38 13939.0 39 554.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.56266090115793 20.524022333916573 16.515842146909026 15.39747461801647 2 16.20231867587006 25.994016679758662 34.493228374531334 23.310436269839943 3 25.219915209386016 26.198739839889473 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24.42538660368109 36.55326663398547 20.394458309193812 24-25 22.33379665350986 25.113996261869676 35.04817508488787 17.504031999732593 26-27 20.4949220904386 22.227483663225684 40.729681398082484 16.547912848253233 28-29 17.083653664026027 29.15789239117113 36.75530077659302 17.003153168209824 30-31 18.71738506587764 24.594968837327226 36.16207375955288 20.525572337242252 32-33 18.384286287267702 25.59745309505771 37.54122287089442 18.47703774678016 34-35 21.331658361442965 24.254688575001882 36.16630131110189 18.247351752453266 36-37 20.071482893970753 23.192939740978975 38.644220777954516 18.09135658709575 38-39 18.988910832620704 25.804528703422573 37.19731809837911 18.00924236557762 40-41 18.46614966350225 27.222890760796897 35.8760083790168 18.43495119668405 42-43 17.665050125767067 27.765691635306144 32.86768413106516 21.701574107861628 44-45 20.215089105160246 24.125521567404057 33.3677795294891 22.2916097979466 46-47 22.766800180494354 27.545722450935063 32.34804213763252 17.33943523093807 48-49 21.408337720287783 26.31114230723165 32.14119586985647 20.139324102624094 50-51 16.817173990551744 28.193522149924238 34.909528478474016 20.079775381050005 52-53 20.62315986507302 30.508984454428717 28.084811244286357 20.783044436211906 54-55 19.140491171776425 26.104468008656923 30.418724357206724 24.33631646235993 56-57 22.632039262217916 26.287804090000055 28.504938416030217 22.575218231751816 58-59 25.43660763527177 25.861934499105892 27.654045201076272 21.047412664546066 60-61 21.751192120861003 27.146096082713132 32.43042247872008 18.672289317705783 62-63 20.95848745487767 26.88511074468559 31.40764962788003 20.74875217255671 64-65 20.238390377474644 30.939215746355757 29.558679744060434 19.26371413210916 66-67 22.26226951144783 27.216589605035928 27.42855551222773 23.092585371288507 68-69 20.797900935053214 27.334917288039286 29.5412194965698 22.3259622803377 70-71 23.84806939159791 28.063876369754265 28.15830376093993 19.929750477707895 72-73 22.526728657466474 24.810991202803514 33.03071497417669 19.631565165553322 74-75 19.17800587735547 31.969192629629113 29.200428963384912 19.652372529630508 76-77 19.93610152448281 27.473001919182632 29.477141114240112 23.113755442094444 78-79 19.876873363418575 28.089865730681375 31.03264805838765 21.000612847512397 80-81 21.66930344489505 27.070335130799055 29.832546013636975 21.427815410668924 82-83 19.568414660642556 26.908763739477777 32.11040607016117 21.412415529718498 84-85 18.941736161015204 28.064296895454877 30.086735631799723 22.9072313117302 86-87 19.047194616515885 28.780805740006237 28.74710325772093 23.42489638575694 88-89 18.66905092167054 28.496303847675076 27.246519711883955 25.58812551877044 90-91 19.52726999256311 24.700783519626984 29.65815370131552 26.113792786494383 92-93 21.13903566066798 27.634162713809424 30.42144932218827 20.805352303334328 94-95 19.88257019745253 26.670436155900873 30.632856390816187 22.814137255830406 96-97 15.966342082980525 27.82014572841927 32.92398279780739 23.289529390792815 98-99 19.19904852892433 28.746069193338585 27.905733058884806 24.149149218852273 100-101 18.284496685421423 26.286000779900842 30.187176201882902 25.24232633279483 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2086.0 1 1335.5 2 540.5 3 458.5 4 356.5 5 300.5 6 430.0 7 792.5 8 1119.5 9 1154.0 10 881.5 11 570.5 12 448.5 13 424.5 14 475.0 15 509.5 16 475.0 17 496.5 18 561.0 19 587.0 20 628.5 21 745.5 22 867.0 23 939.0 24 1033.0 25 1231.0 26 1805.0 27 2569.5 28 2706.5 29 2786.5 30 3108.0 31 3289.0 32 3562.0 33 3817.0 34 4326.0 35 4657.0 36 4558.0 37 4522.5 38 3768.5 39 2594.5 40 2151.5 41 2168.5 42 2283.0 43 2592.0 44 2889.0 45 2890.5 46 3552.5 47 9652.0 48 22416.5 49 21572.5 50 8826.0 51 3179.5 52 1787.0 53 1536.0 54 1433.5 55 1449.0 56 1763.5 57 2039.0 58 2446.5 59 2736.5 60 2792.0 61 2769.0 62 2419.0 63 1568.5 64 886.5 65 741.0 66 574.5 67 387.0 68 289.5 69 219.0 70 170.5 71 168.5 72 142.5 73 141.0 74 130.5 75 118.0 76 83.5 77 34.5 78 17.0 79 7.0 80 3.5 81 3.5 82 3.0 83 2.5 84 2.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030081554436472216 2 0.007241855697669237 3 0.00612772405187397 4 0.0 5 0.005570658228976336 6 0.0 7 0.0 8 5.570658228976335E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.003342394937385801 20-21 5.570658228976335E-4 22-23 0.0 24-25 0.0058491911404251525 26-27 0.005013592406078703 28-29 0.005570658228976336 30-31 0.028131824056330496 32-33 0.0 34-35 0.0036209278488346184 36-37 0.015876375952582556 38-39 0.00696332278622042 40-41 0.008913053166362137 42-43 0.007520388609118054 44-45 0.002785329114488168 46-47 0.002785329114488168 48-49 0.001392664557244084 50-51 0.004456526583181068 52-53 0.004177993671732252 54-55 0.001392664557244084 56-57 5.570658228976335E-4 58-59 0.0016711974686929006 60-61 0.0 62-63 0.0 64-65 0.009191586077810955 66-67 0.001114131645795267 68-69 0.0019497303801417176 70-71 0.005013592406078703 72-73 0.012812513926645572 74-75 0.005292125317527519 76-77 0.0047350594946298855 78-79 0.01225544810374794 80-81 0.0 82-83 0.00612772405187397 84-85 5.570658228976335E-4 86-87 0.0 88-89 5.570658228976335E-4 90-91 8.355987343464503E-4 92-93 2.7853291144881677E-4 94-95 2.7853291144881677E-4 96-97 0.0 98-99 2.7853291144881677E-4 100-101 0.001114131645795267 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 179512.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.40554391906948 #Duplication Level Percentage of deduplicated Percentage of total 1 76.06893829759242 19.325727527964702 2 10.584133666622812 5.377913454253755 3 3.9643906503530237 3.0215250233967645 4 1.9800026312327326 2.0121217523062525 5 1.2914967328860236 1.640558848433531 6 0.8266456168048063 1.2600828913944473 7 0.6446520194711222 1.14644146352333 8 0.4933561373503486 1.0027184812157406 9 0.33986756128579576 0.7771068229421988 >10 3.0851203788975137 15.883060742457328 >50 0.40345568565539625 7.303132938187976 >100 0.25215980353462264 11.460515174473015 >500 0.021926939437793273 4.24428450465707 >1k 0.04166118493180722 21.531151120816435 >5k 0.002192693943779327 4.01365925397745 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7205 4.01365925397745 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3784 2.1079370738446452 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3100 1.7269040509826643 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2892 1.6110343598199561 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2540 1.4149471901599893 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2355 1.311890012923927 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2310 1.2868220508935335 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2152 1.1988056508757075 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1997 1.1124604483265743 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1987 1.1068897900975978 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1948 1.0851642230045901 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1866 1.0394848255269844 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1723 0.9598244128526227 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1666 0.9280716609474576 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1611 0.8974330406880877 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1605 0.8940906457507019 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1349 0.7514817950889077 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1294 0.7208431748295379 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1284 0.7152725166005615 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1188 0.6617941976023887 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1000 0.5570658228976336 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 914 0.5091581621284371 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 904 0.5035875038994607 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 817 0.45512277730736667 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 737 0.41055751147555597 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 684 0.3810330228619814 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 678 0.37769062792459557 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 645 0.35930745576897366 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 643 0.3581933241231784 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 597 0.33256829626988726 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 439 0.24455189625206117 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 409 0.22783992156513214 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 391 0.21781273675297472 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 385 0.2144703418155889 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 383 0.21335621016979367 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 341 0.18995944560809305 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 335 0.18661705067070725 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 325 0.18104639244173093 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 311 0.17324747092116405 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 303 0.16879094433798297 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 299 0.16656268104639244 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 296 0.16489148357769953 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 278 0.15486429876554214 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 261 0.14539417977628238 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 259 0.1442800481304871 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 255 0.14205178483889658 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 253 0.1409376531931013 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 252 0.14038058737020367 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 249 0.13870938990151077 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 243 0.13536699496412496 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 237 0.13202460002673916 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 237 0.13202460002673916 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 229 0.12756807344355808 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 228 0.12701100762066045 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 227 0.12645394179776281 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 227 0.12645394179776281 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 222 0.12366861268327467 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 219 0.12199741521458177 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 217 0.12088328356878648 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 216 0.12032621774588886 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 214 0.11921208610009358 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 213 0.11865502027719596 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 208 0.11586969116270779 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 207 0.11531262533981015 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 205 0.11419849369401489 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 203 0.1130843620482196 No Hit CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT 202 0.11252729622532198 No Hit CTTTAATACCTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAAT 200 0.11141316457952671 No Hit CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA 198 0.11029903293373144 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 196 0.10918490128793619 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 194 0.10807076964214092 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 194 0.10807076964214092 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 188 0.10472837470475511 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 186 0.10361424305895983 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 184 0.10250011141316458 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 183 0.10194304559026696 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.7853291144881677E-4 0.0 16-17 0.0 0.0 0.0 5.570658228976335E-4 0.0 18-19 0.0 0.0 0.0 5.570658228976335E-4 0.0 20-21 0.0 0.0 0.0 0.0022282632915905346 0.0 22-23 0.0 0.0 0.0 0.0038994607602834356 0.0 24-25 0.0 0.0 0.0 0.008077454432015687 0.0 26-27 0.0 0.0 0.0 0.013648112660992022 0.0 28-29 0.0 0.0 0.0 0.026460626587637592 0.0 30-31 0.0 0.0 0.0 0.04679352912340122 0.0 32-33 0.0 0.0 0.0 0.07158295824234592 0.0 34-35 0.0 0.0 0.0 0.0913587949552119 0.0 36-37 0.0 0.0 0.0 0.11475555951691252 0.0 38-39 0.0 0.0 0.0 0.15764962788003029 0.0 40-41 0.0 0.0 0.0 0.20583582156067562 0.0 42-43 0.0 0.0 0.0 0.2604282722046437 0.0 44-45 0.0 0.0 0.0 0.2930166228441553 0.0 46-47 0.0 0.0 0.0 0.34900173804536744 0.0 48-49 0.0 0.0 0.0 0.3801974241276349 0.0 50-51 0.0 0.0 0.0 0.41139311020990244 0.0 52-53 0.0 0.0 0.0 0.4707206203485004 0.0 54-55 0.0 0.0 0.0 0.5615223494808146 0.0 56-57 0.0 0.0 0.0 0.7283635634386558 0.0 58-59 0.0 0.0 0.0 0.9082958242345915 0.0 60-61 0.0 0.0 0.0 1.1261085609875663 0.0 62-63 0.0 0.0 0.0 1.2606399572173448 0.0 64-65 0.0 0.0 0.0 1.4391795534560363 0.0 66-67 0.0 0.0 0.0 1.7486296180756717 0.0 68-69 0.0 0.0 0.0 2.1472102143589287 0.0 70-71 0.0 0.0 0.0 2.5265720397522173 0.0 72-73 0.0 0.0 0.0 2.899527608182183 0.0 74-75 0.0 0.0 0.0 3.3195552386469984 0.0 76-77 0.0 0.0 0.0 3.6306765007353268 0.0 78-79 0.0 0.0 0.0 3.969929586879986 0.0 80-81 0.0 0.0 0.0 4.268516867953117 0.0 82-83 0.0 0.0 0.0 4.61612594144124 0.0 84-85 0.0 0.0 0.0 5.221935023842417 0.0 86-87 0.0 0.0 0.0 5.968960292348144 0.0 88-89 0.0 0.0 0.0 6.815700343152547 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGACCA 25 0.005015675 56.99632 9 ACTACCT 55 4.9484006E-5 43.179035 4 TCACTAC 45 9.606398E-4 42.219498 2 CATTCCG 50 0.0016126962 37.997547 9 CGCCTCC 25 0.0016148477 37.997547 84-85 TGGGAGA 90 5.665188E-7 36.942062 6 CACTACC 65 1.3242262E-4 36.536102 3 TAGGCAT 60 0.0039433986 31.664623 5 ATGGGGA 155 1.3405952E-9 30.643185 5 TCAACGA 55 2.7279202E-6 30.225325 94-95 TTATCCT 95 3.4761586E-5 29.998066 4 ATCCTAC 80 4.480406E-4 29.685583 6 GTCACTA 65 0.005817083 29.245176 1 TTAGGCA 65 0.0058329534 29.228882 4 TACCTGG 65 0.0058329534 29.228882 2 CTTTAGG 65 0.0058329534 29.228882 2 TAAAGGT 115 3.842657E-6 28.911179 5 TACCTCC 115 3.842657E-6 28.911179 2 TTTGCGT 150 3.3058313E-8 28.498161 3 CCTACTA 85 6.388385E-4 27.939373 8 >>END_MODULE